CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 4
plastid 1
mitochondrion 1
cytosol 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG99861
KRH46807

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G22320.1	KRG99861	AT3G24650.1	10743655
AT3G22320.1	KRH46807	AT3G24650.1	10743655

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH48354	Soybean	nucleus	99.03	99.03
VIT_11s0016g03530.t01	Wine grape	nucleus	83.5	83.5
Solyc05g051790.2.1	Tomato	nucleus	73.79	80.85
CDY24303	Canola	nucleus	80.1	80.1
CDY35542	Canola	nucleus	80.1	80.1
CDX92939	Canola	nucleus	80.1	80.1
Bra001864.1-P	Field mustard	nucleus	80.1	80.1
PGSC0003DMT400051454	Potato	nucleus	79.61	79.61
PGSC0003DMT400077392	Potato	nucleus	78.16	78.16
AT3G22320.1	Thale cress	nucleus	77.67	78.05
Bra031350.1-P	Field mustard	nucleus	77.18	77.18
CDY10635	Canola	nucleus	76.7	76.7
CDY37407	Canola	nucleus	76.21	76.21
Solyc03g025570.2.1	Tomato	cytosol	43.2	76.07
GSMUA_Achr2P00920_001	Banana	nucleus	71.84	72.55
GSMUA_AchrUn_...	Banana	nucleus	72.82	72.46
Zm00001d012557_P001	Maize	extracellular	68.45	67.14
Os01t0805800-01	Rice	nucleus	68.45	67.14
KXG33574	Sorghum	nucleus	68.45	67.14
HORVU3Hr1G078930.1	Barley	nucleus	66.5	65.24
TraesCS3D01G326800.2	Wheat	nucleus	66.5	65.24
TraesCS3B01G363400.1	Wheat	nucleus	66.5	65.24
TraesCS3A01G333200.2	Wheat	nucleus	66.5	65.24
PGSC0003DMT400069997	Potato	nucleus	65.05	64.73
TraesCS2A01G346300.1	Wheat	nucleus	63.11	63.41
TraesCS2D01G345000.2	Wheat	nucleus	63.11	63.41
Solyc05g053510.1.1	Tomato	nucleus	63.59	63.28
TraesCS2B01G364700.1	Wheat	nucleus, plastid	61.65	61.95
TraesCS2B01G364600.1	Wheat	nucleus, plastid	62.62	61.72
TraesCS2D01G344900.1	Wheat	nucleus	62.62	61.72
KXG26596	Sorghum	nucleus	61.65	61.65
Zm00001d003242_P001	Maize	mitochondrion	61.65	61.65
TraesCS2A01G346200.1	Wheat	nucleus	62.14	61.24
Os04t0487300-01	Rice	nucleus	47.57	58.68
CDX70895	Canola	nucleus	56.31	55.77
HORVU2Hr1G084270.1	Barley	nucleus	53.4	53.92
TraesCS2A01G346100.2	Wheat	nucleus	53.4	53.92
Bra006521.1-P	Field mustard	nucleus	51.94	53.77
TraesCS2D01G344800.1	Wheat	mitochondrion, nucleus	52.91	53.43
TraesCS2B01G364500.1	Wheat	nucleus	52.91	53.43
CDX88871	Canola	nucleus	54.85	53.3
HORVU1Hr1G048500.1	Barley	endoplasmic reticulum	11.65	48.98
AT3G16680.1	Thale cress	golgi	20.39	48.28
HORVU6Hr1G071230.1	Barley	cytosol, endoplasmic reticulum, extracellular, golgi, nucleus, plasma membrane, plastid, vacuole	23.3	48.0
HORVU2Hr1G084300.6	Barley	nucleus, plastid	62.14	46.38
HORVU7Hr1G020400.1	Barley	cytosol, mitochondrion, nucleus, plastid	24.76	45.54
HORVU1Hr1G052270.3	Barley	cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	24.27	44.64
HORVU4Hr1G028860.1	Barley	cytosol, endoplasmic reticulum, extracellular, golgi, nucleus, plasma membrane, plastid, vacuole	24.27	44.64
HORVU2Hr1G026650.1	Barley	cytosol, endoplasmic reticulum, extracellular, golgi, nucleus, plasma membrane, plastid, vacuole	23.79	43.75
HORVU6Hr1G011700.1	Barley	endoplasmic reticulum, nucleus	32.52	36.81
KRG95122	Soybean	nucleus	33.01	33.17
KRH62911	Soybean	nucleus	29.13	32.79
KRH66799	Soybean	nucleus	34.95	30.51
KRH30040	Soybean	nucleus	31.55	27.54

Protein Annotations

EntrezGene:100306098	MapMan:15.1.6.3	Gene3D:3.40.1340.10	Gene3D:3.90.940.20	EMBL:ACUP02005930	EMBL:BT090041
UniProt:C6SXZ4	InterPro:DNA_RNA_pol_RPB5_euk/virus	EnsemblPlantsGene:GLYMA_09G192900	GO:GO:0001054	GO:GO:0001055	GO:GO:0001056
GO:GO:0003674	GO:GO:0003676	GO:GO:0003677	GO:GO:0003824	GO:GO:0003899	GO:GO:0005488
GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634	GO:GO:0005654	GO:GO:0005665
GO:GO:0005666	GO:GO:0005730	GO:GO:0005736	GO:GO:0006139	GO:GO:0006351	GO:GO:0006360
GO:GO:0006366	GO:GO:0006383	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987
GO:GO:0016740	InterPro:IPR035913	InterPro:IPR036710	EnsemblPlants:KRH39324	ProteinID:KRH39324	ProteinID:KRH39324.1
HAMAP:MF_00025	PFAM:PD005155	PFAM:PF01191	PFAM:PF03871	PIRSF:PIRSF000747	ScanProsite:PS01110
PANTHER:PTHR10535	InterPro:RNA_pol_Rpb5_N	InterPro:RNA_pol_Rpb5_N_sf	InterPro:RNA_pol_subH/Rpb5_C	InterPro:RNA_pol_subH/Rpb5_CS	InterPro:RPB5-like
InterPro:RpoH/RPB5	SUPFAM:SSF53036	SUPFAM:SSF55287	UniParc:UPI0001B1558B	:	:

Description

hypothetical protein

Coordinates

chr9:+:41744148..41745938

Molecular Weight (calculated)

24244.4 Da

IEP (calculated)

9.692

GRAVY (calculated)

-0.518

Length

206 amino acids

Sequence
(BLAST)

001: MVLSEEEIGR LYRIRKTVMQ MLRDRNFLVG DFEINMSKHE FKSKYGEHMK REDLVINKSK KDNSGDQIYV FFPDEPKVGV KTMKTYTNRM NSENVYRAIL
101: VTQTNLTPFA RTCISEISSK FHLEVFQEAE LLVNIKEHEL VPEHQVLTDA EKKTLLERYT VKETQLPRIQ VTDPVARYYG LKRGQVVKII RPSETAGRYV
201: TYRFVV

Best Arabidopsis Sequence Match ( AT3G22320.1 )
(BLAST)

001: MLTEEELKRL YRIQKTLMQM LRDRGYFIAD SELTMTKQQF IRKHGDNMKR EDLVTLKAKR NDNSDQLYIF FPDEAKVGVK TMKMYTNRMK SENVFRAILV
101: VQQNLTPFAR TCISEISSKF HLEVFQEAEM LVNIKEHVLV PEHQVLTTEE KKTLLERYTV KETQLPRIQV TDPIARYFGL KRGQVVKIIR PSETAGRYVT
201: YRYVV

Arabidopsis Description

NRPB5ADNA-directed RNA polymerases II and IV subunit 5A [Source:UniProtKB/Swiss-Prot;Acc:O81098]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.