CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: extracellular, endoplasmic reticulum, vacuole, cytosol, plasma membrane, golgi

Predictor Summary:

nucleus 3
mitochondrion 1
extracellular 2
endoplasmic reticulum 2
vacuole 2
plasma membrane 2
golgi 2
cytosol 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol, secretory
Any Predictor:	cytosol, mitochondrion, nucleus, secretory
BaCelLo:	nucleus
MultiLoc:	nucleus
Plant-mPloc:	nucleus
Predotar:	secretory
PProwler:	mitochondrion
TargetP:	secretory
WoLF PSORT:	cytosol
YLoc:	cytosol

PPI

Inferred distinct locusB in Crop

locusB	locations
EER87819	nucleus
EES06214
EES07816
EES09179	nucleus
EES12410	nucleus
EES16550	nucleus
OQU87349

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G51600.1	EES07816	AT2G22640.1	25262777
AT5G51600.1	EER87819	AT3G19590.1	25262777
AT5G51600.1	EER87819	AT3G19590.1	25262777
AT5G51600.1	EES12410	AT3G25980.1	25262777
AT5G51600.1	EES12410	AT3G25980.1	25262777
AT5G51600.1	EES09179	AT5G05510.1	25262777
AT5G51600.1	EES16550	AT5G05510.1	25262777
AT5G51600.1	EES09179	AT5G05510.1	25262777
AT5G51600.1	EES16550	AT5G05510.1	25262777
AT5G51600.1	EES06214	AT5G51600.1	25262777
AT5G51600.1	OQU87349	AT5G51600.1	25262777

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Zm00001d015096_P002	Maize	nucleus	87.18	83.47
Zm00001d053956_P001	Maize	nucleus	83.93	82.38
Os02t0126300-01	Rice	plasma membrane	71.28	74.6
TraesCSU01G016800.1	Wheat	nucleus	59.49	63.27
TraesCS6A01G089500.2	Wheat	nucleus	59.15	63.14
TraesCS6B01G111900.1	Wheat	nucleus	58.63	62.48
Bra010069.1-P	Field mustard	nucleus	37.09	40.26
CDX86911	Canola	nucleus	37.09	40.26
CDY00270	Canola	nucleus	36.92	40.07
VIT_15s0048g00860.t01	Wine grape	cytosol, nucleus, plasma membrane	40.68	39.73
AT5G62250.1	Thale cress	nucleus	37.27	39.71
CDX87240	Canola	nucleus	35.9	38.82
Bra035900.1-P	Field mustard	nucleus	35.9	38.82
CDY16786	Canola	nucleus	35.9	38.67
VIT_16s0022g01820.t01	Wine grape	nucleus	46.67	37.4
EER88641	Sorghum	cytosol	36.75	37.07
KRH38719	Soybean	cytosol	40.34	36.65
OQU87349	Sorghum	golgi, vacuole	44.79	36.64
PGSC0003DMT400079660	Potato	cytosol	45.81	35.97
Solyc03g007130.2.1	Tomato	nucleus	45.3	35.76
PGSC0003DMT400070431	Potato	nucleus	42.22	35.59
KRH34346	Soybean	cytosol	45.13	35.44
KRG92454	Soybean	nucleus	43.93	35.35
KXG21994	Sorghum	cytosol	34.19	34.97
Bra023930.1-P	Field mustard	cytosol	42.05	34.7
AT5G51600.1	Thale cress	cytosol	41.88	34.65
Bra028266.1-P	Field mustard	cytosol	41.88	34.65
EER89674	Sorghum	cytosol	34.7	34.29
Solyc11g072280.1.1	Tomato	extracellular	44.1	33.46
EER98994	Sorghum	nucleus	32.99	32.38
Bra014467.1-P	Field mustard	cytosol	34.53	31.17
EES15312	Sorghum	cytosol	31.11	30.8
EER92583	Sorghum	cytosol	32.14	30.77
CDY62713	Canola	cytosol	35.9	30.7
KRH09228	Soybean	cytosol	28.03	30.65
CDX89081	Canola	cytosol	35.9	30.61
Solyc01g091380.2.1	Tomato	plastid	38.8	30.51
PGSC0003DMT400033224	Potato	nucleus	39.15	30.33
AT3G60840.1	Thale cress	cytosol	35.04	30.28
KRH40591	Soybean	nucleus	36.24	30.24
EER90980	Sorghum	cytosol	30.26	27.96
EES11428	Sorghum	nucleus	11.79	23.96
OQU77968	Sorghum	cytosol	12.14	19.35

Protein Annotations

Gene3D:1.20.58.1520	MapMan:35.1	EntrezGene:8055072	UniProt:C5XT70	ncoils:Coil	EnsemblPlants:EES06214
ProteinID:EES06214	ProteinID:EES06214.1	GO:GO:0000226	GO:GO:0000910	GO:GO:0003674	GO:GO:0005488
GO:GO:0005515	GO:GO:0007049	GO:GO:0008017	GO:GO:0008150	GO:GO:0009987	GO:GO:0016043
InterPro:MAP65_Ase1_PRC1	PFAM:PF03999	PANTHER:PTHR19321	PANTHER:PTHR19321:SF13	EnsemblPlantsGene:SORBI_3004G024800	UniParc:UPI0001A85F7B
RefSeq:XP_002453238.1	SEG:seg	:	:	:	:

Description

hypothetical protein

Coordinates

chr4:-:2012635..2015709

Molecular Weight (calculated)

65483.2 Da

IEP (calculated)

5.157

GRAVY (calculated)

-0.585

Length

585 amino acids

Sequence
(BLAST)

001: MAMLSLKLRA ILFPFQMEPR REELLHELGD MWDEIGEAED DRRGTLQALE EECLNVYRTK VDQVRQHRAQ LKREIADSVA EVAVICATIG EPPSTVHTAC
101: SSLQGTGSLK EELGLIAPEL AEMRRKREER RRQFADVTQR VNRMQEEMNL LVGQPNARAA AVVVDGSDLT TTKLDELRAY LHHLQQEKES RTKKVAELMA
201: QLHSSSLVLG MDSRETTNVV VNGGGVQVQA GDISDGAIAG LAAGIERLRE IKRSRMEKLQ DLLGSMLELW NLMDTPAEEQ RRFQGVACNI AASEDEITEP
301: GALSMDFIRN VEAEVVRLET LKECRMKDLV VKKHEELMEI RRRARLPEPE DDDAAVLLLD AIDSDAERAV ILERLEVQIS EAKDLEFSRK DVLEKMDRWQ
401: AALEEESWLE EYSRNENRYN VGKGTHLVLK RAEKARSLVS KMPAMAEALM AKVVAWEKER GAKFEYDGEV LLDMLEDYGN TRKEKEQERK RQRDQRRLLQ
501: QVQGAAAADR DVSSPLARPP PKNIKNVTRT LSMGGNAGGG GGGSARKAAA PASSRPGTPS FLKSPMSARR GGSDEGQMLS SDSFE

Best Arabidopsis Sequence Match ( AT5G62250.1 )
(BLAST)

001: MSKSQIESTW SSLLQELEII WKEVGETETE REKILIEIEE ECREVYNRKI EKVKEEKIRI KQEIADSEAR VIDICSVMEE PPILGRHHQS DQQSGNGRSL
101: KDELVKILQK LEEMEKRKSE RKIQFIQVID DIRCVREEIN GESDDETCSS DFSADESDLS LRKLEELHRE LYTLQEQKRN RVKQIQDNIR TLESLCSVLG
201: LNFRETVTKI HPSLVDTEGS RSISNETLDK LASSVQQWHE TKIQRMQELQ DLVTTMLEFW NLMDTPAEEQ QKFMDVSCNI AATVSEITKP NSLSIDLLEE
301: VKAELCRLEE LKWSKMKELV LKKRSELEEI CRRTHIVLEE EDIAVENVIK AIESGDVNPE NILEQIEYRA GKVKEEALSR KEILEKADKW LNACEEENWL
401: EEYNQDENRY NAGKGSHLIL KRAEKARALV NKLPAMVEAL ASKITIWESE KEYEFLFDGN RLLSMLEEYT ELREEKEQER RRKRDLKKHQ GQVTSEQDKG
501: SVTKPQSAKK GLKVSTNKRF VSSPHTPQTD SPHSAKSNQS FSTPLSRHG

Arabidopsis Description

MAP65-965-kDa microtubule-associated protein 9 [Source:UniProtKB/Swiss-Prot;Acc:Q4PSA3]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.