Factsheet for PGSC0003DMT400069997

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 4
plastid 1
cytosol 2
mitochondrion 1

Predictors

GFP

MS/MS

Papers

PPI

Inferred distinct locusB in Crop

locusB	locations
PGSC0003DMT400051746
PGSC0003DMT400051934

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G22320.1	PGSC0003DMT400051746	AT3G24650.1	10743655
AT3G22320.1	PGSC0003DMT400051934	AT3G24650.1	10743655

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Solyc05g053510.1.1	Tomato	nucleus	88.89	88.89
PGSC0003DMT400051454	Potato	nucleus	69.57	69.9
PGSC0003DMT400077392	Potato	nucleus	69.08	69.42
KRH48354	Soybean	nucleus	65.22	65.53
KRH39324	Soybean	nucleus	64.73	65.05
VIT_11s0016g03530.t01	Wine grape	nucleus	63.77	64.08
AT3G22320.1	Thale cress	nucleus	62.8	63.41
CDY24303	Canola	nucleus	62.8	63.11
CDY35542	Canola	nucleus	62.8	63.11
Bra001864.1-P	Field mustard	nucleus	62.8	63.11
CDX92939	Canola	nucleus	62.8	63.11
GSMUA_AchrUn_...	Banana	nucleus	61.84	61.84
GSMUA_Achr2P00920_001	Banana	nucleus	59.42	60.29
Bra031350.1-P	Field mustard	nucleus	59.9	60.19
CDY10635	Canola	nucleus	59.42	59.71
CDY37407	Canola	nucleus	58.94	59.22
KXG33574	Sorghum	nucleus	58.45	57.62
Os01t0805800-01	Rice	nucleus	57.97	57.14
HORVU3Hr1G078930.1	Barley	nucleus	57.49	56.67
TraesCS3D01G326800.2	Wheat	nucleus	57.49	56.67
TraesCS3B01G363400.1	Wheat	nucleus	57.49	56.67
TraesCS3A01G333200.2	Wheat	nucleus	57.49	56.67
KXG26596	Sorghum	nucleus	56.04	56.31
Zm00001d012557_P001	Maize	extracellular	57.0	56.19
Zm00001d003242_P001	Maize	mitochondrion	54.59	54.85
TraesCS2D01G345000.2	Wheat	nucleus	52.66	53.17
TraesCS2A01G346300.1	Wheat	nucleus	52.66	53.17
TraesCS2D01G344900.1	Wheat	nucleus	53.14	52.63
TraesCS2B01G364600.1	Wheat	nucleus, plastid	53.14	52.63
TraesCS2B01G364700.1	Wheat	nucleus, plastid	51.69	52.2
TraesCS2A01G346200.1	Wheat	nucleus	52.66	52.15
Bra006521.1-P	Field mustard	nucleus	47.83	49.75
Os04t0487300-01	Rice	nucleus	39.61	49.1
CDX70895	Canola	nucleus	48.79	48.56
CDX88871	Canola	nucleus	49.28	48.11
TraesCS2A01G346100.2	Wheat	nucleus	45.89	46.57
HORVU2Hr1G084270.1	Barley	nucleus	44.93	45.59
TraesCS2B01G364500.1	Wheat	nucleus	44.44	45.1
TraesCS2D01G344800.1	Wheat	mitochondrion, nucleus	44.44	45.1
HORVU1Hr1G048500.1	Barley	endoplasmic reticulum	10.63	44.9
HORVU6Hr1G071230.1	Barley	cytosol, endoplasmic reticulum, extracellular, golgi, nucleus, plasma membrane, plastid, vacuole	20.77	43.0
AT3G16680.1	Thale cress	golgi	17.39	41.38
HORVU1Hr1G052270.3	Barley	cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	21.74	40.18
HORVU2Hr1G026650.1	Barley	cytosol, endoplasmic reticulum, extracellular, golgi, nucleus, plasma membrane, plastid, vacuole	21.74	40.18
HORVU7Hr1G020400.1	Barley	cytosol, mitochondrion, nucleus, plastid	21.74	40.18
HORVU4Hr1G028860.1	Barley	cytosol, endoplasmic reticulum, extracellular, golgi, nucleus, plasma membrane, plastid, vacuole	21.74	40.18
PGSC0003DMT400057891	Potato	nucleus	18.84	39.39
HORVU2Hr1G084300.6	Barley	nucleus, plastid	52.17	39.13
HORVU6Hr1G011700.1	Barley	endoplasmic reticulum, nucleus	27.54	31.32
PGSC0003DMT400027609	Potato	cytosol	33.33	30.4

Protein Annotations

EntrezGene:102590023	MapMan:15.1.6.3	Gene3D:3.40.1340.10	Gene3D:3.90.940.20	InterPro:DNA_RNA_pol_RPB5_euk/virus	GO:GO:0003674
GO:GO:0003676	GO:GO:0003677	GO:GO:0003824	GO:GO:0003899	GO:GO:0005488	GO:GO:0005575
GO:GO:0005622	GO:GO:0005623	GO:GO:0005634	GO:GO:0006139	GO:GO:0006351	GO:GO:0008150
GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0016740	InterPro:IPR035913	InterPro:IPR036710
UniProt:M1CLJ4	HAMAP:MF_00025	PFAM:PD005155	PFAM:PF01191	PFAM:PF03871	EnsemblPlantsGene:PGSC0003DMG400027218
PGSC:PGSC0003DMG400027218	EnsemblPlants:PGSC0003DMT400069997	PIRSF:PIRSF000747	PANTHER:PTHR10535	InterPro:RNA_pol_Rpb5_N	InterPro:RNA_pol_Rpb5_N_sf
InterPro:RNA_pol_subH/Rpb5_C	InterPro:RPB5-like	InterPro:RpoH/RPB5	SUPFAM:SSF53036	SUPFAM:SSF55287	UniParc:UPI000294F7BA
RefSeq:XP_006355758.1	:	:	:	:	:

Description

DNA-directed RNA polymerases II 24 kDa polypeptide [Source:PGSC_GENE;Acc:PGSC0003DMG400027218]

Coordinates

chr5:-:48779009..48780523

Molecular Weight (calculated)

24464.9 Da

IEP (calculated)

9.751

GRAVY (calculated)

-0.523

Length

207 amino acids

Sequence
(BLAST)

001: MSTLSEDELT RLYKVRKTMM EMLNDRGYLV EEFEIKMSKQ EFLQKYGVHM KRQDLEILKA KRNNDEKKIY VFFPEGAKVG VKEMRPYAER LRQEEISQAI
101: FVVQQKLTPK SLQFISEVST MLHLEVFQEA ELVVNVKDHA IVPEHQPLAN AEKKSLLERY TVKESQLPRI QFNDPIARYY GLKRGQIVKI IRPSETAGRY
201: VTYRYVV

Best Arabidopsis Sequence Match ( AT3G22320.1 )
(BLAST)

001: MLTEEELKRL YRIQKTLMQM LRDRGYFIAD SELTMTKQQF IRKHGDNMKR EDLVTLKAKR NDNSDQLYIF FPDEAKVGVK TMKMYTNRMK SENVFRAILV
101: VQQNLTPFAR TCISEISSKF HLEVFQEAEM LVNIKEHVLV PEHQVLTTEE KKTLLERYTV KETQLPRIQV TDPIARYFGL KRGQVVKIIR PSETAGRYVT
201: YRYVV

Arabidopsis Description

NRPB5ADNA-directed RNA polymerases II and IV subunit 5A [Source:UniProtKB/Swiss-Prot;Acc:O81098]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.