Factsheet for PGSC0003DMT400051454

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 4
plastid 1
mitochondrion 1
cytosol 1

Predictors

GFP

MS/MS

Papers

PPI

Inferred distinct locusB in Crop

locusB	locations
PGSC0003DMT400051746
PGSC0003DMT400051934

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G22320.1	PGSC0003DMT400051746	AT3G24650.1	10743655
AT3G22320.1	PGSC0003DMT400051934	AT3G24650.1	10743655

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
PGSC0003DMT400077392	Potato	nucleus	95.15	95.15
Solyc03g025570.2.1	Tomato	cytosol	50.49	88.89
VIT_11s0016g03530.t01	Wine grape	nucleus	82.52	82.52
KRH48354	Soybean	nucleus	80.1	80.1
Bra001864.1-P	Field mustard	nucleus	79.61	79.61
KRH39324	Soybean	nucleus	79.61	79.61
CDX92939	Canola	nucleus	79.61	79.61
CDY35542	Canola	nucleus	79.61	79.61
CDY24303	Canola	nucleus	79.61	79.61
AT3G22320.1	Thale cress	nucleus	78.64	79.02
Bra031350.1-P	Field mustard	nucleus	76.7	76.7
CDY10635	Canola	nucleus	76.21	76.21
CDY37407	Canola	nucleus	75.73	75.73
GSMUA_AchrUn_...	Banana	nucleus	73.3	72.95
GSMUA_Achr2P00920_001	Banana	nucleus	69.9	70.59
PGSC0003DMT400069997	Potato	nucleus	69.9	69.57
Zm00001d012557_P001	Maize	extracellular	66.5	65.24
Os01t0805800-01	Rice	nucleus	66.5	65.24
Zm00001d003242_P001	Maize	mitochondrion	65.05	65.05
KXG26596	Sorghum	nucleus	64.56	64.56
TraesCS2A01G346300.1	Wheat	nucleus	63.59	63.9
HORVU3Hr1G078930.1	Barley	nucleus	65.05	63.81
TraesCS3D01G326800.2	Wheat	nucleus	65.05	63.81
TraesCS3B01G363400.1	Wheat	nucleus	65.05	63.81
TraesCS3A01G333200.2	Wheat	nucleus	65.05	63.81
KXG33574	Sorghum	nucleus	65.05	63.81
TraesCS2D01G345000.2	Wheat	nucleus	63.11	63.41
TraesCS2B01G364600.1	Wheat	nucleus, plastid	64.08	63.16
TraesCS2D01G344900.1	Wheat	nucleus	63.59	62.68
TraesCS2A01G346200.1	Wheat	nucleus	63.59	62.68
TraesCS2B01G364700.1	Wheat	nucleus, plastid	62.14	62.44
Os04t0487300-01	Rice	nucleus	47.57	58.68
Bra006521.1-P	Field mustard	nucleus	53.4	55.28
CDX70895	Canola	nucleus	55.34	54.81
CDX88871	Canola	nucleus	56.31	54.72
HORVU2Hr1G084270.1	Barley	nucleus	53.4	53.92
TraesCS2A01G346100.2	Wheat	nucleus	51.94	52.45
TraesCS2B01G364500.1	Wheat	nucleus	51.94	52.45
TraesCS2D01G344800.1	Wheat	mitochondrion, nucleus	51.94	52.45
HORVU6Hr1G071230.1	Barley	cytosol, endoplasmic reticulum, extracellular, golgi, nucleus, plasma membrane, plastid, vacuole	23.3	48.0
HORVU1Hr1G048500.1	Barley	endoplasmic reticulum	11.16	46.94
AT3G16680.1	Thale cress	golgi	19.42	45.98
HORVU2Hr1G084300.6	Barley	nucleus, plastid	61.16	45.65
PGSC0003DMT400057891	Potato	nucleus	21.84	45.45
HORVU7Hr1G020400.1	Barley	cytosol, mitochondrion, nucleus, plastid	23.79	43.75
HORVU1Hr1G052270.3	Barley	cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	23.79	43.75
HORVU4Hr1G028860.1	Barley	cytosol, endoplasmic reticulum, extracellular, golgi, nucleus, plasma membrane, plastid, vacuole	23.3	42.86
HORVU2Hr1G026650.1	Barley	cytosol, endoplasmic reticulum, extracellular, golgi, nucleus, plasma membrane, plastid, vacuole	22.82	41.96
HORVU6Hr1G011700.1	Barley	endoplasmic reticulum, nucleus	32.04	36.26
PGSC0003DMT400027609	Potato	cytosol	36.41	33.04

Protein Annotations

EntrezGene:102590791	EntrezGene:102597013	MapMan:15.1.6.3	Gene3D:3.40.1340.10	Gene3D:3.90.940.20	ncoils:Coil
InterPro:DNA_RNA_pol_RPB5_euk/virus	GO:GO:0003674	GO:GO:0003676	GO:GO:0003677	GO:GO:0003824	GO:GO:0003899
GO:GO:0005488	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634	GO:GO:0006139
GO:GO:0006351	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0016740
InterPro:IPR035913	InterPro:IPR036710	UniProt:M1BRS4	HAMAP:MF_00025	PFAM:PD005155	PFAM:PF01191
PFAM:PF03871	EnsemblPlantsGene:PGSC0003DMG400019984	PGSC:PGSC0003DMG400019984	EnsemblPlants:PGSC0003DMT400051454	PIRSF:PIRSF000747	PANTHER:PTHR10535
InterPro:RNA_pol_Rpb5_N	InterPro:RNA_pol_Rpb5_N_sf	InterPro:RNA_pol_subH/Rpb5_C	InterPro:RPB5-like	InterPro:RpoH/RPB5	SUPFAM:SSF53036
SUPFAM:SSF55287	UniParc:UPI0002962940	RefSeq:XP_006353289.1	RefSeq:XP_015166765.1	:	:

Description

DNA-directed RNA polymerase II [Source:PGSC_GENE;Acc:PGSC0003DMG400019984]

Coordinates

chr3:+:43242102..43247855

Molecular Weight (calculated)

24413.9 Da

IEP (calculated)

9.766

GRAVY (calculated)

-0.466

Length

206 amino acids

Sequence
(BLAST)

001: MTLSEEELSR LFRVRKTLME MLSDRGYLVG DFEINMSKYE FLQKYGENMK REDLVLQKAK RNNSSDQIYV FFPEEPKVGV KTMKTYTERM KKEDVFQAIL
101: IVQQNLTPFA RTCISEISIK FHLEVFQEAE MLVNIKNHVL IPKHQTLTPE EKKTLLERYT VKETQLPRIQ ITDPIARYYG LKRGQVVKII RPSETAGRYV
201: TYRYVV

Best Arabidopsis Sequence Match ( AT3G22320.1 )
(BLAST)

001: MLTEEELKRL YRIQKTLMQM LRDRGYFIAD SELTMTKQQF IRKHGDNMKR EDLVTLKAKR NDNSDQLYIF FPDEAKVGVK TMKMYTNRMK SENVFRAILV
101: VQQNLTPFAR TCISEISSKF HLEVFQEAEM LVNIKEHVLV PEHQVLTTEE KKTLLERYTV KETQLPRIQV TDPIARYFGL KRGQVVKIIR PSETAGRYVT
201: YRYVV

Arabidopsis Description

NRPB5ADNA-directed RNA polymerases II and IV subunit 5A [Source:UniProtKB/Swiss-Prot;Acc:O81098]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.