CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: mitochondrion, nucleus

Predictor Summary:

plastid 4
cytosol 3
mitochondrion 2

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
mitochondrion: 27780359
mitochondrion: 28499913
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27780359 doi

G Mustafa, S Komatsu

Graduate School of Life and Environmental Science, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH44804
KRH56323
KRH62783

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G28510.1	KRH44804	AT5G40770.1	12837548
AT4G28510.1	KRH56323	AT5G40770.1	12837548
AT4G28510.1	KRH62783	AT5G40770.1	12837548

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH39037	Soybean	mitochondrion	80.06	93.08
KRH22328	Soybean	cytosol, mitochondrion	70.24	89.73
KRH29315	Soybean	endoplasmic reticulum, mitochondrion	76.79	89.27
KRH24002	Soybean	mitochondrion	75.6	87.89
PGSC0003DMT400056251	Potato	cytosol, golgi, mitochondrion	75.6	87.89
Bra011035.1-P	Field mustard	cytosol, mitochondrion, plastid	74.7	86.55
CDY38382	Canola	cytosol, mitochondrion, plastid	74.7	86.55
CDY35195	Canola	cytosol, mitochondrion, plastid	74.7	86.55
Solyc10g008140.2.1	Tomato	plastid	74.4	86.51
AT4G28510.1	Thale cress	cytosol, mitochondrion, plastid	73.21	85.42
CDY14238	Canola	cytosol	61.01	84.71
Bra010364.1-P	Field mustard	cytosol	60.71	84.3
PGSC0003DMT400035734	Potato	cytosol, mitochondrion, plastid	72.32	83.22
CDY10095	Canola	golgi	70.24	81.94
CDY15478	Canola	endoplasmic reticulum	69.94	81.6
CDX89860	Canola	endoplasmic reticulum	69.94	81.6
Bra030546.1-P	Field mustard	endoplasmic reticulum	69.94	81.6
AT1G03860.3	Thale cress	endoplasmic reticulum	69.35	81.47
CDY32517	Canola	golgi	69.64	81.25
Bra032539.1-P	Field mustard	golgi	69.64	81.25
TraesCS5B01G528100.1	Wheat	endoplasmic reticulum, plasma membrane	68.15	81.21
AT2G20530.2	Thale cress	cytosol, mitochondrion, plastid	68.75	80.77
Zm00001d034795_P002	Maize	mitochondrion	69.05	80.28
Zm00001d005485_P001	Maize	mitochondrion	69.05	80.28
EER98334	Sorghum	mitochondrion	69.05	80.28
Solyc12g005500.1.1	Tomato	plastid	67.56	80.21
CDY18191	Canola	endoplasmic reticulum	68.75	80.21
GSMUA_Achr8P08390_001	Banana	mitochondrion	69.64	80.14
Os07t0262200-02	Rice	mitochondrion, plasma membrane, plastid	68.75	79.93
GSMUA_Achr11P...	Banana	unclear	69.35	79.79
GSMUA_Achr7P03130_001	Banana	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, plasma membrane, plastid, vacuole	68.75	79.66
CDX97444	Canola	cytosol, mitochondrion, plastid	61.31	79.23
Bra015273.1-P	Field mustard	endoplasmic reticulum	67.86	79.17
EER90545	Sorghum	cytosol, mitochondrion, peroxisome	67.86	78.89
TraesCS1A01G407100.1	Wheat	plastid	67.56	78.82
TraesCS5D01G527100.1	Wheat	golgi, unclear	67.56	78.55
CDX86666	Canola	mitochondrion	70.54	77.45
AT5G44140.1	Thale cress	golgi	63.99	77.34
Os03t0841700-02	Rice	mitochondrion	65.48	75.86
VIT_19s0085g00730.t01	Wine grape	golgi, vacuole	75.6	74.71
VIT_15s0048g02350.t01	Wine grape	cytosol	75.3	69.7
HORVU5Hr1G119470.2	Barley	mitochondrion	44.05	66.37
TraesCS4A01G344900.1	Wheat	mitochondrion, plastid	50.89	63.81
Zm00001d019341_P001	Maize	mitochondrion	17.56	63.44
Os03t0813200-00	Rice	cytosol, mitochondrion, plasma membrane	22.02	62.71
VIT_16s0100g00070.t01	Wine grape	cytosol, extracellular, golgi	13.1	61.97
HORVU5Hr1G119700.3	Barley	mitochondrion, plastid	52.98	61.17
GSMUA_Achr6P12800_001	Banana	cytosol	16.96	49.14
KRH62783	Soybean	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, plasma membrane, plastid, vacuole	36.61	44.09
KRH44804	Soybean	mitochondrion	36.61	43.93
KRH56323	Soybean	mitochondrion	36.31	43.73
Os03t0814600-00	Rice	cytosol	53.27	41.53
GSMUA_Achr6P12790_001	Banana	golgi	23.81	33.33

Protein Annotations

EMBL:ACUP02007760	EnsemblPlants:KRH26997	EnsemblPlantsGene:GLYMA_12G207500	EntrezGene:100793227	Gene3D:3.30.479.30	GO:GO:0005575
GO:GO:0016020	InterPro:Band_7	InterPro:Band_7/SPFH_dom_sf	InterPro:Prohibitin	ncoils:Coil	PANTHER:PTHR23222
PANTHER:PTHR23222:SF4	PFAM:PF01145	PRINTS:PR00679	ProteinID:KRH26997	ProteinID:KRH26997.1	SEG:seg
SMART:SM00244	SUPFAM:SSF117892	UniParc:UPI00023D0FE6	UniProt:I1LUJ6	MapMan:35.1	:

Description

hypothetical protein

Coordinates

chr12:-:36730431..36734124

Molecular Weight (calculated)

37148.9 Da

IEP (calculated)

10.178

GRAVY (calculated)

-0.228

Length

336 amino acids

Sequence
(BLAST)

001: MHLLSFSFSC WESLNPTEKR YINPATNNQP PNPSLFSASI GNFQGKKMNL KNMKIPNVPG GGASSALLKL GLLGGIGLYA AANSLYNVEG GHRAIVFNRL
101: VGVKDKVYPE GTHFMIPWFE RPVIYDVRAR PHLVESTSGS RDLQMVKIGL RVLTRPLPNQ LPTVYRTLGE NYNERVLPSI IHETLKAVVA QYNASQLITQ
201: REAVSREIRK ILTERAANFN IALDDVSITS LTFGKEFTAA IEAKQVAAQE AERAKFVVEK AEQDKRSAVI RAQGEAKSAQ LIGQAIANNP AFITLRKIEA
301: AREIAHTISN AANKVYLNSD DLLLNLQEMK LEPGRK

Best Arabidopsis Sequence Match ( AT1G03860.1 )
(BLAST)

001: MSFNKVPNIP GAPALSALLK VSVIGGLGVY ALTNSLYNVD GGHRAVMFNR LTGIKEKVYP EGTHFMVPWF ERPIIYDVRA RPYLVESTTG SHDLQMVKIG
101: LRVLTRPMGD RLPQIYRTLG ENYSERVLPS IIHETLKAVV AQYNASQLIT QREAVSREIR KILTERASNF DIALDDVSIT TLTFGKEFTA AIEAKQVAAQ
201: EAERAKFIVE KAEQDRRSAV IRAQGEAKSA QLIGQAIANN QAFITLRKIE AAREIAQTIA QSANKVYLSS NDLLLNLQEM NLEPKK

Arabidopsis Description

PHB2PHB2 [Source:UniProtKB/TrEMBL;Acc:A0A178WBG3]
SUBAcon: [endoplasmic reticulum]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.