Factsheet for Bra033657.1-P

Subcellular Localization

min:

: max

Winner_takes_all: extracellular

Predictor Summary:

nucleus 1
vacuole 6
extracellular 6
endoplasmic reticulum 5
plasma membrane 4
golgi 4

Predictors

GFP

MS/MS

Papers

Winner Takes All:	extracellular
Any Predictor:	nucleus, secretory
BaCelLo:	nucleus
EpiLoc:	vacuole
iPSORT:	secretory
MultiLoc:	extracellular
Plant-mPloc:	vacuole
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	extracellular
YLoc:	endoplasmic reticulum

PPI

Inferred distinct locusB in Crop

locusB	locations
Bra012293.1-P
Bra016405.1-P
Bra017948.1-P

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G43060.1	Bra012293.1-P	AT1G21750.1	18676877
AT5G43060.1	Bra016405.1-P	AT1G21750.1	18676877
AT5G43060.1	Bra017948.1-P	AT1G21750.1	18676877

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY22370	Canola	extracellular	99.78	99.78
CDX71540	Canola	extracellular	99.13	99.13
AT5G43060.1	Thale cress	endoplasmic reticulum	89.35	88.77
Bra040749.1-P	Field mustard	endoplasmic reticulum	79.35	79.87
Bra014057.1-P	Field mustard	endoplasmic reticulum	76.96	77.12
VIT_18s0001g09990.t01	Wine grape	endoplasmic reticulum	71.96	70.88
GSMUA_Achr3P01000_001	Banana	endoplasmic reticulum	68.7	67.38
KXG27269	Sorghum	endoplasmic reticulum	67.17	66.59
KRH05634	Soybean	endoplasmic reticulum	68.7	66.39
PGSC0003DMT400020654	Potato	cytosol	63.7	66.14
GSMUA_Achr4P06140_001	Banana	endoplasmic reticulum	67.39	66.1
GSMUA_Achr10P...	Banana	endoplasmic reticulum	67.17	65.88
GSMUA_Achr4P12910_001	Banana	endoplasmic reticulum	67.17	65.33
Zm00001d002065_P006	Maize	plasma membrane	66.3	65.17
Zm00001d026458_P002	Maize	endoplasmic reticulum	67.61	64.79
GSMUA_Achr1P15920_001	Banana	endoplasmic reticulum	65.87	64.47
TraesCS2A01G512100.1	Wheat	extracellular	64.35	64.21
TraesCS2B01G540100.2	Wheat	golgi	64.57	64.15
TraesCS2D01G513600.1	Wheat	endoplasmic reticulum, golgi, vacuole	64.35	63.93
KRH15406	Soybean	nucleus	68.91	63.91
Solyc04g078540.2.1	Tomato	endoplasmic reticulum	64.78	63.14
Solyc12g088670.1.1	Tomato	endoplasmic reticulum	63.91	63.09
KRH61341	Soybean	endoplasmic reticulum, extracellular, vacuole	49.78	62.4
GSMUA_Achr8P03350_001	Banana	endoplasmic reticulum, extracellular, vacuole	44.78	61.86
Bra022418.1-P	Field mustard	endoplasmic reticulum	60.43	61.78
HORVU2Hr1G113460.2	Barley	plasma membrane	65.43	61.55
Bra013705.1-P	Field mustard	extracellular	42.61	59.76
Bra011746.1-P	Field mustard	vacuole	48.48	59.63
Bra010592.1-P	Field mustard	extracellular, golgi, vacuole	48.04	59.09
Bra038206.1-P	Field mustard	extracellular, plasma membrane, vacuole	57.39	58.67
GSMUA_Achr1P09800_001	Banana	endoplasmic reticulum	59.57	57.93
GSMUA_AchrUn_...	Banana	endoplasmic reticulum, extracellular, golgi	41.09	55.1
Bra038208.1-P	Field mustard	endoplasmic reticulum	52.39	54.65
Bra033148.1-P	Field mustard	extracellular	43.04	53.37
Bra033146.1-P	Field mustard	vacuole	43.04	53.08
Bra012252.1-P	Field mustard	endoplasmic reticulum	40.22	52.56
Bra024736.1-P	Field mustard	endoplasmic reticulum, extracellular	42.39	52.42
Bra016447.1-P	Field mustard	endoplasmic reticulum, golgi, vacuole	40.65	52.23
Bra011608.1-P	Field mustard	endoplasmic reticulum	40.22	51.97
Bra018067.1-P	Field mustard	endoplasmic reticulum	39.78	50.97
Bra000615.1-P	Field mustard	endoplasmic reticulum	40.0	50.97
Bra019982.1-P	Field mustard	extracellular, golgi	46.74	48.53
Bra018066.1-P	Field mustard	endoplasmic reticulum	37.83	47.28
Bra025046.1-P	Field mustard	endoplasmic reticulum, extracellular	35.22	47.09
Bra021998.1-P	Field mustard	endoplasmic reticulum	35.0	46.53

Protein Annotations

Gene3D:3.10.20.500	Gene3D:3.90.70.10	MapMan:50.3.4	EnsemblPlantsGene:Bra033657	EnsemblPlants:Bra033657.1	EnsemblPlants:Bra033657.1-P
MEROPS:C01.A12	GO:GO:0003674	GO:GO:0003824	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005737	GO:GO:0005773	GO:GO:0005783	GO:GO:0005829	GO:GO:0006508	GO:GO:0006950
GO:GO:0008150	GO:GO:0008152	GO:GO:0008233	GO:GO:0008234	GO:GO:0009628	GO:GO:0009651
GO:GO:0016787	GO:GO:0019538	InterPro:Granulin	InterPro:Granulin_sf	InterPro:IPR037277	UniProt:M4EXW8
PFAM:PF00112	PFAM:PF00396	PFAM:PF08246	PRINTS:PR00705	ScanProsite:PS00139	ScanProsite:PS00639
ScanProsite:PS00640	PANTHER:PTHR12411	PANTHER:PTHR12411:SF523	InterPro:Papain_like_cys_pep_sf	InterPro:Pept_asp_AS	InterPro:Pept_cys_AS
InterPro:Pept_his_AS	InterPro:Peptidase_C1A	InterPro:Peptidase_C1A_C	InterPro:Prot_inhib_I29	SMART:SM00277	SMART:SM00645
SMART:SM00848	SUPFAM:SSF54001	SUPFAM:SSF57277	SignalP:SignalP-noTM	UniParc:UPI000253F80F	SEG:seg

Description

AT5G43060 (E=5e-224) | cysteine proteinase, putative / thiol protease, putative

Coordinates

chrA06:+:25777294..25779371

Molecular Weight (calculated)

50566.6 Da

IEP (calculated)

5.747

GRAVY (calculated)

-0.472

Length

460 amino acids

Sequence
(BLAST)

001: MGSVKVTILL LAMMIGVSYA ADMSIISYDE KHHITADNER SDAEVARIYE AWMEKHGKKA QSNGLVGEEK DQRFEIFKDN LRFIDEHNNK NLSYKLGLTR
101: FADLTNEEYR SIYLGAKPKK RVLKTSDRYQ PRVGDAIPDS VDWRKEGAVA AVKDQGSCGS CWAFSTIGAV EGINKIVTGD LISLSEQELV DCDTSYNQGC
201: NGGLMDYAFE FIIKNGGIDT EEDYPYKAAD GRCDQTRKNA KVVTIDAYED VPENNEAALK KALANQPISV AIEAGGRAFQ LYSSGVFDGI CGTELDHGVV
301: AVGYGTENGK DYWIVRNSWG GSWGESGYIK MARNIAEPTG KCGIAMEASY PIKKGQNPPN PGPSPPSPIK PPTQCDKYYS CPEGNTCCCL FKYGKYCFGW
401: GCCPLEAATC CDDNTSCCPH EYPVCNGDTC LMSKNSPFSV KALKRTPAKP FWAHSRKNMA

Best Arabidopsis Sequence Match ( AT5G43060.1 )
(BLAST)

001: MGFLKLSPMI LLLAMIGVSY AMDMSIISYD ENHHITTETS RSDSEVERIY EAWMVEHGKK KMNQNGLGAE KDQRFEIFKD NLRFIDEHNT KNLSYKLGLT
101: RFADLTNEEY RSMYLGAKPT KRVLKTSDRY QARVGDALPD SVDWRKEGAV ADVKDQGSCG SCWAFSTIGA VEGINKIVTG DLISLSEQEL VDCDTSYNQG
201: CNGGLMDYAF EFIIKNGGID TEADYPYKAA DGRCDQNRKN AKVVTIDSYE DVPENSEASL KKALAHQPIS VAIEAGGRAF QLYSSGVFDG LCGTELDHGV
301: VAVGYGTENG KDYWIVRNSW GNRWGESGYI KMARNIEAPT GKCGIAMEAS YPIKKGQNPP NPGPSPPSPI KPPTTCDKYF SCPESNTCCC LYKYGKYCFG
401: WGCCPLEAAT CCDDNSSCCP HEYPVCDVNR GTCLMSKNSP FSVKALKRTP AIPFWAKSRK HIA

Arabidopsis Description

RD21BProbable cysteine protease RD21B [Source:UniProtKB/Swiss-Prot;Acc:Q9FMH8]
SUBAcon: [endoplasmic reticulum]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.