Factsheet for Os04t0487300-01

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 5
plastid 1
mitochondrion 2
cytosol 1

Predictors

GFP

MS/MS

Papers

nucleus: 23777608

msms PMID: 23777608 doi

H Mujahid, F Tan, J Zhang, BR Nallamilli, K Pendarvis, Z Peng

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, MS 39762, USA.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os01t0911700-02

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G22320.1	Os01t0911700-02	AT3G24650.1	10743655

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
HORVU1Hr1G048500.1	Barley	endoplasmic reticulum	17.37	59.18
Zm00001d003242_P001	Maize	mitochondrion	72.46	58.74
KXG26596	Sorghum	nucleus	72.46	58.74
TraesCS2D01G345000.2	Wheat	nucleus	68.26	55.61
TraesCS2A01G346300.1	Wheat	nucleus	68.26	55.61
TraesCS2B01G364700.1	Wheat	nucleus, plastid	67.07	54.63
GSMUA_AchrUn_...	Banana	nucleus	67.66	54.59
TraesCS2B01G364600.1	Wheat	nucleus, plastid	68.26	54.55
TraesCS2D01G344900.1	Wheat	nucleus	67.66	54.07
TraesCS2A01G346200.1	Wheat	nucleus	67.66	54.07
VIT_11s0016g03530.t01	Wine grape	nucleus	62.28	50.49
HORVU6Hr1G071230.1	Barley	cytosol, endoplasmic reticulum, extracellular, golgi, nucleus, plasma membrane, plastid, vacuole	29.94	50.0
AT3G16680.1	Thale cress	golgi	25.15	48.28
KRH48354	Soybean	nucleus	59.28	48.06
PGSC0003DMT400077392	Potato	nucleus	58.68	47.57
PGSC0003DMT400051454	Potato	nucleus	58.68	47.57
KRH39324	Soybean	nucleus	58.68	47.57
Solyc05g051790.2.1	Tomato	nucleus	52.69	46.81
AT3G22320.1	Thale cress	nucleus	56.89	46.34
CDY24303	Canola	nucleus	56.29	45.63
Bra001864.1-P	Field mustard	nucleus	56.29	45.63
CDX92939	Canola	nucleus	56.29	45.63
CDY35542	Canola	nucleus	56.29	45.63
HORVU7Hr1G020400.1	Barley	cytosol, mitochondrion, nucleus, plastid	30.54	45.54
Os01t0805800-01	Rice	nucleus	56.89	45.24
CDY10635	Canola	nucleus	55.09	44.66
Bra031350.1-P	Field mustard	nucleus	55.09	44.66
HORVU4Hr1G028860.1	Barley	cytosol, endoplasmic reticulum, extracellular, golgi, nucleus, plasma membrane, plastid, vacuole	29.94	44.64
HORVU2Hr1G026650.1	Barley	cytosol, endoplasmic reticulum, extracellular, golgi, nucleus, plasma membrane, plastid, vacuole	29.94	44.64
HORVU1Hr1G052270.3	Barley	cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	29.94	44.64
TraesCS2D01G344800.1	Wheat	mitochondrion, nucleus	54.49	44.61
CDY37407	Canola	nucleus	54.49	44.17
HORVU2Hr1G084270.1	Barley	nucleus	53.89	44.12
TraesCS2B01G364500.1	Wheat	nucleus	53.29	43.63
TraesCS2A01G346100.2	Wheat	nucleus	52.69	43.14
HORVU6Hr1G011700.1	Barley	endoplasmic reticulum, nucleus	46.11	42.31
HORVU2Hr1G084300.6	Barley	nucleus, plastid	65.87	39.86
PGSC0003DMT400069997	Potato	nucleus	49.1	39.61
Solyc03g025570.2.1	Tomato	cytosol	26.95	38.46
Solyc05g053510.1.1	Tomato	nucleus	47.31	38.16
AT5G57980.1	Thale cress	nucleus	43.71	34.76
Bra006521.1-P	Field mustard	nucleus	40.72	34.17
CDX88871	Canola	nucleus	42.52	33.49
CDX88650	Canola	nucleus	41.32	32.86
Bra006791.1-P	Field mustard	nucleus	41.32	32.86
CDY11839	Canola	nucleus	41.32	32.86
CDX70895	Canola	nucleus	40.72	32.69
Os12t0139500-01	Rice	nucleus	32.93	24.23
Os11t0143400-00	Rice	nucleus	32.93	16.37

Protein Annotations

MapMan:15.1.6.3	Gene3D:3.40.1340.10	Gene3D:3.90.940.20	EMBL:AK100492	UniProt:B7EQI7	ProteinID:BAS89806.1
InterPro:DNA_RNA_pol_RPB5_euk/virus	GO:GO:0001054	GO:GO:0001055	GO:GO:0001056	GO:GO:0003674	GO:GO:0003676
GO:GO:0003677	GO:GO:0003824	GO:GO:0003899	GO:GO:0005488	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005634	GO:GO:0005654	GO:GO:0005665	GO:GO:0005666	GO:GO:0005730
GO:GO:0005736	GO:GO:0006139	GO:GO:0006351	GO:GO:0006360	GO:GO:0006366	GO:GO:0006383
GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0016740	InterPro:IPR035913
InterPro:IPR036710	EnsemblPlantsGene:Os04g0487300	EnsemblPlants:Os04t0487300-01	PFAM:PD005155	PFAM:PF01191	PFAM:PF03871
PIRSF:PIRSF000747	PANTHER:PTHR10535	InterPro:RNA_pol_Rpb5_N	InterPro:RNA_pol_Rpb5_N_sf	InterPro:RNA_pol_subH/Rpb5_C	InterPro:RPB5-like
SUPFAM:SSF53036	SUPFAM:SSF55287	UniParc:UPI0000DD9141	:	:	:

Description

Similar to DNA-directed RNA polymerases II 24 kDa polypeptide. (Os04t0487300-01)

Coordinates

chr4:-:24352461..24355355

Molecular Weight (calculated)

19536.5 Da

IEP (calculated)

7.886

GRAVY (calculated)

-0.466

Length

167 amino acids

Sequence
(BLAST)

001: MASEEETSRL FRIRRTVMQM LRDRGYLVTE LDIDLPRGDF VARFGDPVDR DHLVFSRHKK DNGADQIYVF FPKDAKPGVK TIRSYVERMK QESVFNGILV
101: VQQALSAFAR SAVQEVSQKF HLEVFQEAEL LVNIKDHTLV PEHELLTPEQ KKTLLERYTV KETQVWK

Best Arabidopsis Sequence Match ( AT3G22320.1 )
(BLAST)

001: MLTEEELKRL YRIQKTLMQM LRDRGYFIAD SELTMTKQQF IRKHGDNMKR EDLVTLKAKR NDNSDQLYIF FPDEAKVGVK TMKMYTNRMK SENVFRAILV
101: VQQNLTPFAR TCISEISSKF HLEVFQEAEM LVNIKEHVLV PEHQVLTTEE KKTLLERYTV KETQLPRIQV TDPIARYFGL KRGQVVKIIR PSETAGRYVT
201: YRYVV

Arabidopsis Description

NRPB5ADNA-directed RNA polymerases II and IV subunit 5A [Source:UniProtKB/Swiss-Prot;Acc:O81098]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.