CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 3
cytosol 3
plastid 2
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRG93254	Soybean	endoplasmic reticulum	94.45	94.33
AT3G01540.2	Thale cress	cytosol	56.77	71.08
Bra040699.1-P	Field mustard	cytosol	55.48	70.61
Bra001004.1-P	Field mustard	cytosol	57.94	70.6
PGSC0003DMT400080614	Potato	nucleus	55.35	69.98
Solyc12g035130.1.1	Tomato	nucleus	55.1	69.77
Bra023478.1-P	Field mustard	cytosol	60.77	69.67
CDX91036	Canola	cytosol	60.0	67.98
Solyc02g086660.2.1	Tomato	nucleus	59.74	65.58
AT5G14610.1	Thale cress	cytosol	60.13	65.45
CDY28671	Canola	cytosol	55.74	65.16
CDX91943	Canola	cytosol	55.87	65.11
PGSC0003DMT400001391	Potato	cytosol	60.39	64.11
CDX74304	Canola	cytosol	55.74	62.79
CDX85591	Canola	cytosol	60.65	62.5
TraesCS3A01G130200.4	Wheat	cytosol	50.58	60.31
TraesCS3B01G149300.8	Wheat	cytosol	50.45	60.15
TraesCS3D01G131100.11	Wheat	cytosol	50.45	59.88
Zm00001d008725_P003	Maize	cytosol	52.9	59.16
VIT_14s0066g01020.t01	Wine grape	cytosol	65.81	59.1
Zm00001d039673_P002	Maize	cytosol	52.0	57.49
GSMUA_Achr8P31890_001	Banana	nucleus	58.06	57.25
OQU86230	Sorghum	cytosol	52.26	56.64
HORVU3Hr1G024610.13	Barley	cytosol, plastid	50.58	55.45
KXG32714	Sorghum	nucleus	56.13	54.99
Os01t0172200-01	Rice	cytosol	53.55	54.68
Zm00001d011155_P012	Maize	nucleus	54.71	54.15
Zm00001d044510_P002	Maize	mitochondrion	55.23	53.37
Os01t0549400-01	Rice	nucleus	54.32	53.16
TraesCS3A01G194200.1	Wheat	nucleus	51.87	51.34
TraesCS3D01G202500.3	Wheat	nucleus	53.55	51.3
TraesCS3B01G230800.1	Wheat	nucleus	51.48	50.83
KRH44155	Soybean	cytosol	30.58	46.75
KRH47127	Soybean	nucleus	30.58	46.75
KRH48684	Soybean	cytosol, endoplasmic reticulum	26.71	43.49
KRH03228	Soybean	nucleus	33.03	42.52
KRH57127	Soybean	endoplasmic reticulum, nucleus	33.16	41.99
KRH36905	Soybean	nucleus	55.1	36.65
KRH11875	Soybean	endoplasmic reticulum	55.23	35.64
KRG97325	Soybean	endoplasmic reticulum	27.1	35.53
KRH58901	Soybean	nucleus	27.87	35.18
KRH42811	Soybean	endoplasmic reticulum	27.87	34.9
KRH71800	Soybean	plastid	27.48	34.86
KRH38030	Soybean	endoplasmic reticulum	27.35	34.7
KRH31589	Soybean	nucleus	26.84	34.44
KRH74291	Soybean	nucleus	23.61	34.08
KRH20283	Soybean	plastid	25.68	33.96
KRH39630	Soybean	nucleus	23.48	33.89
KRH03709	Soybean	plastid	26.32	33.44
KRH30661	Soybean	endoplasmic reticulum	21.42	29.38
KRG98124	Soybean	endoplasmic reticulum	21.55	29.25
KRH68534	Soybean	cytosol	21.42	28.28
KRG94409	Soybean	cytosol	21.29	28.06
KRH51212	Soybean	nucleus	24.52	27.1
KRH01937	Soybean	nucleus	24.65	27.05
KRG96540	Soybean	cytosol, mitochondrion	25.68	25.91
KRH68277	Soybean	nucleus	25.42	25.19
KRH77709	Soybean	nucleus	25.03	17.57
KRH27757	Soybean	nucleus	24.9	17.43

Protein Annotations

EntrezGene:100786511	Gene3D:2.20.70.10	Gene3D:3.40.50.300	MapMan:35.1	EMBL:ACUP02002786	InterPro:DEAD/DEAH_box_helicase_dom
EnsemblPlantsGene:GLYMA_05G005500	GO:GO:0000166	GO:GO:0003674	GO:GO:0003676	GO:GO:0003824	GO:GO:0004004
GO:GO:0004386	GO:GO:0005488	GO:GO:0005515	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005634	GO:GO:0005730	GO:GO:0005737	GO:GO:0006139	GO:GO:0008150
GO:GO:0008152	GO:GO:0009987	GO:GO:0010501	GO:GO:0016787	InterPro:Helicase_ATP-bd	InterPro:Helicase_C
UniProt:I1K1I4	InterPro:IPR001202	InterPro:IPR001650	InterPro:IPR014001	InterPro:IPR014014	EnsemblPlants:KRH56577
ProteinID:KRH56577	ProteinID:KRH56577.1	InterPro:P-loop_NTPase	PFAM:PF00270	PFAM:PF00271	ScanProsite:PS00039
ScanProsite:PS01159	PFscan:PS50020	PFscan:PS51192	PFscan:PS51194	PFscan:PS51195	PANTHER:PTHR24031
PANTHER:PTHR24031:SF255	InterPro:RNA-helicase_DEAD-box_CS	InterPro:RNA_helicase_DEAD_Q_motif	SMART:SM00456	SMART:SM00487	SMART:SM00490
SUPFAM:SSF51045	SUPFAM:SSF52540	UniParc:UPI0002960656	InterPro:WW_dom	InterPro:WW_dom_sf	SEG:seg

Description

hypothetical protein

Coordinates

chr5:-:483665..487772

Molecular Weight (calculated)

83831.5 Da

IEP (calculated)

10.022

GRAVY (calculated)

-0.780

Length

775 amino acids

Sequence
(BLAST)

001: MAATATASSM GPRYAPADPT LPKPWRGLVD GKTGYLYFWN PETNVTQYER PSSSSTAQPK SSSVPNSSVQ VQQSSQGSQC GHSPDFSDRY DRNGSGGSNE
101: AGSRNNQKSS KGGSYASHDV SNGTHVAGNV DSSVRGHGAS DAGAGLSPES YRHRHEISVT GDNVPPPLAS FGSTGFPSEL LREVQNAGFS APTPIQAQSW
201: PIALQGRDIV AIAKTGSGKT LGYLVPAFIH LKRSGNNSKM GPTALVLSPT RELATQIQDE AVKFGKSSRI SCACLYGGAP KGPQLRDIDR GADIVVATPG
301: RLNDILEMRR ISLNQVSYLV LDEADRMLDM GFEPQIRKIV NEVPNRRQTL MFTATWPKEV RKIAADLLVK PVQVNIGNVD ELVANKSITQ HVEVLPPMEK
401: QRRLEHILRS QDSGSKIIIF CSTKKMCDQL ARNLTRQFGA AAIHGDKSQA ERDHVLNQFR TGRSPVLVAT DVAARGLDIK DIRVVVNYDF PTGVEDYVHR
501: IGRTGRAGAT GLAYTFFGDH DAKYASDLIK VLEGANQKVP PELRDMSSRS GGGMGRSRRW GSGGRGGDSG YGGRNNDSGY GGRGNDAGYG GRGSDSNYGG
601: RGTSASGRGG GRGFDYESQR NDRDRSPDKG SSWRDRFKSV NRDRSRSPNR AALPQHSQGG SFHKAMMERG GWDSDRNKSF NRDRSRSPSP HRQVKGPAVR
701: EHSPVYSFHR SMMGRGQSSP SSQRQRACSP HRNSTPSQGD GRHGGSHSSY NGEVEEGMIP EEEGMIGQDD PVNVD

Best Arabidopsis Sequence Match ( AT5G14610.2 )
(BLAST)

001: MAATASAIRY APEDPNLPKP WKGLVDSRTG YLYFWNPETN VTQYERPASS APPKLAAIPV SSSVQTNQQS SSGFNSGKED DKYGRGSDGP KSDSGSRFNE
101: AGRTGPISSN DAASGLGNAS SGGSSARGPP SSAAGNELSP EAYCRKHEIT VSGGQVPPPL MSFEATGLPN ELLREVYSAG FSAPSPIQAQ SWPIAMQNRD
201: IVAIAKTGSG KTLGYLIPGF MHLQRIHNDS RMGPTILVLS PTRELATQIQ VEALKFGKSS KISCACLYGG APKGPQLKEI ERGVDIVVAT PGRLNDILEM
301: KRISLHQVSY LVLDEADRML DMGFEPQIRK IVNEVPTKRQ TLMYTATWPK EVRKIAADLL VNPAQVNIGN VDELVANKSI TQTIEVLAPM EKHSRLEQIL
401: RSQEPGSKII IFCSTKRMCD QLARNLTRTF GAAAIHGDKS QAERDDVLNQ FRSGRTPVLV ATDVAARGLD VKDIRVVVNY DFPNGVEDYV HRIGRTGRAG
501: ATGLAYTFFG DQDAKHASDL IKILEGANQK VPPQVREMAT RGGGGMNKFR RWGTPSSGGG GGRGGYGDSG YGGRGESGYG SRGDSGYGGR GDSGGRGSWA
601: PSRDSSGSSG WGRERSRSPE RFRGGPPSTS SPPRSFHEAM MMKNR

Arabidopsis Description

DEAD box RNA helicase family protein [Source:UniProtKB/TrEMBL;Acc:F4K6V1]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.