CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

extracellular 6
endoplasmic reticulum 6
vacuole 6
plasma membrane 6
golgi 5
cytosol 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, secretory
BaCelLo:	secretory
iPSORT:	secretory
MultiLoc:	endoplasmic reticulum
Plant-mPloc:	extracellular, plasma membrane
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	cytosol
YLoc:	vacuole

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH42483
KRH58580

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G16370.1	KRH42483	AT2G01950.1	19000166
AT3G16370.1	KRH58580	AT2G01950.1	19000166

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH09971	Soybean	nucleus	88.95	91.81
KRH47190	Soybean	nucleus	83.85	83.85
KRH44252	Soybean	nucleus	83.57	80.82
VIT_09s0002g03140.t01	Wine grape	extracellular	77.34	76.69
Solyc03g121180.2.1	Tomato	nucleus	76.2	74.93
PGSC0003DMT400006459	Potato	extracellular	76.2	74.93
Bra027168.1-P	Field mustard	extracellular	72.24	72.03
CDY30167	Canola	extracellular	72.24	72.03
AT3G16370.1	Thale cress	endoplasmic reticulum, vacuole	71.95	71.95
CDY56196	Canola	vacuole	72.52	71.51
PGSC0003DMT400006461	Potato	extracellular	69.97	68.8
GSMUA_Achr7P08930_001	Banana	endoplasmic reticulum, extracellular	68.27	67.13
Os06t0148200-01	Rice	extracellular	66.57	66.95
GSMUA_Achr2P16800_001	Banana	extracellular, vacuole	66.29	65.36
VIT_09s0002g03150.t01	Wine grape	extracellular	64.59	64.23
EER87840	Sorghum	extracellular, vacuole	64.31	63.06
Zm00001d011661_P001	Maize	extracellular	63.74	63.03
TraesCS4A01G397000.1	Wheat	extracellular	62.32	62.68
TraesCS7D01G093200.1	Wheat	extracellular	62.32	62.68
TraesCS7A01G097100.1	Wheat	golgi	62.04	62.39
KRH26558	Soybean	endoplasmic reticulum, extracellular, vacuole	58.07	58.57
Solyc03g121170.2.1	Tomato	nucleus	69.12	58.51
KRH30531	Soybean	extracellular, vacuole	58.36	58.36
KRH25150	Soybean	extracellular	48.73	56.21
HORVU7Hr1G021310.7	Barley	extracellular	62.61	56.09
HORVU7Hr1G021630.2	Barley	cytosol, plastid	41.64	54.24
HORVU7Hr1G021580.1	Barley	extracellular	53.54	53.54
TraesCS7D01G094000.1	Wheat	extracellular	52.97	53.28
HORVU7Hr1G021460.4	Barley	extracellular	52.97	53.12
TraesCS7D01G094500.1	Wheat	extracellular	52.97	52.97
TraesCS4A01G398500.1	Wheat	plasma membrane	52.97	52.97
TraesCS4A01G397900.1	Wheat	extracellular	52.97	52.82
TraesCS4A01G398700.1	Wheat	extracellular	53.26	52.51
TraesCS7A01G099900.1	Wheat	extracellular, golgi, plasma membrane	53.26	52.37
TraesCS4A01G398600.1	Wheat	extracellular	51.84	51.84
Zm00001d036175_P001	Maize	endoplasmic reticulum, extracellular, vacuole	51.84	50.97
EER87852	Sorghum	extracellular	51.84	50.69
TraesCS4A01G399300.1	Wheat	endoplasmic reticulum	51.84	50.55
TraesCS7D01G094900.1	Wheat	endoplasmic reticulum, golgi	51.27	50.0
HORVU2Hr1G002340.1	Barley	cytosol, plastid	36.26	49.61
HORVU7Hr1G021620.2	Barley	endoplasmic reticulum, golgi, mitochondrion	51.56	49.46
TraesCS7A01G100600.1	Wheat	endoplasmic reticulum, golgi	51.27	49.45
KRH22747	Soybean	mitochondrion	59.49	49.41
TraesCS3D01G431500.1	Wheat	endoplasmic reticulum, golgi	52.12	46.58
TraesCS7A01G100200.1	Wheat	extracellular	43.34	45.27
Os06t0149100-01	Rice	cytosol	20.68	44.51
CDX75880	Canola	vacuole	38.24	43.55
KRG89977	Soybean	cytosol	15.3	43.2
HORVU0Hr1G019420.1	Barley	endoplasmic reticulum, mitochondrion	28.05	42.67
KRH61021	Soybean	mitochondrion	17.28	38.12
KRH17464	Soybean	extracellular	37.39	35.97
KRH05981	Soybean	extracellular	36.83	35.52
KRH72762	Soybean	cytosol	16.15	32.39

Protein Annotations

EntrezGene:100795716	Gene3D:3.40.50.1110	MapMan:35.1	EMBL:ACUP02008462	InterPro:GDSL	EnsemblPlantsGene:GLYMA_13G353100
GO:GO:0003674	GO:GO:0003824	GO:GO:0016787	GO:GO:0016788	UniProt:I1M5F3	InterPro:IPR036514
EnsemblPlants:KRH23361	ProteinID:KRH23361	ProteinID:KRH23361.1	PFAM:PF00657	PANTHER:PTHR22835	PANTHER:PTHR22835:SF134
InterPro:SGNH_hydro_sf	InterPro:SGNH_plant_lipase_like	SUPFAM:SSF52266	SignalP:SignalP-noTM	UniParc:UPI000233B3BA	SEG:seg

Description

hypothetical protein

Coordinates

chr13:+:44189998..44191915

Molecular Weight (calculated)

38135.9 Da

IEP (calculated)

9.509

GRAVY (calculated)

0.076

Length

353 amino acids

Sequence
(BLAST)

001: MNLNSTEALL VLFAFVFLAW GNAQNTLVPA IITFGDSAVD VGNNDYLPTL FKANYPPYGR DFINHQPTGR FCNGKLATDI TAETLGFKSY APAYLSPQAS
101: GKNLLIGANF ASAASGYDEK AAILNHAIPL SQQLKYYKEY RGKLAKVVGS KKAALIIKNA LYILSAGSSD FVQNYYVNPL INKAFTPDQY SAYLVGSFSS
201: FVKDLYKLGA RKVGVTSLPP LGCLPAARTL FSFHEKGCVS RINNDTQGFN KKIKSAAANL QKQLPGLKIV VFDIFKPLYD LVQSPSKFGF AEARKGCCGT
301: GIVETTSLLC NPKSLGTCSN ATQYVFWDSV HPSQAANQVL ADALIVQGIA LIT

Best Arabidopsis Sequence Match ( AT3G16370.1 )
(BLAST)

001: MDRCTSSFLL LTLVSTLSIL QISFAQLVPA IMTFGDSVVD VGNNNYLPTL FRADYPPYGR DFANHKATGR FCNGKLATDI TAETLGFTKY PPAYLSPEAS
101: GKNLLIGANF ASAASGYDDK AALLNHAIPL YQQVEYFKEY KSKLIKIAGS KKADSIIKGA ICLLSAGSSD FVQNYYVNPL LYKVYTVDAY GSFLIDNFST
201: FIKQVYAVGA RKIGVTSLPP TGCLPAARTL FGFHEKGCVS RLNTDAQNFN KKLNAAASKL QKQYSDLKIV VFDIYSPLYD LVQNPSKSGF TEATKGCCGT
301: GTVETTSLLC NPKSFGTCSN ATQYVFWDSV HPSEAANEIL ATALIGQGFS LLG

Arabidopsis Description

APGGDSL esterase/lipase APG [Source:UniProtKB/Swiss-Prot;Acc:Q9LU14]
SUBAcon: [endoplasmic reticulum,vacuole]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.