CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 2
vacuole 1
cytosol 2
plastid 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH10858
KRH11411
KRH20872
KRH20873
KRH31624
KRH31641
KRH31820
KRH53154
KRH64753

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G20280.1	KRH31624	AT1G02680.1	21798944
AT5G20280.1	KRH31641	AT1G02680.1	21798944
AT5G20280.1	KRH31820	AT1G02680.1	21798944
AT5G20280.1	KRH11411	AT1G61870.1	21798944
AT5G20280.1	KRH20872	AT1G61870.1	21798944
AT5G20280.1	KRH20873	AT1G61870.1	21798944
AT5G20280.1	KRH10858	AT4G21705.1	21798944
AT5G20280.1	KRH53154	AT5G09320.1	21798944
AT5G20280.1	KRH64753	AT5G09320.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH03629	Soybean	endoplasmic reticulum	97.26	97.26
VIT_11s0118g00200.t01	Wine grape	cytosol	82.53	83.08
CDY35815	Canola	cytosol	72.52	79.5
CDX92473	Canola	cytosol	72.43	79.4
Solyc07g007790.2.1	Tomato	cytosol	77.9	78.27
Bra002289.1-P	Field mustard	cytosol	76.96	78.22
PGSC0003DMT400071807	Potato	cytosol	77.71	78.08
AT5G20280.1	Thale cress	cytosol	76.39	77.56
CDY45096	Canola	cytosol	70.73	77.06
Bra020096.1-P	Field mustard	cytosol	73.65	75.73
GSMUA_Achr4P06050_001	Banana	cytosol	73.37	73.37
GSMUA_Achr9P22510_001	Banana	cytosol	72.99	72.86
Os08t0301500-01	Rice	plasma membrane	68.84	68.39
OQU78428	Sorghum	nucleus	68.46	67.76
Zm00001d050125_P002	Maize	nucleus	67.52	67.52
TraesCS3D01G013000.1	Wheat	nucleus	67.61	66.98
TraesCS3A01G015500.1	Wheat	golgi	67.23	66.48
TraesCSU01G044500.2	Wheat	unclear	67.33	66.26
TraesCS6A01G144800.1	Wheat	cytoskeleton, cytosol, nucleus	58.07	63.8
TraesCS6D01G133800.1	Wheat	cytosol	57.7	63.38
TraesCS7A01G518200.1	Wheat	cytosol	58.07	63.27
TraesCS7B01G434300.1	Wheat	cytosol	57.98	63.23
TraesCS7D01G508100.1	Wheat	cytosol	57.98	63.23
HORVU3Hr1G002280.1	Barley	plastid	67.42	63.19
Zm00001d015570_P002	Maize	cytosol	57.41	63.07
KXG29640	Sorghum	cytosol	57.32	62.97
Zm00001d036749_P001	Maize	cytosol	57.51	62.46
KXG20466	Sorghum	cytosol	57.22	61.84
Os02t0184400-01	Rice	endoplasmic reticulum, golgi	57.51	60.24
HORVU6Hr1G028330.1	Barley	endoplasmic reticulum, golgi	58.36	59.88
TraesCS6B01G173000.2	Wheat	endoplasmic reticulum, plasma membrane	58.17	59.23
KRH46034	Soybean	cytosol	56.56	56.78
KRG98934	Soybean	cytosol	56.28	56.6
HORVU7Hr1G115590.10	Barley	nucleus	53.26	56.46
KRH14493	Soybean	cytosol	56.47	56.26
Zm00001d046595_P002	Maize	cytosol	51.37	55.62
KRH56446	Soybean	cytosol	52.69	53.81
KRH62455	Soybean	cytosol	38.9	53.02
Os06t0643800-01	Rice	plasma membrane	9.07	52.17
Zm00001d016399_P002	Maize	extracellular	6.99	47.13
KRG96468	Soybean	cytosol, endoplasmic reticulum, nucleus	16.9	22.04
KRH68215	Soybean	cytosol, endoplasmic reticulum, nucleus	16.9	22.04
KRH12090	Soybean	endoplasmic reticulum	16.62	21.95
KRH19379	Soybean	cytosol	16.43	21.61
KRH02557	Soybean	cytosol, endoplasmic reticulum, extracellular, mitochondrion	16.34	21.49
KRH12615	Soybean	endoplasmic reticulum	16.34	21.46
KRH37557	Soybean	endoplasmic reticulum	16.24	21.23
KRH72916	Soybean	cytosol	16.43	20.71
KRH17270	Soybean	cytosol	16.34	20.6
KRH30890	Soybean	cytosol	15.86	19.88
KRH09470	Soybean	cytosol, plastid	14.83	17.07
KRH38924	Soybean	plastid	14.83	17.05

Protein Annotations

KEGG:00500+2.4.1.14	EntrezGene:100127429	MapMan:3.1.2.7	Gene3D:3.40.50.1000	Gene3D:3.40.50.2000	Gene3D:3.90.1070.10
EMBL:ACUP02008246	EnsemblPlantsGene:GLYMA_13G161600	GO:GO:0003674	GO:GO:0003824	GO:GO:0005975	GO:GO:0005985
GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0016157	GO:GO:0016740	GO:GO:0016757
GO:GO:0046524	InterPro:Glyco_trans_1	InterPro:HAD_sf	InterPro:IPR023214	UniProt:K7M052	EnsemblPlants:KRH20178
ProteinID:KRH20178	ProteinID:KRH20178.1	PFAM:PF00534	PFAM:PF00862	PFAM:PF05116	PANTHER:PTHR12526
PANTHER:PTHR12526:SF334	MetaCyc:PWY-7238	MetaCyc:PWY-7347	InterPro:SPP_N	InterPro:SPS_C	SUPFAM:SSF53756
InterPro:Sucrose_synth	InterPro:SucrsPsyn_pln	TIGRFAMs:TIGR02468	UniParc:UPI000233B399	SEG:seg	:

Description

hypothetical protein

Coordinates

chr13:-:27715861..27722364

Molecular Weight (calculated)

118018.0 Da

IEP (calculated)

6.425

GRAVY (calculated)

-0.445

Length

1059 amino acids

Sequence
(BLAST)

0001: MAGNDWLNSY LEAILDVGPG LDDAKSSLLL RERGRFSPTR YFVQEVIGFD ETDLYRSWVR ASSTRSPQER NTRLENMCWR IWNLARQKKQ LESETALRVN
0101: KRRLERERGR REATADMSED LSEGEKGDPL SDLSAHGGVG DFNRSRLPRI SSADAMETWA NSQKGKKLYI VLISIHGLIR GENMELGRDS DTGGQVKYVV
0201: ELARALGSMP GVYRVDLLTR QVSAPDVDWS YGEPTEMLSP RDTDDFGDDT GESSGSYIVR IPFGPRDKYI PKELLWPYIP EFVDGALNHI IQMSKSLGEQ
0301: IGSGHAVWPV AIHGHYADAG DSAALLSGAL NVPMLFTGHS LGRDKLEQLL KQGRLSKDEI NTTYKIMRRI EAEELALDGS EIVITSTRQE IEEQWRLYDG
0401: FDPVLERKLR ARIRRNVSCY GRFMPRMATI PPGMEFHHIV PHDGDIEGEP EGNLDHPAPQ DPPIWSEIMR FFTNPRKPMI LALARPDPKK NITTLVKAFG
0501: ECRPLQELAN LTLIMGNRDG IDEMSSTNAS VLLSVLKLID KYDLYGQVAY PKHHKQYDVP DIYRLAAKTK GVFINPAFIE PFGLTLIEAA AHGLPIVATK
0601: NGGPVDIHRV LDNGLLVDPH DQQSIADALL KLVSNKQLWA KCRQNGLKNI HLFSWPEHCK TYLSKIATCK PRHPQWQRSE DGGESSESDS PGDSLRDLQD
0701: LSLNLKFSLD GEKSEGSGND NSLNSDGNAA DRGAKLENAV LSWSKGISKD TRRGGATEKS DQNPNAGKFP PLRRRKHLFV IAVDCDTTSS LLETIKAIFE
0801: SAGKDRAEST VGFILSTSLT ISEIQSFLIS GGLSPIDFDA YICNSGSDLY YPSLNPGDRP FVVDLYYHSH IEYRWGGEGL RKTLVRWADS ITDKKGDNDE
0901: QIVSPAEQLS TDYCYAFKVR KPGMAPPVKE LRKLLRIQAL RCHPIYCQNG TRLNVIPVLA SRSQALRYLY VRWGFELSKM VVFVGECGDT DYEGLLGGLH
1001: KSVILKGVGS SAISQLHNNR SYPLSDVTPL DSPNIVEATE GSSGADIQAL IEKVGYLNG

Best Arabidopsis Sequence Match ( AT5G20280.1 )
(BLAST)

0001: MAGNDWVNSY LEAILDVGQG LDDARSSPSL LLRERGRFTP SRYFVEEVIT GYDETDLHRS WVKAVATRSP QERNTRLENM CWRIWNLARQ KKQHEEKEAQ
0101: RLAKRRLERE KGRREATADM SEEFSEGEKG DIISDISTHG ESTKPRLPRI NSAESMELWA SQQKGNKLYL VLISLHGLIR GENMELGRDS DTGGQVKYVV
0201: ELARALGSMP GVYRVDLLTR QVSSPDVDYS YGEPTEMLTP RDSEDFSDEM GESSGAYIVR IPFGPKDKYI PKELLWPHIP EFVDGAMSHI MQMSNVLGEQ
0301: VGVGKPIWPS AIHGHYADAG DATALLSGAL NVPMLLTGHS LGRDKLEQLL RQGRLSKEEI NSTYKIMRRI EGEELSLDVS EMVITSTRQE IDEQWRLYDG
0401: FDPILERKLR ARIKRNVSCY GRFMPRMVKI PPGMEFNHIV PHGGDMEDTD GNEEHPTSPD PPIWAEIMRF FSNSRKPMIL ALARPDPKKN ITTLVKAFGE
0501: CRPLRELANL ALIMGNRDGI DEMSSTSSSV LLSVLKLIDK YDLYGQVAYP KHHKQSDVPD IYRLAAKSKG VFINPAIIEP FGLTLIEAAA HGLPMVATKN
0601: GGPVDIHRVL DNGLLVDPHD QQSISEALLK LVADKHLWAK CRQNGLKNIH QFSWPEHCKT YLSRITSFKP RHPQWQSDDG GDNSEPESPS DSLRDIQDIS
0701: LNLKFSFDGS GNDNYMNQEG SSMDRKSKIE AAVQNWSKGK DSRKMGSLER SEVNSGKFPA VRRRKFIVVI ALDFDGEEDT LEATKRILDA VEKERAEGSV
0801: GFILSTSLTI SEVQSFLVSG GLNPNDFDAF ICNSGSDLHY TSLNNEDGPF VVDFYYHSHI EYRWGGEGLR KTLIRWASSL NEKKADNDEQ IVTLAEHLST
0901: DYCYTFTVKK PAAVPPVREL RKLLRIQALR CHVVYSQNGT RINVIPVLAS RIQALRYLFV RWGIDMAKMA VFVGESGDTD YEGLLGGLHK SVVLKGVSCS
1001: ACLHANRSYP LTDVISFESN NVVHASPDSD VRDALKKLEL LKD

Arabidopsis Description

SPS1Sucrose-phosphate synthase 1 [Source:UniProtKB/Swiss-Prot;Acc:Q94BT0]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.