Factsheet for Os02t0232400-01

Subcellular Localization

min:

: max

Winner_takes_all: plastid, nucleus

Predictor Summary:

plastid 2
extracellular 2
endoplasmic reticulum 2
vacuole 2
plasma membrane 2
golgi 2
mitochondrion 3
peroxisome 1
cytosol 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus, plastid
Any Predictor:	cytosol, mitochondrion, peroxisome, plastid, secretory
BaCelLo:	plastid
EpiLoc:	plastid
iPSORT:	secretory
MultiLoc:	mitochondrion
Plant-mPloc:	cytosol, peroxisome
PProwler:	mitochondrion
TargetP:	secretory
WoLF PSORT:	cytosol
YLoc:	mitochondrion

nucleus: 19621931
cytosol: 19685898
plastid: 21433289
golgi: 25047511
nucleus: 26035336
plasma membrane: 27800704
plastid: 27992503

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 19685898 doi

KA Doroshenk, AJ Crofts, RT Morris, JJ Wyrick, TW Okita

Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340, USA.

msms PMID: 26035336 doi

M Li, X Yin, K Sakata, P Yang, S Komatsu

§Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan., †Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China., ‡National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan.

msms PMID: 27992503 doi

S Xing, X Meng, L Zhou, H Mujahid, C Zhao, Y Zhang, C Wang, Z Peng

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, Mississippi, United States of America., Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice Research and Development Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing, Jiangsu, China.

msms PMID: 21433289 doi

S Reiland, J Grossmann, K Baerenfaller, P Gehrig, A Nunes-Nesi, AR Fernie, W Gruissem, S Baginsky

Department of Biology, Plant Biotechnology, ETH Zurich, Zurich, Switzerland.

msms PMID: 19621931 doi

T Aki, S Yanagisawa

Department of Applied Biological Chemistry, The University of Tokyo, Japan.

msms PMID: 25047511 doi

Y Nie, F Huang, S Dong, L Li, P Gao, H Zhao, Y Wang, S Han

Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, College of Life Sciences, Beijing, P. R. China.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os01t0611100-01
Os01t0687700-01
Os02t0232400-01
Os02t0269200-01
Os03t0702800-01
Os07t0587400-01
Os09t0544900-01
Os10t0577700-01
Os11t0132700-01
Os12t0129400-01
Os12t0129650-01
Os12t0131300-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G42790.1	Os03t0702800-01	AT1G12920.1	21798944
AT2G42790.1	Os07t0587400-01	AT1G12920.1	21798944
AT2G42790.1	Os09t0544900-01	AT1G76150.1	21798944
AT2G42790.1	Os10t0577700-01	AT2G35500.1	21798944
AT2G42790.1	Os02t0269200-01	AT3G16120.1	21798944
AT2G42790.1	Os11t0132700-01	AT3G56900.1	21798944
AT2G42790.1	Os12t0129400-01	AT3G56900.1	21798944
AT2G42790.1	Os12t0129650-01	AT3G56900.1	21798944
AT2G42790.1	Os12t0131300-01	AT3G56900.1	21798944
AT2G42790.1	Os02t0232400-01	AT3G58750.1	21798944
AT2G42790.1	Os01t0611100-01	AT5G55190.1	21798944
AT3G58740.1	Os02t0232400-01	AT2G42790.1	21798944
AT3G58750.1	Os01t0687700-01	AT1G25420.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
TraesCS5A01G413300.1	Wheat	mitochondrion	90.8	90.26
TraesCS5B01G416700.1	Wheat	golgi	90.6	90.06
EES04804	Sorghum	mitochondrion	90.6	89.7
TraesCS5D01G422000.1	Wheat	unclear	89.8	89.26
Zm00001d016248_P001	Maize	mitochondrion	89.4	88.87
HORVU5Hr1G099820.5	Barley	plastid	89.0	86.91
CDX79848	Canola	cytosol	38.4	86.88
CDY15651	Canola	nucleus	30.4	85.39
GSMUA_Achr10P...	Banana	mitochondrion	76.0	79.83
CDX98329	Canola	cytosol, peroxisome, plastid	80.8	79.68
VIT_12s0142g00610.t01	Wine grape	plastid	81.0	79.1
CDY50821	Canola	cytosol	80.6	79.02
AT2G42790.1	Thale cress	cytosol	80.2	78.78
CDX67764	Canola	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, peroxisome, plasma membrane, vacuole	80.0	78.74
Bra003355.1-P	Field mustard	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, peroxisome, plasma membrane, vacuole	79.8	78.54
Bra007417.1-P	Field mustard	cytosol	79.6	78.5
AT3G58750.1	Thale cress	endoplasmic reticulum, extracellular, golgi, mitochondrion, plasma membrane, vacuole	80.6	78.4
CDY49252	Canola	cytosol	79.4	78.3
CDX79851	Canola	cytosol	79.8	78.24
Bra004720.1-P	Field mustard	cytosol	79.8	78.24
PGSC0003DMT400020128	Potato	plastid	80.4	78.21
CDX71925	Canola	cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	79.8	78.08
Solyc12g011000.1.1	Tomato	nucleus	79.8	77.63
Solyc07g055840.2.1	Tomato	nucleus, unclear	79.0	77.3
PGSC0003DMT400044692	Potato	plastid	77.8	76.13
Bra007416.1-P	Field mustard	cytosol	67.8	75.5
CDY49249	Canola	cytosol	70.6	75.11
KRH14452	Soybean	nucleus	75.6	73.97
KRH73702	Soybean	cytosol, plastid	74.8	73.05
Bra014554.1-P	Field mustard	cytosol	68.0	72.34
CDY00587	Canola	cytosol	67.8	72.13
KRH46090	Soybean	endoplasmic reticulum, nucleus	73.6	71.73
KRG98876	Soybean	nucleus, plastid	73.4	71.4
AT3G58740.1	Thale cress	cytosol	68.2	71.04
CDX71926	Canola	cytosol	65.8	68.68
CDX89002	Canola	endoplasmic reticulum, golgi	35.2	62.19
HORVU0Hr1G030280.2	Barley	cytosol	22.2	61.67
CDY49250	Canola	extracellular	8.8	54.32
VIT_06s0080g00770.t01	Wine grape	cytosol	21.0	49.53
CDX71928	Canola	cytosol	69.2	41.84
Os11t0623601-00	Rice	extracellular	5.2	30.59
Os02t0194100-01	Rice	mitochondrion	21.2	22.46
Os11t0538900-01	Rice	mitochondrion	20.8	21.05

Protein Annotations

Gene3D:1.10.230.10	Gene3D:1.10.580.10	EntrezGene:4328812	MapMan:5.7.3.6.1	EMBL:AK120755	ProteinID:BAD27711.1
ProteinID:BAS77790.1	ProteinID:BAS77791.1	InterPro:Citrate_synth-like_lrg_a-sub	InterPro:Citrate_synth-like_sm_a-sub	InterPro:Citrate_synthase	InterPro:Citrate_synthase_AS
InterPro:Citrate_synthase_bac-typ	InterPro:Citrate_synthase_sf	GO:GO:0003674	GO:GO:0003824	GO:GO:0006091	GO:GO:0006099
GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0016740	GO:GO:0046912	InterPro:IPR016142
InterPro:IPR016143	EnsemblPlantsGene:Os02g0232400	EnsemblPlants:Os02t0232400-01	PFAM:PF00285	PIRSF:PIRSF001369	PRINTS:PR00143
ScanProsite:PS00480	PANTHER:PTHR11739	PANTHER:PTHR11739:SF4	UniProt:Q6EUF8	SUPFAM:SSF48256	UniParc:UPI00003CE4BC
RefSeq:XP_015626660.1	SEG:seg	:	:	:	:

Description

Similar to Citrate synthase. (Os02t0232400-01);Similar to Citrate synthase. (Os02t0232400-02)

Coordinates

chr2:+:7491229..7497708

Molecular Weight (calculated)

54643.6 Da

IEP (calculated)

9.152

GRAVY (calculated)

-0.170

Length

500 amino acids

Sequence
(BLAST)

001: MDRARLAVLS AHLASPAAAC GEADAAGPLE RSAASAGARG GALAVVDGRT GKRYEVKVSD EGTVRATDFK KITTGKDDKG LKIYDPGYLN TAPVRSSICY
101: IDGDEGILRY RGYPIEELAE SSSFVEVAYL LMYGSLPTQS QLAGWEFAIS QHSAVPQGLL DIIQAMPHDA HPMGALASAM STLSVFHPDA NPALRGQDLY
201: KSKQVRDKQI VRVLGKAPTI AAAAYLRLAG RPPILPTNSL SYSENFLYML DSLGDKEYKP NPRLARVLDI LFILHAEHEM NCSTAAARHL ASSGVDVFTA
301: LSGAVGALYG PLHGGANEAV LKMLNEIGSV ENIPDFIEGV KNRKRKMSGF GHRVYKNYDP RAKVIRKLAE EVFSIVGRDP LIEVAVALEK AALSDDYFVK
401: RKLYPNVDFY SGLIYRAMGF PTEFFPVLFA IPRMAGWLAH WKESLDDPDT KIMRPQQVYT GVWLRHYTPV RERVPASQGE QLGQIATSNA TRRRRAGSAL

Best Arabidopsis Sequence Match ( AT2G42790.1 )
(BLAST)

001: MEISERVRAR LAVLSGHLSE GKQDSPAIER WCTSADTSVA PLGSLKGTLT IVDERTGKNY KVPVSDDGTV KAVDFKKIVT GKEDKGLKLY DPGYLNTAPV
101: RSSISYIDGD EGILRYRGYP IEEMAENSTF LEVAYLLMYG NLPSESQLSD WEFAVSQHSA VPQGVLDIIQ SMPHDAHPMG VLVSAMSALS IFHPDANPAL
201: RGQDIYDSKQ VRDKQIIRII GKAPTIAAAA YLRMAGRPPV LPSGNLPYAD NFLYMLDSLG NRSYKPNPRL ARVLDILFIL HAEHEMNCST AAARHLASSG
301: VDVYTAVAGA VGALYGPLHG GANEAVLKML SEIGTVENIP EFIEGVKNRK RKMSGFGHRV YKNYDPRAKV IKNLADEVFS IVGKDPLIEV AVALEKAALS
401: DDYFVKRKLY PNVDFYSGLI YRAMGFPPEF FTVLFAIPRM AGYLSHWKES LDDPDTKIMR PQQVYTGVWL RHYTPVRERI VTDDSKESDK LGQVATSNAS
501: RRRLAGSSV

Arabidopsis Description

CSY3Citrate synthase [Source:UniProtKB/TrEMBL;Acc:Q0WUX6]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.