Factsheet for PGSC0003DMT400066160

Subcellular Localization

min:

: max

Winner_takes_all: extracellular

Predictor Summary:

nucleus 3
cytosol 2
mitochondrion 1
plastid 1

Predictors

GFP

MS/MS

Papers

extracellular: 22313117

msms PMID: 22313117 doi

S Lim, K Chisholm, RH Coffin, RD Peters, KI Al-Mughrabi, G Wang-Pruski, DM Pinto

Department of Plant and Animal Sciences, Nova Scotia Agricultural College, Truro, Nova Scotia, Canada.

PPI

Inferred distinct locusB in Crop

locusB	locations
PGSC0003DMT400006927
PGSC0003DMT400008672
PGSC0003DMT400021644
PGSC0003DMT400026059
PGSC0003DMT400035614
PGSC0003DMT400043032
PGSC0003DMT400045025
PGSC0003DMT400047304
PGSC0003DMT400052548
PGSC0003DMT400060294
PGSC0003DMT400066160
PGSC0003DMT400066954
PGSC0003DMT400067201
PGSC0003DMT400069054
PGSC0003DMT400070431
PGSC0003DMT400079660

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G19590.1	PGSC0003DMT400008672	AT1G67210.1	20706207
AT3G19590.1	PGSC0003DMT400035614	AT2G06990.1	20706207
AT3G19590.1	PGSC0003DMT400052548	AT2G06990.1	20706207
AT3G19590.1	PGSC0003DMT400043032	AT2G22640.1	25262777
AT3G19590.1	PGSC0003DMT400067201	AT3G11830.1	20706207
AT3G19590.1	PGSC0003DMT400066160	AT3G19590.1	25262777
AT3G19590.1	PGSC0003DMT400066954	AT3G20050.1	20706207
AT3G19590.1	PGSC0003DMT400045025	AT3G25980.1	25262777
AT3G19590.1	PGSC0003DMT400069054	AT4G10110.1	20706207
AT3G19590.1	PGSC0003DMT400047304	AT4G28230.1	20706207
AT3G19590.1	PGSC0003DMT400006927	AT5G03740.1	20706207
AT3G19590.1	PGSC0003DMT400021644	AT5G03740.1	20706207
AT3G19590.1	PGSC0003DMT400026059	AT5G15980.1	20706207
AT3G19590.1	PGSC0003DMT400060294	AT5G26360.1	20706207
AT3G19590.1	PGSC0003DMT400070431	AT5G51600.1	20706207
AT3G19590.1	PGSC0003DMT400079660	AT5G51600.1	20706207

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Solyc01g107160.2.1	Tomato	nucleus	99.71	99.71
VIT_03s0091g00710.t01	Wine grape	cytosol	88.82	89.61
KRH35682	Soybean	nucleus	87.35	87.35
KRG91104	Soybean	nucleus	87.94	86.92
KRG91092	Soybean	nucleus	86.47	86.47
Bra038220.1-P	Field mustard	cytosol, plastid	83.53	83.53
CDX95434	Canola	cytosol, plastid	83.53	83.53
CDY42193	Canola	cytosol, plastid	83.53	83.53
Os03t0448600-01	Rice	cytosol	83.82	83.09
AT3G19590.1	Thale cress	cytosol	82.65	82.65
EER87819	Sorghum	cytosol	82.94	82.22
CDY59371	Canola	cytosol, plastid	81.76	82.01
Bra014226.1-P	Field mustard	cytosol, plastid	81.76	82.01
AT1G49910.1	Thale cress	cytosol	81.76	82.01
CDY03579	Canola	cytosol, plastid	81.76	81.76
TraesCS4A01G058600.1	Wheat	cytosol, plastid	82.94	81.74
TraesCS4D01G245500.2	Wheat	cytosol, plastid	82.65	81.45
TraesCS4B01G246100.1	Wheat	cytosol	68.24	75.57
HORVU4Hr1G067810.1	Barley	plastid	82.65	70.25
KRH35672	Soybean	cytosol	25.59	66.41
Os07t0588500-00	Rice	cytosol	70.59	65.93
TraesCS2A01G194000.1	Wheat	cytosol	64.12	63.01
TraesCS2D01G193800.1	Wheat	cytosol	63.24	62.87
TraesCS2B01G212800.1	Wheat	cytosol	63.53	62.25
PGSC0003DMT400072830	Potato	cytosol	35.0	36.62
GSMUA_Achr4P03390_001	Banana	extracellular	43.82	35.22

Protein Annotations

EntrezGene:102588228	MapMan:13.3.5.5.2	Gene3D:2.130.10.10	InterPro:G-protein_beta_WD-40_rep	GO:GO:0003674	GO:GO:0005488
GO:GO:0005515	InterPro:IPR001680	InterPro:IPR015943	InterPro:IPR017986	UniProt:M1CF39	PFAM:PF00400
EnsemblPlantsGene:PGSC0003DMG400025750	PGSC:PGSC0003DMG400025750	EnsemblPlants:PGSC0003DMT400066160	PRINTS:PR00320	PFscan:PS50082	PFscan:PS50294
PANTHER:PTHR10971	PANTHER:PTHR10971:SF24	SMART:SM00320	SUPFAM:SSF50978	UniParc:UPI0002962C34	InterPro:WD40/YVTN_repeat-like_dom_sf
InterPro:WD40_repeat	InterPro:WD40_repeat_dom	InterPro:WD40_repeat_dom_sf	RefSeq:XP_006346313.1	SEG:seg	:

Description

Mitotic checkpoint protein bub3 [Source:PGSC_GENE;Acc:PGSC0003DMG400025750]

Coordinates

chr1:+:83662966..83668182

Molecular Weight (calculated)

37765.1 Da

IEP (calculated)

8.196

GRAVY (calculated)

-0.536

Length

340 amino acids

Sequence
(BLAST)

001: MTAVSQSAGR ELENPPKDGI SNLRFSNHSD HLLVSSWDKT VRLYDASANA LRGEFMHGGA VLDCCFHDDS SGFSASADNT VRRLVFNSGR EDILGRHDAP
101: VRCIDYSYAT GQVITGSWDK TLKCWDPRGA SGQEHTLVGT YTQPERVYSL SLVGNRLVVA TAGRHVNVYD LRNMNQPEQR RESSLKYQTR CVRCYPNGTG
201: YALSSVEGRV AMEFFDLSES GQSKKYAFKC HRKSEAGRDI VYPVNAIAFH PIYGTFATGG CDGYVNVWDG NNKKRLYQYP KYPSSVAALS FSRDGKLLAV
301: ASSYTFEEGE KPHEPDAIYV RSVNEVEVKP KPKVLPNPTS

Best Arabidopsis Sequence Match ( AT3G19590.1 )
(BLAST)

001: MTTVTPSAGR ELSNPPSDGI SNLRFSNNSD HLLVSSWDKR VRLYDVSTNS LKGEFLHGGA VLDCCFHDDF SGFSVGADYK VRRIVFNVGK EDILGTHDKA
101: VRCVEYSYAA GQVITGSWDK TVKCWDPRGA SGPERTQVGT YLQPERVYSM SLVGHRLVVA TAGRHVNIYD LRNMSQPEQR RESSLKYQTR CVRCYPNGTG
201: YALSSVEGRV AMEFFDLSEA AQAKKYAFKC HRKSEAGRDI VYPVNSIAFH PIYGTFATGG CDGFVNIWDG NNKKRLYQYS KYPTSISALS FSRDGQLLAV
301: ASSYTFEEGE KSQEPEAIFV RSVNEIEVKP KPKVYPNPAA

Arabidopsis Description

BUB3.1BUB3.1 [Source:UniProtKB/TrEMBL;Acc:A0A178VA46]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.