CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 2
cytosol 3
mitochondrion 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, mitochondrion, nucleus
BaCelLo:	nucleus
EpiLoc:	cytosol
MultiLoc:	cytosol
Plant-mPloc:	mitochondrion
PProwler:	mitochondrion
WoLF PSORT:	nucleus
YLoc:	cytosol

nucleus: 21132161
endoplasmic reticulum: 23041469
endoplasmic reticulum: 27224218
nucleus: 27291164
mitochondrion: 27780359
endoplasmic reticulum: 28499913
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27780359 doi

G Mustafa, S Komatsu

Graduate School of Life and Environmental Science, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 23041469 doi

S Komatsu, R Kuji, Y Nanjo, S Hiraga, K Furukawa

National Institute of Crop Science, Tsukuba 305-8518, Japan. skomatsu@affrc.go.jp

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27291164 doi

X Yin, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 27224218 doi

X Wang, S Komatsu

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH03376
KRH54462
KRH57009
KRH63457

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G44110.1	KRH03376	AT3G48100.1	18642946
AT3G44110.1	KRH54462	AT3G48100.1	18642946
AT3G44110.1	KRH57009	AT3G48100.1	18642946
AT3G44110.1	KRH63457	AT3G48100.1	18642946

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH22611	Soybean	nucleus	97.6	97.6
KRH25334	Soybean	nucleus	92.33	92.33
KRG88903	Soybean	nucleus	92.33	92.33
VIT_06s0080g01230.t01	Wine grape	cytosol	88.73	88.73
KXG37403	Sorghum	cytosol	88.25	88.04
PGSC0003DMT400060145	Potato	cytosol	88.25	87.83
Solyc05g055160.2.1	Tomato	nucleus	88.25	87.83
Zm00001d034368_P001	Maize	extracellular, plasma membrane	87.05	86.84
Os03t0787300-01	Rice	plasma membrane	86.81	86.81
Zm00001d013111_P001	Maize	extracellular, plasma membrane	86.33	86.33
GSMUA_Achr4P31390_001	Banana	cytosol	85.37	85.58
HORVU5Hr1G103680.1	Barley	cytosol	86.33	85.51
TraesCS5B01G428000.1	Wheat	nucleus	86.33	85.51
TraesCS5D01G434100.1	Wheat	nucleus	86.33	85.51
GSMUA_Achr4P22790_001	Banana	cytosol	85.61	85.41
TraesCS5A01G426100.1	Wheat	nucleus	86.09	85.27
Solyc11g006460.1.1	Tomato	nucleus	85.13	84.73
PGSC0003DMT400005019	Potato	cytosol	85.13	84.73
GSMUA_Achr9P25940_001	Banana	nucleus	84.41	84.41
GSMUA_Achr6P29960_001	Banana	nucleus	83.45	84.06
Solyc04g009770.2.1	Tomato	nucleus	84.17	83.97
GSMUA_Achr7P01290_001	Banana	nucleus	83.93	83.93
Zm00001d033210_P002	Maize	nucleus	83.93	83.53
EER93745	Sorghum	nucleus	83.93	83.53
Os03t0648400-02	Rice	nucleus	83.45	83.45
AT3G44110.1	Thale cress	nucleus	83.69	83.1
PGSC0003DMT400065401	Potato	nucleus	83.21	82.82
PGSC0003DMT400007728	Potato	nucleus	82.49	82.1
CDX92386	Canola	nucleus	82.97	81.99
Bra002385.1-P	Field mustard	nucleus	82.97	81.99
CDX92385	Canola	nucleus	82.73	81.75
CDY09117	Canola	nucleus	82.73	81.56
Bra002386.1-P	Field mustard	nucleus	82.49	81.52
CDY65546	Canola	nucleus	82.25	81.47
Solyc11g071830.1.1	Tomato	plastid	81.53	81.34
Bra020169.1-P	Field mustard	nucleus	82.25	81.09
AT5G22060.1	Thale cress	cytosol	80.34	79.95
Zm00001d006666_P001	Maize	nucleus	56.12	79.86
Zm00001d013669_P002	Maize	cytosol	83.93	77.95
Zm00001d048538_P001	Maize	nucleus	60.67	77.85
Bra006608.1-P	Field mustard	nucleus	75.54	77.21
CDX88812	Canola	nucleus	75.54	77.21
CDX70980	Canola	nucleus	74.1	75.92
Zm00001d031357_P001	Maize	cytosol, nucleus, plastid	33.33	75.54
Zm00001d006230_P001	Maize	cytosol	51.56	74.14
Zm00001d045645_P001	Maize	cytosol	47.0	73.68
HORVU6Hr1G065410.3	Barley	cytosol, mitochondrion	50.36	71.92
KRH48754	Soybean	endoplasmic reticulum	71.94	71.43
KRH66593	Soybean	endoplasmic reticulum	71.94	71.43
CDY16617	Canola	nucleus	35.25	70.67
Zm00001d006563_P001	Maize	mitochondrion	45.32	69.49
Zm00001d026185_P001	Maize	endoplasmic reticulum	78.9	65.41
Zm00001d004840_P001	Maize	mitochondrion	52.04	63.27
KRH69388	Soybean	cytosol	25.18	38.04
KRH61857	Soybean	cytosol, nucleus	10.55	37.93
KRH74747	Soybean	cytosol	24.94	37.55
KRH61858	Soybean	cytosol	12.71	37.32
KRG96505	Soybean	nucleus	30.7	37.32
KRH45833	Soybean	cytosol	25.42	36.68
KRH10937	Soybean	cytosol	29.74	36.58
KRH68242	Soybean	endoplasmic reticulum	29.98	36.44
KRH52529	Soybean	cytosol	28.3	36.2
KRH00241	Soybean	cytosol, nucleus	29.26	35.99
KRG99186	Soybean	cytosol	24.94	35.99
KRG88624	Soybean	nucleus	29.02	35.91
KRH65726	Soybean	nucleus	29.02	35.91
KRH25647	Soybean	nucleus	29.74	35.53
KRH75968	Soybean	nucleus	28.54	35.31
KRH49388	Soybean	cytosol	29.26	35.26
KRH55895	Soybean	endoplasmic reticulum, nucleus	29.26	34.66
KRH69236	Soybean	endoplasmic reticulum	28.3	34.1
KRH63417	Soybean	nucleus	16.55	33.99
KRH10940	Soybean	cytosol	27.34	33.93
KRH21363	Soybean	cytosol	24.7	33.44
KRH40259	Soybean	cytosol	12.71	33.33
KRH21362	Soybean	cytosol	27.1	33.24
KRH54495	Soybean	nucleus	27.82	33.05
KRH63415	Soybean	cytosol	11.27	31.97
KRH12423	Soybean	cytoskeleton, cytosol, nucleus	19.66	31.18
KRH60063	Soybean	endoplasmic reticulum, golgi	12.95	27.27
KRG97564	Soybean	plastid	27.34	25.91
KRH31310	Soybean	nucleus, plastid	27.1	25.68
KRH59311	Soybean	plastid	26.62	25.64
KRH43163	Soybean	plastid	26.86	25.63
KRH55068	Soybean	cytosol	8.39	25.55
KRH27511	Soybean	mitochondrion	26.62	25.06
KRH13903	Soybean	mitochondrion	26.38	24.77
KRH36456	Soybean	mitochondrion	26.14	24.61
KRH43470	Soybean	mitochondrion	25.9	24.32
KRH44477	Soybean	plastid	24.46	22.97
KRH10414	Soybean	cytosol	20.38	22.31
KRH09715	Soybean	nucleus, plastid	25.42	21.5
KRH48662	Soybean	nucleus, plastid	26.62	21.14
KRH16800	Soybean	mitochondrion	23.26	20.68
KRH44054	Soybean	cytosol	18.94	20.57
KRH72418	Soybean	mitochondrion	23.02	20.47
KRH64663	Soybean	plastid	17.03	20.06
KRH23590	Soybean	nucleus	15.59	18.21
KRG90292	Soybean	cytosol, nucleus, plastid	11.75	17.13

Protein Annotations

Gene3D:1.10.287.110	EntrezGene:100778677	MapMan:19.1.5.2	Gene3D:2.10.230.10	Gene3D:2.60.260.20	EMBL:ACUP02007744
ncoils:Coil	InterPro:DnaJ	InterPro:DnaJ_C	InterPro:DnaJ_domain	InterPro:DnaJ_domain_CS	EnsemblPlantsGene:GLYMA_12G190100
GO:GO:0000166	GO:GO:0003674	GO:GO:0005488	GO:GO:0005515	GO:GO:0005524	GO:GO:0006457
GO:GO:0006950	GO:GO:0008150	GO:GO:0009408	GO:GO:0009628	GO:GO:0009987	GO:GO:0031072
GO:GO:0046872	GO:GO:0051082	InterPro:HSP40/DnaJ_pept-bd	InterPro:HSP_DnaJ_Cys-rich_dom	InterPro:HSP_DnaJ_Cys-rich_dom_sf	UniProt:I1LU10
InterPro:IPR001305	InterPro:IPR001623	InterPro:IPR036869	InterPro:J_dom_sf	EnsemblPlants:KRH26709	ProteinID:KRH26709
ProteinID:KRH26709.1	HAMAP:MF_01152	PFAM:PF00226	PFAM:PF00684	PFAM:PF01556	PRINTS:PR00625
ScanProsite:PS00636	PFscan:PS50076	PFscan:PS51188	PANTHER:PTHR43888	PANTHER:PTHR43888:SF4	SMART:SM00271
SUPFAM:SSF46565	SUPFAM:SSF49493	SUPFAM:SSF57938	UniParc:UPI000233D8AC	SEG:seg	:

Description

hypothetical protein

Coordinates

chr12:+:35168889..35171299

Molecular Weight (calculated)

46337.8 Da

IEP (calculated)

6.250

GRAVY (calculated)

-0.742

Length

417 amino acids

Sequence
(BLAST)

001: MFGRAPKKSD STRYYEILGV SKNASPDDLK KAYKKAAIKN HPDKGGDPEK FKELAQAYEV LSDPEKREIY DTYGEDALKE GMGGGGGGHD PFDIFSSFFG
101: GSPFGSGGSS RGRRQRRGED VVHPLKVSLE DLYLGTSKKL SLSRNVLCSK CNGKGSKSGA SMTCAGCQGT GMKVSIRHLG PSMIQQMQHP CNECKGTGET
201: INDRDRCQQC KGEKVVQEKK VLEVVVEKGM QNGQKITFPG EADEAPDTVT GDIVFVLQQK EHPKFKRKAD DLFVEHTLSL TEALCGFQFV LAHLDGRQLL
301: IKSNPGEVVK PDSYKAINDE GMPNYQRHFL KGKLYIHFSV EFPDTLSLDQ VKALETTLPL KPTSQLTDME LDECEETTLH DVNMEEEIRR RQQAQQEAYE
401: EDEDMHGGAQ RVQCAQQ

Best Arabidopsis Sequence Match ( AT3G44110.1 )
(BLAST)

001: MFGRGPSKKS DNTKFYEILG VPKSASPEDL KKAYKKAAIK NHPDKGGDPE KFKELAQAYE VLSDPEKREI YDQYGEDALK EGMGGGGGGH DPFDIFSSFF
101: GGGPFGGNTS RQRRQRRGED VVHPLKVSLE DVYLGTMKKL SLSRNALCSK CNGKGSKSGA SLKCGGCQGS GMKVSIRQLG PGMIQQMQHA CNECKGTGET
201: INDRDRCPQC KGDKVIPEKK VLEVNVEKGM QHSQKITFEG QADEAPDTVT GDIVFVLQQK EHPKFKRKGE DLFVEHTLSL TEALCGFQFV LTHLDGRSLL
301: IKSNPGEVVK PDSYKAISDE GMPIYQRPFM KGKLYIHFTV EFPDSLSPDQ TKALEAVLPK PSTAQLSDME IDECEETTLH DVNIEDEMRR KAQAQREAYD
401: DDDEDDDHPG GAQRVQCAQQ

Arabidopsis Description

ATJ3DnaJ protein homolog atj3 [Source:UniProtKB/TrEMBL;Acc:Q0WW92]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.