CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, nucleus

Predictor Summary:

plastid 1
mitochondrion 3
cytosol 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	endoplasmic reticulum, nucleus
Any Predictor:	cytosol, mitochondrion
BaCelLo:	plastid
iPSORT:	mitochondrion
MultiLoc:	cytosol
PProwler:	mitochondrion
WoLF PSORT:	mitochondrion
YLoc:	cytosol

nucleus: 21132161
endoplasmic reticulum: 27224218
nucleus: 27291164
endoplasmic reticulum: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27291164 doi

X Yin, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 27224218 doi

X Wang, S Komatsu

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH22291
KRH27037

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G74050.1	KRH22291	AT2G42580.1	19000166
AT1G74050.1	KRH27037	AT2G42580.1	19000166

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH43314	Soybean	nucleus	94.37	93.97
KRH59448	Soybean	endoplasmic reticulum, nucleus	93.07	92.67
KRH59450	Soybean	endoplasmic reticulum	96.1	91.74
KRH43467	Soybean	nucleus	89.18	88.79
KRH13901	Soybean	nucleus	88.74	88.36
VIT_13s0064g01390.t01	Wine grape	cytosol	83.98	83.26
CDY66361	Canola	cytosol	80.95	80.26
Bra008128.1-P	Field mustard	cytosol	80.95	80.26
CDX81879	Canola	extracellular	80.52	79.49
Bra015940.1-P	Field mustard	cytosol	80.09	79.4
Bra031034.1-P	Field mustard	cytosol	80.09	79.06
CDY26150	Canola	cytosol	80.09	79.06
CDX96510	Canola	cytosol	79.65	78.97
CDY45859	Canola	cytosol	79.22	78.88
CDY11078	Canola	cytosol	70.13	78.64
CDY21701	Canola	cytosol	79.65	78.63
Solyc04g014720.2.1	Tomato	nucleus	77.92	78.6
Solyc05g054070.2.1	Tomato	nucleus	77.92	78.6
AT1G18540.1	Thale cress	cytosol	79.22	78.54
AT1G74060.1	Thale cress	cytosol	79.22	78.54
Bra025895.1-P	Field mustard	cytosol	79.22	78.21
PGSC0003DMT400060697	Potato	cytosol	77.49	78.17
PGSC0003DMT400034524	Potato	cytosol	77.49	78.17
AT1G74050.1	Thale cress	cytosol	78.79	78.11
GSMUA_Achr9P22280_001	Banana	cytosol	69.7	77.78
CDY19222	Canola	cytosol	78.79	77.78
CDX96716	Canola	cytosol	78.79	77.78
Solyc11g012110.1.1	Tomato	nucleus	77.06	77.73
CDX83840	Canola	cytosol	78.36	77.35
PGSC0003DMT400021753	Potato	cytosol	76.62	77.29
GSMUA_Achr3P28460_001	Banana	cytosol	68.83	76.81
Bra016555.1-P	Field mustard	cytosol	77.49	76.5
CDX73042	Canola	cytosol	77.06	76.39
GSMUA_Achr4P06390_001	Banana	mitochondrion	77.06	76.07
Zm00001d049263_P001	Maize	cytosol	21.21	71.01
EES12351	Sorghum	mitochondrion	65.8	69.41
Zm00001d050920_P002	Maize	plastid	64.94	68.49
Zm00001d050921_P002	Maize	plastid	64.5	68.04
EES07070	Sorghum	mitochondrion	64.5	68.04
Os04t0473400-01	Rice	nucleus, plastid	65.37	68.02
Zm00001d017047_P001	Maize	plastid	64.07	67.58
Os02t0591700-01	Rice	nucleus	62.34	65.75
TraesCS6A01G204900.1	Wheat	mitochondrion	61.47	64.84
TraesCS6B01G225600.1	Wheat	mitochondrion	61.47	64.84
TraesCS6D01G190100.1	Wheat	nucleus	61.47	64.84
HORVU6Hr1G041750.1	Barley	mitochondrion	59.74	57.98
TraesCS6D01G180100.1	Wheat	mitochondrion	61.47	57.26
TraesCS6B01G230300.2	Wheat	mitochondrion	60.17	56.05
HORVU6Hr1G052600.3	Barley	plastid	60.17	53.26
Zm00001d025591_P001	Maize	cytosol	41.56	42.48
Zm00001d041756_P001	Maize	plastid	41.99	22.99

Protein Annotations

EntrezGene:100500022	MapMan:17.1.2.1.4	Gene3D:2.30.30.30	InterPro:60S_ribosomal_L6E	EMBL:ACUP02004851	EMBL:BT090560
UniProt:C6SZF9	EnsemblPlantsGene:GLYMA_08G141800	GO:GO:0000027	GO:GO:0002181	GO:GO:0003674	GO:GO:0003676
GO:GO:0003723	GO:GO:0003735	GO:GO:0005198	GO:GO:0005488	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0005829	GO:GO:0005840	GO:GO:0006412	GO:GO:0008150
GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0016043	GO:GO:0019538	GO:GO:0022625
InterPro:IPR014722	EnsemblPlants:KRH43315	ProteinID:KRH43315	ProteinID:KRH43315.1	PFAM:PF01159	PFAM:PF03868
PANTHER:PTHR10715	InterPro:Rib_L2_dom2	InterPro:Ribosomal_L6_N	SUPFAM:SSF50104	InterPro:Translation_prot_SH3-like_sf	UniParc:UPI0001B1502F
SEG:seg	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr8:+:10822917..10824849

Molecular Weight (calculated)

25762.7 Da

IEP (calculated)

10.921

GRAVY (calculated)

-0.585

Length

231 amino acids

Sequence
(BLAST)

001: MAPKITRKVS RNPELIRGIG KYSRSQMYHK RGIWAIKAKN GGVLPRHGPK PKPETPAEKP PKFYPADDVK KPLVNKHKPK PAKLRASITP GTVLILLAGR
101: FKGKRVVFLK QLPSGLLLVT GPFKINGVPL RRVNQSYVIG TSTKANVSAV NVEQFDDKYF TKEAQKKKKG EGEFFEAEKE EKSVLPQQKK DDQKTVDSAL
201: IKAIESVPDL KTYLGARFSL KAGVKPHELV F

Best Arabidopsis Sequence Match ( AT1G74060.1 )
(BLAST)

001: MPAKQRTAKV NRNPDLIRGV GKYSRSQMYH KRGLWAIKAK NGGVFPRHDA KSKVDAPVEK PPKFYPAEDV KKPLPNRRTA KPAKLRASIT PGTVLIILAG
101: RFKGKRVVFL KQLASGLLLV TGPFKINGVP LRRVNQAYVI GTSTKVDISG VTLDKFDDKY FGKVAEKKKK KTEGEFFEAE KEEKKEIPQG KKDDQKAVDA
201: ALIKAIEAVP ELKTYLGARF SLKQGMKPHE LVF

Arabidopsis Description

RPL6B60S ribosomal protein L6-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9C9C6]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.