Factsheet for Solyc12g011000.1.1

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

plastid 5
mitochondrion 3
peroxisome 1
cytosol 1
extracellular 1
endoplasmic reticulum 1
vacuole 1
plasma membrane 1
golgi 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, mitochondrion, peroxisome, plastid, secretory
BaCelLo:	plastid
ChloroP:	plastid
EpiLoc:	plastid
iPSORT:	mitochondrion
MultiLoc:	mitochondrion
Plant-mPloc:	cytosol, peroxisome
PProwler:	mitochondrion
TargetP:	secretory
WoLF PSORT:	plastid
YLoc:	plastid

nucleus: 25464976
plastid: 26371478
unclear: 26455813
nucleus: 28394025

msms PMID: 28394025 doi

AJM Howden, R Stam, V Martinez Heredia, GB Motion, S Ten Have, K Hodge, TM Marques Monteiro Amaro, E Huitema

Cell and Molecular Sciences, JHI, Invergowrie, Dundee, DD2 5DA, UK., Division of Plant Science, School of Life Sciences, University of Dundee at the James Hutton Institute (JHI), Invergowrie, Dundee, DD2 5DA, UK., Dundee Effector Consortium, JHI, Invergowrie, Dundee, DD2 5DA, UK., Section of Population Genetics, Technische Universität München, 85354, Freising, Germany., Wellcome Trust Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, UK.

msms PMID: 26371478 doi

M Suzuki, S Takahashi, T Kondo, H Dohra, Y Ito, Y Kiriiwa, M Hayashi, S Kamiya, M Kato, M Fujiwara, Y Fukao, M Kobayashi, N Nagata, R Motohashi

Faculty of Agriculture, Shizuoka University, Shizuoka city, Shizuoka, Japan., Faculty of Science, Japan Woman's University, Bunkyo-ku, Tokyo, Japan., Instrumental Research Support Office, Research Institute of Green Science and Technology, Shizuoka University, Shizuoka city, Shizuoka, Japan., The Plant Science Education Unit, Nara Institute of Science and Technology, Ikoma city, Nara, Japan.

msms PMID: 26455813 doi

P Paul, P Chaturvedi, M Selymesi, A Ghatak, A Mesihovic, KD Scharf, W Weckwerth, S Simm, E Schleiff

Goethe University, Department of Biosciences, Molecular Cell Biology of Plants, Frankfurt/Main, Germany., Goethe University, Department of Biosciences, Molecular Cell Biology of Plants, Frankfurt/Main, Germany; SPOT-ITN Consortium., Goethe University, Department of Biosciences, Molecular Cell Biology of Plants, Frankfurt/Main, Germany; SPOT-ITN Consortium; Goethe University, Cluster of Excellence Frankfurt, Frankfurt/Main, Germany; Goethe University, Buchmann Institute of Molecular Life Sciences, Frankfurt/Main, Germany. Electronic address: schleiff@bio.uni-frankfurt.de., SPOT-ITN Consortium; University of Vienna, Department of Ecogenomics and Systems biology, Vienna, Austria., University of Vienna, Department of Ecogenomics and Systems biology, Vienna, Austria; School of Biotechnology and Bioinformatics, D.Y. Patil University, Mumbai, India.

msms PMID: 25464976 doi

Y Wang, W Wang, J Cai, Y Zhang, G Qin, S Tian

PPI

Inferred distinct locusB in Crop

locusB	locations
Solyc01g104680.2.1
Solyc01g104700.2.1
Solyc03g120690.2.1
Solyc04g007230.2.1
Solyc05g023800.2.1
Solyc05g055210.1.1
Solyc07g006130.1.1
Solyc07g055840.2.1
Solyc08g036600.1.1
Solyc08g076410.2.1
Solyc11g006660.1.1
Solyc12g010520.1.1
Solyc12g011000.1.1
Solyc12g044240.1.1
Solyc12g094450.1.1

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G42790.1	Solyc05g055210.1.1	AT1G12920.1	21798944
AT2G42790.1	Solyc07g006130.1.1	AT1G12920.1	21798944
AT2G42790.1	Solyc11g006660.1.1	AT1G12920.1	21798944
AT2G42790.1	Solyc12g010520.1.1	AT1G12920.1	21798944
AT2G42790.1	Solyc12g094450.1.1	AT1G76150.1	21798944
AT2G42790.1	Solyc08g076410.2.1	AT2G35500.1	21798944
AT2G42790.1	Solyc03g120690.2.1	AT3G16120.1	21798944
AT2G42790.1	Solyc12g044240.1.1	AT3G16120.1	21798944
AT2G42790.1	Solyc08g036600.1.1	AT3G56900.1	21798944
AT2G42790.1	Solyc07g055840.2.1	AT3G58750.1	21798944
AT2G42790.1	Solyc12g011000.1.1	AT3G58750.1	21798944
AT2G42790.1	Solyc01g104680.2.1	AT5G55190.1	21798944
AT2G42790.1	Solyc01g104700.2.1	AT5G55190.1	21798944
AT2G42790.1	Solyc05g023800.2.1	AT5G55190.1	21798944
AT3G58740.1	Solyc07g055840.2.1	AT2G42790.1	21798944
AT3G58740.1	Solyc12g011000.1.1	AT2G42790.1	21798944
AT3G58750.1	Solyc04g007230.2.1	AT1G25420.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
PGSC0003DMT400020128	Potato	plastid	98.44	98.44
CDX79848	Canola	cytosol	37.94	88.24
Solyc07g055840.2.1	Tomato	nucleus, unclear	86.58	87.08
CDY15651	Canola	nucleus	29.77	85.96
VIT_12s0142g00610.t01	Wine grape	plastid	84.24	84.57
CDY49252	Canola	cytosol	81.71	82.84
CDX98329	Canola	cytosol, peroxisome, plastid	81.71	82.84
CDX67764	Canola	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, peroxisome, plasma membrane, vacuole	81.71	82.68
Bra007417.1-P	Field mustard	cytosol	81.52	82.64
Bra003355.1-P	Field mustard	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, peroxisome, plasma membrane, vacuole	81.52	82.48
CDX71925	Canola	cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	81.71	82.19
CDY50821	Canola	cytosol	81.32	81.96
AT2G42790.1	Thale cress	cytosol	81.13	81.93
AT3G58750.1	Thale cress	endoplasmic reticulum, extracellular, golgi, mitochondrion, plasma membrane, vacuole	81.91	81.91
Bra004720.1-P	Field mustard	cytosol	81.13	81.76
CDX79851	Canola	cytosol	81.13	81.76
GSMUA_Achr10P...	Banana	mitochondrion	74.32	80.25
Os02t0232400-01	Rice	nucleus, plastid	77.63	79.8
EES04804	Sorghum	mitochondrion	78.21	79.6
TraesCS5B01G416700.1	Wheat	golgi	77.82	79.52
Zm00001d016248_P001	Maize	mitochondrion	77.82	79.52
TraesCS5D01G422000.1	Wheat	unclear	77.63	79.32
TraesCS5A01G413300.1	Wheat	mitochondrion	77.63	79.32
Bra007416.1-P	Field mustard	cytosol	67.7	77.51
CDY49249	Canola	cytosol	70.82	77.45
HORVU5Hr1G099820.5	Barley	plastid	77.04	77.34
KRH14452	Soybean	nucleus	76.65	77.1
KRH46090	Soybean	endoplasmic reticulum, nucleus	75.68	75.83
KRH73702	Soybean	cytosol, plastid	75.29	75.59
CDY00587	Canola	cytosol	68.87	75.32
KRG98876	Soybean	nucleus, plastid	75.29	75.29
Bra014554.1-P	Field mustard	cytosol	68.48	74.89
AT3G58740.1	Thale cress	cytosol	67.9	72.71
CDX71926	Canola	cytosol	65.95	70.77
CDX89002	Canola	endoplasmic reticulum, golgi	34.82	63.25
HORVU0Hr1G030280.2	Barley	cytosol	19.84	56.67
CDY49250	Canola	extracellular	8.37	53.09
VIT_06s0080g00770.t01	Wine grape	cytosol	21.01	50.94
CDX71928	Canola	cytosol	69.84	43.41
Solyc01g073740.2.1	Tomato	plastid	20.04	21.73

Protein Annotations

Gene3D:1.10.230.10	Gene3D:1.10.580.10	MapMan:5.7.3.6.1	InterPro:Citrate_synth-like_lrg_a-sub	InterPro:Citrate_synth-like_sm_a-sub	InterPro:Citrate_synthase
InterPro:Citrate_synthase_AS	InterPro:Citrate_synthase_bac-typ	InterPro:Citrate_synthase_sf	GO:GO:0003674	GO:GO:0003824	GO:GO:0006091
GO:GO:0006099	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0016740	GO:GO:0046912
InterPro:IPR016142	InterPro:IPR016143	UniProt:K4DCI6	PFAM:PF00285	PIRSF:PIRSF001369	PRINTS:PR00143
ScanProsite:PS00480	PANTHER:PTHR11739	PANTHER:PTHR11739:SF4	SUPFAM:SSF48256	EnsemblPlantsGene:Solyc12g011000.1	EnsemblPlants:Solyc12g011000.1.1
UniParc:UPI0002769FFF	SEG:seg	:	:	:	:

Description

Citrate synthase [Source:UniProtKB/TrEMBL;Acc:K4DCI6]

Coordinates

chr12:-:3858049..3864969

Molecular Weight (calculated)

56346.7 Da

IEP (calculated)

8.932

GRAVY (calculated)

-0.167

Length

514 amino acids

Sequence
(BLAST)

001: MENGNSSSVA RGRLAVLSAH LAASLNVSDL TTFKLLETSG VSSVSGVEPP RNLKGALTII DERTGKKYPV QVSEDGTIKA NDLKKITTGQ NDKGLKLYDP
101: GYLNTAPVRS SICYIDGDAG ILRYRGYPIE ELAEGSSFLE VAYLLLYGNL PSENQLADWE FTVSQHSAVP QGLLDIIQSM PHDAHPMGVL VSAMSALSVF
201: HPDANPALRG QDIYKSKQVR DKQIVRILGK APTIATAAYL RMAGRPPVLP SNNLSYAENF LYMLDSLGNR SYKPNPRLAR VLDILFILHA EHEMNCSTAA
301: ARHLASSGVD VYTAIAGAVG ALYGPLHGGA NEAVLKMLSE IGSVENIPEF LEGVKNRKRK MSGFGHRVYK NYDPRAKVIK TLADEVFSIV GRDPLIEVAV
401: ALEKAALSDE YFVKRKLYPN VDFYSGLIYR AMGFPTEFFP VLFAIPRMAG YLSHWNESLD DPDTKIMRPA QVYTGVWMRH YMPLKERSPH SEADKLGHVS
501: VSNATKRRLA GSGA

Best Arabidopsis Sequence Match ( AT3G58750.1 )
(BLAST)

001: MEISQRVKAR LAVLTAHLAV SDTVGLEQVL PAIAPWCTSA HITAAPHGSL KGNLTIVDER TGKKYQVPVS EHGTVKAVDL KKITTGKDDK GLKLYDPGYL
101: NTAPVRSSIC YIDGDEGILR YRGYPIEELA ESSTFIEVAY LLMYGNLPSQ SQLADWEFTV SQHSAVPQGV LDIIQSMPHD AHPMGVLVSA MSALSIFHPD
201: ANPALSGQDI YKSKQVRDKQ IVRILGKAPT IAAAAYLRTA GRPPVLPSAN LSYSENFLYM LDSMGNRSYK PNPRLARVLD ILFILHAEHE MNCSTAAARH
301: LASSGVDVYT ACAGAVGALY GPLHGGANEA VLKMLAEIGT AENIPDFIEG VKNRKRKMSG FGHRVYKNYD PRAKVIKKLA DEVFSIVGRD PLIEVAVALE
401: KAALSDEYFV KRKLYPNVDF YSGLIYRAMG FPPEFFTVLF AVPRMAGYLS HWRESLDDPD TRIMRPQQAY TGVWMRHYEP VRERTLSSDS DKDKFGQVSI
501: SNASRRRLAG SSAL

Arabidopsis Description

CSY2Citrate synthase 2, peroxisomal [Source:UniProtKB/Swiss-Prot;Acc:Q9LXS6]
SUBAcon: [extracellular,endoplasmic reticulum,vacuole,mitochondrion,plasma membrane,golgi]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.