CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, nucleus, cytosol

Predictor Summary:

nucleus 1
vacuole 2
cytosol 3
plasma membrane 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG91452
KRH09113
KRH35361
KRH38565

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G12310.1	KRG91452	AT5G62070.1	21798944
AT1G12310.1	KRH09113	AT5G62070.1	21798944
AT1G12310.1	KRH35361	AT5G62070.1	21798944
AT1G12310.1	KRH38565	AT5G62070.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH77406	Soybean	cytosol, endoplasmic reticulum, mitochondrion, nucleus	97.96	97.96
VIT_02s0241g00140.t01	Wine grape	cytosol	90.48	90.48
CDX98074	Canola	cytosol	87.76	87.16
CDY30070	Canola	cytosol	87.76	87.16
AT1G12310.1	Thale cress	cytosol	87.07	86.49
Bra019756.1-P	Field mustard	cytosol	86.39	85.81
Bra026976.1-P	Field mustard	cytosol	86.39	85.81
CDY21044	Canola	cytosol	86.39	85.81
CDY26556	Canola	cytosol	86.39	85.81
CDY31259	Canola	cytosol	86.39	85.81
CDY15991	Canola	cytosol	85.71	85.14
PGSC0003DMT400070433	Potato	cytosol	85.03	85.03
AT1G62820.1	Thale cress	cytosol	83.67	83.11
Solyc03g083520.2.1	Tomato	cytosol, nucleus	84.35	82.67
Solyc11g072270.1.1	Tomato	cytosol	85.03	80.13
Os08t0117400-01	Rice	plasma membrane	78.23	77.7
Zm00001d035095_P001	Maize	cytosol, extracellular, mitochondrion	77.55	77.03
TraesCS7A01G316100.1	Wheat	cytosol, unclear	77.55	77.03
TraesCS7B01G217200.1	Wheat	cytosol, plastid	76.87	76.35
GSMUA_Achr3P26750_001	Banana	cytosol, plasma membrane	76.87	76.35
TraesCS7D01G313100.1	Wheat	cytosol	76.19	75.68
KRH04023	Soybean	cytosol	75.51	75.51
OQU79799	Sorghum	cytosol	75.51	75.0
Zm00001d049665_P001	Maize	cytosol, extracellular, mitochondrion, plasma membrane	75.51	75.0
HORVU7Hr1G075240.1	Barley	cytosol, plastid	78.23	58.97
KRG94975	Soybean	nucleus	53.74	53.02
KRH18664	Soybean	cytosol	43.54	45.71
KRH69070	Soybean	cytosol	44.9	44.0
KRH34350	Soybean	cytosol	44.22	43.33
KRH31638	Soybean	cytosol	44.22	43.33
KRG92449	Soybean	cytosol	43.54	42.67
KRG96966	Soybean	cytosol	42.18	41.61
KRH68729	Soybean	cytosol	40.82	40.27
KRG89812	Soybean	cytosol, mitochondrion	44.9	40.0
KRH67274	Soybean	cytosol	39.46	39.19
KRG95596	Soybean	cytosol	39.46	39.19
KRG94637	Soybean	cytosol	29.25	37.72
KRH34082	Soybean	cytosol	37.41	36.91
KRG92667	Soybean	cytosol	37.41	36.91
KRH71363	Soybean	cytosol	34.01	33.56
KRH32074	Soybean	cytosol	33.33	32.89
KRH30413	Soybean	cytosol	31.97	29.56
KRH25249	Soybean	cytosol	31.97	28.83
KRH56721	Soybean	cytosol	29.25	26.71
KRH62961	Soybean	cytosol	28.57	26.25
KRH43884	Soybean	cytosol	23.13	25.95
KRH10567	Soybean	endoplasmic reticulum	29.25	25.29
KRH07628	Soybean	cytosol	25.85	23.75
KRH54782	Soybean	cytosol	25.17	23.12
KRG98826	Soybean	cytosol	23.13	21.66
KRG98823	Soybean	cytosol	21.77	20.38

Protein Annotations

Gene3D:1.10.238.10	MapMan:35.1	EMBL:ACUP02006738	InterPro:EF-hand-dom_pair	InterPro:EF_Hand_1_Ca_BS	InterPro:EF_hand_dom
EnsemblPlantsGene:GLYMA_11G030100	GO:GO:0003674	GO:GO:0005488	GO:GO:0005509	UniProt:I1LGL7	InterPro:IPR002048
EnsemblPlants:KRH28027	ProteinID:KRH28027	ProteinID:KRH28027.1	PFAM:PF13405	PFAM:PF13499	ScanProsite:PS00018
PFscan:PS50222	PANTHER:PTHR23050	PANTHER:PTHR23050:SF214	SMART:SM00054	SUPFAM:SSF47473	UniParc:UPI00023CDC13

Description

hypothetical protein

Coordinates

chr11:+:2176733..2180098

Molecular Weight (calculated)

16555.7 Da

IEP (calculated)

4.484

GRAVY (calculated)

-0.394

Length

147 amino acids

Sequence
(BLAST)

001: MGKDLSDEQV SSMKEAFSLF DTDGDGRIAP SELGILMRSL GGNPTQAQLK AIVAEENLTA PFDFPRFLDL MAKHMKPEPF DRQLRDAFKV LDKDSTGFVA
101: VSELRHILTN IGEKLEPSEF DEWIREVDVG SDGKIRYEDF ISRMVAK

Best Arabidopsis Sequence Match ( AT1G12310.1 )
(BLAST)

001: MGKDGLSDDQ VSSMKEAFML FDTDGDGKIA PSELGILMRS LGGNPTQAQL KSIIASENLS SPFDFNRFLD LMAKHLKTEP FDRQLRDAFK VLDKEGTGFV
101: AVADLRHILT SIGEKLEPNE FDEWIKEVDV GSDGKIRYED FIARMVAK

Arabidopsis Description

CML13Probable calcium-binding protein CML13 [Source:UniProtKB/Swiss-Prot;Acc:Q94AZ4]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.