Factsheet for TraesCS2B01G066400.1

Subcellular Localization

min:

: max

Winner_takes_all: plastid

Predictor Summary:

plastid 3
extracellular 3
endoplasmic reticulum 3
vacuole 4
plasma membrane 4
golgi 3

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plastid
Any Predictor:	plastid, secretory
ChloroP:	plastid
iPSORT:	secretory
MultiLoc:	vacuole
Predotar:	secretory
PProwler:	secretory
TargetP:	plastid
WoLF PSORT:	plastid
YLoc:	plasma membrane

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
TraesCS2D01G052300.1	Wheat	plastid	94.7	94.88
TraesCS2A01G051500.1	Wheat	peroxisome	60.98	94.43
HORVU2Hr1G008270.1	Barley	peroxisome	51.52	87.46
HORVU2Hr1G008300.1	Barley	plastid	86.55	86.88
TraesCS2B01G066900.1	Wheat	nucleus, unclear	79.55	79.7
TraesCS2B01G066800.1	Wheat	vacuole	76.52	76.52
TraesCS5B01G302700.1	Wheat	vacuole	73.86	75.0
TraesCS7B01G273600.1	Wheat	vacuole	64.58	65.96
Os06t0549600-01	Rice	extracellular	58.9	59.13
TraesCS7B01G273700.1	Wheat	vacuole	55.87	56.73
TraesCS4B01G391900.1	Wheat	vacuole	54.17	55.86
TraesCS4B01G358600.1	Wheat	golgi	54.17	54.68
TraesCS7B01G026000.1	Wheat	cytosol	33.71	53.61
TraesCS7B01G273500.1	Wheat	plastid	55.11	53.59
TraesCS2B01G572400.1	Wheat	unclear	53.79	53.58
TraesCS3B01G070000.1	Wheat	mitochondrion	27.84	52.13
TraesCS4B01G104000.1	Wheat	vacuole	52.84	51.29
TraesCS3B01G132200.1	Wheat	extracellular, plasma membrane, vacuole	50.38	50.38
TraesCS2B01G172300.1	Wheat	vacuole	50.0	49.91
TraesCS2B01G145300.1	Wheat	plasma membrane	53.41	49.3
TraesCS1B01G456900.1	Wheat	vacuole	46.21	49.0
TraesCS1B01G469800.1	Wheat	vacuole	49.81	48.88
TraesCS7B01G273900.1	Wheat	vacuole	47.92	48.65
TraesCS7B01G273800.1	Wheat	plasma membrane	47.54	48.18
TraesCS2B01G017300.1	Wheat	plastid	37.31	48.17
TraesCS7B01G388200.1	Wheat	extracellular, plasma membrane, vacuole	47.54	48.08
TraesCS2B01G145500.1	Wheat	vacuole	52.84	47.94
PGSC0003DMT400080465	Potato	cytosol	35.23	45.81
TraesCS2B01G016900.1	Wheat	vacuole	44.89	45.75
GSMUA_Achr2P15490_001	Banana	golgi, vacuole	35.98	45.35
KRH36852	Soybean	plastid	37.5	44.7
GSMUA_Achr4P31040_001	Banana	vacuole	45.08	44.57
PGSC0003DMT400080468	Potato	vacuole	43.56	43.56
PGSC0003DMT400054391	Potato	cytosol, nucleus, peroxisome	35.42	43.39
KRH42268	Soybean	vacuole	43.18	43.1
KRH42267	Soybean	vacuole	42.99	43.07
PGSC0003DMT400080467	Potato	vacuole	43.75	42.94
Solyc06g065060.1.1	Tomato	extracellular	43.75	42.94
VIT_12s0028g01780.t01	Wine grape	plasma membrane, vacuole	43.56	42.91
KRH58391	Soybean	vacuole	42.99	42.83
CDX77574	Canola	cytosol	32.39	42.75
CDY31432	Canola	vacuole	42.8	42.56
CDY09546	Canola	extracellular, vacuole	43.18	42.54
Solyc06g065070.1.1	Tomato	vacuole	43.18	42.54
KRH42808	Soybean	extracellular, vacuole	42.99	42.43
Bra024719.1-P	Field mustard	peroxisome, plastid, vacuole	36.17	42.26
CDY02321	Canola	extracellular, vacuole	42.8	42.16
KRH42273	Soybean	nucleus	42.05	42.13
KRH42274	Soybean	mitochondrion, nucleus	41.67	42.06
CDY66956	Canola	peroxisome, plasma membrane, plastid	33.52	42.04
Bra020915.1-P	Field mustard	vacuole	42.23	42.0
KRH58398	Soybean	plasma membrane, vacuole	42.23	42.0
KRH42266	Soybean	vacuole	42.8	41.93
CDX76449	Canola	vacuole	42.23	41.92
CDY59834	Canola	peroxisome, plasma membrane, plastid	35.61	41.87
CDX94008	Canola	golgi	42.42	41.87
VIT_10s0003g05390.t01	Wine grape	extracellular	42.42	41.87
KRH42269	Soybean	vacuole	42.05	41.81
AT4G20840.1	Thale cress	plasma membrane	42.61	41.74
KRH58387	Soybean	plastid, vacuole	43.37	41.71
PGSC0003DMT400054564	Potato	cytosol, plastid	39.02	41.7
KRH36842	Soybean	vacuole	42.05	41.65
VIT_03s0017g01470.t01	Wine grape	vacuole	24.62	41.53
Bra027549.1-P	Field mustard	peroxisome	35.23	41.52
CDX98675	Canola	golgi	42.05	41.5
VIT_12s0028g01790.t01	Wine grape	plastid	42.8	41.47
AT1G30740.2	Thale cress	vacuole	42.05	41.42
CDX94009	Canola	vacuole	42.23	41.37
KRH11826	Soybean	nucleus	42.61	41.36
CDY29118	Canola	cytosol, peroxisome, plastid	30.49	41.28
Bra014886.1-P	Field mustard	extracellular	41.1	41.25
Bra019831.1-P	Field mustard	plasma membrane, vacuole	41.48	41.24
CDX97984	Canola	plasma membrane, vacuole	41.48	41.24
KRH58389	Soybean	nucleus	41.86	41.23
Bra038798.1-P	Field mustard	vacuole	42.05	41.19
CDX98676	Canola	vacuole	42.05	41.19
PGSC0003DMT400054571	Potato	cytosol	31.63	41.13
CDY49803	Canola	vacuole	41.29	41.13
PGSC0003DMT400054387	Potato	plasma membrane, vacuole	41.29	41.13
Bra038799.1-P	Field mustard	golgi	41.67	41.12
TraesCS4B01G222500.1	Wheat	plasma membrane	43.94	40.99
CDY69093	Canola	vacuole	41.48	40.93
KRH36840	Soybean	nucleus	42.42	40.88
KRH11844	Soybean	vacuole	41.86	40.85
PGSC0003DMT400054385	Potato	plastid	38.26	40.81
Bra027552.1-P	Field mustard	peroxisome	26.33	40.76
KRH41927	Soybean	nucleus	40.91	40.75
Solyc02g070110.1.1	Tomato	extracellular, vacuole	40.91	40.75
CDY29117	Canola	vacuole	41.29	40.75
Solyc02g070130.1.1	Tomato	vacuole	41.1	40.71
CDY50728	Canola	extracellular, vacuole	40.53	40.68
PGSC0003DMT400043091	Potato	extracellular	40.72	40.64
Solyc02g070090.1.1	Tomato	extracellular	41.1	40.64
KRH36853	Soybean	vacuole	41.29	40.6
KRH11845	Soybean	vacuole	41.1	40.56
CDY72595	Canola	extracellular, vacuole	29.17	40.53
PGSC0003DMT400054560	Potato	cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	27.84	40.5
AT1G30730.1	Thale cress	extracellular	40.34	40.49
VIT_10s0003g05550.t01	Wine grape	vacuole	40.72	40.49
VIT_10s0003g05580.t01	Wine grape	plasma membrane, vacuole	40.72	40.49
Solyc10g054480.1.1	Tomato	plastid	23.3	40.46
KRH56395	Soybean	nucleus	40.53	40.45
KRH58399	Soybean	nucleus	40.91	40.45
KRH11827	Soybean	vacuole	41.1	40.41
KRH58388	Soybean	vacuole	41.86	40.4
Bra023122.1-P	Field mustard	vacuole	40.53	40.38
AT1G26380.1	Thale cress	endoplasmic reticulum	40.91	40.37
AT5G44410.1	Thale cress	golgi	40.91	40.37
KRH36766	Soybean	vacuole	40.72	40.34
AT1G01980.1	Thale cress	plasma membrane, vacuole	41.29	40.3
CDY68515	Canola	extracellular, plasma membrane	28.98	40.26
Bra013476.1-P	Field mustard	cytosol, plastid	41.1	40.26
KRH56394	Soybean	vacuole	40.15	40.23
PGSC0003DMT400054386	Potato	vacuole	40.53	40.23
CDX67534	Canola	plasma membrane, vacuole	40.91	40.22
Bra033740.1-P	Field mustard	golgi	41.67	40.22
Solyc02g070170.1.1	Tomato	extracellular, vacuole	40.72	40.19
AT5G44360.2	Thale cress	golgi	41.67	40.15
VIT_10s0003g05620.t01	Wine grape	vacuole	40.34	40.11
AT1G11770.1	Thale cress	vacuole	40.72	40.11
CDX94788	Canola	vacuole	40.72	40.11
PGSC0003DMT400046308	Potato	extracellular	41.29	40.07
AT5G44400.1	Thale cress	golgi, vacuole	40.72	40.04
Bra028530.1-P	Field mustard	plasma membrane, vacuole	40.72	40.04
AT1G26390.1	Thale cress	vacuole	40.15	40.0
VIT_12s0028g01770.t01	Wine grape	plasma membrane, vacuole	43.18	40.0
Bra027555.1-P	Field mustard	extracellular, vacuole	40.34	39.96
CDY13104	Canola	extracellular, vacuole	40.34	39.96
KRH36843	Soybean	vacuole	40.34	39.96
CDY71012	Canola	extracellular, vacuole	39.96	39.96
KRH62528	Soybean	endoplasmic reticulum, golgi, vacuole	39.96	39.96
CDY70228	Canola	cytosol, golgi, peroxisome	23.3	39.94
VIT_10s0003g04730.t01	Wine grape	plasma membrane, vacuole	40.34	39.81
AT4G20860.1	Thale cress	vacuole	39.96	39.81
KRH58344	Soybean	vacuole	39.96	39.81
CDY16155	Canola	endoplasmic reticulum, extracellular, vacuole	39.2	39.81
VIT_10s0003g05420.t01	Wine grape	vacuole	40.53	39.7
CDY43653	Canola	golgi	22.92	39.67
Bra033743.1-P	Field mustard	vacuole	40.34	39.66
CDY49011	Canola	vacuole	40.34	39.66
CDY09547	Canola	extracellular, vacuole	39.58	39.66
KRH11825	Soybean	vacuole	39.2	39.66
KRH11830	Soybean	vacuole	36.93	39.63
KRH11824	Soybean	nucleus	40.72	39.59
KRH36839	Soybean	nucleus	40.72	39.59
Solyc02g022850.1.1	Tomato	vacuole	40.53	39.56
Bra023131.1-P	Field mustard	endoplasmic reticulum, plasma membrane, vacuole	39.96	39.51
CDX77568	Canola	endoplasmic reticulum, plasma membrane	39.96	39.51
CDY02326	Canola	endoplasmic reticulum, plasma membrane, vacuole	39.96	39.51
CDY13353	Canola	plasma membrane	40.15	39.48
AT4G20820.1	Thale cress	plastid	39.77	39.47
VIT_10s0003g05470.t01	Wine grape	vacuole	42.23	39.47
AT1G30700.1	Thale cress	vacuole	39.39	39.47
CDY29116	Canola	extracellular, vacuole	39.77	39.4
Bra023126.1-P	Field mustard	extracellular, vacuole	39.39	39.39
Bra014890.1-P	Field mustard	plasma membrane	39.77	39.33
CDX82782	Canola	vacuole	42.42	39.3
CDY31642	Canola	plasma membrane	37.88	39.29
CDX94785	Canola	vacuole	39.96	39.29
AT1G30720.1	Thale cress	vacuole	39.2	39.28
CDX79008	Canola	vacuole	42.23	39.26
AT4G20800.1	Thale cress	endoplasmic reticulum, extracellular, vacuole	39.2	39.2
Bra016272.1-P	Field mustard	peroxisome, plasma membrane, plastid	25.0	39.17
Bra013473.1-P	Field mustard	vacuole	39.02	39.16
CDX79005	Canola	vacuole	39.02	39.16
Solyc09g072660.2.1	Tomato	extracellular	40.72	39.16
KRH49946	Soybean	extracellular, vacuole	31.44	39.15
AT5G44390.1	Thale cress	vacuole	40.15	39.11
VIT_03s0017g01490.t01	Wine grape	vacuole	40.34	39.08
PGSC0003DMT400062414	Potato	plasma membrane, vacuole	40.91	38.99
Solyc02g070070.1.1	Tomato	extracellular	39.2	38.98
Solyc02g070060.1.1	Tomato	vacuole	38.83	38.97
CDY67309	Canola	endoplasmic reticulum, plasma membrane	39.39	38.95
Bra005392.1-P	Field mustard	plasma membrane	39.2	38.91
AT1G26400.1	Thale cress	golgi, vacuole	38.83	38.9
Bra013475.1-P	Field mustard	golgi, vacuole	39.39	38.88
CDX79007	Canola	golgi, vacuole	39.39	38.88
CDY29122	Canola	cytosol	14.2	38.86
CDX85019	Canola	extracellular	38.07	38.8
PGSC0003DMT400086243	Potato	vacuole	38.64	38.78
CDX82776	Canola	endoplasmic reticulum, vacuole	39.2	38.76
PGSC0003DMT400054393	Potato	plasma membrane, vacuole	39.2	38.76
Solyc02g070200.1.1	Tomato	vacuole	39.58	38.63
Bra006964.1-P	Field mustard	endoplasmic reticulum, extracellular, vacuole	39.2	38.62
VIT_10s0003g05570.t01	Wine grape	plasma membrane, vacuole	40.72	38.6
PGSC0003DMT400054574	Potato	mitochondrion, peroxisome	37.5	38.6
CDX79034	Canola	plastid	38.07	38.58
PGSC0003DMT400089776	Potato	golgi, vacuole	39.96	38.57
Solyc02g069690.1.1	Tomato	golgi, vacuole	39.96	38.57
AT1G26420.1	Thale cress	vacuole	38.64	38.56
Bra027547.1-P	Field mustard	vacuole	39.39	38.52
Solyc02g070180.1.1	Tomato	plasma membrane, vacuole	39.39	38.52
CDX84280	Canola	plasma membrane	37.12	38.51
CDY34627	Canola	vacuole	39.02	38.5
TraesCS3B01G406900.1	Wheat	vacuole	38.83	38.46
AT4G20830.1	Thale cress	vacuole	41.48	38.42
AT2G34790.1	Thale cress	golgi, vacuole	38.64	38.35
Bra013501.1-P	Field mustard	vacuole	38.64	38.35
CDY13105	Canola	vacuole	39.2	38.33
Bra024717.1-P	Field mustard	vacuole	38.26	38.33
CDY16156	Canola	vacuole	38.26	38.33
Bra013478.1-P	Field mustard	endoplasmic reticulum, vacuole	37.88	38.31
Bra031780.1-P	Field mustard	vacuole	39.02	38.29
CDY34829	Canola	vacuole	39.02	38.29
PGSC0003DMT400057528	Potato	extracellular	39.02	38.29
Solyc02g079440.1.1	Tomato	nucleus	39.02	38.29
CDX82775	Canola	extracellular, vacuole	38.26	38.26
VIT_03s0017g01420.t01	Wine grape	cytosol, peroxisome, vacuole	39.96	38.22
CDX79010	Canola	cytosol	19.32	38.2
CDX82785	Canola	endoplasmic reticulum, extracellular, vacuole	37.69	38.12
AT1G30760.2	Thale cress	golgi	39.77	38.04
AT1G30710.1	Thale cress	extracellular, plasma membrane, vacuole	38.26	38.04
KRH42272	Soybean	vacuole	38.83	38.03
KRH42271	Soybean	endoplasmic reticulum	41.48	37.95
PGSC0003DMT400012979	Potato	cytosol	28.6	37.94
CDX82800	Canola	extracellular	37.5	37.93
PGSC0003DMT400036039	Potato	extracellular, plasma membrane, vacuole	39.77	37.91
VIT_10s0003g05450.t01	Wine grape	extracellular	41.1	37.8
CDX79004	Canola	extracellular, vacuole	37.69	37.76
CDY49007	Canola	vacuole	38.64	37.71
KRH41928	Soybean	vacuole	37.88	37.66
Bra013472.1-P	Field mustard	extracellular, vacuole	37.5	37.57
KRH11841	Soybean	vacuole	39.77	37.57
AT1G34575.1	Thale cress	vacuole	37.31	37.38
CDX82774	Canola	extracellular, vacuole	37.31	37.38
CDY69774	Canola	endoplasmic reticulum, extracellular, plastid, vacuole	36.93	37.36
KRH58395	Soybean	endoplasmic reticulum	41.1	37.29
Solyc02g079450.1.1	Tomato	extracellular	37.88	37.24
Solyc02g079460.1.1	Tomato	extracellular, golgi, vacuole	37.5	37.22
CDX94780	Canola	plasma membrane, vacuole	38.26	37.2
KRH36851	Soybean	vacuole	39.58	36.99
PGSC0003DMT400057680	Potato	extracellular, plasma membrane, vacuole	37.5	36.87
AT1G26410.1	Thale cress	golgi	38.45	36.78
CDX94786	Canola	vacuole	36.93	36.72
Bra022992.1-P	Field mustard	mitochondrion	33.14	36.69
CDX79582	Canola	mitochondrion	33.14	36.69
CDY70640	Canola	mitochondrion	33.14	36.69
Bra021930.1-P	Field mustard	plasma membrane	34.85	36.65
KRH42276	Soybean	nucleus	41.1	36.59
Bra014885.1-P	Field mustard	plasma membrane, vacuole	36.74	36.53
CDX77576	Canola	plasma membrane, vacuole	36.55	36.35
CDY50727	Canola	plasma membrane, vacuole	36.55	36.35
AT5G44380.2	Thale cress	mitochondrion, plastid	39.96	36.32
KRH71761	Soybean	mitochondrion, plastid, vacuole	29.17	36.24
Solyc02g079470.1.1	Tomato	endoplasmic reticulum, vacuole	36.93	36.18
AT5G44440.2	Thale cress	golgi	40.91	35.82
CDY68914	Canola	golgi, plastid	29.36	35.8
KRH36841	Soybean	vacuole	26.52	35.62
CDY70679	Canola	cytosol, mitochondrion, peroxisome	15.72	35.62
KRH12123	Soybean	plastid	30.87	35.59
Bra027553.1-P	Field mustard	vacuole	29.17	35.4
Bra023123.1-P	Field mustard	plasma membrane, vacuole	34.28	35.35
CDY02318	Canola	plasma membrane, vacuole	34.28	35.35
CDX82780	Canola	cytosol	14.2	35.05
AT2G34810.1	Thale cress	vacuole	35.8	35.0
Bra005390.1-P	Field mustard	vacuole	35.42	34.69
CDY69038	Canola	vacuole	35.42	34.69
CDY52527	Canola	vacuole	35.42	34.5
TraesCS1B01G448900.1	Wheat	endoplasmic reticulum, extracellular, vacuole	36.55	34.1
Solyc02g079480.1.1	Tomato	plasma membrane, vacuole	36.17	34.05
PGSC0003DMT400057523	Potato	extracellular, golgi, vacuole	35.98	33.75
Bra033739.1-P	Field mustard	plasma membrane, vacuole	37.31	32.4
CDX85018	Canola	cytosol	39.39	31.04
KRG99206	Soybean	extracellular, peroxisome	23.48	30.69

Protein Annotations

Gene3D:3.30.43.10	Gene3D:3.30.465.50	Gene3D:3.40.462.20	MapMan:50.1.1	InterPro:BBE	InterPro:FAD-bd_2
InterPro:FAD-bd_2-like_sf	InterPro:FAD-bd_2_sub1	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0005488
GO:GO:0008150	GO:GO:0008152	GO:GO:0016491	GO:GO:0016614	GO:GO:0050660	GO:GO:0055114
InterPro:IPR016166	InterPro:IPR016167	InterPro:Oxid_FAD_bind_N	PFAM:PF01565	PFAM:PF08031	PFscan:PS51387
PANTHER:PTHR32448	PANTHER:PTHR32448:SF32	SUPFAM:SSF56176	SignalP:SignalP-noTM	EnsemblPlantsGene:TraesCS2B01G066400	EnsemblPlants:TraesCS2B01G066400.1
SEG:seg	:	:	:	:	:

Description

No Description!

Coordinates

chr2B:+:33535078..33536664

Molecular Weight (calculated)

57834.0 Da

IEP (calculated)

10.242

GRAVY (calculated)

-0.064

Length

528 amino acids

Sequence
(BLAST)

001: MARVALVLTI CFLGFSPSLA WSSKSNHTDF LSCFSTKIPS QLVLTPSSRS FKPLLVSSIR NARLVAPATA SPPLCIVTPT QASHVQAVVR CGRSHRVRVR
101: VRSGGHDNEG LSYRSATPNG EAFAVIDLSK FHGVRVDARA ATAWVDSGTT LGELYYRVAT GAPGLGFPAG VCPTVGVGGL ISGGGMGLMM RKNGLSSDNV
201: LDATMIDAEG NLLADKKAMG DDLFWAIRGG GGGNFGIVLS WKLRLVPVPP KVAFFNVTKT MDQGAVDAVT KWQTIAPALP EDLSVRVVVQ KRQATFQSLY
301: LGNCSAVVAT MRSRFPELGL TRRKCKEMSW LQHKAYLYFG DASNNTPLEA LLLNRSMTIG PFVKNKSDYV KKALTRDAWE KIFLWPDGGA VGQLILEPHG
401: GMMNRIADDY TPFPYRSSVL YNIQYVEFWN GTRAHGTPKW LSGLYDFMAP LVSKSPRGAY VNYRDLDIGV NKVVGGVTSY ETAKVWGERY FGLTNFKRLA
501: KIKRKVDATD YFRNEQSVPP LLLIRERA

Best Arabidopsis Sequence Match ( AT1G11770.1 )
(BLAST)

001: MKIFCLILFL ISSFISTSLA VEPPPETIYQ NFLQCFTNQT KAPPNSLADV VLPKTAAAFT PVLRAYIRNA RFNTTATPKP AIVIAARSES HVQAAVICTK
101: SLNIQLKTRS GGHDYEGVSY ISHVPFFVLD MSNLRNITVD PATESAWVGA GATLGEVYYR IWEKTKSHGF PAGVCPTVGA GGHISGGGYG NMIRKYGLSV
201: DYVTDAKIVD VNGQVLDRKG MGEDMFWAIN GGGGASFGVI LAFKIKLVPV PPTVTVFRVE KNLVENATEM VHKWQFVAPK TDPGLFMRLL LQPVTRNKMQ
301: TVRASVVALF LGDQNTVMSM LTKEFPELGL KKENCTEMTW IQSVMWWANN DNATQIKPEI LLDRNPDMAT FGKRKSDFVE KEITKDGLDF LFKKMIEVGK
401: IGLVFNPYGG IMSTVATTKT PFPHRKKLYK IQHSMNWKDP GTEAETSFLQ KAKSFYSYMA PFVTKNPRHT YINYRDLDIG VNTPGPNSYR VAEVFGRMYF
501: GENFDRLVKV KTAVDPQNFF RDEQSIPTLP GKPARR

Arabidopsis Description

Berberine bridge enzyme-like 2 [Source:UniProtKB/Swiss-Prot;Acc:Q9SA99]
SUBAcon: [vacuole]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.