CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, nucleus

Predictor Summary:

nucleus 1
vacuole 1
cytosol 3
mitochondrion 1
plastid 1

Predictors

GFP

MS/MS

Papers

endoplasmic reticulum: 21107622
nucleus: 21132161
endoplasmic reticulum: 23041469
nucleus: 24385303
endoplasmic reticulum: 27224218
mitochondrion: 28499913
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 21107622 doi

N Ahsan, T Nakamura, S Komatsu

National Institute of Crop Science, 2-1-18 Kannondai, Tsukuba, 305-8518, Japan.

msms PMID: 23041469 doi

S Komatsu, R Kuji, Y Nanjo, S Hiraga, K Furukawa

National Institute of Crop Science, Tsukuba 305-8518, Japan. skomatsu@affrc.go.jp

msms PMID: 24385303 doi

S Komatsu, S Hiraga, MZ Nouri

National Institute of Crop Science, National Agriculture and Food Research Organization, Kannondai 2-1-18, Tsukuba, 305-8518, Japan, skomatsu@affrc.go.jp.

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG95733
KRH07815
KRH28950
KRH39397
KRH67452
KRH76512

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G48630.1	KRH07815	AT4G35470.1	21798944
AT1G48630.1	KRH28950	AT4G35470.1	21798944
AT1G48630.1	KRH39397	AT4G35470.1	21798944
AT1G48630.1	KRH76512	AT4G35470.1	21798944
AT1G48630.1	KRG95733	AT5G43960.1	21798944
AT1G48630.1	KRH67452	AT5G43960.1	21798944
AT3G18130.1	KRH07815	AT4G35470.1	21798944
AT3G18130.1	KRH28950	AT4G35470.1	21798944
AT3G18130.1	KRH39397	AT4G35470.1	21798944
AT3G18130.1	KRH76512	AT4G35470.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH41792	Soybean	nucleus	98.15	98.15
VIT_17s0000g02750.t01	Wine grape	cytosol, plastid	92.31	91.74
CDX83869	Canola	cytosol	67.69	88.0
CDY26186	Canola	cytosol	67.38	87.6
CDY60778	Canola	cytosol, plastid	67.08	87.2
CDX96736	Canola	cytosol	67.08	87.2
CDX81860	Canola	cytosol	66.77	86.8
Bra025931.1-P	Field mustard	cytosol	87.08	86.28
Bra016570.1-P	Field mustard	cytosol	86.77	86.24
AT1G48630.1	Thale cress	cytosol	86.46	86.2
AT3G18130.1	Thale cress	cytosol	86.46	86.2
Solyc03g119040.2.1	Tomato	nucleus	86.46	86.2
CDY21720	Canola	cytosol	66.15	86.0
AT1G18080.1	Thale cress	cytosol	86.46	85.93
Bra031014.1-P	Field mustard	cytosol	86.46	85.93
PGSC0003DMT400014419	Potato	cytosol	85.54	85.02
CDX92200	Canola	extracellular	84.92	84.92
CDX95784	Canola	cytosol	84.92	84.92
CDY71917	Canola	cytosol	63.69	84.84
CDY18497	Canola	cytosol	84.92	84.66
Bra022282.1-P	Field mustard	cytosol	84.62	84.62
Solyc06g069010.2.1	Tomato	nucleus	84.62	84.36
PGSC0003DMT400074094	Potato	cytosol	84.62	84.36
CDX95554	Canola	cytosol	84.62	84.36
PGSC0003DMT400040923	Potato	cytosol	84.62	83.59
Solyc12g040510.1.1	Tomato	cytosol	84.62	83.59
CDY59680	Canola	cytosol	57.85	83.56
Bra021294.1-P	Field mustard	cytosol	83.38	83.13
Bra032261.1-P	Field mustard	cytosol	83.38	82.87
CDY64888	Canola	cytosol	75.08	81.61
Bra018745.1-P	Field mustard	cytosol	74.46	81.21
PGSC0003DMT400041253	Potato	cytosol	21.23	81.18
CDX86074	Canola	cytosol	73.54	80.2
CDX93686	Canola	cytosol	73.54	80.2
CDY44104	Canola	cytosol	73.54	80.2
Os01t0686800-02	Rice	cytosol	75.08	73.05
CDY52180	Canola	cytosol	36.31	72.84
Zm00001d038923_P001	Maize	plastid	74.77	72.75
Zm00001d009480_P001	Maize	plasma membrane, plastid	74.46	72.46
EES18632	Sorghum	cytosol	74.15	72.16
TraesCS3D01G264000.1	Wheat	cytosol	72.62	71.08
TraesCS3B01G297300.1	Wheat	nucleus	72.62	71.08
HORVU3Hr1G063670.2	Barley	cytosol	72.62	71.08
TraesCS1B01G398800.1	Wheat	plastid	73.23	71.04
EES01286	Sorghum	cytosol	72.31	70.78
TraesCS3A01G263900.1	Wheat	nucleus	72.31	70.78
TraesCS1A01G378000.1	Wheat	cytosol, golgi, nucleus, unclear	72.92	70.75
TraesCS1D01G385000.1	Wheat	cytosol	72.31	70.15
Os05t0552300-02	Rice	cytosol	71.38	69.05
HORVU1Hr1G083710.1	Barley	cytosol	68.31	63.25
GSMUA_Achr2P14580_001	Banana	cytosol	55.08	57.37
KRH48610	Soybean	cytosol	58.77	56.18
Bra000607.1-P	Field mustard	cytosol	56.31	46.21
CDX77303	Canola	cytosol	30.15	31.72
CDX77299	Canola	plastid	6.77	22.68
CDY46056	Canola	cytosol, endoplasmic reticulum, plasma membrane	43.08	15.57
KRH11768	Soybean	cytosol, mitochondrion, nucleus	11.08	14.01
KRH36772	Soybean	nucleus	19.08	12.5

Protein Annotations

MapMan:17.1.3.1.33	Gene3D:2.130.10.10	EMBL:ACUP02003367	InterPro:G-protein_beta_WD-40_rep	EnsemblPlantsGene:GLYMA_05G243800	GO:GO:0003674
GO:GO:0005488	GO:GO:0005515	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737
GO:GO:0005840	GO:GO:0030529	UniProt:I1K5E6	InterPro:IPR001680	InterPro:IPR015943	InterPro:IPR017986
EnsemblPlants:KRH60497	ProteinID:KRH60497	ProteinID:KRH60497.1	PFAM:PF00400	PRINTS:PR00320	ScanProsite:PS00678
PFscan:PS50082	PFscan:PS50294	PANTHER:PTHR19868	PANTHER:PTHR19868:SF3	SMART:SM00320	SUPFAM:SSF50978
UniParc:UPI00023C4976	InterPro:WD40/YVTN_repeat-like_dom_sf	InterPro:WD40_repeat	InterPro:WD40_repeat_CS	InterPro:WD40_repeat_dom	InterPro:WD40_repeat_dom_sf

Description

hypothetical protein

Coordinates

chr5:+:41837252..41839280

Molecular Weight (calculated)

35665.2 Da

IEP (calculated)

7.808

GRAVY (calculated)

-0.154

Length

325 amino acids

Sequence
(BLAST)

001: MAEGLVLRGT MRAHTDVVTA IATPIDNSDM IVTASRDKSI ILWHLTKEDK TYGVPRRRLT GHSHFVQDVV LSSDGQFALS GSWDGELRLW DLAAGTSARR
101: FVGHTKDVLS VAFSIDNRQI VSASRDRTIK LWNTLGECKY TIQDGDAHSD WVSCVRFSPS TLQPTIVSAS WDRTVKVWNL TNCKLRNTLA GHNGYVNTVA
201: VSPDGSLCAS GGKDGVILLW DLAEGKRLYS LDAGSIIHAL CFSPNRYWLC AATEQSIKIW DLESKSIVED LKVDLKTEAD ATSGGGNANK KKVIYCTSLN
301: WSADGSTLFS GYTDGVVRVW AIGRY

Best Arabidopsis Sequence Match ( AT3G18130.1 )
(BLAST)

001: MAEGLVLKGI MRAHTDIVTA IATPIDNSDI IVTASRDKSI ILWKLTKDDK SYGVAQRRLT GHSHFVEDVV LSSDGQFALS GSWDGELRLW DLATGETTRR
101: FVGHTKDVLS VAFSTDNRQI VSASRDRTIK LWNTLGECKY TISEGDGHKE WVSCVRFSPN TLVPTIVSAS WDKTVKVWNL QNCKLRNSLV GHSGYLNTVA
201: VSPDGSLCAS GGKDGVILLW DLAEGKKLYS LEAGSIIHSL CFSPNRYWLC AATENSIRIW DLESKSVVED LKVDLKSEAE KNEGGVGTGN QKKVIYCTSL
301: NWSADGSTLF SGYTDGVVRV WGIGRY

Arabidopsis Description

RACK1CReceptor for activated C kinase 1C [Source:UniProtKB/Swiss-Prot;Acc:Q9LV28]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.