CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, nucleus

Predictor Summary:

plastid 2
cytosol 3
mitochondrion 3

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH22291
KRH27037

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G74050.1	KRH22291	AT2G42580.1	19000166
AT1G74050.1	KRH27037	AT2G42580.1	19000166

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH43314	Soybean	nucleus	96.12	96.12
KRH43315	Soybean	endoplasmic reticulum, nucleus	92.67	93.07
KRH43467	Soybean	nucleus	90.52	90.52
KRH59450	Soybean	endoplasmic reticulum	94.4	90.5
KRH13901	Soybean	nucleus	90.09	90.09
VIT_13s0064g01390.t01	Wine grape	cytosol	83.62	83.26
PGSC0003DMT400034524	Potato	cytosol	78.45	79.48
AT1G74060.1	Thale cress	cytosol	79.74	79.4
Solyc11g012110.1.1	Tomato	nucleus	78.02	79.04
AT1G74050.1	Thale cress	cytosol	79.31	78.97
Bra015940.1-P	Field mustard	cytosol	79.31	78.97
CDX96510	Canola	cytosol	78.88	78.54
AT1G18540.1	Thale cress	cytosol	78.88	78.54
Solyc04g014720.2.1	Tomato	nucleus	77.16	78.17
Solyc05g054070.2.1	Tomato	nucleus	77.16	78.17
CDY26150	Canola	cytosol	78.45	77.78
Bra031034.1-P	Field mustard	cytosol	78.45	77.78
PGSC0003DMT400060697	Potato	cytosol	76.72	77.73
PGSC0003DMT400021753	Potato	cytosol	76.72	77.73
CDY66361	Canola	cytosol	78.02	77.68
Bra008128.1-P	Field mustard	cytosol	78.02	77.68
CDY11078	Canola	cytosol	68.97	77.67
CDX81879	Canola	extracellular	78.02	77.35
CDY21701	Canola	cytosol	78.02	77.35
GSMUA_Achr3P28460_001	Banana	cytosol	68.97	77.29
CDY19222	Canola	cytosol	77.59	76.92
Bra025895.1-P	Field mustard	cytosol	77.59	76.92
CDX96716	Canola	cytosol	77.59	76.92
GSMUA_Achr9P22280_001	Banana	cytosol	68.53	76.81
CDX83840	Canola	cytosol	77.16	76.5
CDY45859	Canola	cytosol	76.29	76.29
Bra016555.1-P	Field mustard	cytosol	76.72	76.07
GSMUA_Achr4P06390_001	Banana	mitochondrion	76.29	75.64
CDX73042	Canola	cytosol	75.86	75.54
Zm00001d049263_P001	Maize	cytosol	21.12	71.01
EES12351	Sorghum	mitochondrion	64.22	68.04
Zm00001d050920_P002	Maize	plastid	63.79	67.58
Zm00001d017047_P001	Maize	plastid	63.79	67.58
EES07070	Sorghum	mitochondrion	63.79	67.58
Zm00001d050921_P002	Maize	plastid	63.36	67.12
Os04t0473400-01	Rice	nucleus, plastid	63.79	66.67
Os02t0591700-01	Rice	nucleus	62.93	66.67
TraesCS6B01G225600.1	Wheat	mitochondrion	61.21	64.84
TraesCS6D01G190100.1	Wheat	nucleus	61.21	64.84
TraesCS6A01G204900.1	Wheat	mitochondrion	61.21	64.84
HORVU6Hr1G041750.1	Barley	mitochondrion	59.48	57.98
TraesCS6D01G180100.1	Wheat	mitochondrion	61.64	57.66
TraesCS6B01G230300.2	Wheat	mitochondrion	60.78	56.85
HORVU6Hr1G052600.3	Barley	plastid	59.91	53.26
Zm00001d025591_P001	Maize	cytosol	41.38	42.48
Zm00001d041756_P001	Maize	plastid	41.81	22.99

Protein Annotations

EntrezGene:100809343	MapMan:17.1.2.1.4	Gene3D:2.30.30.30	InterPro:60S_ribosomal_L6E	EMBL:ACUP02003324	ncoils:Coil
EnsemblPlantsGene:GLYMA_05G184000	GO:GO:0003674	GO:GO:0003735	GO:GO:0005198	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0005840	GO:GO:0006412	GO:GO:0008150	GO:GO:0008152
GO:GO:0009058	GO:GO:0009987	GO:GO:0019538	UniProt:I1K4Q5	InterPro:IPR014722	EnsemblPlants:KRH59448
ProteinID:KRH59448	ProteinID:KRH59448.1	PFAM:PF01159	PFAM:PF03868	PANTHER:PTHR10715	InterPro:Rib_L2_dom2
InterPro:Ribosomal_L6_N	SUPFAM:SSF50104	InterPro:Translation_prot_SH3-like_sf	UniParc:UPI0002339F7D	SEG:seg	:

Description

hypothetical protein

Coordinates

chr5:+:37118518..37120547

Molecular Weight (calculated)

25774.7 Da

IEP (calculated)

10.896

GRAVY (calculated)

-0.546

Length

232 amino acids

Sequence
(BLAST)

001: MAPKVTRKVS RNPELIRGIG KYSRSQMYHK RGIWAIKAKN GGVLPRHDPK PKPETPAEKP PKFYPADDIK KPLVNKHKPK PAKLRASITP GTVLILLAGR
101: FKGKRVVFLK QLPSGLLLVT GPFKVNGVPL RRVNQAYVIG TSTKVDISAV NVDSFGDKYF AKEAQKKKKK GEGEFFEAEK EEKSVLPQQK KDDQKTVDAA
201: VLKAIESVPD LKSYLGARFS LKAGVKPHEL VF

Best Arabidopsis Sequence Match ( AT1G18540.1 )
(BLAST)

001: MPAAKRTPKV NRNPDLIRGV GKYSRSQMYH KRGLWAIKAK NGGVFPRHDA QPKVDAPVEK PAKFYPAEDV KKPLVNRRKP KPTKLKASIT PGTVLIILAG
101: RFKGKRVVFL KQLSSGLLLV TGPFKINGVP LRRVNQAYVI GTSTKIDISG VNTEKFDDKY FGKVAEKKKK KTEGEFFEAE KEEKKEIPQE KKEDQKTVDA
201: ALIKSIEAVP ELKVYLGARF SLSQGMKPHE LVF

Arabidopsis Description

RPL6A60S ribosomal protein L6-1 [Source:UniProtKB/Swiss-Prot;Acc:Q9FZ76]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.