CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, nucleus

Predictor Summary:

nucleus 2
plastid 4
cytosol 2
plasma membrane 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG93747
KRH01441
KRH18315
KRH56393
KRH62532

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G19320.1	KRH56393	AT4G26455.1	17600715
AT5G19320.1	KRH62532	AT4G26455.1	17600715
AT5G19320.1	KRG93747	AT5G11390.1	18591351
AT5G19320.1	KRH01441	AT5G11390.1	18591351
AT5G19320.1	KRH18315	AT5G11390.1	18591351
AT5G19320.1	KRH56393	AT5G56210.1	17600715
AT5G19320.1	KRH62532	AT5G56210.1	17600715

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRG91256	Soybean	endoplasmic reticulum	92.12	92.29
VIT_05s0020g02970.t01	Wine grape	cytosol, plastid	72.42	71.61
VIT_19s0014g03800.t01	Wine grape	cytosol, plastid	68.86	69.77
AT5G19320.1	Thale cress	cytosol	70.36	68.81
KRH32020	Soybean	cytosol	68.11	67.98
KRH71426	Soybean	nucleus	67.54	67.54
Solyc01g079680.2.1	Tomato	plastid	68.86	66.73
PGSC0003DMT400023310	Potato	plastid	68.29	65.82
CDX92540	Canola	cytosol	68.48	65.18
CDY02566	Canola	cytosol	24.2	65.15
EES05430	Sorghum	cytosol, nucleus, plastid	64.17	62.98
Os05t0543200-01	Rice	plasma membrane	63.6	62.32
GSMUA_Achr11P...	Banana	cytosol, plastid	63.79	62.04
CDY16082	Canola	cytosol	13.13	61.95
EES05435	Sorghum	cytosol, plastid	64.54	61.65
Zm00001d051112_P001	Maize	plasma membrane	59.29	61.24
Zm00001d017239_P001	Maize	mitochondrion, plasma membrane	59.85	60.99
TraesCS1B01G266900.1	Wheat	plastid	63.04	60.98
TraesCS1A01G256200.1	Wheat	cytosol	62.66	60.73
TraesCS1D01G255600.1	Wheat	cytosol, plastid	62.66	60.18
EES07543	Sorghum	cytosol	28.14	59.76
TraesCS3D01G394300.1	Wheat	cytosol, plastid	62.1	59.75
HORVU3Hr1G090080.1	Barley	plastid	61.54	59.21
TraesCS3B01G433100.1	Wheat	cytosol, plastid	61.54	59.21
Bra026911.1-P	Field mustard	cytosol	14.63	59.09
TraesCS3A01G400100.1	Wheat	plastid	61.91	56.03
HORVU1Hr1G063300.3	Barley	plastid	63.6	51.91
Zm00001d034691_P001	Maize	cytosol	34.71	48.68
Zm00001d052426_P001	Maize	cytosol	11.63	45.26
GSMUA_Achr8P13660_001	Banana	cytosol	50.84	44.28
AT3G06000.1	Thale cress	cytosol	17.07	43.13
Os05t0409200-00	Rice	cytosol	8.63	42.99
Bra020788.1-P	Field mustard	cytosol, mitochondrion	15.95	36.02
CDY27720	Canola	cytosol, mitochondrion	16.14	35.39
CDY05221	Canola	cytosol, mitochondrion, nucleus, plastid	12.38	34.55
Zm00001d043604_P002	Maize	cytosol	33.02	32.77
Zm00001d046105_P001	Maize	peroxisome	33.02	29.93
KRH69472	Soybean	nucleus	19.51	17.16
KRH74682	Soybean	plastid	18.76	16.5

Protein Annotations

Gene3D:1.10.246.200	EntrezGene:100785942	MapMan:23.5.1.5	Gene3D:3.80.10.10	EMBL:ACUP02006690	EnsemblPlantsGene:GLYMA_10G250400
GO:GO:0003674	GO:GO:0005488	GO:GO:0005515	InterPro:IPR001611	InterPro:IPR032675	InterPro:IPR038214
UniProt:K7LLB1	ProteinID:KRH35556.1	EnsemblPlants:KRH35557	ProteinID:KRH35557	ProteinID:KRH35557.1	ProteinID:KRH35558.1
InterPro:LRR_dom_sf	InterPro:Leu-rich_rpt	PFAM:PF13516	PFAM:PF13943	PANTHER:PTHR24106	PANTHER:PTHR24106:SF248
SMART:SM00368	SUPFAM:SSF52047	UniParc:UPI000233CAEA	InterPro:WPP_dom	InterPro:WPP_sf	SEG:seg

Description

hypothetical protein

Coordinates

chr10:+:47845125..47848173

Molecular Weight (calculated)

58045.6 Da

IEP (calculated)

4.415

GRAVY (calculated)

-0.336

Length

533 amino acids

Sequence
(BLAST)

001: MELNSQPRPF SIKLWPPSQN TRQTLVERMT NNLTTKSIFT QKYGTLDQEK AEENAKRIED VAFATANLHY EKEPDGDGGS AVQLYAKECS KLLLDVLKRG
101: PSKKDDEEVV ASVNTTAPHE SVFDISKGQR AFIEADEAQQ LLSPLKEPGN SFTKICFSNR SFGLGAAQVA EPILTAIKDQ LKEVDLSDFI AGRSEVEALD
201: VMKIFSTALE GSVLRSLNLS DNALGEKGVR AFGALLKSQK CLEELYLMND GISKEAARAV CELIPFTEKL KVLHFHNNMT GDEGALAIAE VVKRSPLLED
301: FRCSSTRIGA EGGVALSDAL GSCAHLKKLD LRDNMLGVDG GVSLSKALSK HAELREVYLS YLNLEDDGAI AIVDALKESA PHLEVLEMSG NDSTADAAPA
401: IAACLEAKQF LSKLNLSENE LKDEGAKLIT KAIEGHVQLK EIDLSTNQIR KDGAQQLAVT VVQKADFKLL NINGNFISDE GIDELKDIFK KSPDMLGPLD
501: ENDPDGVDDD EESDEEGGAD ELESKMKNLV VDD

Best Arabidopsis Sequence Match ( AT5G19320.2 )
(BLAST)

001: MADILDSRPH AFSIKLWPPS LPTRKALIER ITNNFSSKTI FTEKYGSLTK DQATENAKRI EDIAFSTANQ QFEREPDGDG GSAVQLYAKE CSKLILEVLK
101: KGPVAKVAAR ELISEDSVSP RETFFDISKG KRAFIEAEEA EELLKPLKEP GNAYTKICFS NRSFGLGAAR VAEPILASLK DQLKEVDLSD FVAGRPELEA
201: LEVMNIFSDA LQGSILSSLN LSDNALGEKG VRAFGALLKS LSSLEELYLM NDGISKEAAQ AVSELIPSTE NLRVLHFHNN MTGDEGALAI AEVVKRSPLL
301: ENFRCSSTRV GSKGGIALSE ALEHCTHMEK LDLRDNMFGT EAGVSLSKTL SSFKHMTELY LSYLNLEDEG AIAIVNALKE SASPIEVLEM AGNDITVEAA
401: SAIAACVAAK QDLNKLNLSE NELKDEGCVQ IANCIEEGHS KLQYIDMSTN YIRRAGARAL AHVVVKKEAF KLLNIDGNII SEEGIEELKE IFKKSPELLG
501: ALDENDPDGE EDDDDEEDEE DEENEGNGNG ELESKLKNLE VNQED

Arabidopsis Description

RANGAP2RANGAP2 [Source:UniProtKB/TrEMBL;Acc:A0A178UQD1]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.