CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, nucleus

Predictor Summary:

plastid 3
vacuole 1
mitochondrion 4
peroxisome 1
cytosol 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG90825
KRG94758
KRG95023
KRG98876
KRH06769
KRH08442
KRH09439
KRH09878
KRH14452
KRH23441
KRH35947
KRH37825
KRH38906
KRH46090
KRH49412
KRH51640
KRH52483
KRH52484
KRH52485
KRH60966
KRH61808
KRH61809
KRH61811
KRH66657
KRH70101
KRH73702
KRH74369

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G42790.1	KRG94758	AT1G12920.1	21798944
AT2G42790.1	KRH06769	AT1G12920.1	21798944
AT2G42790.1	KRH08442	AT1G12920.1	21798944
AT2G42790.1	KRH09439	AT1G12920.1	21798944
AT2G42790.1	KRH38906	AT1G12920.1	21798944
AT2G42790.1	KRH70101	AT1G12920.1	21798944
AT2G42790.1	KRH51640	AT1G76150.1	21798944
AT2G42790.1	KRH60966	AT1G76150.1	21798944
AT2G42790.1	KRH49412	AT2G35500.1	21798944
AT2G42790.1	KRH74369	AT2G35500.1	21798944
AT2G42790.1	KRH09878	AT3G16120.1	21798944
AT2G42790.1	KRH23441	AT3G16120.1	21798944
AT2G42790.1	KRG95023	AT3G56900.1	21798944
AT2G42790.1	KRH37825	AT3G56900.1	21798944
AT2G42790.1	KRH66657	AT3G56900.1	21798944
AT2G42790.1	KRG98876	AT3G58750.1	21798944
AT2G42790.1	KRH14452	AT3G58750.1	21798944
AT2G42790.1	KRH46090	AT3G58750.1	21798944
AT2G42790.1	KRH73702	AT3G58750.1	21798944
AT2G42790.1	KRH52483	AT5G55190.1	21798944
AT2G42790.1	KRH52484	AT5G55190.1	21798944
AT2G42790.1	KRH52485	AT5G55190.1	21798944
AT2G42790.1	KRH61808	AT5G55190.1	21798944
AT2G42790.1	KRH61809	AT5G55190.1	21798944
AT2G42790.1	KRH61811	AT5G55190.1	21798944
AT3G58740.1	KRG98876	AT2G42790.1	21798944
AT3G58740.1	KRH14452	AT2G42790.1	21798944
AT3G58740.1	KRH46090	AT2G42790.1	21798944
AT3G58740.1	KRH73702	AT2G42790.1	21798944
AT3G58750.1	KRG90825	AT1G25420.1	21798944
AT3G58750.1	KRH35947	AT1G25420.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRG98876	Soybean	nucleus, plastid	95.52	95.33
KRH73702	Soybean	cytosol, plastid	84.21	84.38
KRH14452	Soybean	nucleus	83.24	83.56
CDX79848	Canola	cytosol	35.28	81.9
CDY15651	Canola	nucleus	27.68	79.78
VIT_12s0142g00610.t01	Wine grape	plastid	76.41	76.56
AT2G42790.1	Thale cress	cytosol	75.24	75.83
Solyc07g055840.2.1	Tomato	nucleus, unclear	75.44	75.73
CDY50821	Canola	cytosol	75.24	75.69
Solyc12g011000.1.1	Tomato	nucleus	75.83	75.68
Bra007417.1-P	Field mustard	cytosol	74.66	75.54
CDX79851	Canola	cytosol	75.05	75.49
Bra004720.1-P	Field mustard	cytosol	75.05	75.49
PGSC0003DMT400020128	Potato	plastid	75.63	75.49
CDY49252	Canola	cytosol	74.46	75.35
AT3G58750.1	Thale cress	endoplasmic reticulum, extracellular, golgi, mitochondrion, plasma membrane, vacuole	75.44	75.29
GSMUA_Achr10P...	Banana	mitochondrion	69.79	75.21
CDX67764	Canola	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, peroxisome, plasma membrane, vacuole	74.46	75.2
CDX98329	Canola	cytosol, peroxisome, plastid	74.27	75.15
Bra003355.1-P	Field mustard	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, peroxisome, plasma membrane, vacuole	74.27	75.0
CDX71925	Canola	cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	74.66	74.95
PGSC0003DMT400044692	Potato	plastid	74.46	74.76
TraesCS5A01G413300.1	Wheat	mitochondrion	73.29	74.75
TraesCS5D01G422000.1	Wheat	unclear	73.29	74.75
EES04804	Sorghum	mitochondrion	73.29	74.46
TraesCS5B01G416700.1	Wheat	golgi	72.9	74.35
Zm00001d016248_P001	Maize	mitochondrion	72.51	73.96
Os02t0232400-01	Rice	nucleus, plastid	71.73	73.6
Bra007416.1-P	Field mustard	cytosol	63.55	72.61
CDY49249	Canola	cytosol	66.08	72.13
HORVU5Hr1G099820.5	Barley	plastid	71.93	72.07
Bra014554.1-P	Field mustard	cytosol	64.13	70.0
CDY00587	Canola	cytosol	63.94	69.79
AT3G58740.1	Thale cress	cytosol	64.13	68.54
CDX71926	Canola	cytosol	62.18	66.6
CDX89002	Canola	endoplasmic reticulum, golgi	34.11	61.84
CDY49250	Canola	extracellular	7.99	50.62
HORVU0Hr1G030280.2	Barley	cytosol	17.74	50.56
VIT_06s0080g00770.t01	Wine grape	cytosol	19.69	47.64
CDX71928	Canola	cytosol	65.3	40.51
KRH13859	Soybean	mitochondrion	21.83	23.73
KRH43565	Soybean	mitochondrion	21.83	23.73

Protein Annotations

Gene3D:1.10.230.10	Gene3D:1.10.580.10	EntrezGene:100775180	MapMan:5.7.3.6.1	EMBL:ACUP02005317	InterPro:Citrate_synth-like_lrg_a-sub
InterPro:Citrate_synth-like_sm_a-sub	InterPro:Citrate_synthase	InterPro:Citrate_synthase_AS	InterPro:Citrate_synthase_bac-typ	InterPro:Citrate_synthase_sf	EnsemblPlantsGene:GLYMA_08G311200
GO:GO:0003674	GO:GO:0003824	GO:GO:0006091	GO:GO:0006099	GO:GO:0008150	GO:GO:0008152
GO:GO:0009987	GO:GO:0016740	GO:GO:0046912	UniProt:I1KY39	InterPro:IPR016142	InterPro:IPR016143
EnsemblPlants:KRH46090	ProteinID:KRH46090	ProteinID:KRH46090.1	PFAM:PF00285	PIRSF:PIRSF001369	PRINTS:PR00143
ScanProsite:PS00480	PANTHER:PTHR11739	PANTHER:PTHR11739:SF4	SUPFAM:SSF48256	UniParc:UPI0002338B37	:

Description

hypothetical protein

Coordinates

chr8:+:43045422..43053149

Molecular Weight (calculated)

56482.9 Da

IEP (calculated)

9.592

GRAVY (calculated)

-0.201

Length

513 amino acids

Sequence
(BLAST)

001: MSKTTTSSEQ TARNRLATLA AHLLPSDATS AAAILHPHRL SAPTGDLPPA NLKGTLTVVD ERTGKKYQIE VTPDGTVRAS DFKKISSGKN DKGLKLYDPG
101: YLNTAPVISR ISYIDGDAGI LRYRGYPIED LAEKSTFTEV SYLILYGNLP SESQLAEWEF ALSQHSAVPQ GVLDMIESMP HDAHPMGMLV NAMSALSVFH
201: PDANPALKGL DIYNSKQVRD KQIARVIGKI TTIAAAINLR LAGRPPVLPS NKLSYTENFL YMLDSFGNRS YKPNPRLTRA LDIIFVLHAE HEMNCSTSAV
301: RHLSSSGVDV YTAIAGAVGA LYGPLHGGAN EAVLKMLSEI GTVENIPAFI EGVKARKRKL SGFGHRVYKN YDPRAKVLRK LTEEVFSIVG RDPLIEIAVA
401: LEKIALSDEY FIKRKLYPNV DFYSGLIYRA MGFQPEFFTV LFAIPRMAGY LAHWRESLDD PDTKIMRPQQ VYVGEWLRHY APINQRTASS NTDKLGQLAV
501: SNASKRRLAG SGA

Best Arabidopsis Sequence Match ( AT3G58750.1 )
(BLAST)

001: MEISQRVKAR LAVLTAHLAV SDTVGLEQVL PAIAPWCTSA HITAAPHGSL KGNLTIVDER TGKKYQVPVS EHGTVKAVDL KKITTGKDDK GLKLYDPGYL
101: NTAPVRSSIC YIDGDEGILR YRGYPIEELA ESSTFIEVAY LLMYGNLPSQ SQLADWEFTV SQHSAVPQGV LDIIQSMPHD AHPMGVLVSA MSALSIFHPD
201: ANPALSGQDI YKSKQVRDKQ IVRILGKAPT IAAAAYLRTA GRPPVLPSAN LSYSENFLYM LDSMGNRSYK PNPRLARVLD ILFILHAEHE MNCSTAAARH
301: LASSGVDVYT ACAGAVGALY GPLHGGANEA VLKMLAEIGT AENIPDFIEG VKNRKRKMSG FGHRVYKNYD PRAKVIKKLA DEVFSIVGRD PLIEVAVALE
401: KAALSDEYFV KRKLYPNVDF YSGLIYRAMG FPPEFFTVLF AVPRMAGYLS HWRESLDDPD TRIMRPQQAY TGVWMRHYEP VRERTLSSDS DKDKFGQVSI
501: SNASRRRLAG SSAL

Arabidopsis Description

CSY2Citrate synthase 2, peroxisomal [Source:UniProtKB/Swiss-Prot;Acc:Q9LXS6]
SUBAcon: [extracellular,endoplasmic reticulum,vacuole,mitochondrion,plasma membrane,golgi]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.