CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

cytosol 4
mitochondrion 4

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
nucleus: 27291164
mitochondrion: 27780359
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27780359 doi

G Mustafa, S Komatsu

Graduate School of Life and Environmental Science, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27291164 doi

X Yin, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 27224218 doi

X Wang, S Komatsu

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH10294
KRH23029
KRH24840
KRH29844

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G14780.1	KRH10294	AT3G46090.1	17259181
AT5G14780.1	KRH23029	AT3G46090.1	17259181
AT5G14780.1	KRH24840	AT3G46090.1	17259181
AT5G14780.1	KRH29844	AT3G46090.1	17259181

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH20294	Soybean	cytosol, endoplasmic reticulum, mitochondrion, nucleus	97.43	97.68
KRG93315	Soybean	mitochondrion	86.12	89.33
Zm00001d011368_P001	Maize	cytosol	65.3	87.89
Zm00001d011367_P001	Maize	cytosol	65.3	87.89
Zm00001d006877_P001	Maize	cytosol, mitochondrion, plastid	63.24	87.23
Os06t0486800-01	Rice	mitochondrion	80.46	83.24
Zm00001d045965_P001	Maize	mitochondrion	80.21	82.98
EER89775	Sorghum	mitochondrion	80.21	82.98
TraesCS7D01G232300.1	Wheat	mitochondrion	79.69	82.23
TraesCS7A01G232200.1	Wheat	golgi	79.69	82.23
TraesCS7B01G130600.1	Wheat	mitochondrion	79.69	82.23
HORVU7Hr1G047700.3	Barley	mitochondrion, plasma membrane	79.18	81.7
VIT_14s0066g01320.t01	Wine grape	mitochondrion	79.95	81.2
Zm00001d053924_P001	Maize	cytosol	48.84	80.51
TraesCS4D01G007200.1	Wheat	golgi	76.86	79.73
Solyc02g086880.2.1	Tomato	plastid	77.89	79.53
PGSC0003DMT400001303	Potato	mitochondrion	77.89	79.53
GSMUA_Achr3P01200_001	Banana	mitochondrion	77.89	79.11
Os06t0486900-01	Rice	mitochondrion	76.86	79.1
Zm00001d014163_P001	Maize	mitochondrion	48.84	78.51
TraesCS4B01G066400.1	Wheat	mitochondrion	71.21	78.03
TraesCS6B01G302400.2	Wheat	mitochondrion	75.58	77.98
AT5G14780.1	Thale cress	mitochondrion	76.86	77.86
TraesCS6D01G255200.2	Wheat	mitochondrion	75.32	77.72
Bra008737.1-P	Field mustard	mitochondrion	76.09	77.08
CDY09885	Canola	mitochondrion	75.84	77.02
CDX69558	Canola	mitochondrion	75.84	76.82
EES07384	Sorghum	mitochondrion	75.58	76.56
CDX85582	Canola	mitochondrion	74.55	75.72
Bra023487.1-P	Field mustard	mitochondrion	74.29	75.26
CDX91020	Canola	mitochondrion	73.52	74.48
Zm00001d004323_P001	Maize	mitochondrion	24.42	65.52
TraesCS5D01G389600.1	Wheat	extracellular, mitochondrion	61.7	65.4
Zm00001d019818_P001	Maize	mitochondrion	23.65	63.45
Zm00001d017801_P001	Maize	mitochondrion	27.76	62.79
Zm00001d033618_P001	Maize	cytosol	60.93	61.56
TraesCS6A01G274900.1	Wheat	mitochondrion	59.64	61.54
KRH44352	Soybean	peroxisome	10.28	26.14
KRH48310	Soybean	cytosol	19.28	22.46
KRH47286	Soybean	nucleus	17.99	22.36
KRH09880	Soybean	cytosol	16.71	21.45
KRH62463	Soybean	mitochondrion	21.08	20.97
KRH48311	Soybean	cytosol	17.99	20.96
KRH48312	Soybean	cytosol	17.48	20.18
KRH36064	Soybean	nucleus	19.54	19.69
KRG90684	Soybean	nucleus	19.54	19.69
KRH48313	Soybean	cytosol	16.97	19.64
KRH65201	Soybean	plastid	13.88	18.82
KRH65202	Soybean	cytosol	10.03	17.33
KRH35731	Soybean	nucleus	21.59	14.14
KRH44645	Soybean	nucleus	22.11	13.85
KRG91030	Soybean	endoplasmic reticulum	21.08	13.78
KRH47008	Soybean	endoplasmic reticulum	21.85	13.64
KRH23702	Soybean	extracellular	20.31	13.48

Protein Annotations

KEGG:00630+1.17.1.9	KEGG:00680+1.17.1.9	EntrezGene:100797606	Gene3D:3.40.50.720	MapMan:50.1.16	EMBL:ACUP02011548
InterPro:D-isomer_2_OHA_DH_cat_dom	InterPro:D-isomer_DH_CS	InterPro:D-isomer_DH_CS1	InterPro:D-isomer_DH_NAD-bd	InterPro:FDH_NAD-dep	EnsemblPlantsGene:GLYMA_19G009100
GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0005488	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0005739	GO:GO:0008150	GO:GO:0008152	GO:GO:0008863
GO:GO:0009056	GO:GO:0009987	GO:GO:0016491	GO:GO:0016616	GO:GO:0042183	GO:GO:0051287
GO:GO:0055114	UniProt:I1N5S0	EnsemblPlants:KRG93317	ProteinID:KRG93317	ProteinID:KRG93317.1	HAMAP:MF_03210
InterPro:NAD(P)-bd_dom_sf	PFAM:PF00389	PFAM:PF02826	ScanProsite:PS00065	ScanProsite:PS00670	PANTHER:PTHR42938
PANTHER:PTHR42938:SF1	MetaCyc:PWY-1881	MetaCyc:PWY-5497	MetaCyc:PWY-6696	SUPFAM:SSF51735	SUPFAM:SSF52283
UniParc:UPI00023D83ED	SEG:seg	:	:	:	:

Description

hypothetical protein

Coordinates

chr19:+:867635..870397

Molecular Weight (calculated)

42862.2 Da

IEP (calculated)

6.760

GRAVY (calculated)

-0.307

Length

389 amino acids

Sequence
(BLAST)

001: MSDPTLAQQH LVKVHTTTHE TVVTTHNHNQ TPSINASGEK KKIVGVFYKG NEYAKLNPNF VGCVEGALGI REWLESQGHQ YIVTDDKEGP DSELEKHIPD
101: AHVIISTPFH PAYVTAERIK KAKNLELLLT AGIGSDHVDL KAAAAAGLTV AEVTGSNVVS VAEDELMRIL ILMRNFLPGY HQAVNGEWNV AGIAHRAYDL
201: EGKTVGTVGA GRIGKLLLQR LKPFSCNLLY FDRLRIDPEL EKEIGAKFEE DLDAMLPKCD VIVINTPLTE QTRGLFDKNR IAKCKKGVLI VNNARGAIAD
301: TQAIADACSS GHVAGYSGDV WFPQPAPKDH PWRYMPNHAM TPHISGTTID AQLRYAAGVK DMLDRHFKGE DFPEQNYIVK EGQLASQYR

Best Arabidopsis Sequence Match ( AT5G14780.1 )
(BLAST)

001: MAMRQAAKAT IRACSSSSSS GYFARRQFNA SSGDSKKIVG VFYKANEYAT KNPNFLGCVE NALGIRDWLE SQGHQYIVTD DKEGPDCELE KHIPDLHVLI
101: STPFHPAYVT AERIKKAKNL KLLLTAGIGS DHIDLQAAAA AGLTVAEVTG SNVVSVAEDE LMRILILMRN FVPGYNQVVK GEWNVAGIAY RAYDLEGKTI
201: GTVGAGRIGK LLLQRLKPFG CNLLYHDRLQ MAPELEKETG AKFVEDLNEM LPKCDVIVIN MPLTEKTRGM FNKELIGKLK KGVLIVNNAR GAIMERQAVV
301: DAVESGHIGG YSGDVWDPQP APKDHPWRYM PNQAMTPHTS GTTIDAQLRY AAGTKDMLER YFKGEDFPTE NYIVKDGELA PQYR

Arabidopsis Description

FDH1Formate dehydrogenase, mitochondrial [Source:UniProtKB/TrEMBL;Acc:A0A178UHK7]
SUBAcon: [mitochondrion]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.