Factsheet for HORVU2Hr1G008310.1

Subcellular Localization

min:

: max

Winner_takes_all: golgi, vacuole

Predictor Summary:

extracellular 2
endoplasmic reticulum 2
vacuole 3
plasma membrane 2
golgi 3

Predictors

GFP

MS/MS

Papers

Winner Takes All:	golgi, vacuole
Any Predictor:	secretory
iPSORT:	secretory
MultiLoc:	vacuole
TargetP:	secretory
YLoc:	golgi

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
TraesCS2A01G052900.1	Wheat	unclear	87.66	90.15
TraesCS2D01G052400.1	Wheat	vacuole	62.06	83.83
HORVU2Hr1G008270.1	Barley	peroxisome	45.12	78.78
HORVU2Hr1G008330.1	Barley	extracellular, plasma membrane, vacuole	75.69	77.55
HORVU2Hr1G008300.1	Barley	plastid	73.11	75.48
HORVU7Hr1G089160.3	Barley	vacuole	63.54	65.71
Os06t0549600-01	Rice	extracellular	58.38	60.27
HORVU4Hr1G086520.1	Barley	plasma membrane, vacuole	52.3	54.93
HORVU2Hr1G122590.1	Barley	vacuole	53.59	54.39
HORVU4Hr1G017270.1	Barley	vacuole	53.04	53.04
HORVU7Hr1G093940.1	Barley	vacuole	51.01	52.26
HORVU3Hr1G020240.1	Barley	extracellular, plasma membrane, vacuole	50.46	51.7
HORVU2Hr1G021650.2	Barley	golgi, mitochondrion, plastid, vacuole	53.78	50.43
HORVU7Hr1G089190.1	Barley	vacuole	48.25	49.9
HORVU7Hr1G089280.1	Barley	mitochondrion, peroxisome, plasma membrane	48.62	49.07
HORVU1Hr1G092680.1	Barley	golgi, vacuole	48.43	49.07
HORVU7Hr1G089270.1	Barley	vacuole	48.07	49.06
HORVU2Hr1G000540.1	Barley	golgi, vacuole	47.51	47.51
PGSC0003DMT400080465	Potato	cytosol	34.44	46.06
GSMUA_Achr2P15490_001	Banana	golgi, vacuole	35.36	45.82
HORVU2Hr1G000530.1	Barley	plasma membrane, vacuole	36.83	45.66
HORVU2Hr1G013930.1	Barley	golgi, vacuole	45.86	45.6
KRH36852	Soybean	plastid	37.2	45.6
GSMUA_Achr4P31040_001	Banana	vacuole	43.83	44.57
HORVU7Hr1G089210.1	Barley	mitochondrion	49.91	44.14
KRH42274	Soybean	mitochondrion, nucleus	42.17	43.79
PGSC0003DMT400080468	Potato	vacuole	42.54	43.75
CDY59834	Canola	peroxisome, plasma membrane, plastid	36.1	43.65
PGSC0003DMT400054391	Potato	cytosol, nucleus, peroxisome	34.62	43.62
KRH42808	Soybean	extracellular, vacuole	42.73	43.36
Solyc06g065060.1.1	Tomato	extracellular	42.91	43.31
CDY66956	Canola	peroxisome, plasma membrane, plastid	33.52	43.23
KRH42266	Soybean	vacuole	42.91	43.23
Bra024719.1-P	Field mustard	peroxisome, plastid, vacuole	35.91	43.14
VIT_03s0017g01470.t01	Wine grape	vacuole	24.86	43.13
Solyc06g065070.1.1	Tomato	vacuole	42.36	42.91
VIT_12s0028g01780.t01	Wine grape	plasma membrane, vacuole	42.36	42.91
KRH58387	Soybean	plastid, vacuole	43.28	42.81
KRH42273	Soybean	nucleus	41.44	42.69
PGSC0003DMT400080467	Potato	vacuole	42.17	42.57
KRH11844	Soybean	vacuole	42.36	42.51
CDY69093	Canola	vacuole	41.8	42.43
CDY31432	Canola	vacuole	41.44	42.37
KRH58398	Soybean	plasma membrane, vacuole	41.44	42.37
KRH58389	Soybean	nucleus	41.8	42.35
CDX77574	Canola	cytosol	31.12	42.25
CDY29117	Canola	vacuole	41.62	42.24
VIT_12s0028g01790.t01	Wine grape	plastid	42.36	42.2
KRH36853	Soybean	vacuole	41.62	42.09
Bra020915.1-P	Field mustard	vacuole	41.07	42.0
KRH42269	Soybean	vacuole	41.07	42.0
HORVU7Hr1G089260.1	Barley	mitochondrion	37.57	41.98
VIT_10s0003g05550.t01	Wine grape	vacuole	40.88	41.81
CDY09546	Canola	extracellular, vacuole	41.25	41.79
CDX94009	Canola	vacuole	41.44	41.74
CDX76449	Canola	vacuole	40.88	41.73
KRH11826	Soybean	nucleus	41.8	41.73
KRH58391	Soybean	vacuole	40.7	41.7
KRH11845	Soybean	vacuole	41.07	41.68
VIT_10s0003g05390.t01	Wine grape	extracellular	41.07	41.68
VIT_10s0003g05580.t01	Wine grape	plasma membrane, vacuole	40.7	41.62
CDY02321	Canola	extracellular, vacuole	41.07	41.6
CDY29118	Canola	cytosol, peroxisome, plastid	29.83	41.54
KRH41927	Soybean	nucleus	40.52	41.51
Solyc10g054480.1.1	Tomato	plastid	23.2	41.45
KRH42268	Soybean	vacuole	40.33	41.4
Bra038798.1-P	Field mustard	vacuole	41.07	41.37
CDX67534	Canola	plasma membrane, vacuole	40.88	41.34
KRH58344	Soybean	vacuole	40.33	41.32
KRH58388	Soybean	vacuole	41.62	41.32
CDY72595	Canola	extracellular, vacuole	28.91	41.32
KRH36766	Soybean	vacuole	40.52	41.28
KRH36842	Soybean	vacuole	40.52	41.28
Bra014886.1-P	Field mustard	extracellular	39.96	41.25
Bra016272.1-P	Field mustard	peroxisome, plasma membrane, plastid	25.6	41.25
KRH42267	Soybean	vacuole	39.96	41.18
Bra028530.1-P	Field mustard	plasma membrane, vacuole	40.7	41.15
KRH11827	Soybean	vacuole	40.7	41.15
VIT_10s0003g04730.t01	Wine grape	plasma membrane, vacuole	40.52	41.12
KRH36843	Soybean	vacuole	40.33	41.09
Bra027549.1-P	Field mustard	peroxisome	33.89	41.07
Bra027552.1-P	Field mustard	peroxisome	25.78	41.06
VIT_10s0003g05620.t01	Wine grape	vacuole	40.15	41.05
CDY68515	Canola	extracellular, plasma membrane	28.73	41.05
KRH58399	Soybean	nucleus	40.33	41.01
AT4G20840.1	Thale cress	plasma membrane	40.7	41.0
CDX98676	Canola	vacuole	40.7	41.0
HORVU4Hr1G062830.1	Barley	mitochondrion, peroxisome, plasma membrane	43.83	40.96
Bra027555.1-P	Field mustard	extracellular, vacuole	40.15	40.9
PGSC0003DMT400054564	Potato	cytosol, plastid	37.2	40.89
KRH11824	Soybean	nucleus	40.88	40.88
KRH36839	Soybean	nucleus	40.88	40.88
KRH36840	Soybean	nucleus	41.25	40.88
Bra019831.1-P	Field mustard	plasma membrane, vacuole	39.96	40.87
CDX97984	Canola	plasma membrane, vacuole	39.96	40.87
CDX94788	Canola	vacuole	40.33	40.86
PGSC0003DMT400043091	Potato	extracellular	39.78	40.83
Bra013476.1-P	Field mustard	cytosol, plastid	40.52	40.82
CDY09547	Canola	extracellular, vacuole	39.59	40.8
AT4G20820.1	Thale cress	plastid	39.96	40.79
CDY49803	Canola	vacuole	39.78	40.75
AT5G44410.1	Thale cress	golgi	40.15	40.75
Bra013475.1-P	Field mustard	golgi, vacuole	40.15	40.75
CDX79007	Canola	golgi, vacuole	40.15	40.75
CDX94008	Canola	golgi	40.15	40.75
CDY50728	Canola	extracellular, vacuole	39.41	40.68
AT1G30740.2	Thale cress	vacuole	40.15	40.67
KRH56395	Soybean	nucleus	39.59	40.64
PGSC0003DMT400054571	Potato	cytosol	30.39	40.64
Solyc02g070090.1.1	Tomato	extracellular	39.96	40.64
AT1G30700.1	Thale cress	vacuole	39.41	40.61
AT1G30720.1	Thale cress	vacuole	39.41	40.61
PGSC0003DMT400054385	Potato	plastid	37.02	40.61
CDY13353	Canola	plasma membrane	40.15	40.6
KRH62528	Soybean	endoplasmic reticulum, golgi, vacuole	39.41	40.53
CDY13104	Canola	extracellular, vacuole	39.78	40.53
Bra013473.1-P	Field mustard	vacuole	39.23	40.49
PGSC0003DMT400046308	Potato	extracellular	40.52	40.44
AT1G26390.1	Thale cress	vacuole	39.41	40.38
Bra038799.1-P	Field mustard	golgi	39.78	40.37
HORVU3Hr1G083860.2	Barley	mitochondrion, plastid	24.68	40.36
AT4G20800.1	Thale cress	endoplasmic reticulum, extracellular, vacuole	39.23	40.34
CDY71012	Canola	extracellular, vacuole	39.23	40.34
CDY29116	Canola	extracellular, vacuole	39.59	40.34
AT1G30730.1	Thale cress	extracellular	39.04	40.3
CDX82776	Canola	endoplasmic reticulum, vacuole	39.59	40.26
KRH56394	Soybean	vacuole	39.04	40.23
AT2G34790.1	Thale cress	golgi, vacuole	39.41	40.23
AT5G44400.1	Thale cress	golgi, vacuole	39.78	40.22
AT5G44390.1	Thale cress	vacuole	40.15	40.22
PGSC0003DMT400089776	Potato	golgi, vacuole	40.52	40.22
Solyc02g069690.1.1	Tomato	golgi, vacuole	40.52	40.22
CDY16155	Canola	endoplasmic reticulum, extracellular, vacuole	38.49	40.19
Bra023122.1-P	Field mustard	vacuole	39.23	40.19
CDX98675	Canola	golgi	39.59	40.19
Bra023126.1-P	Field mustard	extracellular, vacuole	39.04	40.15
CDX79005	Canola	vacuole	38.86	40.11
AT1G11770.1	Thale cress	vacuole	39.59	40.11
VIT_10s0003g05420.t01	Wine grape	vacuole	39.78	40.07
Bra033740.1-P	Field mustard	golgi	40.33	40.04
PGSC0003DMT400054387	Potato	plasma membrane, vacuole	39.04	40.0
AT1G01980.1	Thale cress	plasma membrane, vacuole	39.78	39.93
VIT_12s0028g01770.t01	Wine grape	plasma membrane, vacuole	41.8	39.82
KRH42272	Soybean	vacuole	39.41	39.7
Bra013472.1-P	Field mustard	extracellular, vacuole	38.49	39.66
CDX79004	Canola	extracellular, vacuole	38.49	39.66
AT1G26380.1	Thale cress	endoplasmic reticulum	39.04	39.63
AT5G44360.2	Thale cress	golgi	39.96	39.6
PGSC0003DMT400054574	Potato	mitochondrion, peroxisome	37.38	39.57
Solyc02g022850.1.1	Tomato	vacuole	39.41	39.56
VIT_10s0003g05570.t01	Wine grape	plasma membrane, vacuole	40.52	39.5
CDY31642	Canola	plasma membrane	37.02	39.49
HORVU3Hr1G083890.1	Barley	cytosol, peroxisome	25.6	39.49
PGSC0003DMT400054386	Potato	vacuole	38.67	39.47
HORVU7Hr1G026630.1	Barley	plastid	36.83	39.45
CDX79008	Canola	vacuole	41.25	39.44
Solyc02g070110.1.1	Tomato	extracellular, vacuole	38.49	39.43
Solyc02g070130.1.1	Tomato	vacuole	38.67	39.4
Solyc02g070060.1.1	Tomato	vacuole	38.12	39.35
CDY43653	Canola	golgi	22.1	39.34
CDX82782	Canola	vacuole	41.25	39.3
CDX84280	Canola	plasma membrane	36.83	39.29
Bra033743.1-P	Field mustard	vacuole	38.86	39.29
CDY49011	Canola	vacuole	38.86	39.29
VIT_10s0003g05470.t01	Wine grape	vacuole	40.88	39.29
Bra005392.1-P	Field mustard	plasma membrane	38.49	39.29
CDX82775	Canola	extracellular, vacuole	38.12	39.2
Bra006964.1-P	Field mustard	endoplasmic reticulum, extracellular, vacuole	38.67	39.18
KRH41928	Soybean	vacuole	38.31	39.17
Bra023131.1-P	Field mustard	endoplasmic reticulum, plasma membrane, vacuole	38.49	39.14
CDX77568	Canola	endoplasmic reticulum, plasma membrane	38.49	39.14
CDY02326	Canola	endoplasmic reticulum, plasma membrane, vacuole	38.49	39.14
AT1G26420.1	Thale cress	vacuole	38.12	39.13
CDX94785	Canola	vacuole	38.67	39.11
AT1G26400.1	Thale cress	golgi, vacuole	37.94	39.09
CDX82774	Canola	extracellular, vacuole	37.94	39.09
KRH11830	Soybean	vacuole	35.36	39.02
KRH11841	Soybean	vacuole	40.15	39.0
CDX85019	Canola	extracellular	37.2	39.0
CDY67309	Canola	endoplasmic reticulum, plasma membrane	38.31	38.95
KRH49946	Soybean	extracellular, vacuole	30.39	38.92
Bra024717.1-P	Field mustard	vacuole	37.75	38.9
CDY16156	Canola	vacuole	37.75	38.9
VIT_03s0017g01490.t01	Wine grape	vacuole	39.04	38.9
PGSC0003DMT400054560	Potato	cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	25.97	38.84
Bra014885.1-P	Field mustard	plasma membrane, vacuole	37.94	38.79
Solyc02g070070.1.1	Tomato	extracellular	37.94	38.79
Bra022992.1-P	Field mustard	mitochondrion	34.07	38.78
CDY70640	Canola	mitochondrion	34.07	38.78
PGSC0003DMT400086243	Potato	vacuole	37.57	38.78
Bra014890.1-P	Field mustard	plasma membrane	38.12	38.76
CDY34627	Canola	vacuole	38.12	38.69
Solyc02g070170.1.1	Tomato	extracellular, vacuole	38.12	38.69
CDY70228	Canola	cytosol, golgi, peroxisome	21.92	38.64
Bra027553.1-P	Field mustard	vacuole	30.94	38.62
AT1G30710.1	Thale cress	extracellular, plasma membrane, vacuole	37.75	38.61
CDX79582	Canola	mitochondrion	33.89	38.57
AT1G34575.1	Thale cress	vacuole	37.38	38.52
Bra027547.1-P	Field mustard	vacuole	38.31	38.52
Bra031780.1-P	Field mustard	vacuole	38.12	38.48
CDY34829	Canola	vacuole	38.12	38.48
KRH42271	Soybean	endoplasmic reticulum	40.88	38.47
CDX77576	Canola	plasma membrane, vacuole	37.57	38.42
CDY50727	Canola	plasma membrane, vacuole	37.57	38.42
VIT_03s0017g01420.t01	Wine grape	cytosol, peroxisome, vacuole	39.04	38.41
CDY13105	Canola	vacuole	38.12	38.33
KRH11825	Soybean	vacuole	36.83	38.31
Solyc02g079440.1.1	Tomato	nucleus	37.94	38.29
CDY49007	Canola	vacuole	38.12	38.26
PGSC0003DMT400054393	Potato	plasma membrane, vacuole	37.57	38.2
PGSC0003DMT400012979	Potato	cytosol	27.99	38.19
PGSC0003DMT400057528	Potato	extracellular	37.75	38.1
Solyc02g070200.1.1	Tomato	vacuole	37.94	38.08
AT4G20830.1	Thale cress	vacuole	39.96	38.07
Solyc09g072660.2.1	Tomato	extracellular	38.49	38.07
KRH36851	Soybean	vacuole	39.59	38.05
CDX94780	Canola	plasma membrane, vacuole	37.94	37.94
AT4G20860.1	Thale cress	vacuole	37.02	37.92
CDY68914	Canola	golgi, plastid	30.2	37.88
CDY29122	Canola	cytosol	13.44	37.82
CDX79034	Canola	plastid	36.28	37.81
PGSC0003DMT400062414	Potato	plasma membrane, vacuole	38.49	37.73
VIT_10s0003g05450.t01	Wine grape	extracellular	39.78	37.63
Bra013501.1-P	Field mustard	vacuole	36.83	37.59
AT2G34810.1	Thale cress	vacuole	37.38	37.59
Bra023123.1-P	Field mustard	plasma membrane, vacuole	35.36	37.5
CDY02318	Canola	plasma membrane, vacuole	35.36	37.5
KRH58395	Soybean	endoplasmic reticulum	40.15	37.46
AT1G30760.2	Thale cress	golgi	37.94	37.32
Bra005390.1-P	Field mustard	vacuole	37.02	37.29
CDY69038	Canola	vacuole	37.02	37.29
CDX94786	Canola	vacuole	36.46	37.29
HORVU3Hr1G083840.1	Barley	mitochondrion, plastid	37.57	37.23
Solyc02g070180.1.1	Tomato	plasma membrane, vacuole	37.02	37.22
Bra013478.1-P	Field mustard	endoplasmic reticulum, vacuole	35.73	37.16
CDX82785	Canola	endoplasmic reticulum, extracellular, vacuole	35.73	37.16
CDX82800	Canola	extracellular	35.73	37.16
KRH36841	Soybean	vacuole	26.89	37.15
Solyc02g079450.1.1	Tomato	extracellular	36.65	37.06
AT1G26410.1	Thale cress	golgi	37.57	36.96
KRH42276	Soybean	nucleus	40.33	36.93
CDX82780	Canola	cytosol	14.55	36.92
KRH12123	Soybean	plastid	31.12	36.9
PGSC0003DMT400057680	Potato	extracellular, plasma membrane, vacuole	36.46	36.87
Bra021930.1-P	Field mustard	plasma membrane	34.07	36.85
Solyc02g079470.1.1	Tomato	endoplasmic reticulum, vacuole	36.46	36.73
CDY52527	Canola	vacuole	36.65	36.72
Solyc02g079460.1.1	Tomato	extracellular, golgi, vacuole	35.91	36.65
CDY69774	Canola	endoplasmic reticulum, extracellular, plastid, vacuole	35.17	36.59
KRH71761	Soybean	mitochondrion, plastid, vacuole	28.55	36.47
AT5G44440.2	Thale cress	golgi	40.33	36.32
AT5G44380.2	Thale cress	mitochondrion, plastid	38.67	36.14
PGSC0003DMT400036039	Potato	extracellular, plasma membrane, vacuole	36.83	36.1
CDX79010	Canola	cytosol	17.13	34.83
CDY70679	Canola	cytosol, mitochondrion, peroxisome	14.92	34.76
HORVU1Hr1G091500.1	Barley	extracellular, vacuole	35.17	33.57
PGSC0003DMT400057523	Potato	extracellular, golgi, vacuole	34.25	33.04
Bra033739.1-P	Field mustard	plasma membrane, vacuole	36.83	32.89
Solyc02g079480.1.1	Tomato	plasma membrane, vacuole	33.52	32.44
CDX85018	Canola	cytosol	39.04	31.64
KRG99206	Soybean	extracellular, peroxisome	21.92	29.46

Protein Annotations

Gene3D:3.30.43.10	Gene3D:3.30.465.50	Gene3D:3.40.462.20	MapMan:50.1.1	UniProt:A0A287H0P1	InterPro:BBE
InterPro:FAD-bd_2	InterPro:FAD-bd_2-like_sf	InterPro:FAD-bd_2_sub1	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824
GO:GO:0005488	GO:GO:0008150	GO:GO:0008152	GO:GO:0016491	GO:GO:0016614	GO:GO:0050660
GO:GO:0055114	GO:GO:0071949	EnsemblPlantsGene:HORVU2Hr1G008310	EnsemblPlants:HORVU2Hr1G008310.1	InterPro:IPR016166	InterPro:IPR016167
InterPro:Oxid_FAD_bind_N	PFAM:PF01565	PFAM:PF08031	PFscan:PS51387	PANTHER:PTHR32448	PANTHER:PTHR32448:SF32
SUPFAM:SSF56176	SignalP:SignalP-noTM	TMHMM:TMhelix	UniParc:UPI000B4767D6	SEG:seg	:

Description

No Description!

Coordinates

chrchr2H:-:17133219..17135208

Molecular Weight (calculated)

58528.9 Da

IEP (calculated)

8.206

GRAVY (calculated)

-0.091

Length

543 amino acids

Sequence
(BLAST)

001: EAPRRTADPS KMASISLVVI ICVLGFFVAA PSQASPSDHS DNDNDFLRCL STDVPGQLVQ APSSPSFTPL LVSSIRNARL VAPATSRPPI CIVTPTNASH
101: VQAAVRCGRR HAVRVRVRSG GHDCEGLSYR SGSPKSEAFA VIDLANLHAV TVDPHEATAW VDSGAGIGEL YYHVATAAPG LGFPAGVCPT IGVGGHFSGG
201: GMGLMMRKHG LSADNVLDAT MVDAQGNLLA DKKAMGDDLF WAIRGGGGGN FGIVLSWKVR LVPVPPTVTF FKVPKTMEQG AVDAVTKWQT LAPALPDDLS
301: VRVVVQNREA NFQALHLGNC SAAVATMGSR FPELGVTTAD CKEMSWLNYT AYIFFGDAIN AKPLEALLLN RSMPLGRFVK NKSDYVKKPL TKETWDKIFQ
401: WPNGTATGQL VMEPHGGIMA RISADGIPFP HRSSVLYNIQ YVEFWNGTAA GGDVTPKWVG SLYEFMAPFV SKNPRGAYVN YRDLDIGTNK VVDGVTSYES
501: GKVWGESYFG PENYRRLAKI KRTVDADDYF RSEQSMPPLS LSQ

Best Arabidopsis Sequence Match ( AT4G20820.1 )
(BLAST)

001: MKFQSFFSSV LIFFTTSTLL LSIPHPVSAN RSNQSSFLQC LSLQLNDSNI VSKVIHTPND TSFSSVLASS IQNQRFSAPD VPKPVLILTP VQPSDVQSAV
101: KCARRFGIHI RTRSGGHDYE GLSYVTHKPF VILDLRNLRS ITVDVDNRSV WVQTGATIGE LYYEIGKKNR TLAFPAGVCP TVGVGGHFSG GGYGTLLRKH
201: GLAADHVIDA RVVDARGRIL ERREMGEDFF WAIRGGGGSS FCVVLSWKIG LINVPSTVTV FNVTKFSEQS ALKIIHRWQF VADKVSDDLF IRVMLQRYKN
301: MVRASFPGLY LGSVKNLLKM VNKEFPELGL EEDDCTEMSW IESVIWFAEL GEEPINVLTK RTRASLAFKA KSDFVQEPMP KTAISKLWRR LQEPEAEHAQ
401: LIFTPFGGKM SEIADYETPF PHRKGNIYEI QYLNYWRGDV KEKYMRWVER VYDDMSEFVA KSPRGAYINL RDLDLGMYVG VKRSKYEEGK SWGVKYFKNN
501: FERLVRVKTS VDPSDFFCDE QSIPPFTFVE VI

Arabidopsis Description

Berberine bridge enzyme-like 18 [Source:UniProtKB/Swiss-Prot;Acc:Q9SVG5]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.