CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 2
cytosol 3
mitochondrion 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, mitochondrion, nucleus
BaCelLo:	nucleus
EpiLoc:	cytosol
MultiLoc:	cytosol
Plant-mPloc:	mitochondrion
PProwler:	mitochondrion
WoLF PSORT:	nucleus
YLoc:	cytosol

nucleus: 21132161
endoplasmic reticulum: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH03376
KRH54462
KRH57009
KRH63457

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G44110.1	KRH03376	AT3G48100.1	18642946
AT3G44110.1	KRH54462	AT3G48100.1	18642946
AT3G44110.1	KRH57009	AT3G48100.1	18642946
AT3G44110.1	KRH63457	AT3G48100.1	18642946

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRG88903	Soybean	nucleus	99.04	99.04
KRH22611	Soybean	nucleus	93.05	93.05
KRH26709	Soybean	nucleus	92.33	92.33
VIT_06s0080g01230.t01	Wine grape	cytosol	89.93	89.93
GSMUA_Achr4P22790_001	Banana	cytosol	89.21	89.0
PGSC0003DMT400060145	Potato	cytosol	89.21	88.78
Solyc05g055160.2.1	Tomato	nucleus	89.21	88.78
KXG37403	Sorghum	cytosol	88.97	88.76
GSMUA_Achr4P31390_001	Banana	cytosol	88.49	88.7
PGSC0003DMT400005019	Potato	cytosol	88.97	88.54
Solyc11g006460.1.1	Tomato	nucleus	88.73	88.31
Zm00001d034368_P001	Maize	extracellular, plasma membrane	88.49	88.28
Os03t0787300-01	Rice	plasma membrane	88.01	88.01
Zm00001d013111_P001	Maize	extracellular, plasma membrane	87.77	87.77
GSMUA_Achr6P29960_001	Banana	nucleus	86.81	87.44
EER93745	Sorghum	nucleus	87.77	87.35
Os03t0648400-02	Rice	nucleus	87.05	87.05
Zm00001d033210_P002	Maize	nucleus	87.29	86.87
GSMUA_Achr9P25940_001	Banana	nucleus	86.33	86.33
AT3G44110.1	Thale cress	nucleus	86.57	85.95
HORVU5Hr1G103680.1	Barley	cytosol	86.57	85.75
TraesCS5A01G426100.1	Wheat	nucleus	86.57	85.75
Solyc04g009770.2.1	Tomato	nucleus	85.85	85.65
GSMUA_Achr7P01290_001	Banana	nucleus	85.61	85.61
TraesCS5B01G428000.1	Wheat	nucleus	86.33	85.51
TraesCS5D01G434100.1	Wheat	nucleus	86.33	85.51
PGSC0003DMT400065401	Potato	nucleus	85.13	84.73
CDX92386	Canola	nucleus	85.13	84.12
Bra002385.1-P	Field mustard	nucleus	85.13	84.12
CDX92385	Canola	nucleus	84.89	83.89
PGSC0003DMT400007728	Potato	nucleus	84.17	83.77
Bra002386.1-P	Field mustard	nucleus	84.65	83.65
CDY09117	Canola	nucleus	84.65	83.45
CDY65546	Canola	nucleus	84.17	83.37
Bra020169.1-P	Field mustard	nucleus	84.17	82.98
AT5G22060.1	Thale cress	cytosol	82.97	82.58
Solyc11g071830.1.1	Tomato	plastid	81.77	81.58
Zm00001d013669_P002	Maize	cytosol	87.53	81.29
Zm00001d006666_P001	Maize	nucleus	56.83	80.89
Zm00001d048538_P001	Maize	nucleus	62.59	80.31
Bra006608.1-P	Field mustard	nucleus	77.7	79.41
CDX88812	Canola	nucleus	77.7	79.41
CDX70980	Canola	nucleus	76.74	78.62
Zm00001d006230_P001	Maize	cytosol	53.24	76.55
Zm00001d045645_P001	Maize	cytosol	48.44	75.94
Zm00001d031357_P001	Maize	cytosol, nucleus, plastid	33.33	75.54
CDY16617	Canola	nucleus	37.65	75.48
KRH48754	Soybean	endoplasmic reticulum	74.82	74.29
KRH66593	Soybean	endoplasmic reticulum	74.58	74.05
HORVU6Hr1G065410.3	Barley	cytosol, mitochondrion	50.12	71.58
Zm00001d006563_P001	Maize	mitochondrion	46.04	70.59
Zm00001d026185_P001	Maize	endoplasmic reticulum	80.1	66.4
Zm00001d004840_P001	Maize	mitochondrion	53.24	64.72
KRH69388	Soybean	cytosol	25.66	38.77
KRH61858	Soybean	cytosol	12.95	38.03
KRH61857	Soybean	cytosol, nucleus	10.55	37.93
KRH74747	Soybean	cytosol	24.94	37.55
KRH45833	Soybean	cytosol	25.9	37.37
KRH10937	Soybean	cytosol	29.98	36.87
KRH00241	Soybean	cytosol, nucleus	29.98	36.87
KRG88624	Soybean	nucleus	29.74	36.8
KRG96505	Soybean	nucleus	30.22	36.73
KRG99186	Soybean	cytosol	25.42	36.68
KRH52529	Soybean	cytosol	28.54	36.5
KRH65726	Soybean	nucleus	29.5	36.5
KRH75968	Soybean	nucleus	29.26	36.2
KRH49388	Soybean	cytosol	29.98	36.13
KRH68242	Soybean	endoplasmic reticulum	29.5	35.86
KRH25647	Soybean	nucleus	29.98	35.82
KRH63415	Soybean	cytosol	12.47	35.37
KRH55895	Soybean	endoplasmic reticulum, nucleus	29.5	34.94
KRH40259	Soybean	cytosol	13.19	34.59
KRH10940	Soybean	cytosol	27.82	34.52
KRH54495	Soybean	nucleus	29.02	34.47
KRH63417	Soybean	nucleus	16.55	33.99
KRH21362	Soybean	cytosol	27.58	33.82
KRH69236	Soybean	endoplasmic reticulum	28.06	33.81
KRH21363	Soybean	cytosol	24.7	33.44
KRH12423	Soybean	cytoskeleton, cytosol, nucleus	19.9	31.56
KRH60063	Soybean	endoplasmic reticulum, golgi	13.43	28.28
KRH55068	Soybean	cytosol	8.63	26.28
KRG97564	Soybean	plastid	26.38	25.0
KRH43163	Soybean	plastid	26.14	24.94
KRH59311	Soybean	plastid	25.9	24.94
KRH31310	Soybean	nucleus, plastid	26.14	24.77
KRH27511	Soybean	mitochondrion	25.9	24.38
KRH13903	Soybean	mitochondrion	25.9	24.32
KRH36456	Soybean	mitochondrion	25.42	23.93
KRH43470	Soybean	mitochondrion	25.42	23.87
KRH44477	Soybean	plastid	24.94	23.42
KRH10414	Soybean	cytosol	19.66	21.52
KRH09715	Soybean	nucleus, plastid	25.42	21.5
KRH48662	Soybean	nucleus, plastid	26.38	20.95
KRH16800	Soybean	mitochondrion	23.02	20.47
KRH44054	Soybean	cytosol	18.7	20.31
KRH72418	Soybean	mitochondrion	22.78	20.26
KRH64663	Soybean	plastid	16.55	19.49
KRH23590	Soybean	nucleus	15.59	18.21
KRG90292	Soybean	cytosol, nucleus, plastid	11.51	16.78

Protein Annotations

Gene3D:1.10.287.110	MapMan:19.1.5.2	Gene3D:2.10.230.10	Gene3D:2.60.260.20	EntrezGene:547558	EMBL:ACUP02007237
EMBL:AF169022	InterPro:DnaJ	InterPro:DnaJ_C	InterPro:DnaJ_domain	InterPro:DnaJ_domain_CS	EnsemblPlantsGene:GLYMA_12G095700
GO:GO:0000166	GO:GO:0003674	GO:GO:0005488	GO:GO:0005515	GO:GO:0005524	GO:GO:0006457
GO:GO:0006950	GO:GO:0008150	GO:GO:0009408	GO:GO:0009628	GO:GO:0009987	GO:GO:0031072
GO:GO:0046872	GO:GO:0051082	InterPro:HSP40/DnaJ_pept-bd	InterPro:HSP_DnaJ_Cys-rich_dom	InterPro:HSP_DnaJ_Cys-rich_dom_sf	InterPro:IPR001305
InterPro:IPR001623	InterPro:IPR036869	InterPro:J_dom_sf	EnsemblPlants:KRH25334	ProteinID:KRH25334	ProteinID:KRH25334.1
HAMAP:MF_01152	PFAM:PF00226	PFAM:PF00684	PFAM:PF01556	PRINTS:PR00625	ScanProsite:PS00636
PFscan:PS50076	PFscan:PS51188	PANTHER:PTHR43888	PANTHER:PTHR43888:SF4	UniProt:Q9SWB5	SMART:SM00271
SUPFAM:SSF46565	SUPFAM:SSF49493	SUPFAM:SSF57938	UniParc:UPI000009FFE1	SEG:seg	:

Description

hypothetical protein

Coordinates

chr12:+:8033041..8036166

Molecular Weight (calculated)

46303.8 Da

IEP (calculated)

6.205

GRAVY (calculated)

-0.762

Length

417 amino acids

Sequence
(BLAST)

001: MFGRAPKKSD NTRYYEILGV SKNASQDDLK KAYKKAAIKN HPDKGGDPEK FKELAQAYEV LSDPEKREIY DQYGEDALKE GMGGGGGHDP FDIFSSFFGG
101: GSPFGSGGSS RGRRQRRGED VVHPLKVSLE DLYLGTSKKL SLSRNVICSK CSGKGSKSGA SMKCAGCQGT GMKVSIRHLG PSMIQQMQHA CNECKGTGET
201: INDRDRCPQC KGEKVVQEKK VLEVIVEKGM QNGQKITFPG EADEAPDTIT GDIVFVLQQK EHPKFKRKAE DLFVEHTLSL TEALCGFQFV LTHLDSRQLL
301: IKSNPGEVVK PDSYKAINDE GMPMYQRPFM KGKLYIHFTV EFPDSLNPDQ VKALEAVLPP KPSSQLTDME LDECEETTLH DVNMEEETRR KQQQAQEAYD
401: EDDDMPGGAQ RVQCAQQ

Best Arabidopsis Sequence Match ( AT5G22060.1 )
(BLAST)

001: MFGRGPSRKS DNTKFYEILG VPKTAAPEDL KKAYKKAAIK NHPDKGGDPE KFKELAQAYE VLSDPEKREI YDQYGEDALK EGMGGGGGGH DPFDIFSSFF
101: GSGGHPFGSH SRGRRQRRGE DVVHPLKVSL EDVYLGTTKK LSLSRKALCS KCNGKGSKSG ASMKCGGCQG SGMKISIRQF GPGMMQQVQH ACNDCKGTGE
201: TINDRDRCPQ CKGEKVVSEK KVLEVNVEKG MQHNQKITFS GQADEAPDTV TGDIVFVIQQ KEHPKFKRKG EDLFVEHTIS LTEALCGFQF VLTHLDKRQL
301: LIKSKPGEVV KPDSYKAISD EGMPIYQRPF MKGKLYIHFT VEFPESLSPD QTKAIEAVLP KPTKAAISDM EIDDCEETTL HDVNIEDEMK RKAQAQREAY
401: DDDEEDHPGG AQRVQCAQQ

Arabidopsis Description

ATJ2At5g22060 [Source:UniProtKB/TrEMBL;Acc:Q0V7U1]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.