CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

nucleus 2
mitochondrion 3
cytosol 2
plasma membrane 1
plastid 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG93747
KRH01441
KRH18315
KRH56393
KRH62532

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G19320.1	KRH56393	AT4G26455.1	17600715
AT5G19320.1	KRH62532	AT4G26455.1	17600715
AT5G19320.1	KRG93747	AT5G11390.1	18591351
AT5G19320.1	KRH01441	AT5G11390.1	18591351
AT5G19320.1	KRH18315	AT5G11390.1	18591351
AT5G19320.1	KRH56393	AT5G56210.1	17600715
AT5G19320.1	KRH62532	AT5G56210.1	17600715

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH35557	Soybean	endoplasmic reticulum, nucleus	92.29	92.12
VIT_05s0020g02970.t01	Wine grape	cytosol, plastid	73.87	72.91
VIT_19s0014g03800.t01	Wine grape	cytosol, plastid	69.17	69.96
KRH32020	Soybean	cytosol	69.74	69.48
KRH71426	Soybean	nucleus	69.36	69.23
AT5G19320.1	Thale cress	cytosol	70.49	68.81
Solyc01g079680.2.1	Tomato	plastid	70.11	67.82
PGSC0003DMT400023310	Potato	plastid	69.36	66.73
CDX92540	Canola	cytosol	68.8	65.36
CDY02566	Canola	cytosol	24.06	64.65
CDY16082	Canola	cytosol	13.35	62.83
Os05t0543200-01	Rice	plasma membrane	63.91	62.5
GSMUA_Achr11P...	Banana	cytosol, plastid	64.1	62.23
EES05430	Sorghum	cytosol, nucleus, plastid	63.35	62.06
Zm00001d051112_P001	Maize	plasma membrane	59.96	61.82
EES05435	Sorghum	cytosol, plastid	64.66	61.65
Zm00001d017239_P001	Maize	mitochondrion, plasma membrane	60.53	61.57
TraesCS1B01G266900.1	Wheat	plastid	63.72	61.52
TraesCS1A01G256200.1	Wheat	cytosol	63.35	61.27
TraesCS1D01G255600.1	Wheat	cytosol, plastid	63.35	60.72
Bra026911.1-P	Field mustard	cytosol	15.04	60.61
EES07543	Sorghum	cytosol	28.38	60.16
TraesCS3D01G394300.1	Wheat	cytosol, plastid	62.59	60.11
HORVU3Hr1G090080.1	Barley	plastid	62.03	59.57
TraesCS3B01G433100.1	Wheat	cytosol, plastid	62.03	59.57
TraesCS3A01G400100.1	Wheat	plastid	62.41	56.37
HORVU1Hr1G063300.3	Barley	plastid	64.29	52.37
Zm00001d034691_P001	Maize	cytosol	34.77	48.68
Zm00001d052426_P001	Maize	cytosol	12.03	46.72
GSMUA_Achr8P13660_001	Banana	cytosol	50.94	44.28
AT3G06000.1	Thale cress	cytosol	16.92	42.65
Os05t0409200-00	Rice	cytosol	8.46	42.06
CDY27720	Canola	cytosol, mitochondrion	16.54	36.21
Bra020788.1-P	Field mustard	cytosol, mitochondrion	15.98	36.02
CDY05221	Canola	cytosol, mitochondrion, nucleus, plastid	12.41	34.55
Zm00001d043604_P002	Maize	cytosol	33.08	32.77
Zm00001d046105_P001	Maize	peroxisome	33.08	29.93
KRH69472	Soybean	nucleus	18.98	16.67
KRH74682	Soybean	plastid	18.42	16.17

Protein Annotations

Gene3D:1.10.246.200	EntrezGene:100795339	MapMan:23.5.1.5	Gene3D:3.80.10.10	EMBL:ACUP02012673	EnsemblPlantsGene:GLYMA_20G143300
GO:GO:0003674	GO:GO:0005488	GO:GO:0005515	UniProt:I1NGB7	InterPro:IPR001611	InterPro:IPR032675
InterPro:IPR038214	ProteinID:KRG91255.1	EnsemblPlants:KRG91256	ProteinID:KRG91256	ProteinID:KRG91256.1	InterPro:LRR_dom_sf
InterPro:Leu-rich_rpt	PFAM:PF13516	PFAM:PF13943	PANTHER:PTHR24106	PANTHER:PTHR24106:SF248	SMART:SM00368
SUPFAM:SSF52047	UniParc:UPI000233DD5D	InterPro:WPP_dom	InterPro:WPP_sf	SEG:seg	:

Description

hypothetical protein

Coordinates

chr20:-:38188187..38191151

Molecular Weight (calculated)

57927.5 Da

IEP (calculated)

4.341

GRAVY (calculated)

-0.295

Length

532 amino acids

Sequence
(BLAST)

001: MELNSQKRPF SIKLWPPSQN TRQTLVERMT NNLTTKSLFT QKYGTLDKEE AEENAKRIED VAFATANLHY EKEPDGDGGS AVQLYAKECS KLLLDVLKRG
101: PSKKDDEVVT SVNTTSSLEY VFDISKGQRA FIEADETEQL LSPLKEPGNS YTKICFSNRS FGLGAAQIAE PILTSIKDQL KEVDLSDFIA GRSEAEALDV
201: MKIFSTALEG SVLRSLNLSD NALGEKGVRA FGALLKSQKC LEELYLMNDG ISKEAALAVC ELIPFTEKLK VLHFHNNMTG DEGALAIAEV VKRSPLLEDF
301: RCSSTRIGSE GGVALSDALG NCAHLKKLDL RDNMLGVEGG VSLSKALTKQ AELREVYLSY LNLEDDGAIA IVDALTEAAP HLEVLEMSGN DITADAAPAI
401: AACLAAKQFL TKLNLSENEL KDEGANLITK AIEGHVQLKE IDLSANQISR AGAQQLAVTV VQKADFKLLN INGNFISDEG IDELKDIFKK SPDMLGPLDE
501: NDPDGIDNDE ESDEEGGADE LESKMKNLVV DD

Best Arabidopsis Sequence Match ( AT5G19320.2 )
(BLAST)

001: MADILDSRPH AFSIKLWPPS LPTRKALIER ITNNFSSKTI FTEKYGSLTK DQATENAKRI EDIAFSTANQ QFEREPDGDG GSAVQLYAKE CSKLILEVLK
101: KGPVAKVAAR ELISEDSVSP RETFFDISKG KRAFIEAEEA EELLKPLKEP GNAYTKICFS NRSFGLGAAR VAEPILASLK DQLKEVDLSD FVAGRPELEA
201: LEVMNIFSDA LQGSILSSLN LSDNALGEKG VRAFGALLKS LSSLEELYLM NDGISKEAAQ AVSELIPSTE NLRVLHFHNN MTGDEGALAI AEVVKRSPLL
301: ENFRCSSTRV GSKGGIALSE ALEHCTHMEK LDLRDNMFGT EAGVSLSKTL SSFKHMTELY LSYLNLEDEG AIAIVNALKE SASPIEVLEM AGNDITVEAA
401: SAIAACVAAK QDLNKLNLSE NELKDEGCVQ IANCIEEGHS KLQYIDMSTN YIRRAGARAL AHVVVKKEAF KLLNIDGNII SEEGIEELKE IFKKSPELLG
501: ALDENDPDGE EDDDDEEDEE DEENEGNGNG ELESKLKNLE VNQED

Arabidopsis Description

RANGAP2RANGAP2 [Source:UniProtKB/TrEMBL;Acc:A0A178UQD1]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.