CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, nucleus

Predictor Summary:

nucleus 3
cytosol 2
mitochondrion 1
endoplasmic reticulum 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913
mitochondrion: 28499913
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG89015
KRH24906
KRH37677

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G41840.1	KRG89015	AT4G02220.1	21798944
AT2G41840.1	KRH24906	AT4G02220.1	21798944
AT2G41840.1	KRH37677	AT4G02220.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH27864	Soybean	nucleus	99.64	99.64
KRH27874	Soybean	nucleus	99.27	99.27
KRH60418	Soybean	mitochondrion, nucleus	95.64	96.69
KRH41719	Soybean	nucleus	46.91	92.81
Os07t0207400-01	Rice	cytosol, golgi, nucleus, plasma membrane	52.36	87.27
GSMUA_AchrUn_...	Banana	cytosol	68.73	87.1
GSMUA_Achr1P22950_001	Banana	cytosol, endoplasmic reticulum, nucleus	79.64	86.22
VIT_08s0007g08370.t01	Wine grape	cytosol, endoplasmic reticulum, nucleus	85.45	86.08
PGSC0003DMT400063904	Potato	cytosol	87.64	86.07
Solyc04g074300.2.1	Tomato	nucleus	87.64	86.07
GSMUA_Achr1P12890_001	Banana	cytosol	68.36	85.84
Solyc04g063290.2.1	Tomato	nucleus	87.27	85.71
CDY06903	Canola	cytosol	79.27	85.16
GSMUA_Achr2P07800_001	Banana	cytosol	68.0	84.62
PGSC0003DMT400079142	Potato	cytosol	79.27	84.17
Zm00001d034550_P001	Maize	plasma membrane, plastid	83.64	83.94
Os03t0807800-01	Rice	nucleus	83.64	83.94
CDY45881	Canola	cytosol	83.64	83.33
AT3G57490.1	Thale cress	cytosol	83.64	83.33
Zm00001d013034_P001	Maize	plastid	82.91	83.21
AT1G58380.1	Thale cress	cytosol	85.82	83.1
EER93242	Sorghum	cytosol	82.18	82.48
EER93243	Sorghum	cytosol	82.18	82.48
TraesCS5B01G468500.1	Wheat	plastid	81.82	82.12
CDY22542	Canola	cytosol	83.64	81.56
Bra000242.1-P	Field mustard	cytosol	83.27	81.21
TraesCS5A01G458900.1	Wheat	cytosol	81.09	81.09
Zm00001d019147_P001	Maize	plasma membrane, plastid	82.18	81.0
CDX79908	Canola	cytosol	83.64	80.7
TraesCS5D01G470000.1	Wheat	extracellular, golgi, plastid, unclear	81.82	80.65
EER96052	Sorghum	cytosol	81.82	80.65
AT1G58684.1	Thale cress	cytosol	83.27	80.63
AT1G58983.1	Thale cress	cytosol	83.27	80.63
AT1G59359.1	Thale cress	cytosol	83.27	80.63
CDY14790	Canola	cytosol	83.27	80.35
AT2G41840.1	Thale cress	cytosol	83.27	80.35
Bra004654.1-P	Field mustard	cytosol	83.27	80.35
Bra016913.1-P	Field mustard	cytosol	83.27	80.35
CDY44553	Canola	cytosol	81.45	80.0
Bra035439.1-P	Field mustard	cytosol	81.45	80.0
TraesCS3A01G366100.1	Wheat	golgi	80.73	79.86
TraesCS3B01G376600.1	Wheat	nucleus	80.73	79.86
TraesCS3D01G338200.1	Wheat	cytosol	80.36	79.5
HORVU3Hr1G086050.1	Barley	cytosol	79.64	78.78
CDY42063	Canola	mitochondrion	27.64	78.35
CDY35397	Canola	cytosol, extracellular	83.27	75.58
HORVU5Hr1G109910.2	Barley	cytosol	73.82	67.0
GSMUA_Achr8P31470_001	Banana	cytosol	37.45	66.88
OQU76471	Sorghum	cytosol	48.0	60.27
PGSC0003DMT400013052	Potato	cytosol	23.64	60.19
CDY65554	Canola	cytosol	10.91	55.56
Bra027857.1-P	Field mustard	cytosol, mitochondrion, nucleus	10.18	43.75
OQU90562	Sorghum	cytosol, plastid	29.82	42.49

Protein Annotations

EntrezGene:100809096	MapMan:17.1.3.1.2	Gene3D:3.30.160.770	Gene3D:3.30.230.10	EMBL:ACUP02000767	ncoils:Coil
EnsemblPlantsGene:GLYMA_02G085300	GO:GO:0003674	GO:GO:0003676	GO:GO:0003723	GO:GO:0003735	GO:GO:0005198
GO:GO:0005488	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005829
GO:GO:0005840	GO:GO:0006412	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987
GO:GO:0015935	GO:GO:0019538	GO:GO:0022627	GO:GO:0030529	UniProt:I1JDH6	InterPro:IPR013810
InterPro:IPR014721	EnsemblPlants:KRH70352	ProteinID:KRH70352	ProteinID:KRH70352.1	PFAM:PF00333	PFAM:PF03719
ScanProsite:PS00585	PFscan:PS50881	PANTHER:PTHR13718	PANTHER:PTHR13718:SF79	InterPro:Ribosomal_S5	InterPro:Ribosomal_S5_C
InterPro:Ribosomal_S5_D2-typ_fold	InterPro:Ribosomal_S5_D2-typ_fold_subgr	InterPro:Ribosomal_S5_N	InterPro:Ribosomal_S5_N_CS	InterPro:Ribosomal_S5_euk/arc	SUPFAM:SSF54211
SUPFAM:SSF54768	TIGRFAMs:TIGR01020	UniParc:UPI0001BA842D	SEG:seg	:	:

Description

hypothetical protein

Coordinates

chr2:+:7426828..7428512

Molecular Weight (calculated)

30233.9 Da

IEP (calculated)

11.056

GRAVY (calculated)

-0.440

Length

275 amino acids

Sequence
(BLAST)

001: MAERGGGERG GDRGGFGRGF GGRGRGGDRG RGRRRGPRRE EEEKWVPVTK LGRLVKEGRI RSLEQIYLHS LPIKEHQIID TLVGPTLKDE VMKITPVQKQ
101: TRAGQRTRFK AFVVVGDNNG HVGLGVKCSK EVATAIRGAI ILAKLSVIPV RRGYWGNKIG KPHTVPCKVT GKCGSVTVRM VPAPRGSGIV AARVPKKVLQ
201: FAGIDDVFTS SRGSTKTLGN FVKATFDCLM KTYGFLTPEF WKETRFSKSP FQEYTDLLAK PTGKTLILEE ERVDA

Best Arabidopsis Sequence Match ( AT3G57490.1 )
(BLAST)

001: MAERGVERGG DRGDFGRGFG GRGGGRGGPR GRGRRAGRAP EEEKWVPVTK LGRLVKEGKI TKIEQIYLHS LPVKEYQIID LLVGPSLKDE VMKIMPVQKQ
101: TRAGQRTRFK AFIVVGDSNG HVGLGVKCSK EVATAIRGAI ILAKLSVVPI RRGYWGNKIG KPHTVPCKVT GKCGSVTVRM VPAPRGSGIV AARVPKKVLQ
201: FAGIDDVFTS SRGSTKTLGN FVKATFDCLQ KTYGFLTPEF WKETRFSKSP YQEHTDFLLI PPGVKISEVV VDKSVE

Arabidopsis Description

RPS2D40S ribosomal protein S2-4 [Source:UniProtKB/Swiss-Prot;Acc:Q9SCM3]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.