Factsheet for TraesCS4B01G391900.1

Subcellular Localization

min:

: max

Winner_takes_all: vacuole

Predictor Summary:

plastid 1
extracellular 4
endoplasmic reticulum 4
vacuole 6
plasma membrane 5
golgi 4

Predictors

GFP

MS/MS

Papers

Winner Takes All:	vacuole
Any Predictor:	plastid, secretory
ChloroP:	plastid
iPSORT:	secretory
MultiLoc:	vacuole
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	vacuole
YLoc:	plasma membrane

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
TraesCSU01G034600.1	Wheat	vacuole	93.16	92.62
TraesCS5A01G555000.1	Wheat	plasma membrane, vacuole	93.16	92.44
TraesCS4B01G358600.1	Wheat	golgi	83.01	81.26
TraesCS2B01G572400.1	Wheat	unclear	81.05	78.3
TraesCS7B01G273700.1	Wheat	vacuole	74.41	73.27
TraesCS4B01G104000.1	Wheat	vacuole	72.07	67.83
Os06t0549300-00	Rice	extracellular	66.8	65.39
Zm00001d037008_P001	Maize	golgi, vacuole	64.84	64.97
EER89908	Sorghum	plasma membrane, vacuole	67.19	63.82
TraesCS7B01G273600.1	Wheat	vacuole	62.7	62.09
TraesCS5B01G302700.1	Wheat	vacuole	57.42	56.54
TraesCS7B01G273500.1	Wheat	plastid	59.38	55.99
TraesCS3B01G132200.1	Wheat	extracellular, plasma membrane, vacuole	57.23	55.49
TraesCS2B01G066900.1	Wheat	nucleus, unclear	56.84	55.22
TraesCS2B01G066800.1	Wheat	vacuole	56.64	54.92
TraesCS2B01G066400.1	Wheat	plastid	55.86	54.17
TraesCS7B01G026000.1	Wheat	cytosol	34.57	53.31
TraesCS2B01G145300.1	Wheat	plasma membrane	58.4	52.27
TraesCS2B01G017300.1	Wheat	plastid	41.21	51.59
TraesCS7B01G273900.1	Wheat	vacuole	51.56	50.77
TraesCS7B01G273800.1	Wheat	plasma membrane	51.37	50.48
TraesCS7B01G388200.1	Wheat	extracellular, plasma membrane, vacuole	51.37	50.38
TraesCS2B01G145500.1	Wheat	vacuole	55.47	48.8
TraesCS2B01G172300.1	Wheat	vacuole	50.39	48.77
TraesCS2B01G016900.1	Wheat	vacuole	48.24	47.68
TraesCS1B01G456900.1	Wheat	vacuole	46.29	47.59
TraesCS1B01G469800.1	Wheat	vacuole	50.0	47.58
TraesCS3B01G070000.1	Wheat	mitochondrion	25.39	46.1
PGSC0003DMT400080465	Potato	cytosol	36.33	45.81
VIT_03s0017g01470.t01	Wine grape	vacuole	27.93	45.69
GSMUA_Achr2P15490_001	Banana	golgi, vacuole	37.11	45.35
CDY66956	Canola	peroxisome, plasma membrane, plastid	36.72	44.66
CDY59834	Canola	peroxisome, plasma membrane, plastid	39.06	44.54
KRH42274	Soybean	mitochondrion, nucleus	45.31	44.36
KRH36852	Soybean	plastid	38.28	44.24
PGSC0003DMT400080468	Potato	vacuole	45.51	44.13
KRH42273	Soybean	nucleus	44.92	43.64
CDY29118	Canola	cytosol, peroxisome, plastid	33.2	43.59
KRH58398	Soybean	plasma membrane, vacuole	45.12	43.5
Bra024719.1-P	Field mustard	peroxisome, plastid, vacuole	38.28	43.36
Solyc06g065070.1.1	Tomato	vacuole	45.31	43.28
VIT_10s0003g05390.t01	Wine grape	extracellular	45.12	43.18
KRH42808	Soybean	extracellular, vacuole	44.92	42.99
Solyc10g054480.1.1	Tomato	plastid	25.39	42.76
GSMUA_Achr4P31040_001	Banana	vacuole	44.53	42.7
KRH58399	Soybean	nucleus	44.53	42.7
Solyc06g065060.1.1	Tomato	extracellular	44.73	42.57
VIT_10s0003g05550.t01	Wine grape	vacuole	44.14	42.56
PGSC0003DMT400080467	Potato	vacuole	44.53	42.38
VIT_10s0003g05620.t01	Wine grape	vacuole	43.75	42.18
KRH11824	Soybean	nucleus	44.73	42.17
CDY69093	Canola	vacuole	43.95	42.06
PGSC0003DMT400089776	Potato	golgi, vacuole	44.92	42.05
VIT_10s0003g05580.t01	Wine grape	plasma membrane, vacuole	43.55	42.0
CDX98676	Canola	vacuole	44.14	41.93
CDY29117	Canola	vacuole	43.75	41.87
Solyc02g069690.1.1	Tomato	golgi, vacuole	44.73	41.86
Bra016272.1-P	Field mustard	peroxisome, plasma membrane, plastid	27.54	41.84
KRH42269	Soybean	vacuole	43.36	41.81
KRH36839	Soybean	nucleus	44.34	41.8
CDY09546	Canola	extracellular, vacuole	43.75	41.79
Bra038798.1-P	Field mustard	vacuole	43.95	41.74
CDY31432	Canola	vacuole	43.16	41.62
CDY68515	Canola	extracellular, plasma membrane	30.86	41.58
CDY72595	Canola	extracellular, vacuole	30.86	41.58
CDX94009	Canola	vacuole	43.75	41.56
VIT_10s0003g05420.t01	Wine grape	vacuole	43.75	41.56
PGSC0003DMT400054391	Potato	cytosol, nucleus, peroxisome	34.96	41.53
CDX77574	Canola	cytosol	32.42	41.5
VIT_10s0003g04730.t01	Wine grape	plasma membrane, vacuole	43.36	41.5
Bra020915.1-P	Field mustard	vacuole	42.97	41.43
CDY02321	Canola	extracellular, vacuole	43.36	41.42
KRH56394	Soybean	vacuole	42.58	41.37
CDX76449	Canola	vacuole	42.97	41.35
TraesCS4B01G222500.1	Wheat	plasma membrane	45.7	41.34
AT5G44400.1	Thale cress	golgi, vacuole	43.36	41.34
Bra027549.1-P	Field mustard	peroxisome	36.13	41.29
KRH58389	Soybean	nucleus	43.16	41.23
KRH42266	Soybean	vacuole	43.36	41.19
KRH58387	Soybean	plastid, vacuole	44.14	41.17
KRH58388	Soybean	vacuole	43.95	41.13
KRH36766	Soybean	vacuole	42.77	41.09
KRH36843	Soybean	vacuole	42.77	41.09
KRH11844	Soybean	vacuole	43.36	41.04
PGSC0003DMT400043091	Potato	extracellular	42.38	41.02
AT4G20820.1	Thale cress	plastid	42.58	40.98
VIT_12s0028g01780.t01	Wine grape	plasma membrane, vacuole	42.77	40.86
AT4G20840.1	Thale cress	plasma membrane	42.97	40.82
KRH36853	Soybean	vacuole	42.77	40.78
AT5G44410.1	Thale cress	golgi	42.58	40.75
Bra013473.1-P	Field mustard	vacuole	41.8	40.68
Bra006964.1-P	Field mustard	endoplasmic reticulum, extracellular, vacuole	42.58	40.67
Bra023131.1-P	Field mustard	endoplasmic reticulum, plasma membrane, vacuole	42.38	40.64
CDY02326	Canola	endoplasmic reticulum, plasma membrane, vacuole	42.38	40.64
Bra013476.1-P	Field mustard	cytosol, plastid	42.77	40.63
KRH62528	Soybean	endoplasmic reticulum, golgi, vacuole	41.8	40.53
Bra014886.1-P	Field mustard	extracellular	41.6	40.49
CDX79005	Canola	vacuole	41.6	40.49
Bra027552.1-P	Field mustard	peroxisome	26.95	40.47
KRH42268	Soybean	vacuole	41.8	40.45
KRH42272	Soybean	vacuole	42.58	40.45
KRH11827	Soybean	vacuole	42.38	40.41
Bra033740.1-P	Field mustard	golgi	43.16	40.4
VIT_10s0003g05570.t01	Wine grape	plasma membrane, vacuole	43.95	40.4
Bra023122.1-P	Field mustard	vacuole	41.8	40.38
VIT_12s0028g01790.t01	Wine grape	plastid	42.97	40.37
KRH36842	Soybean	vacuole	41.99	40.34
AT5G44360.2	Thale cress	golgi	43.16	40.33
KRH36840	Soybean	nucleus	43.16	40.33
CDY43653	Canola	golgi	24.02	40.33
Bra019831.1-P	Field mustard	plasma membrane, vacuole	41.8	40.3
CDX97984	Canola	plasma membrane, vacuole	41.8	40.3
AT1G30740.2	Thale cress	vacuole	42.19	40.3
CDY70228	Canola	cytosol, golgi, peroxisome	24.22	40.26
PGSC0003DMT400046308	Potato	extracellular	42.77	40.26
AT4G20860.1	Thale cress	vacuole	41.6	40.19
CDY49803	Canola	vacuole	41.6	40.19
KRH58344	Soybean	vacuole	41.6	40.19
KRH58391	Soybean	vacuole	41.6	40.19
Bra013475.1-P	Field mustard	golgi, vacuole	41.99	40.19
CDX79007	Canola	golgi, vacuole	41.99	40.19
CDX94008	Canola	golgi	41.99	40.19
KRH11845	Soybean	vacuole	41.99	40.19
VIT_03s0017g01490.t01	Wine grape	vacuole	42.77	40.18
CDX79034	Canola	plastid	40.82	40.12
CDY50728	Canola	extracellular, vacuole	41.21	40.11
Solyc02g022850.1.1	Tomato	vacuole	42.38	40.11
CDX67534	Canola	plasma membrane, vacuole	41.99	40.04
VIT_03s0017g01420.t01	Wine grape	cytosol, peroxisome, vacuole	43.16	40.04
Bra038799.1-P	Field mustard	golgi	41.8	40.0
VIT_10s0003g05470.t01	Wine grape	vacuole	44.14	40.0
Bra027555.1-P	Field mustard	extracellular, vacuole	41.6	39.96
KRH11826	Soybean	nucleus	42.38	39.89
CDX82776	Canola	endoplasmic reticulum, vacuole	41.6	39.89
Bra028530.1-P	Field mustard	plasma membrane, vacuole	41.8	39.85
CDX94785	Canola	vacuole	41.8	39.85
Bra022992.1-P	Field mustard	mitochondrion	37.11	39.83
CDX79582	Canola	mitochondrion	37.11	39.83
CDY70640	Canola	mitochondrion	37.11	39.83
CDY16155	Canola	endoplasmic reticulum, extracellular, vacuole	40.43	39.81
CDX94788	Canola	vacuole	41.6	39.74
Solyc09g072660.2.1	Tomato	extracellular	42.58	39.71
AT1G26420.1	Thale cress	vacuole	41.02	39.7
KRH56395	Soybean	nucleus	41.02	39.7
Bra033743.1-P	Field mustard	vacuole	41.6	39.66
Bra013501.1-P	Field mustard	vacuole	41.21	39.66
AT1G30700.1	Thale cress	vacuole	40.82	39.66
AT1G34575.1	Thale cress	vacuole	40.82	39.66
KRH42267	Soybean	vacuole	40.82	39.66
CDX82800	Canola	extracellular	40.43	39.66
PGSC0003DMT400054571	Potato	cytosol	31.45	39.66
CDX98675	Canola	golgi	41.41	39.63
CDY13104	Canola	extracellular, vacuole	41.21	39.59
CDX82775	Canola	extracellular, vacuole	40.82	39.58
CDY13353	Canola	plasma membrane	41.41	39.48
CDY49011	Canola	vacuole	41.41	39.48
Bra005392.1-P	Field mustard	plasma membrane	41.02	39.47
AT1G26390.1	Thale cress	vacuole	40.82	39.43
PGSC0003DMT400054387	Potato	plasma membrane, vacuole	40.82	39.43
CDY29116	Canola	extracellular, vacuole	41.02	39.4
AT1G11770.1	Thale cress	vacuole	41.21	39.37
KRH41928	Soybean	vacuole	40.82	39.36
PGSC0003DMT400062414	Potato	plasma membrane, vacuole	42.58	39.35
CDX77568	Canola	endoplasmic reticulum, plasma membrane	41.02	39.33
AT5G44390.1	Thale cress	vacuole	41.6	39.3
CDX84280	Canola	plasma membrane	39.06	39.29
CDY31642	Canola	plasma membrane	39.06	39.29
AT2G34790.1	Thale cress	golgi, vacuole	40.82	39.29
AT1G26400.1	Thale cress	golgi, vacuole	40.43	39.28
Solyc02g070110.1.1	Tomato	extracellular, vacuole	40.62	39.25
AT1G01980.1	Thale cress	plasma membrane, vacuole	41.41	39.19
AT1G30710.1	Thale cress	extracellular, plasma membrane, vacuole	40.62	39.17
Solyc02g070060.1.1	Tomato	vacuole	40.23	39.16
Solyc02g070090.1.1	Tomato	extracellular	40.82	39.14
AT1G30720.1	Thale cress	vacuole	40.23	39.09
Bra013472.1-P	Field mustard	extracellular, vacuole	40.23	39.09
CDX79004	Canola	extracellular, vacuole	40.23	39.09
Bra013478.1-P	Field mustard	endoplasmic reticulum, vacuole	39.84	39.08
Bra027547.1-P	Field mustard	vacuole	41.21	39.07
KRH41927	Soybean	nucleus	40.43	39.06
AT4G20800.1	Thale cress	endoplasmic reticulum, extracellular, vacuole	40.23	39.02
KRH11841	Soybean	vacuole	42.58	39.0
Bra014890.1-P	Field mustard	plasma membrane	40.62	38.95
CDY67309	Canola	endoplasmic reticulum, plasma membrane	40.62	38.95
CDX82785	Canola	endoplasmic reticulum, extracellular, vacuole	39.65	38.89
AT1G26380.1	Thale cress	endoplasmic reticulum	40.62	38.88
CDX85019	Canola	extracellular	39.26	38.8
PGSC0003DMT400054385	Potato	plastid	37.5	38.79
AT4G20830.1	Thale cress	vacuole	43.16	38.77
CDX82782	Canola	vacuole	43.16	38.77
CDX79008	Canola	vacuole	42.97	38.73
CDX82774	Canola	extracellular, vacuole	39.84	38.71
CDY13105	Canola	vacuole	40.82	38.7
PGSC0003DMT400054564	Potato	cytosol, plastid	37.3	38.66
Solyc02g070070.1.1	Tomato	extracellular	40.04	38.61
CDY68914	Canola	golgi, plastid	32.62	38.57
Bra024717.1-P	Field mustard	vacuole	39.65	38.52
CDY16156	Canola	vacuole	39.65	38.52
VIT_10s0003g05450.t01	Wine grape	extracellular	43.16	38.5
KRH11830	Soybean	vacuole	36.91	38.41
AT1G30760.2	Thale cress	golgi	41.41	38.41
PGSC0003DMT400054393	Potato	plasma membrane, vacuole	40.04	38.39
CDY09547	Canola	extracellular, vacuole	39.45	38.33
CDY69774	Canola	endoplasmic reticulum, extracellular, plastid, vacuole	39.06	38.31
CDY71012	Canola	extracellular, vacuole	39.45	38.26
PGSC0003DMT400086243	Potato	vacuole	39.26	38.21
KRH42276	Soybean	nucleus	44.14	38.11
KRH42271	Soybean	endoplasmic reticulum	42.77	37.95
KRH36841	Soybean	vacuole	29.1	37.91
KRH36851	Soybean	vacuole	41.8	37.88
Bra021930.1-P	Field mustard	plasma membrane	37.11	37.85
VIT_12s0028g01770.t01	Wine grape	plasma membrane, vacuole	41.99	37.72
Solyc02g070130.1.1	Tomato	vacuole	39.26	37.71
CDY49007	Canola	vacuole	39.84	37.71
Bra027553.1-P	Field mustard	vacuole	32.03	37.7
AT1G26410.1	Thale cress	golgi	40.62	37.68
Bra014885.1-P	Field mustard	plasma membrane, vacuole	39.06	37.66
PGSC0003DMT400054574	Potato	mitochondrion, peroxisome	37.7	37.62
CDY34627	Canola	vacuole	39.26	37.57
TraesCS3B01G406900.1	Wheat	vacuole	39.06	37.52
Bra023126.1-P	Field mustard	extracellular, vacuole	38.67	37.5
CDX77576	Canola	plasma membrane, vacuole	38.87	37.48
CDY50727	Canola	plasma membrane, vacuole	38.87	37.48
KRH58395	Soybean	endoplasmic reticulum	42.58	37.46
AT1G30730.1	Thale cress	extracellular	38.48	37.45
PGSC0003DMT400054386	Potato	vacuole	38.87	37.41
CDX94780	Canola	plasma membrane, vacuole	39.65	37.38
Bra031780.1-P	Field mustard	vacuole	39.26	37.36
CDY34829	Canola	vacuole	39.26	37.36
KRH11825	Soybean	vacuole	38.09	37.36
CDY29122	Canola	cytosol	14.06	37.31
Solyc02g070170.1.1	Tomato	extracellular, vacuole	38.87	37.2
CDX82780	Canola	cytosol	15.43	36.92
PGSC0003DMT400054560	Potato	cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	26.17	36.91
Bra023123.1-P	Field mustard	plasma membrane, vacuole	36.72	36.72
CDY02318	Canola	plasma membrane, vacuole	36.72	36.72
KRH49946	Soybean	extracellular, vacuole	30.27	36.56
Bra005390.1-P	Field mustard	vacuole	38.48	36.55
CDY69038	Canola	vacuole	38.48	36.55
CDY52527	Canola	vacuole	38.67	36.53
AT5G44440.2	Thale cress	golgi	42.97	36.48
Solyc02g079460.1.1	Tomato	extracellular, golgi, vacuole	37.89	36.47
Solyc02g070200.1.1	Tomato	vacuole	38.48	36.41
KRH12123	Soybean	plastid	32.42	36.24
Solyc02g070180.1.1	Tomato	plasma membrane, vacuole	38.09	36.11
CDX94786	Canola	vacuole	37.3	35.97
PGSC0003DMT400012979	Potato	cytosol	27.93	35.93
Solyc02g079470.1.1	Tomato	endoplasmic reticulum, vacuole	37.7	35.81
PGSC0003DMT400057528	Potato	extracellular	37.5	35.69
CDY70679	Canola	cytosol, mitochondrion, peroxisome	16.21	35.62
CDX79010	Canola	cytosol	18.55	35.58
PGSC0003DMT400057680	Potato	extracellular, plasma membrane, vacuole	37.3	35.57
AT2G34810.1	Thale cress	vacuole	37.5	35.56
KRH71761	Soybean	mitochondrion, plastid, vacuole	29.49	35.53
TraesCS1B01G448900.1	Wheat	endoplasmic reticulum, extracellular, vacuole	39.26	35.51
AT5G44380.2	Thale cress	mitochondrion, plastid	40.23	35.46
Solyc02g079440.1.1	Tomato	nucleus	37.11	35.32
PGSC0003DMT400036039	Potato	extracellular, plasma membrane, vacuole	38.09	35.2
Solyc02g079450.1.1	Tomato	extracellular	36.91	35.2
PGSC0003DMT400057523	Potato	extracellular, golgi, vacuole	35.94	32.68
Bra033739.1-P	Field mustard	plasma membrane, vacuole	38.67	32.57
Solyc02g079480.1.1	Tomato	plasma membrane, vacuole	35.35	32.26
CDX85018	Canola	cytosol	40.82	31.19
KRG99206	Soybean	extracellular, peroxisome	23.24	29.46

Protein Annotations

Gene3D:3.30.43.10	Gene3D:3.30.465.50	Gene3D:3.40.462.20	MapMan:50.1.1	InterPro:BBE	InterPro:FAD-bd_2
InterPro:FAD-bd_2-like_sf	InterPro:FAD-bd_2_sub1	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0005488
GO:GO:0008150	GO:GO:0008152	GO:GO:0016491	GO:GO:0016614	GO:GO:0050660	GO:GO:0055114
InterPro:IPR016166	InterPro:IPR016167	InterPro:Oxid_FAD_bind_N	PFAM:PF01565	PFAM:PF08031	PFscan:PS51387
PANTHER:PTHR32448	PANTHER:PTHR32448:SF23	SUPFAM:SSF56176	SignalP:SignalP-noTM	EnsemblPlantsGene:TraesCS4B01G391900	EnsemblPlants:TraesCS4B01G391900.1
SEG:seg	:	:	:	:	:

Description

No Description!

Coordinates

chr4B:+:667426580..667428120

Molecular Weight (calculated)

54971.8 Da

IEP (calculated)

8.200

GRAVY (calculated)

0.062

Length

512 amino acids

Sequence
(BLAST)

001: MLRVRGCLAL AVSVSFFFPC SVASPADAFL RCLSAKIPGE LVYTQSSTNF TDVLSSSIRN TKFLTNATAR PLCIVTPSDA SHVQAAVLCG RGQGVRLRVR
101: SGGHDYEGLS YRSVRPSEVF AVVDLGANLR AVRVNRLDST AWVDSGATIG ELYYAIAKND SRIAFPAGEC PTIGVGGHFS GGAIGMMMRK HGLSADVLDA
201: TLVDADGRLL DRAGMGEDLF WAIRGGGGGN FGIVLSWKVQ LVQVPPTVAV FNIAKTVDQG AVDILTRWQH VAPSLPGDLT IRVIVQGQQA MFQALYLGAC
301: GSLVATMGAQ FPELGMTSAE CQPMTWLQSA ATPFISFGRN GTLEEALLSR TAGQSGPGKI KSDYVRRAIP KAAWEEIFSW FTKDGAGFIL LEPHGGFMGS
401: VPAAATPYPH RHGVLYVIQY IAFWQQGGDG AAATAWIGGL YDLMGQHVSK NPRRAYVNFR DLDIGQNDGT PKSGEVWGER YFVGNYWRLA AVKAAVDPTD
501: YFSSEQSIPP LR

Best Arabidopsis Sequence Match ( AT4G20820.1 )
(BLAST)

001: MKFQSFFSSV LIFFTTSTLL LSIPHPVSAN RSNQSSFLQC LSLQLNDSNI VSKVIHTPND TSFSSVLASS IQNQRFSAPD VPKPVLILTP VQPSDVQSAV
101: KCARRFGIHI RTRSGGHDYE GLSYVTHKPF VILDLRNLRS ITVDVDNRSV WVQTGATIGE LYYEIGKKNR TLAFPAGVCP TVGVGGHFSG GGYGTLLRKH
201: GLAADHVIDA RVVDARGRIL ERREMGEDFF WAIRGGGGSS FCVVLSWKIG LINVPSTVTV FNVTKFSEQS ALKIIHRWQF VADKVSDDLF IRVMLQRYKN
301: MVRASFPGLY LGSVKNLLKM VNKEFPELGL EEDDCTEMSW IESVIWFAEL GEEPINVLTK RTRASLAFKA KSDFVQEPMP KTAISKLWRR LQEPEAEHAQ
401: LIFTPFGGKM SEIADYETPF PHRKGNIYEI QYLNYWRGDV KEKYMRWVER VYDDMSEFVA KSPRGAYINL RDLDLGMYVG VKRSKYEEGK SWGVKYFKNN
501: FERLVRVKTS VDPSDFFCDE QSIPPFTFVE VI

Arabidopsis Description

Berberine bridge enzyme-like 18 [Source:UniProtKB/Swiss-Prot;Acc:Q9SVG5]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.