CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

extracellular 8
endoplasmic reticulum 5
vacuole 5
plasma membrane 5
golgi 7

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	secretory
BaCelLo:	secretory
EpiLoc:	golgi
iPSORT:	secretory
MultiLoc:	extracellular
Plant-mPloc:	extracellular
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	extracellular
YLoc:	golgi

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH42483
KRH58580

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G36360.1	KRH42483	AT2G01950.1	19000166
AT4G36360.1	KRH58580	AT2G01950.1	19000166

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH69834	Soybean	extracellular, golgi	96.34	96.34
KRH76716	Soybean	nucleus	86.91	86.81
KRH28740	Soybean	nucleus	86.67	86.17
VIT_04s0023g02690.t01	Wine grape	extracellular, golgi	81.72	82.01
PGSC0003DMT400009096	Potato	endoplasmic reticulum, golgi, vacuole	78.18	78.46
Solyc02g084720.2.1	Tomato	endoplasmic reticulum, golgi, vacuole	78.07	78.34
AT4G36360.1	Thale cress	endoplasmic reticulum, golgi, vacuole	78.66	77.92
Bra011677.1-P	Field mustard	golgi, vacuole	77.59	76.87
CDX69219	Canola	vacuole	74.53	76.7
CDX75569	Canola	golgi, vacuole	74.53	76.42
CDY59693	Canola	vacuole	76.89	76.17
KRH15132	Soybean	mitochondrion, nucleus	62.97	72.95
KRH61101	Soybean	nucleus	62.97	72.55
KRH05876	Soybean	nucleus	62.62	72.54
GSMUA_Achr9P21610_001	Banana	extracellular, golgi	72.52	72.1
GSMUA_Achr4P06620_001	Banana	cytosol	54.72	72.05
GSMUA_Achr4P13580_001	Banana	golgi, vacuole	73.7	72.0
CDY59691	Canola	cytosol, nucleus, vacuole	29.83	71.88
EER92805	Sorghum	vacuole	70.52	71.02
CDY59725	Canola	cytosol	25.94	70.51
Os03t0165400-01	Rice	vacuole	69.81	70.39
TraesCS4A01G022500.2	Wheat	golgi, vacuole	69.93	70.26
TraesCS4B01G280900.1	Wheat	golgi, vacuole	69.81	70.06
TraesCS4D01G279400.4	Wheat	vacuole	69.93	69.93
HORVU4Hr1G074280.4	Barley	vacuole	69.22	69.3
Zm00001d048440_P003	Maize	vacuole	70.28	68.58
KRH51779	Soybean	vacuole	57.19	68.02
Bra017756.1-P	Field mustard	cytosol, nucleus, vacuole	24.17	67.43
Zm00001d027803_P002	Maize	golgi, plasma membrane, vacuole	70.4	65.03
KRH10007	Soybean	nucleus	64.15	64.76
KRH23324	Soybean	vacuole	63.8	64.33
KRH44153	Soybean	nucleus	63.44	63.82
KRH47126	Soybean	nucleus	63.56	63.79
KRH37385	Soybean	nucleus	52.36	61.58
KRH12385	Soybean	vacuole	52.12	61.3
KRH19154	Soybean	golgi, mitochondrion, plastid, vacuole	58.49	59.12
KRH02714	Soybean	golgi, vacuole	58.25	58.88
KRH22910	Soybean	nucleus	55.54	56.07
KRG89008	Soybean	nucleus	54.36	55.14
KRH26431	Soybean	golgi, vacuole	54.6	55.12
KRH29942	Soybean	extracellular, golgi, vacuole	54.13	54.77
KRH63980	Soybean	vacuole	51.65	51.96
KRH53957	Soybean	nucleus	50.94	51.12
KRH40964	Soybean	golgi, vacuole	41.86	50.43
KRH64633	Soybean	vacuole	40.92	49.86
KRH63978	Soybean	golgi, vacuole	41.75	49.37
KRH53267	Soybean	golgi, vacuole	40.57	49.35
KRH53958	Soybean	golgi, vacuole	41.51	49.09
KRH19351	Soybean	extracellular, golgi, vacuole	47.76	48.97
KRH38264	Soybean	endoplasmic reticulum, golgi, vacuole	47.52	48.44
KRH02579	Soybean	extracellular, golgi, vacuole	47.52	47.75
KRH42784	Soybean	nucleus	50.71	47.3
KRH70131	Soybean	vacuole	46.23	46.95
KRH07365	Soybean	extracellular, golgi, vacuole	45.28	46.49
KRH48710	Soybean	vacuole	45.52	45.63
KRH48708	Soybean	vacuole	45.52	45.63
KRH06832	Soybean	mitochondrion	38.21	43.2
KRH38266	Soybean	cytosol, peroxisome	29.48	42.73
KRH60700	Soybean	extracellular, golgi, vacuole	42.22	42.42
KRH29286	Soybean	extracellular, golgi, vacuole	41.75	42.04
KRH24309	Soybean	golgi, vacuole	41.39	41.64

Protein Annotations

KEGG:00052+3.2.1.23	KEGG:00511+3.2.1.23	KEGG:00531+3.2.1.23	KEGG:00600+3.2.1.23	KEGG:00604+3.2.1.23	EntrezGene:100780897
Gene3D:2.60.120.260	Gene3D:2.60.120.740	MapMan:21.3.2.2.2	Gene3D:3.20.20.80	EMBL:ACUP02010146	EnsemblPlantsGene:GLYMA_16G131800
GO:GO:0003674	GO:GO:0003824	GO:GO:0004553	GO:GO:0004565	GO:GO:0005488	GO:GO:0005575
GO:GO:0005618	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005773	GO:GO:0005975
GO:GO:0008150	GO:GO:0008152	GO:GO:0016787	GO:GO:0016798	GO:GO:0030246	GO:GO:0030312
InterPro:Galactose-bd-like_sf	InterPro:Gly_Hdrlase_35_cat	InterPro:Glyco_hydro_35_CS	InterPro:Glycoside_Hdrlase_35	InterPro:Glycoside_hydrolase_SF	UniProt:I1MN91
InterPro:IPR000922	InterPro:IPR008979	EnsemblPlants:KRH08131	ProteinID:KRH08131	ProteinID:KRH08131.1	InterPro:Lectin_gal-bd_dom
PFAM:PF01301	PFAM:PF02140	PRINTS:PR00742	ScanProsite:PS01182	PFscan:PS50228	PANTHER:PTHR23421
PANTHER:PTHR23421:SF79	MetaCyc:PWY-6807	SUPFAM:SSF49785	SUPFAM:SSF51445	SignalP:SignalP-noTM	TMHMM:TMhelix
UniParc:UPI000233F1C0	SEG:seg	:	:	:	:

Description

hypothetical protein

Coordinates

chr16:+:28739594..28745224

Molecular Weight (calculated)

94557.3 Da

IEP (calculated)

7.281

GRAVY (calculated)

-0.263

Length

848 amino acids

Sequence
(BLAST)

001: METTSFSKLL FLFFCFFASC LSSHASVTYD RKAILINGQR RILFSGSIHY PRSTPDMWED LILKAKEGGL DVVETYVFWN VHEPSPGNYN FEGRYDLVRF
101: VKTIQKAGLY AHLRIGPYVC AEWNFGGFPV WLKYVPGISF RTDNEPFKTA MQGFTEKIVG MMKSERLFES QGGPIILSQI ENEYGAQSKL QGDAGQNYVN
201: WAAKMAVEMG TGVPWVMCKE DDAPDPVINT CNGFYCDKFT PNRPYKPMIW TEAWSGWFTE FGGPIHKRPV QDLAFAVARF IIRGGSFVNY YMYHGGTNFG
301: RTAGGPFIAT SYDYDAPLDE YGLIRQPKYG HLKELHRAIK MCERALVSTD PIITSLGESQ QAHVYTTESG DCAAFLSNYD SKSSARVMFN NMHYNLPPWS
401: VSILPDCRNV VFNTAKVGVQ TSQMQMLPTN TQLFSWESFD EDVYSVDDSS AIMAPGLLEQ INVTKDASDY LWYITSVDIG SSESFLRGGE LPTLIVQSRG
501: HAVHVFINGQ LSGSAYGTRE YRRFMYTGKV NLRAGINRIA LLSVAIGLPN VGEHFESWST GILGPVALHG LDQGKWDLSG QKWTYQVGLK GEAMDLASPN
601: GISSVAWMQS AIVVQRNQPL TWHKTHFDAP EGDEPLALDM EGMGKGQIWI NGQSIGRYWT TFATGNCNDC NYAGSFRPPK CQLGCGQPTQ RWYHVPRSWL
701: KPTQNLLVIF EELGGNPSKI SLVKRSVSSV CADVSEYHPN IKNWHIESYG KSEEFHPPKV HLHCSPGQTI SSIKFASFGT PLGTCGNYEQ GACHSPASYA
801: ILEKRCIGKP RCTVTVSNSN FGQDPCPKVL KRLSVEAVCA PTAANWRG

Best Arabidopsis Sequence Match ( AT4G36360.1 )
(BLAST)

001: MREMGTGDSA SRLILWFCLG FLILGVGFVQ CGVTYDRKAL LINGQRRILF SGSIHYPRST PDMWEDLIQK AKDGGIDVIE TYVFWNLHEP SPGKYDFEGR
101: NDLVRFVKTI HKAGLYAHLR IGPYVCAEWN FGGFPVWLKY VPGISFRTDN EPFKRAMKGF TERIVELMKS ENLFESQGGP IILSQIENEY GRQGQLLGAE
201: GHNYMTWAAK MAIATETGVP WVMCKEDDAP DPVINTCNGF YCDSFAPNKP YKPLIWTEAW SGWFTEFGGP MHHRPVQDLA FGVARFIQKG GSFVNYYMYH
301: GGTNFGRTAG GPFVTTSYDY DAPIDEYGLI RQPKYGHLKE LHRAIKMCEK ALVSADPVVT SIGNKQQAHV YSAESGDCSA FLANYDTESA ARVLFNNVHY
401: NLPPWSISIL PDCRNAVFNT AKVGVQTSQM EMLPTDTKNF QWESYLEDLS SLDDSSTFTT HGLLEQINVT RDTSDYLWYM TSVDIGDSES FLHGGELPTL
501: IIQSTGHAVH IFVNGQLSGS AFGTRQNRRF TYQGKINLHS GTNRIALLSV AVGLPNVGGH FESWNTGILG PVALHGLSQG KMDLSWQKWT YQVGLKGEAM
601: NLAFPTNTPS IGWMDASLTV QKPQPLTWHK TYFDAPEGNE PLALDMEGMG KGQIWVNGES IGRYWTAFAT GDCSHCSYTG TYKPNKCQTG CGQPTQRWYH
701: VPRAWLKPSQ NLLVIFEELG GNPSTVSLVK RSVSGVCAEV SEYHPNIKNW QIESYGKGQT FHRPKVHLKC SPGQAIASIK FASFGTPLGT CGSYQQGECH
801: AATSYAILER KCVGKARCAV TISNSNFGKD PCPNVLKRLT VEAVCAPETS VSTWRP

Arabidopsis Description

BGAL3Beta-galactosidase 3 [Source:UniProtKB/Swiss-Prot;Acc:Q9SCV9]
SUBAcon: [endoplasmic reticulum,golgi,vacuole]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.