Factsheet for Os06t0728600-00

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

nucleus 2
cytosol 2
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plasma membrane
Any Predictor:	cytosol, mitochondrion, nucleus
BaCelLo:	nucleus
MultiLoc:	cytosol
PProwler:	mitochondrion
WoLF PSORT:	nucleus
YLoc:	cytosol

plasma membrane: 19502382
plasma membrane: 27800704

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 19502382 doi

M Fujiwara, S Hamada, M Hiratsuka, Y Fukao, T Kawasaki, K Shimamoto

Laboratory of Plant Protein Analysis, Plant Education Unit, Nara Institute of Science and Technology, Takayama, Ikoma, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os01t0314000-01
Os01t0314100-01
Os01t0653350-00
Os01t0653800-02
Os02t0514700-00
Os02t0537700-01
Os02t0793100-01
Os04t0394200-01
Os05t0297001-01
Os07t0675000-02
Os08t0243900-01
Os09t0407950-01
Os09t0408000-01
Os11t0175900-01
Os12t0207300-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G20760.1	Os12t0207300-01	AT1G47830.1	24529374
AT1G20760.1	Os02t0793100-01	AT3G01780.1	24529374
AT1G20760.1	Os11t0175900-01	AT3G01780.1	24529374
AT1G20760.1	Os07t0675000-02	AT3G06810.1	24529374
AT1G20760.1	Os02t0537700-01	AT3G11630.1	24529374
AT1G20760.1	Os09t0407950-01	AT3G50590.1	24529374
AT1G20760.1	Os09t0408000-01	AT3G50590.1	24529374
AT1G20760.1	Os01t0653350-00	AT5G24710.1	24529374
AT1G20760.1	Os01t0653800-02	AT5G24710.1	24529374
AT1G20760.1	Os05t0297001-01	AT5G24710.1	24529374
AT1G20760.1	Os01t0314000-01	AT5G55070.1	24529374
AT1G20760.1	Os01t0314100-01	AT5G55070.1	24529374
AT1G20760.1	Os02t0514700-00	AT5G55070.1	24529374
AT1G20760.1	Os04t0394200-01	AT5G55070.1	24529374
AT1G20760.1	Os08t0243900-01	AT5G57460.1	24529374

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH74100	Soybean	cytosol	8.39	56.39
Zm00001d037125_P009	Maize	cytosol	74.94	55.46
Zm00001d014862_P002	Maize	cytosol	73.38	54.17
EER89004	Sorghum	cytosol	74.16	54.08
TraesCS7D01G539600.2	Wheat	cytosol	76.4	52.86
TraesCS7A01G553100.1	Wheat	cytosol	76.51	52.86
TraesCS7B01G476800.3	Wheat	cytosol	76.17	52.42
HORVU7Hr1G121230.4	Barley	cytosol	74.72	51.54
VIT_03s0038g03870.t01	Wine grape	cytosol	42.39	41.65
KRH14013	Soybean	endoplasmic reticulum	44.18	38.09
GSMUA_Achr4P11750_001	Banana	cytosol	44.85	37.37
VIT_18s0122g01080.t01	Wine grape	cytosol	40.16	35.69
Solyc02g082540.2.1	Tomato	nucleus	41.28	35.48
KRH74101	Soybean	endoplasmic reticulum	33.78	35.2
KRH60909	Soybean	endoplasmic reticulum	38.81	32.67
Os03t0130500-01	Rice	plasma membrane	38.93	32.58
CDY01356	Canola	cytosol	35.68	32.55
CDY29508	Canola	cytosol	36.02	32.43
CDY66337	Canola	cytosol	35.79	32.32
CDX96625	Canola	cytosol	35.23	32.14
Bra025842.1-P	Field mustard	cytosol	35.91	32.0
Bra012247.1-P	Field mustard	cytosol	35.57	31.8
CDY31869	Canola	cytosol	35.23	31.79
KRH51589	Soybean	endoplasmic reticulum	38.14	31.69
CDY33644	Canola	cytosol	34.68	31.19
AT1G20760.1	Thale cress	cytosol	35.12	30.81
CDY08192	Canola	cytosol	39.26	30.18
CDY38814	Canola	cytosol	38.37	29.98
CDY00394	Canola	cytosol	38.37	29.98
CDY04654	Canola	cytosol	39.37	29.91
Bra012288.1-P	Field mustard	cytosol	37.02	29.21
CDY01320	Canola	cytosol	38.59	29.21
CDX96582	Canola	cytosol	36.91	28.87
Bra016413.1-P	Field mustard	cytosol	36.69	28.77
Bra017933.1-P	Field mustard	cytosol	39.26	28.47
Solyc04g082560.2.1	Tomato	cytosol	38.93	28.34
AT1G21630.2	Thale cress	cytosol	39.49	28.31
Bra016455.1-P	Field mustard	nucleus, plastid	34.45	27.14
Os06t0687900-01	Rice	cytosol	2.91	21.67
Os02t0158100-01	Rice	cytosol	10.74	17.68
Os06t0687800-01	Rice	plasma membrane	10.63	17.46
Os04t0669300-01	Rice	plasma membrane	9.96	16.42
PGSC0003DMT400025666	Potato	cytosol	0.67	3.7
Solyc02g082530.1.1	Tomato	nucleus	0.0	0.0

Protein Annotations

Gene3D:1.10.238.10	MapMan:22.1.4.7	UniProt:A0A0P0X1C3	ProteinID:BAS99624.1	ncoils:Coil	InterPro:EF-hand-dom_pair
InterPro:EF_Hand_1_Ca_BS	InterPro:EF_hand_dom	InterPro:EH_dom	GO:GO:0003674	GO:GO:0005488	GO:GO:0005509
GO:GO:0005515	InterPro:IPR000261	InterPro:IPR002048	EnsemblPlantsGene:Os06g0728600	EnsemblPlants:Os06t0728600-00	PFAM:PF12763
PFAM:PF13202	ScanProsite:PS00018	PFscan:PS50031	PFscan:PS50222	PANTHER:PTHR11216	PANTHER:PTHR11216:SF116
SMART:SM00027	SMART:SM00054	SUPFAM:SSF47473	UniParc:UPI000393B2C6	SEG:seg	:

Description

EPS15 homology (EH) domain containing protein. (Os06t0728600-00)

Coordinates

chr6:+:31013746..31021183

Molecular Weight (calculated)

96691.9 Da

IEP (calculated)

6.026

GRAVY (calculated)

-0.658

Length

894 amino acids

Sequence
(BLAST)

001: MEAAFDAYFR AADLDRDGRI SGQEAVAFFK ASALPQPVLA QIWTYADKNR TGFLGREDFY NALRLVTVAQ SGRELTPDIV RSALYGPAAA KIPAPRINVS
101: TPLPNATSVT SPLQPTQAPR PAQQSLAIQG SQGPLSTSLN PQVLQPGNVV RPPQASIANT PAQAIAPRAP AGGVPNHTVP ATTGLSTDWF NGKKSASPLG
201: VTSQTPTRGV SPQVNLATAG IPTQSSTPIA GYGSHTPAST TSVKANSADL NLLSSPPAAN DSKALVPLGN GLSSASTFGV DPFAATPQAK QDSSSPPVVS
301: NSLPSANALG PSAGPHHPPK PLQTGPMQGV ASLPSQPAPK QNQFNSMPSA PAPMGSFPGG QIPSNTNQSQ APWPKITQAD VRKYMIVFIK VDRDRDGKIT
401: GEEARNLFLS WRLPRELLRK VWDLSDQDKD GMLSFREFCT AVYLMERHRE QRPLPDVLPD GIWAEGISLP STGQFAENPT GPAPHPSAGF TSRAMPGQHH
501: GMPPSSMKPP PQRPLSLDAD DAVRTEKQKP KIPVLEEHLT GQLSKEERSA LDAKFKEASD ADKKVQELEK EILDSREKTE FYRTKMQELI LYKSRCDNRF
601: NEVSERMSAD KREVQSLAAK YDERCKKVGD VASKLSMDEA TFREIQEKKL EIYNAIVKLQ KGDGNDEKLQ ERANQIQSDL EELVKSLNEQ CKRYGLRAKP
701: TTLVELPFGW QPGIQETAAV WDEEWDKFGD DGFSTIKELT VEMEPPVVQK DQPTVEDSKV STNGPSAPTS TEKEDSRGDK SAAASEQTVE PDATPSDSKT
801: VAAKSPPVSP VKNTKDGHSD ERDKKQSGTN DTSSRAIESV SNNGGADSPV HGEKRDDSHY WGPSFDNGDD NDSLWNFNRK VQLLSCFCAT VSCL

Best Arabidopsis Sequence Match ( AT1G21630.3 )
(BLAST)

0001: MAAPRPTGGQ DLFDTYFRRA DLDGDGHISG AEAVAFFQGS NLPKHVLAQV WSYADSKKAG YLGRAEFYNA LKLVTVAQSR RELTAEIVKA AIYSPASANI
0101: PAPKINLAAT PSPQPRGVLP ATQAQGVTSM PSVAAGVRGP HMGGTVSTSN QQVVPGQQNQ FTGIPPSQTQ QNFQSPGMPA GGTNAPRPAN QPMPSDWLSG
0201: RSVGPSGNVN SQIPSSQSTY GLTAPNSTAN HITKPHITPA VTSSTTTRPQ ESAPVHNPQE SSATFGSRVS NVPSNQLVPK DPKELAASGN GFTSDSLFGD
0301: VFSVTSTQPK QHPTGSASTT GISSVTTGTV AGPEITQSVV RQSSIPQQGS LSQHAVGVQT QLTGNSGQPY TSSGAASGPP GSTVGVGISA TSQLAQRPPH
0401: PHSQPQPRPQ GQSQPPWPKM TPADVQKYTK VFVQVDTDRD GKITGNQARN LFLSWRLPRD ALKQVWDLSD QDNDSMLSLR EFCIAVYLME RYREGRPLPP
0501: VFPSSIIHSE SMFTSPGQSV APHGNASWGH PQGFQQQPHP GGLRPPAGPK GKPPRPVPLS PSDGMVQPTQ PKRKMPVLEK PLVDQLSKEE QDSLNTKFEE
0601: ATAVDKKVDE LEKEIADSKQ KIDFFRAKMQ ELVLYKSRCD NRYNEIAERV LGDKRELESL AKKYEEKYKK SGNVGSKLTI EEATFRDIQE KKMELYQAIV
0701: KFEEGKLDDS IVKERTEHIQ SGLEELIKNL NERCKQYGVR GKPTSLVELP FGWQPGIQEG AADWDEDWDK LEDEGFTFVK ELTLDIQNVI APPKEKSSAW
0801: RKEVDVSSKE GEDVSFSDAD SKTGKKQSSG EEDSEQSEGK TSDVDARDKN GSLDDSKVRK GIEADSSPRT KDTRSENGHD DGESTASAGK TVNYDSHDET
0901: DSVSSVNPDN GKDKDHGKYD SGFGFGFGFD DFSIKPIKTG STISNDFLPP KLSIFADSVP SPPANASDVS PTKPSLFADS VPSTPATNNA SYPGQKSFFD
1001: DSVPSTPAYP GNLFAEKKSY FDDSVPSTPA YSTSDFGGKP FASETPRSDN LFPGRSPFMF DSVPSTPAAH DDFSNNSFSR FDSFNSNNND AFSLSRTDSM
1101: RSTSEPDPFA SRFDSFNYQR YDSFNAQSYD SSSNNNASET PKASLTRFDS IGSTRDSDYS HGFGFDDHDP FGSTGPFKTT TTTAETPRSS DNWNAF

Arabidopsis Description

Calcium-binding EF hand family protein [Source:UniProtKB/TrEMBL;Acc:F4HY32]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.