CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

extracellular 1
endoplasmic reticulum 1
vacuole 1
plasma membrane 1
golgi 1
plastid 1
nucleus 2
cytosol 3
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, mitochondrion, nucleus, plastid, secretory
BaCelLo:	secretory
ChloroP:	plastid
EpiLoc:	nucleus
MultiLoc:	cytosol
Plant-mPloc:	nucleus
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH57127	Soybean	endoplasmic reticulum, nucleus	94.68	93.14
GSMUA_Achr8P01340_001	Banana	cytosol	54.32	85.38
Solyc03g112350.2.1	Tomato	nucleus	76.58	70.81
EES04084	Sorghum	cytosol	67.77	70.59
AT5G63120.2	Thale cress	cytosol	68.94	70.22
KRH47127	Soybean	nucleus	59.14	70.22
HORVU3Hr1G092600.1	Barley	cytosol	66.78	70.03
HORVU3Hr1G092610.1	Barley	cytosol	66.78	70.03
TraesCS3A01G416900.1	Wheat	cytosol	66.78	70.03
KRH44155	Soybean	cytosol	58.97	70.02
TraesCS3D01G412400.2	Wheat	cytosol	66.78	69.91
TraesCS3B01G451900.1	Wheat	cytosol	66.61	69.86
Bra035857.1-P	Field mustard	cytosol	68.44	69.71
CDY48128	Canola	cytosol	68.77	69.7
CDY16735	Canola	cytosol	68.27	69.54
KRH48684	Soybean	cytosol, endoplasmic reticulum	50.83	64.29
Zm00001d042416_P002	Maize	extracellular, plasma membrane	67.44	63.64
VIT_17s0000g09130.t01	Wine grape	cytosol	63.95	63.01
PGSC0003DMT400046821	Potato	cytosol	74.92	62.81
KRH74291	Soybean	nucleus	32.72	36.69
KRH39630	Soybean	nucleus	32.56	36.5
KRG97325	Soybean	endoplasmic reticulum	32.89	33.5
KRH58901	Soybean	nucleus	33.89	33.22
KRH42811	Soybean	endoplasmic reticulum	34.05	33.12
KRH31589	Soybean	nucleus	33.22	33.11
KRH56577	Soybean	nucleus	42.52	33.03
KRH38030	Soybean	endoplasmic reticulum	33.39	32.9
KRH71800	Soybean	plastid	33.39	32.9
KRG93254	Soybean	endoplasmic reticulum	42.36	32.86
KRH20283	Soybean	plastid	31.73	32.59
KRH03709	Soybean	plastid	31.73	31.31
KRH30661	Soybean	endoplasmic reticulum	29.24	31.15
KRG98124	Soybean	endoplasmic reticulum	29.4	31.0
KRH68534	Soybean	cytosol	29.73	30.49
KRG96540	Soybean	cytosol, mitochondrion	38.7	30.34
KRG94409	Soybean	cytosol	29.57	30.27
KRH68277	Soybean	nucleus	38.54	29.67
KRH01937	Soybean	nucleus	29.4	25.07
KRH51212	Soybean	nucleus	28.74	24.68
KRH36905	Soybean	nucleus	43.52	22.49
KRH11875	Soybean	endoplasmic reticulum	43.69	21.9
KRH77709	Soybean	nucleus	37.54	20.47
KRH27757	Soybean	nucleus	37.38	20.33

Protein Annotations

EntrezGene:100817092	MapMan:16.4.1.1.4	Gene3D:3.40.50.300	EMBL:ACUP02010339	InterPro:DEAD/DEAH_box_helicase_dom	EnsemblPlantsGene:GLYMA_17G085300
GO:GO:0000166	GO:GO:0003674	GO:GO:0003676	GO:GO:0003824	GO:GO:0004004	GO:GO:0004386
GO:GO:0005488	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634
GO:GO:0005730	GO:GO:0005737	GO:GO:0006139	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987
GO:GO:0010501	GO:GO:0016787	InterPro:Helicase_ATP-bd	InterPro:Helicase_C	UniProt:I1MTD2	InterPro:IPR001650
InterPro:IPR014001	InterPro:IPR014014	EnsemblPlants:KRH03228	ProteinID:KRH03228	ProteinID:KRH03228.1	InterPro:P-loop_NTPase
PFAM:PF00270	PFAM:PF00271	ScanProsite:PS00039	PFscan:PS51192	PFscan:PS51194	PFscan:PS51195
PANTHER:PTHR24031	PANTHER:PTHR24031:SF269	InterPro:RNA-helicase_DEAD-box_CS	InterPro:RNA_helicase_DEAD_Q_motif	SMART:SM00487	SMART:SM00490
SUPFAM:SSF52540	UniParc:UPI000233F1E2	SEG:seg	:	:	:

Description

hypothetical protein

Coordinates

chr17:+:6576613..6581959

Molecular Weight (calculated)

65471.7 Da

IEP (calculated)

10.287

GRAVY (calculated)

-0.355

Length

602 amino acids

Sequence
(BLAST)

001: MNPYDNRYSD AASFRGRRSD FVGPIPPPSF VGRGGAVPYG VPGPNGFGSA PVAPVPPFVP PSGGFNVGRG GGRAGNGPVS DRKHDMGRGR GGGGGGRAGA
101: HGFRGSARGG GRHGGGSSRD DLNNIALPKQ DFKNLVPFEK NFYVECPAVR AMSEQEVLHY RASREITVQG NDVPKPIMMF HEANFPDYCL EVIANLRFAD
201: PTPIQAQGWP MALKGRDLIG IAETGSGKTL AYLLPALVHV NAQPRLAHGD GPIVLVLAPT RELAVQIQEE ALKFGSRANK RSTCIYGGAP KGPQIRELKR
301: GVEIVIATPG RLIDMLEAQH TNLRRVTYLV LDEADRMLDM GFEPQIRKIV AQIRPDRQTL LWSATWPRDV ETLARQFLHN PYKVIIGSPY LKANQSINQI
401: VEVVTDMEKY NRLIRLLKEV MDGSRILIFM ETKKGCDQVT RQMRVDGWPA LSIHGDKNQA ERDWVLAEFK SGRSPIMTAT DVAARGLDVK DIKCVINYDF
501: PTSLEDYVHR IGRTGRAGAK GTAYTFFTHA NAKFARDLIK ILQDAGQTVS PALTALVRSA GSGQFGSGGG FRSRGRGGYG NRGLTSGSNA IPLGSKRPWH
601: CL

Best Arabidopsis Sequence Match ( AT5G63120.2 )
(BLAST)

001: MSSYDRRFAD PNSYRQRSGA PVGSSQPMDP SAAPYNPRYT GGGGGYGPSP VMAGDNSGYN RYPSFQPPSG GFSVGRGGGR GGYGQYGDRN GGGNWGGGGG
101: RGGSSKRELD SVSLPKQNFG NLVHFEKNFY VESPTVQAMT EQDVAMYRTE RDISVEGRDV PKPMKMFQDA NFPDNILEAI AKLGFTEPTP IQAQGWPMAL
201: KGRDLIGIAE TGSGKTLAYL LPALVHVSAQ PRLGQDDGPI VLILAPTREL AVQIQEESRK FGLRSGVRST CIYGGAPKGP QIRDLRRGVE IVIATPGRLI
301: DMLECQHTNL KRVTYLVLDE ADRMLDMGFE PQIRKIVSQI RPDRQTLLWS ATWPREVETL ARQFLRDPYK AIIGSTDLKA NQSINQVIEI VPTPEKYNRL
401: LTLLKQLMDG SKILIFVETK RGCDQVTRQL RMDGWPALAI HGDKTQSERD RVLAEFKSGR SPIMTATDVA ARGLDVKDIK CVVNYDFPNT LEDYIHRIGR
501: TGRAGAKGMA FTFFTHDNAK FARELVKILQ EAGQVVPPTL SALVRSSGSG YGGSGGGRNF RPRGGGRGGG FGDKRSRSTS NFVPHGGKRT W

Arabidopsis Description

RH30DEAD-box ATP-dependent RNA helicase 30 [Source:UniProtKB/Swiss-Prot;Acc:Q8W4R3]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.