CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, nucleus, cytosol

Predictor Summary:

nucleus 2
cytosol 3
plastid 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG99633
KRH06147
KRH46640
KRH47571

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G13730.1	KRH06147	AT3G13990.1	21798944
AT1G13730.1	KRH47571	AT3G13990.1	21798944
AT1G13730.1	KRG99633	AT5G48650.1	21798944
AT1G13730.1	KRH46640	AT5G48650.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH50515	Soybean	cytosol, endoplasmic reticulum, nucleus	81.94	92.48
KRH45051	Soybean	cytosol	44.52	44.81
KRH03143	Soybean	cytosol, nucleus	43.23	43.7
KRH01288	Soybean	cytosol	44.3	43.28
KRH56802	Soybean	cytosol, nucleus	42.8	43.07
KRH46640	Soybean	cytosol	40.0	40.26
KRG99633	Soybean	cytosol	40.43	39.5
CDY36405	Canola	cytosol	33.98	39.01
CDX98124	Canola	cytosol	33.33	38.65
Bra019707.1-P	Field mustard	cytosol	33.12	38.31
AT1G13730.1	Thale cress	cytosol	34.19	37.15
KRH57274	Soybean	cytosol, mitochondrion	16.77	36.28
CDY02983	Canola	cytosol	31.61	36.03
CDY51206	Canola	cytosol	31.61	35.85
CDY36616	Canola	cytosol	30.75	35.14
Bra025122.1-P	Field mustard	cytosol	29.46	34.25
AT2G03640.4	Thale cress	nucleus	32.47	33.33
CDY50308	Canola	cytosol	29.89	33.25
CDY54146	Canola	cytosol	30.54	32.35
CDY40904	Canola	nucleus	29.89	32.25
AT1G69250.1	Thale cress	cytosol	28.39	30.91
KRH67452	Soybean	cytosol	29.89	30.75
KRG95733	Soybean	cytosol	28.82	29.78
CDY07356	Canola	cytosol	25.16	29.62
Bra004006.1-P	Field mustard	cytosol	25.16	29.62
CDY04270	Canola	cytosol	26.45	29.5
CDY04271	Canola	cytosol	26.88	29.07
CDX95953	Canola	cytosol	29.25	29.06
Bra004367.1-P	Field mustard	cytosol	29.25	28.69
Bra038318.1-P	Field mustard	cytosol	29.89	27.2
Solyc05g012240.1.1	Tomato	cytosol	22.58	25.55
KRG92252	Soybean	cytosol	25.59	25.32
KRH33563	Soybean	cytosol	25.59	25.27
KRG90305	Soybean	cytosol	25.59	25.11
Bra004005.1-P	Field mustard	cytosol	26.67	23.71
CDY07355	Canola	cytosol	26.67	23.71
AT3G55540.1	Thale cress	cytosol	16.56	23.05
PGSC0003DMT400072921	Potato	cytosol	23.01	16.93
Solyc05g012230.2.1	Tomato	extracellular	8.17	9.25

Protein Annotations

EntrezGene:100805610	MapMan:16.9.1	Gene3D:3.10.450.50	Gene3D:3.30.70.330	EMBL:ACUP02012211	EnsemblPlantsGene:GLYMA_20G025500
GO:GO:0003674	GO:GO:0003676	GO:GO:0003723	GO:GO:0003729	GO:GO:0005488	GO:GO:0005575
GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005829	GO:GO:0006810	GO:GO:0008150
GO:GO:0030529	UniProt:I1NDI0	InterPro:IPR000504	InterPro:IPR012677	InterPro:IPR018222	EnsemblPlants:KRG89479
ProteinID:KRG89479	ProteinID:KRG89479.1	InterPro:NTF2	InterPro:NTF2-like_dom_sf	InterPro:Nuclear_transport_factor_2_euk	InterPro:Nucleotide-bd_a/b_plait_sf
PFAM:PF00076	PFAM:PF02136	PFscan:PS50102	PFscan:PS50177	PANTHER:PTHR10693	PANTHER:PTHR10693:SF41
InterPro:RBD_domain_sf	InterPro:RRM_dom	SMART:SM00360	SUPFAM:SSF54427	SUPFAM:SSF54928	UniParc:UPI000233D96C
SEG:seg	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr20:+:2788006..2792610

Molecular Weight (calculated)

50807.8 Da

IEP (calculated)

5.899

GRAVY (calculated)

-0.658

Length

465 amino acids

Sequence
(BLAST)

001: MAASEESSTT QMIGNAFVQQ YYSILHQEPD QVHRFYQESS ILSRPEEDGT MTMVTTTLEI NKKILSLDYT SFRVEILSAD AQPSFKDGVI VVVTGCLTGS
101: DNLKRKFTQS FFLAPQDKGY FVLNDVFRYV DEYKSVDIES VPANDAATAD ESAPTDAFVP EPEVIHVAED VPPSQTAVVD ADISVSKEVS QPLENGNVSV
201: TEKVVPVNHV KESSHQEHSH YHAEKAASNN ALEDTPKKSF ASIVNALKEN AAPFHVRVSP VKLVEQPRVS SIPAPEAPAP SIESPPEKNN ENGGKAYAIF
301: VANLPMNATV EQLERAFKKF GPIKQDGIQV RSNKQQQSCF GFVEFESATS MQSALEASPP VTLDGRRLSI EERRANNDRG RYSSGRGGYR NDRNDNFRGR
401: GNFSGGRGGG GYGNRNDSFE KRSEFSGRPR GGNNNGGGRS NGEAVPRSYQ NGGGAKVTRQ SVKVQ

Best Arabidopsis Sequence Match ( AT2G03640.2 )
(BLAST)

001: MTPESNAPSV DPQFVGNGFV QEYYNHLYDS TSEVHKFYLE DSMISRPGLD GEIVTIKSLK GINDQIMSID YKSSRIEILT ADSQSTLKNG VVTLVTGLVI
101: GNDGGRRKFS QSFFLVSRNG SYFVLNDTFR YVSDEFVEPE ATKEVEESQS TNAITAEPAN ESVEAVIVPT EAKTTVTKPA SAIPNGHAKV PEEKVVNENS
201: SLPKAAEAKL QEEVPKKSFA LIVQSLAQSA GTLQVKASPV KRKPVEKPVA APERKAPSPI RKQASAESIK PQAQGSSIFV ANLPMDATIE QLYETFKSFG
301: AIRKDGIQVR SYPEKKNCIG FVAFENGEAV KNVFQAHRES PIRIGNRRAS IEEKRGGNNQ NGNRVSTRNN SGYKNEDGFR RDGYKPRGSG VNGGRGYGRR
401: NSESNGDGKA YQNNGHGNTE AKN

Arabidopsis Description

Nuclear transport factor 2 (NTF2) family protein with RNA binding (RRM-RBD-RNP motifs) domain-containing protein [Source:UniProtKB/TrEMBL;Acc:F4IT98]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.