CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

cytosol 1
extracellular 4
endoplasmic reticulum 4
vacuole 4
plasma membrane 7
golgi 5

Predictors

GFP

MS/MS

Papers

Winner Takes All:	endoplasmic reticulum
Any Predictor:	cytosol, secretory
BaCelLo:	cytosol
iPSORT:	secretory
MultiLoc:	golgi
Plant-mPloc:	plasma membrane
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plasma membrane
YLoc:	plasma membrane

endoplasmic reticulum: 28499913

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG93519
KRG96023
KRH18485
KRH19897
KRH21098
KRH32507
KRH32778
KRH59216
KRH67730
KRH72265

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G57090.1	KRH21098	AT2G34650.1	17889649
AT5G57090.1	KRH59216	AT2G34650.1	17889649
AT5G57090.1	KRG96023	AT2G36910.1	17237354
AT5G57090.1	KRH19897	AT2G36910.1	17237354
AT5G57090.1	KRH32507	AT2G36910.1	17237354
AT5G57090.1	KRH67730	AT2G36910.1	17237354
AT5G57090.1	KRG93519	AT3G28860.1	17237354
AT5G57090.1	KRH18485	AT3G28860.1	17237354
AT5G57090.1	KRH32778	AT5G06140.1	16936718
AT5G57090.1	KRH72265	AT5G06140.1	16936718

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH02749	Soybean	plasma membrane	96.88	97.65
VIT_11s0052g00440.t01	Wine grape	plasma membrane	80.06	81.59
GSMUA_Achr1P15720_001	Banana	plasma membrane	62.62	78.36
AT5G57090.1	Thale cress	golgi, plasma membrane, vacuole	78.82	78.21
PGSC0003DMT400048251	Potato	plasma membrane	76.64	77.97
Bra006834.1-P	Field mustard	plasma membrane	77.57	77.57
CDY11886	Canola	plasma membrane	74.77	77.54
Solyc07g006900.1.1	Tomato	plasma membrane	76.17	77.5
CDY20694	Canola	plasma membrane	75.7	76.54
Bra035648.1-P	Field mustard	plasma membrane	78.35	76.1
CDY69709	Canola	plasma membrane	71.65	75.91
CDX88606	Canola	plasma membrane	74.92	74.81
CDY38263	Canola	plasma membrane	64.8	71.97
Bra002763.1-P	Field mustard	plasma membrane	65.89	71.09
CDY53978	Canola	plasma membrane	65.73	70.81
KRH18110	Soybean	extracellular	13.71	69.84
Os06t0660200-01	Rice	plasma membrane	66.82	68.1
EER88769	Sorghum	plasma membrane	66.36	68.05
TraesCS7B01G398100.1	Wheat	golgi, plasma membrane, vacuole	66.2	67.25
TraesCS7A01G492400.1	Wheat	golgi, plasma membrane, vacuole	66.2	67.25
TraesCS7D01G478800.1	Wheat	golgi, plasma membrane, vacuole	66.2	67.14
HORVU7Hr1G110470.7	Barley	golgi, plasma membrane, vacuole	66.2	67.03
KRH37366	Soybean	plasma membrane	45.95	66.59
Zm00001d046893_P001	Maize	golgi, plasma membrane, vacuole	65.89	66.51
KRH41847	Soybean	golgi, plasma membrane	60.59	64.51
KRH66749	Soybean	plasma membrane	59.81	64.32
KRH48642	Soybean	plasma membrane	59.19	62.81
KRH12968	Soybean	plasma membrane	47.51	61.99
KRG95082	Soybean	plasma membrane	45.95	61.33
KRH38202	Soybean	plasma membrane	60.44	61.2
KRH49577	Soybean	plasma membrane	52.02	60.07
KRH37897	Soybean	plasma membrane	44.24	58.32
KRH50369	Soybean	plasma membrane	60.12	58.05
KRG89296	Soybean	plasma membrane	60.12	57.96
KRH39071	Soybean	plasma membrane	37.23	56.9
KRH15941	Soybean	plasma membrane	45.33	54.8
KRH01128	Soybean	plasma membrane	29.59	52.92
KRH40132	Soybean	plasma membrane	29.28	52.51
KRH58174	Soybean	endoplasmic reticulum, plasma membrane	28.35	50.28
KRH04363	Soybean	endoplasmic reticulum, plasma membrane	28.19	49.86
KRH12361	Soybean	endoplasmic reticulum, golgi	35.98	49.25
KRH18109	Soybean	plasma membrane	26.48	48.57
KRH00905	Soybean	golgi, plasma membrane, vacuole	25.08	43.63
KRH40330	Soybean	golgi, plasma membrane, vacuole	25.08	42.71

Protein Annotations

EntrezGene:100499638	MapMan:11.2.4.1.1	MapMan:24.2.5.2.1	EMBL:ACUP02008170	InterPro:Auxin_eff_plant	EnsemblPlantsGene:GLYMA_13G101900
GO:GO:0003674	GO:GO:0005215	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737
GO:GO:0005783	GO:GO:0005886	GO:GO:0006810	GO:GO:0007154	GO:GO:0007165	GO:GO:0008150
GO:GO:0009719	GO:GO:0009734	GO:GO:0009926	GO:GO:0009987	GO:GO:0010252	GO:GO:0010315
GO:GO:0010329	GO:GO:0016020	GO:GO:0016021	GO:GO:0055085	UniProt:I1LVK8	EMBL:KC344375
EnsemblPlants:KRH19119	ProteinID:KRH19119	ProteinID:KRH19119.1	InterPro:Mem_trans	PFAM:PF03547	InterPro:PIN2
PANTHER:PTHR31752	PANTHER:PTHR31752:SF4	TIGRFAMs:TIGR00946	TMHMM:TMhelix	UniParc:UPI00023D07BE	SEG:seg

Description

hypothetical protein

Coordinates

chr13:-:21723985..21727453

Molecular Weight (calculated)

69749.5 Da

IEP (calculated)

9.434

GRAVY (calculated)

0.120

Length

642 amino acids

Sequence
(BLAST)

001: MITGKDIYDV FAAIVPLYVA MILAYGSVRW WKIFTPDQCS GINRFVAVFA VPLLSFHFIS SNDPYAMNYH FIAADSLQKV VILGALFLWN TFTKHGSLDW
101: TITLFSLSTL PNTLVMGIPL LKAMYGDFSG SLMVQIVVLQ SVIWYTFMLF MFEYRGAKLL ITEQFPETAG SITSFRVDSD VVSLNGREPL QTDAEIGEDG
201: KLHVVVKRSA ASSMISSFNK SHLTSMTPRA SNLTGVEIYS VQSSREPTPR ASSFNQTDFY AMFASKAPSP KHGYTNSFQS NGGIGDVYSL QSSKGATPRT
301: SNFEEEMLKM HKKRGGRSMS GELFNGGLVS SNYPPPNPMF SGSTSAGGPK KKDSSGGGAA AAPNTNKELH MFVWSSSASP VSEGNLRHAV NRAASTDFGT
401: VDPSKAVPHE TVASKAVHEL IENMSPGRRG NGDQRELEMD EGAKFAISGS PYTTCQNQKK VDMEDGNNAN KNQQMPPASV MTRLILIMVW RKLIRNPNTY
501: SSLLGLTWSL ISFRWHIEMP TIVKGSISIL SDAGLGMAMF SLGLFMALQP KIIACGKSVA AFSMAVRFLT GPAVIAATSI GIGLRGVLLH VAIVQAALPQ
601: GIVPFVFAKE YNLHADILST AVIFGMLIAL PITILYYVLL GV

Best Arabidopsis Sequence Match ( AT5G57090.1 )
(BLAST)

001: MITGKDMYDV LAAMVPLYVA MILAYGSVRW WGIFTPDQCS GINRFVAVFA VPLLSFHFIS SNDPYAMNYH FLAADSLQKV VILAALFLWQ AFSRRGSLEW
101: MITLFSLSTL PNTLVMGIPL LRAMYGDFSG NLMVQIVVLQ SIIWYTLMLF LFEFRGAKLL ISEQFPETAG SITSFRVDSD VISLNGREPL QTDAEIGDDG
201: KLHVVVRRSS AASSMISSFN KSHGGGLNSS MITPRASNLT GVEIYSVQSS REPTPRASSF NQTDFYAMFN ASKAPSPRHG YTNSYGGAGA GPGGDVYSLQ
301: SSKGVTPRTS NFDEEVMKTA KKAGRGGRSM SGELYNNNSV PSYPPPNPMF TGSTSGASGV KKKESGGGGS GGGVGVGGQN KEMNMFVWSS SASPVSEANA
401: KNAMTRGSST DVSTDPKVSI PPHDNLATKA MQNLIENMSP GRKGHVEMDQ DGNNGGKSPY MGKKGSDVED GGPGPRKQQM PPASVMTRLI LIMVWRKLIR
501: NPNTYSSLFG LAWSLVSFKW NIKMPTIMSG SISILSDAGL GMAMFSLGLF MALQPKIIAC GKSVAGFAMA VRFLTGPAVI AATSIAIGIR GDLLHIAIVQ
601: AALPQGIVPF VFAKEYNVHP DILSTAVIFG MLVALPVTVL YYVLLGL

Arabidopsis Description

PIN2Auxin efflux carrier component 2 [Source:UniProtKB/Swiss-Prot;Acc:Q9LU77]
SUBAcon: [plasma membrane,golgi,vacuole]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.