CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

nucleus 5
mitochondrion 1
cytosol 1

Predictors

GFP

MS/MS

Papers

endoplasmic reticulum: 27224218
nucleus: 27291164
endoplasmic reticulum: 28499913

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27291164 doi

X Yin, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 27224218 doi

X Wang, S Komatsu

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG93223
KRH13056

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G62190.1	KRG93223	AT1G53720.1	16497658
AT5G62190.1	KRH13056	AT1G53720.1	16497658

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRG91400	Soybean	nucleus	92.11	92.64
GSMUA_Achr7P05930_001	Banana	nucleus	59.97	68.86
VIT_16s0022g02110.t01	Wine grape	plastid	68.01	68.3
PGSC0003DMT400079640	Potato	nucleus	65.85	68.1
Solyc03g007010.2.1	Tomato	nucleus	66.28	67.74
CDX87236	Canola	nucleus	60.55	66.25
PGSC0003DMT400071859	Potato	cytosol, nucleus, plastid	63.41	66.07
CDY16789	Canola	nucleus	59.83	65.67
Bra035903.1-P	Field mustard	nucleus	59.11	65.09
Solyc06g035450.2.1	Tomato	nucleus	36.87	64.09
AT5G62190.1	Thale cress	nucleus	61.69	64.08
Bra029275.1-P	Field mustard	nucleus	60.55	64.04
HORVU5Hr1G080170.2	Barley	nucleus	59.4	63.89
Zm00001d015590_P001	Maize	endoplasmic reticulum, golgi	22.67	63.2
CDY28363	Canola	plastid	24.53	62.41
GSMUA_Achr4P19590_001	Banana	nucleus	63.13	61.11
Os09t0520700-01	Rice	nucleus, plastid	60.11	60.2
Zm00001d006160_P001	Maize	nucleus	60.69	60.09
TraesCS5D01G315900.1	Wheat	mitochondrion, nucleus	60.26	60.0
TraesCS5B01G309500.1	Wheat	nucleus	60.11	59.86
TraesCS5A01G308900.1	Wheat	nucleus	59.83	59.66
EER97062	Sorghum	nucleus	60.69	59.49
Zm00001d021196_P004	Maize	nucleus	60.11	59.26
Zm00001d035226_P001	Maize	plasma membrane	19.51	39.08
Zm00001d035797_P001	Maize	mitochondrion	29.99	36.67
Zm00001d049075_P001	Maize	extracellular, golgi	25.25	36.36
KRH14557	Soybean	mitochondrion	16.07	34.67
Zm00001d050438_P002	Maize	extracellular	15.93	34.47
Zm00001d024138_P001	Maize	plastid	13.92	34.4
Zm00001d049254_P001	Maize	cytosol	9.33	34.03
Zm00001d046048_P001	Maize	cytosol	9.04	33.51
KRH33703	Soybean	plastid	34.43	32.61
KRG90413	Soybean	plastid	34.29	32.47
CDY34116	Canola	cytosol	23.67	32.35
KRH73571	Soybean	nucleus	27.12	31.76
KRH68372	Soybean	nucleus	34.72	31.39
KRG96641	Soybean	nucleus	34.72	31.39
KRH45952	Soybean	mitochondrion	27.12	31.24
KRG99044	Soybean	mitochondrion	27.26	29.55
KRH14556	Soybean	cytosol	11.62	28.22
KRH15837	Soybean	nucleus	6.31	23.53
KRH42587	Soybean	nucleus	10.04	22.8
KRH15843	Soybean	cytosol	10.9	20.05
CDY44622	Canola	cytosol	24.39	19.68
KRH15846	Soybean	nucleus	14.49	18.1
KRH44787	Soybean	nucleus	15.35	17.34

Protein Annotations

EntrezGene:100805921	Gene3D:3.30.70.2280	Gene3D:3.40.50.300	MapMan:35.1	EMBL:ACUP02006674	InterPro:DEAD/DEAH_box_helicase_dom
EnsemblPlantsGene:GLYMA_10G241900	GO:GO:0000166	GO:GO:0003674	GO:GO:0003676	GO:GO:0003723	GO:GO:0003824
GO:GO:0004004	GO:GO:0004386	GO:GO:0005488	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005634	GO:GO:0005730	GO:GO:0005737	GO:GO:0006139	GO:GO:0008150
GO:GO:0008152	GO:GO:0009987	GO:GO:0010501	GO:GO:0016787	InterPro:GUCT	InterPro:Helicase_ATP-bd
InterPro:Helicase_C	UniProt:I1LDY0	InterPro:IPR001650	InterPro:IPR014001	InterPro:IPR014014	EnsemblPlants:KRH35418
ProteinID:KRH35418	ProteinID:KRH35418.1	InterPro:P-loop_NTPase	PFAM:PF00270	PFAM:PF00271	PFAM:PF08152
ScanProsite:PS00039	PFscan:PS51192	PFscan:PS51194	PFscan:PS51195	PANTHER:PTHR24031	PANTHER:PTHR24031:SF237
InterPro:RBD_domain_sf	InterPro:RNA-helicase_DEAD-box_CS	InterPro:RNA_helicase_DEAD_Q_motif	SMART:SM00487	SMART:SM00490	SUPFAM:SSF52540
SUPFAM:SSF54928	UniParc:UPI000233C7F0	SEG:seg	:	:	:

Description

hypothetical protein

Coordinates

chr10:-:47038464..47043327

Molecular Weight (calculated)

75788.8 Da

IEP (calculated)

9.128

GRAVY (calculated)

-0.430

Length

697 amino acids

Sequence
(BLAST)

001: MPSLSLSETD TTTPKPISNK KKPKTQSLTD PDLDGVSGKK TKKRKASDLL EPEAMPAAYS YNNGDDETSS DLVEPEPASR EDDSQNKKKK KKKVVKSEEK
101: EQPLLVTEPK EEKKDDPNAI SNFRISEPLR EKLKEKGIES LFPIQAMTFD TVLDGSDLVG RARTGQGKTL AFVLPILESL INGPAKSARK TGYGRTPSVL
201: VLLPTRELAC QVHADFEVYG GAMGLSSCCL YGGAPYQGQE LKLRRGVDIV IGTPGRVKDH IEKGNIDLSQ LKFRVLDEAD EMLRMGFVED VEMILGKVEN
301: VNKVQTLLFS ATLPDWVKQI ALKFLKPDKK TADLVGNTKM KASTNVRHIV LPCTSSARAQ LIPDIIRCYS SGGRTIVFTE TKECASQLAG ILNGAKALHG
401: DIQQSTREVT LSGFRSGKFM TLVATNVAAR GLDINDVQLI IQCEPPRDVE AYIHRSGRTG RAGNTGVAVM LYDPKRSNIP RIERESGVKF EHVSAPQPDD
501: IAKAVSGEAA EMIIQVSDSV VPAFKSAAEE LLNSSGLPVI ELLAKALAKA VGYTDVKQRS LLTSMENYVT LVLETGKPIY TQSYGYSILR RFLPEEKVEA
601: VKGLSLTADG NGVVFDVPAK DLDIYLNGQE NASNVCLEVV KTLPQLQQKE PQSRGGRFGD NGGRGGNYRF GGRGGGGRNG RFSNGGGRGG NWGGKRW

Best Arabidopsis Sequence Match ( AT5G62190.1 )
(BLAST)

001: MPSLMLSDKK EEKKMKKKMA LDTPELDSKK GKKEQKLKLS DSDEEESEKK KSKKKDKKRK ASEEEDEVKS DSSSEKKKSS KKVKLGVEDV EVDNPNAVSK
101: FRISAPLREK LKANGIEALF PIQASTFDMV LDGADLVGRA RTGQGKTLAF VLPILESLVN GPAKSKRKMG YGRSPSVLVL LPTRELAKQV AADFDAYGGS
201: LGLSSCCLYG GDSYPVQEGK LKRGVDIVVG TPGRIKDHIE RQNLDFSYLQ FRVLDEADEM LRMGFVEDVE LILGKVEDST KVQTLLFSAT LPSWVKNISN
301: RFLKRDQKTI DLVGNDKMKA SNSVRHIAIP CNKAAMARLI PDIISCYSSG GQTIIFAETK VQVSELSGLL DGSRALHGEI PQSQREVTLA GFRNGKFATL
401: VATNVAARGL DINDVQLIIQ CEPPREVEAY IHRSGRTGRA GNTGVAVTLY DSRKSSVSRI EKEAGIKFEH LAAPQPDEIA RSGGMEAAEK VKQVCDSVVP
501: AFLEAAKELL ETSGLSAEVL LAKALAKTAG FTEIKKRSLL TSMENYVTLH LEAGKPIYSP SFVYGLLRRV LPDDKVEMIE GLSLTADKTG AVFDVKQSDL
601: DLFIAGAQKS AGSMSLEVVK VMPKLQEREP LPQKRFGGGG RGNRFGGGGG NRFGGGGGRG RGGSGGRGQR Y

Arabidopsis Description

RH7DEAD-box ATP-dependent RNA helicase 7 [Source:UniProtKB/Swiss-Prot;Acc:Q39189]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.