CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

plastid 8
mitochondrion 3

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH14338
KRH16935
KRH73802

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G01090.1	KRH14338	AT5G50850.1	21798944
AT1G01090.1	KRH16935	AT5G50850.1	21798944
AT1G01090.1	KRH73802	AT5G50850.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH47800	Soybean	plastid	96.3	96.3
HORVU2Hr1G086290.1	Barley	extracellular, vacuole	21.53	90.29
CDY37427	Canola	cytosol, mitochondrion, plastid	31.94	89.61
CDY37428	Canola	cytosol	29.4	86.39
KRH68960	Soybean	plastid	79.86	82.54
KRG97240	Soybean	plastid	80.09	81.03
PGSC0003DMT400035288	Potato	plastid	80.56	79.63
CDY37473	Canola	plastid	77.78	79.43
Bra032619.1-P	Field mustard	plastid	78.01	79.29
CDY32445	Canola	plastid	78.01	79.29
Bra033286.1-P	Field mustard	plastid	78.01	79.11
CDX90009	Canola	plastid	78.01	79.11
VIT_15s0048g02580.t01	Wine grape	plastid	78.7	78.52
AT1G01090.1	Thale cress	plastid	77.78	78.5
CDY15202	Canola	plastid	77.55	78.09
Solyc12g009410.1.1	Tomato	plastid	78.94	77.5
PGSC0003DMT400007571	Potato	plastid	78.7	77.27
Solyc12g009400.1.1	Tomato	plastid	78.7	77.27
TraesCS2D01G010700.1	Wheat	golgi	73.84	75.95
TraesCS2B01G012500.1	Wheat	plastid	73.61	75.53
TraesCS2A01G010100.1	Wheat	plastid	73.38	75.3
Os04t0119400-01	Rice	plastid	73.61	74.82
Solyc07g042920.1.1	Tomato	cytosol, mitochondrion, plastid	49.07	74.39
Zm00001d004473_P001	Maize	plastid	73.61	74.3
EES10385	Sorghum	plastid	73.61	73.78
GSMUA_Achr11P...	Banana	plastid	72.45	72.79
HORVU2Hr1G001600.1	Barley	plastid	72.45	68.49
KRH74712	Soybean	mitochondrion	30.56	36.07
KRG99219	Soybean	mitochondrion	28.47	35.76
KRH69435	Soybean	mitochondrion	32.64	35.61
KRH45768	Soybean	mitochondrion	31.71	34.77
GSMUA_Achr5P18750_001	Banana	plastid	67.59	32.37
KRG95283	Soybean	cytosol, mitochondrion, plastid	12.27	32.32
KRG99480	Soybean	cytosol	12.04	25.62
KRH53908	Soybean	cytosol	6.94	25.0
KRH64579	Soybean	endoplasmic reticulum	22.22	20.08
KRH53343	Soybean	mitochondrion	22.22	19.71
KRH64030	Soybean	mitochondrion, plastid	21.3	19.41

Protein Annotations

KEGG:00010+1.2.4.1	KEGG:00020+1.2.4.1	KEGG:00620+1.2.4.1	EntrezGene:100811207	Gene3D:3.40.50.970	MapMan:5.1.2.2.1.1
EMBL:ACUP02009827	ncoils:Coil	InterPro:DH_E1	EnsemblPlantsGene:GLYMA_16G018300	GO:GO:0003674	GO:GO:0003824
GO:GO:0004739	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0006086	GO:GO:0008150
GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0016491	GO:GO:0016624	GO:GO:0043231
GO:GO:0055114	UniProt:I1MKC6	EnsemblPlants:KRH06359	ProteinID:KRH06359	ProteinID:KRH06359.1	PFAM:PF00676
PANTHER:PTHR11516	PANTHER:PTHR11516:SF25	InterPro:Pyrv_DH_E1_asu_subgrp-y	SUPFAM:SSF52518	InterPro:THDP-binding	TIGRFAMs:TIGR03182
UniParc:UPI000233AA87	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr16:-:1619151..1623559

Molecular Weight (calculated)

47901.1 Da

IEP (calculated)

7.021

GRAVY (calculated)

-0.371

Length

432 amino acids

Sequence
(BLAST)

001: MSFTATKFAP SPLPLNSTTP RSNDKPLSFS FDHSKPNPSS SFLGSTRKLL RFNALAKPHA HTRASSSPVA AVLLERTSNL LVTKEEGLEL YEDMILGRFF
101: EDKCAEMYYR GKMFGFVHLY NGQETVSTGF IKLLKKEDSV VSTYRDHVHA LSKGVPSREV MSELFGKATG CCRGQGGSMH MFSKEHNLLG GFAFIGEGIP
201: VATGAAFSSK YRREVLKQAD CDHVTLAFFG DGTCNNGQFY ECLNMAALWK LPIVFVVENN LWAIGMSHLR ATSDPQIWKK GPAFGMPGVH VDGMDVLQVR
301: EVAKEAVGRA RRGEGPTLVE CETYRFRGHS LADPDELRDP AEKEHYAGRD PITALKKYLF ENNLANEQEL KTIEKKIDEI LEDAVEFADK SPLPPRSQLL
401: ENVFADPKGF GIGPDGSYRC EDPKFTQGTA HV

Best Arabidopsis Sequence Match ( AT1G01090.1 )
(BLAST)

001: MATAFAPTKL TATVPLHGSH ENRLLLPIRL APPSSFLGST RSLSLRRLNH SNATRRSPVV SVQEVVKEKQ STNNTSLLIT KEEGLELYED MILGRSFEDM
101: CAQMYYRGKM FGFVHLYNGQ EAVSTGFIKL LTKSDSVVST YRDHVHALSK GVSARAVMSE LFGKVTGCCR GQGGSMHMFS KEHNMLGGFA FIGEGIPVAT
201: GAAFSSKYRR EVLKQDCDDV TVAFFGDGTC NNGQFFECLN MAALYKLPII FVVENNLWAI GMSHLRATSD PEIWKKGPAF GMPGVHVDGM DVLKVREVAK
301: EAVTRARRGE GPTLVECETY RFRGHSLADP DELRDAAEKA KYAARDPIAA LKKYLIENKL AKEAELKSIE KKIDELVEEA VEFADASPQP GRSQLLENVF
401: ADPKGFGIGP DGRYRCEDPK FTEGTAQV

Arabidopsis Description

PDH-E1 ALPHAPyruvate dehydrogenase E1 component subunit alpha [Source:UniProtKB/TrEMBL;Acc:A0A178W8A7]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.