CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

extracellular 8
endoplasmic reticulum 5
vacuole 6
plasma membrane 5
golgi 5

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	secretory
BaCelLo:	secretory
iPSORT:	secretory
MultiLoc:	extracellular
Plant-mPloc:	extracellular
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	extracellular
YLoc:	vacuole

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH42483
KRH58580

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G16370.1	KRH42483	AT2G01950.1	19000166
AT3G16370.1	KRH58580	AT2G01950.1	19000166

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH44252	Soybean	nucleus	96.88	93.7
KRH23361	Soybean	nucleus	83.85	83.85
KRH09971	Soybean	nucleus	78.47	80.99
VIT_09s0002g03140.t01	Wine grape	extracellular	79.6	78.93
PGSC0003DMT400006459	Potato	extracellular	78.75	77.44
Solyc03g121180.2.1	Tomato	nucleus	78.47	77.16
AT3G16370.1	Thale cress	endoplasmic reticulum, vacuole	72.8	72.8
Bra027168.1-P	Field mustard	extracellular	71.67	71.47
CDY30167	Canola	extracellular	71.67	71.47
PGSC0003DMT400006461	Potato	extracellular	72.52	71.31
CDY56196	Canola	vacuole	71.67	70.67
GSMUA_Achr7P08930_001	Banana	endoplasmic reticulum, extracellular	69.97	68.8
GSMUA_Achr2P16800_001	Banana	extracellular, vacuole	69.12	68.16
Os06t0148200-01	Rice	extracellular	67.42	67.81
EER87840	Sorghum	extracellular, vacuole	66.01	64.72
Zm00001d011661_P001	Maize	extracellular	64.87	64.15
TraesCS7D01G093200.1	Wheat	extracellular	63.74	64.1
TraesCS4A01G397000.1	Wheat	extracellular	63.46	63.82
TraesCS7A01G097100.1	Wheat	golgi	63.46	63.82
VIT_09s0002g03150.t01	Wine grape	extracellular	63.46	63.1
Solyc03g121170.2.1	Tomato	nucleus	71.39	60.43
KRH30531	Soybean	extracellular, vacuole	58.92	58.92
KRH26558	Soybean	endoplasmic reticulum, extracellular, vacuole	56.94	57.43
HORVU7Hr1G021310.7	Barley	extracellular	64.02	57.36
HORVU7Hr1G021630.2	Barley	cytosol, plastid	43.91	57.2
KRH25150	Soybean	extracellular	47.59	54.9
HORVU7Hr1G021460.4	Barley	extracellular	54.39	54.55
TraesCS7D01G094500.1	Wheat	extracellular	54.11	54.11
TraesCS7D01G094000.1	Wheat	extracellular	53.54	53.85
TraesCS4A01G398500.1	Wheat	plasma membrane	53.82	53.82
TraesCS7A01G099900.1	Wheat	extracellular, golgi, plasma membrane	54.39	53.48
TraesCS4A01G398700.1	Wheat	extracellular	54.11	53.35
HORVU7Hr1G021580.1	Barley	extracellular	53.26	53.26
TraesCS4A01G397900.1	Wheat	extracellular	52.97	52.82
Zm00001d036175_P001	Maize	endoplasmic reticulum, extracellular, vacuole	53.54	52.65
EER87852	Sorghum	extracellular	53.82	52.63
HORVU2Hr1G002340.1	Barley	cytosol, plastid	37.96	51.94
TraesCS4A01G398600.1	Wheat	extracellular	51.56	51.56
TraesCS4A01G399300.1	Wheat	endoplasmic reticulum	51.84	50.55
TraesCS7D01G094900.1	Wheat	endoplasmic reticulum, golgi	51.56	50.28
HORVU7Hr1G021620.2	Barley	endoplasmic reticulum, golgi, mitochondrion	52.41	50.27
TraesCS7A01G100600.1	Wheat	endoplasmic reticulum, golgi	51.27	49.45
KRH22747	Soybean	mitochondrion	58.07	48.24
TraesCS3D01G431500.1	Wheat	endoplasmic reticulum, golgi	52.41	46.84
TraesCS7A01G100200.1	Wheat	extracellular	44.48	46.45
Os06t0149100-01	Rice	cytosol	20.4	43.9
CDX75880	Canola	vacuole	37.39	42.58
HORVU0Hr1G019420.1	Barley	endoplasmic reticulum, mitochondrion	27.76	42.24
KRG89977	Soybean	cytosol	14.73	41.6
KRH61021	Soybean	mitochondrion	17.56	38.75
KRH05981	Soybean	extracellular	38.53	37.16
KRH17464	Soybean	extracellular	37.96	36.51
KRH72762	Soybean	cytosol	17.85	35.8

Protein Annotations

Gene3D:3.40.50.1110	MapMan:35.1	EMBL:ACUP02004139	InterPro:GDSL	EnsemblPlantsGene:GLYMA_07G014100	GO:GO:0003674
GO:GO:0003824	GO:GO:0016787	GO:GO:0016788	UniProt:I1KGH3	InterPro:IPR036514	EnsemblPlants:KRH47190
ProteinID:KRH47190	ProteinID:KRH47190.1	PFAM:PF00657	PANTHER:PTHR22835	PANTHER:PTHR22835:SF170	InterPro:SGNH_hydro_sf
InterPro:SGNH_plant_lipase_like	SUPFAM:SSF52266	SignalP:SignalP-noTM	TMHMM:TMhelix	UniParc:UPI00023390B0	SEG:seg

Description

hypothetical protein

Coordinates

chr7:+:1106615..1108895

Molecular Weight (calculated)

37825.8 Da

IEP (calculated)

7.959

GRAVY (calculated)

0.044

Length

353 amino acids

Sequence
(BLAST)

001: MINIGALVVL FAFLFLSCAY AQDTTTLVPA IITFGDSAVD VGNNDYLPTL FKADYPPYGR DFANHQPTGR FCNGKLATDF TADTLGFKTY APAYLSPQAS
101: GKNLLIGANF ASAASGYDEN AATLNHAIPL SQQLSYFKEY QGKLAKVAGS KKAASIIKDA LYVLSAGSSD FVQNYYVNPW INKVYSPDQY SSYLVGEFSS
201: FVKDLYGLGA RRLGVTSLPP LGCLPAARTI FGFHENGCVS RINTDAQGFN KKLNSAAASL QKQLPGLKIA IFDIYKPLYD LVQSPSKSGF VEANRGCCGT
301: GTVETTSLLC NSKSPGTCSN ATQYVFWDSV HPSQAANQVL ADALILQGIS LVT

Best Arabidopsis Sequence Match ( AT3G16370.1 )
(BLAST)

001: MDRCTSSFLL LTLVSTLSIL QISFAQLVPA IMTFGDSVVD VGNNNYLPTL FRADYPPYGR DFANHKATGR FCNGKLATDI TAETLGFTKY PPAYLSPEAS
101: GKNLLIGANF ASAASGYDDK AALLNHAIPL YQQVEYFKEY KSKLIKIAGS KKADSIIKGA ICLLSAGSSD FVQNYYVNPL LYKVYTVDAY GSFLIDNFST
201: FIKQVYAVGA RKIGVTSLPP TGCLPAARTL FGFHEKGCVS RLNTDAQNFN KKLNAAASKL QKQYSDLKIV VFDIYSPLYD LVQNPSKSGF TEATKGCCGT
301: GTVETTSLLC NPKSFGTCSN ATQYVFWDSV HPSEAANEIL ATALIGQGFS LLG

Arabidopsis Description

APGGDSL esterase/lipase APG [Source:UniProtKB/Swiss-Prot;Acc:Q9LU14]
SUBAcon: [endoplasmic reticulum,vacuole]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.