CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 4
mitochondrion 2
cytosol 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG90774
KRG95373
KRH03258
KRH35987
KRH42233
KRH57096
KRH58350
KRH67057
KRH67059
KRH71867

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G53060.1	KRH42233	AT3G60360.1	21798944
AT5G53060.1	KRH58350	AT3G60360.1	21798944
AT5G53060.1	KRH03258	AT4G24680.1	21798944
AT5G53060.1	KRH57096	AT4G24680.1	21798944
AT5G53060.1	KRG90774	AT5G04280.1	21798944
AT5G53060.1	KRH35987	AT5G04280.1	21798944
AT5G53060.1	KRG95373	AT5G06770.1	21798944
AT5G53060.1	KRH67057	AT5G06770.1	21798944
AT5G53060.1	KRH67059	AT5G06770.1	21798944
AT5G53060.1	KRH71867	AT5G06770.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH53532	Soybean	nucleus	95.96	95.52
VIT_16s0098g01660.t01	Wine grape	nucleus	71.27	69.44
Solyc03g082980.2.1	Tomato	nucleus	68.32	65.87
AT5G53060.1	Thale cress	nucleus	64.29	63.5
PGSC0003DMT400047665	Potato	nucleus	67.55	63.41
CDX96185	Canola	nucleus	63.04	63.34
Bra003085.1-P	Field mustard	nucleus	61.96	62.44
CDY56908	Canola	nucleus	60.87	61.64
CDY49453	Canola	nucleus	61.34	61.53
Bra022624.1-P	Field mustard	nucleus	62.89	61.27
CDY27030	Canola	nucleus	63.04	61.05
GSMUA_Achr2P15270_001	Banana	nucleus	58.7	54.39
HORVU7Hr1G082230.1	Barley	nucleus	49.53	50.96
Os06t0342500-01	Rice	cytosol	50.31	48.94
TraesCS7A01G333800.1	Wheat	nucleus	50.47	48.65
TraesCS7D01G341400.1	Wheat	nucleus	50.16	47.99
Zm00001d046033_P001	Maize	nucleus	49.53	47.83
KXG20038	Sorghum	nucleus	49.22	47.46
TraesCS7B01G245400.2	Wheat	nucleus	50.16	45.49
Zm00001d037055_P003	Maize	nucleus	48.14	41.89
KRH42077	Soybean	plastid	26.86	29.47
KRH49838	Soybean	plastid	26.4	28.81
KRG93559	Soybean	nucleus	23.29	27.37
KRH40065	Soybean	nucleus	22.67	27.04
KRH58152	Soybean	cytosol	5.28	26.77
KRH18467	Soybean	nucleus	22.67	26.64
KRH52837	Soybean	plastid	27.64	26.33
KRH01196	Soybean	nucleus	22.05	26.15
KRH47290	Soybean	mitochondrion, nucleus	22.67	26.12
KRH58669	Soybean	endoplasmic reticulum, nucleus	25.16	26.09
KRH42565	Soybean	nucleus	25.31	26.08
KRH21739	Soybean	plastid	28.57	25.73
KRH44614	Soybean	mitochondrion, nucleus	22.36	25.49
KRH62129	Soybean	plastid	26.71	25.37
KRH10613	Soybean	plastid	27.64	24.48
KRH39475	Soybean	plastid	22.36	23.57
KRH00973	Soybean	nucleus, plastid	12.42	22.1
KRH74462	Soybean	nucleus	20.03	20.94
KRH40252	Soybean	cytosol, golgi, nucleus, plastid	12.58	20.88
KRH68752	Soybean	cytoskeleton, cytosol, nucleus	13.98	20.32
KRG97000	Soybean	nucleus	13.51	19.51
KRH19104	Soybean	nucleus	12.42	18.35
KRH02776	Soybean	nucleus	12.42	18.35
KRH12329	Soybean	cytosol, nucleus	12.11	17.65
KRH37335	Soybean	cytosol, nucleus	12.11	17.61
KRH67322	Soybean	nucleus	12.73	15.5
KRH71306	Soybean	nucleus	11.65	15.15
KRG95633	Soybean	nucleus	12.58	15.08
KRH32136	Soybean	cytosol	12.42	14.16

Protein Annotations

EntrezGene:100804167	Gene3D:3.30.1370.10	Gene3D:3.30.310.210	MapMan:35.1	EMBL:ACUP02002765	EnsemblPlantsGene:GLYMA_04G234100
GO:GO:0003674	GO:GO:0003676	GO:GO:0003723	GO:GO:0005488	UniProt:I1JYP9	InterPro:IPR036612
InterPro:KH_dom	InterPro:KH_dom_type_1	InterPro:KH_dom_type_1_sf	EnsemblPlants:KRH64403	ProteinID:KRH64403	ProteinID:KRH64403.1
PFAM:PF00013	PFscan:PS50084	PANTHER:PTHR10288	PANTHER:PTHR10288:SF134	SMART:SM00322	SUPFAM:SSF54791
UniParc:UPI0002339DC2	SEG:seg	:	:	:	:

Description

hypothetical protein

Coordinates

chr4:+:50269820..50275222

Molecular Weight (calculated)

71024.9 Da

IEP (calculated)

5.960

GRAVY (calculated)

-0.462

Length

644 amino acids

Sequence
(BLAST)

001: MERSRSKRNY YYDQDYDSET LARTRPRYNH HYSTGGNHRH RGGGAARHAK TQQDSPLTVT TSYRILCHDL KAGGVIGKSG SIIKSIRQHT GAWINVHELM
101: PGDEERIIEI SDTRRRDPEG RMPSFSPAQE ALLLIHERIL ESDAAFGVAE EDEEYGGRGG GRDRIATRLV VSRMHVGCLL GKGGKIIEQM RMETKTQIRI
201: LPRDHNLPRC VSMSEEIVQV VGNVNAVKNA LVIISSRLRE SQHRDRSHFH GRVHSPERFF SPDDDYVPHV TSGSRRSSVD GASFGSRGSN TNSRNNNHPS
301: LNYAMEPGAA PVVDDAQGFY GEELVFRILC PIEKVDRIIG ESEGIVEFLQ NEVGVDVKVT DPVGGSDEQI IIITSEEGPD DELFPAQEAL LHVQTRIVDL
401: VLDKDNTITT RLVVPSSEIE CLDGKDVSLS EIRRLTGANI QILPRDELPL CVAKTDELVQ IVGEIKAARD AVVEVTSRLR SYLYRDFFQR DTVPLPAPLP
501: GAAASSSNNI VPVTETPTTY QNLQTVAAAL PSKETGGSST ETGKQKESDR RDDLLSGLNR IAVPLVTRST LEVVLPEYAV PKLVAKSKSK LAQISELSGA
601: NVTLVEDRPD VTQKIIQISG TPEQAERAQS LLQGFILSTQ EDGP

Best Arabidopsis Sequence Match ( AT5G53060.1 )
(BLAST)

001: MERSRSKRNY HYDQDYDGDS MPRSKPRYNN NYHFGGGGGG NNRYRGGGGG GGGNGRPSKS HPETMATTTY RILCHDAKAG GVIGKSGTII KSIRQHTGAW
101: INVHELVPGD AERIIEISDN RRRDPDGRMP SFSPAQEALF SVHDRILESE AQFGYGGPPP EEEEDYGGVR PGGGRVVTRL VVSRMHVGCL LGKGGKIIEQ
201: MRIETKTHIR ILPRESNLPR CVSLSEEIVQ IVGELNAVKN ALAIVSSRLR ESQHRDRSNF QGRSHSPERS FAAAGDDYMP QLRRQSSDRF PRGNFRNNNF
301: SSRQSNYAEE APAVPVGENV YSEELVFQIL CPADKIVRVV GESQGIIDLL QNEIGVDVRV SDPVAGSDEQ IITISSEEAP DDPFFPAQEA LLHIQTQIID
401: LIPDKDNLIT TRLLVPSRDS ICLEGKAGSV SEISRLTGTS VQILAREEIP RCASINDVVI QITGEIRAAR EALVELTLLL RSHMFKELSQ KETPPASTST
501: TGPLEGVAGV MEVASSNNTI QSREGPTSSN LNLQQVSTIL PQFKEGFGSV AKAGESEHRE EVPVTTSRMA VPLVTRSTLE VVLPEAVVPK LVTKSRNKLA
601: QISEWSGASV TIVEDRPEET QNIIRISGTP EQAERAQSLL QGFILSIQED GP

Arabidopsis Description

RCF3RNA-binding KH domain-containing protein RCF3 [Source:UniProtKB/Swiss-Prot;Acc:Q8W4B1]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.