Factsheet for TraesCS7B01G273700.1

Subcellular Localization

min:

: max

Winner_takes_all: vacuole

Predictor Summary:

extracellular 4
endoplasmic reticulum 4
vacuole 7
plasma membrane 4
golgi 4

Predictors

GFP

MS/MS

Papers

Winner Takes All:	vacuole
Any Predictor:	secretory
iPSORT:	secretory
MultiLoc:	vacuole
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	vacuole
YLoc:	vacuole

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
TraesCS7D01G368800.1	Wheat	vacuole	96.15	96.15
TraesCS7A01G353900.1	Wheat	vacuole	95.19	95.38
TraesCS4B01G391900.1	Wheat	vacuole	73.27	74.41
TraesCS4B01G104000.1	Wheat	vacuole	77.5	74.08
TraesCS2B01G572400.1	Wheat	unclear	74.04	72.64
TraesCS4B01G358600.1	Wheat	golgi	71.92	71.51
Os06t0549300-00	Rice	extracellular	69.81	69.41
EER89908	Sorghum	plasma membrane, vacuole	71.15	68.65
Zm00001d037008_P001	Maize	golgi, vacuole	66.73	67.91
TraesCS7B01G273600.1	Wheat	vacuole	65.0	65.38
TraesCS7B01G273500.1	Wheat	plastid	61.35	58.75
TraesCS7B01G026000.1	Wheat	cytosol	37.31	58.43
TraesCS3B01G132200.1	Wheat	extracellular, plasma membrane, vacuole	59.04	58.14
TraesCS2B01G066900.1	Wheat	nucleus, unclear	58.85	58.06
TraesCS5B01G302700.1	Wheat	vacuole	57.88	57.88
TraesCS2B01G066800.1	Wheat	vacuole	58.08	57.2
TraesCS2B01G066400.1	Wheat	plastid	56.73	55.87
TraesCS2B01G145300.1	Wheat	plasma membrane	59.42	54.02
TraesCS3B01G070000.1	Wheat	mitochondrion	29.23	53.9
TraesCS7B01G273800.1	Wheat	plasma membrane	53.85	53.74
TraesCS7B01G273900.1	Wheat	vacuole	53.65	53.65
TraesCS7B01G388200.1	Wheat	extracellular, plasma membrane, vacuole	53.85	53.64
TraesCS2B01G017300.1	Wheat	plastid	41.35	52.57
TraesCS2B01G145500.1	Wheat	vacuole	56.54	50.52
TraesCS2B01G172300.1	Wheat	vacuole	51.35	50.47
TraesCS2B01G016900.1	Wheat	vacuole	49.04	49.23
TraesCS1B01G456900.1	Wheat	vacuole	46.92	49.0
TraesCS1B01G469800.1	Wheat	vacuole	50.38	48.7
PGSC0003DMT400080465	Potato	cytosol	37.31	47.78
KRH36852	Soybean	plastid	39.62	46.5
VIT_03s0017g01470.t01	Wine grape	vacuole	27.88	46.33
CDY59834	Canola	peroxisome, plasma membrane, plastid	39.42	45.66
CDY66956	Canola	peroxisome, plasma membrane, plastid	36.92	45.61
GSMUA_Achr2P15490_001	Banana	golgi, vacuole	36.73	45.58
Solyc06g065060.1.1	Tomato	extracellular	46.92	45.35
CDY72595	Canola	extracellular, vacuole	33.08	45.26
Solyc10g054480.1.1	Tomato	plastid	26.35	45.07
CDY68515	Canola	extracellular, plasma membrane	32.88	45.0
Solyc06g065070.1.1	Tomato	vacuole	46.35	44.96
Bra024719.1-P	Field mustard	peroxisome, plastid, vacuole	39.04	44.91
PGSC0003DMT400080468	Potato	vacuole	45.58	44.89
PGSC0003DMT400080467	Potato	vacuole	46.15	44.61
KRH42273	Soybean	nucleus	45.19	44.59
KRH58389	Soybean	nucleus	45.77	44.4
KRH42274	Soybean	mitochondrion, nucleus	44.62	44.36
KRH58398	Soybean	plasma membrane, vacuole	45.19	44.26
KRH42266	Soybean	vacuole	45.77	44.16
VIT_10s0003g04730.t01	Wine grape	plasma membrane, vacuole	45.38	44.11
CDY29118	Canola	cytosol, peroxisome, plastid	33.08	44.1
VIT_10s0003g05550.t01	Wine grape	vacuole	44.81	43.88
CDX77574	Canola	cytosol	33.65	43.75
VIT_10s0003g05390.t01	Wine grape	extracellular	45.0	43.74
VIT_10s0003g05620.t01	Wine grape	vacuole	44.62	43.69
KRH42268	Soybean	vacuole	44.42	43.67
PGSC0003DMT400046308	Potato	extracellular	45.58	43.57
CDY69093	Canola	vacuole	44.81	43.55
KRH42808	Soybean	extracellular, vacuole	44.81	43.55
VIT_10s0003g05580.t01	Wine grape	plasma membrane, vacuole	44.42	43.5
KRH11824	Soybean	nucleus	45.38	43.46
KRH58399	Soybean	nucleus	44.62	43.45
KRH58391	Soybean	vacuole	44.23	43.4
PGSC0003DMT400054391	Potato	cytosol, nucleus, peroxisome	35.96	43.39
CDY29117	Canola	vacuole	44.62	43.36
Bra016272.1-P	Field mustard	peroxisome, plasma membrane, plastid	28.08	43.32
Bra027549.1-P	Field mustard	peroxisome	37.31	43.3
Bra023122.1-P	Field mustard	vacuole	44.04	43.21
AT5G44400.1	Thale cress	golgi, vacuole	44.62	43.2
KRH36842	Soybean	vacuole	44.23	43.15
CDY02321	Canola	extracellular, vacuole	44.42	43.1
CDY09546	Canola	extracellular, vacuole	44.23	42.91
VIT_12s0028g01780.t01	Wine grape	plasma membrane, vacuole	44.23	42.91
KRH11844	Soybean	vacuole	44.62	42.88
Solyc02g022850.1.1	Tomato	vacuole	44.62	42.88
CDX98676	Canola	vacuole	44.42	42.86
KRH58388	Soybean	vacuole	45.0	42.78
KRH36766	Soybean	vacuole	43.85	42.78
Bra019831.1-P	Field mustard	plasma membrane, vacuole	43.65	42.75
CDX97984	Canola	plasma membrane, vacuole	43.65	42.75
CDY31432	Canola	vacuole	43.65	42.75
KRH36839	Soybean	nucleus	44.62	42.73
KRH42267	Soybean	vacuole	43.27	42.69
CDY49803	Canola	vacuole	43.46	42.64
KRH58387	Soybean	plastid, vacuole	45.0	42.62
CDX79034	Canola	plastid	42.69	42.61
Bra033740.1-P	Field mustard	golgi	44.81	42.6
KRH36843	Soybean	vacuole	43.65	42.59
Bra014886.1-P	Field mustard	extracellular	43.08	42.59
KRH42269	Soybean	vacuole	43.46	42.56
Bra027552.1-P	Field mustard	peroxisome	27.88	42.52
AT5G44360.2	Thale cress	golgi	44.81	42.52
GSMUA_Achr4P31040_001	Banana	vacuole	43.65	42.51
Bra038798.1-P	Field mustard	vacuole	44.04	42.49
KRH36853	Soybean	vacuole	43.85	42.46
Bra020915.1-P	Field mustard	vacuole	43.27	42.37
VIT_10s0003g05470.t01	Wine grape	vacuole	45.96	42.3
CDX76449	Canola	vacuole	43.27	42.29
CDX94785	Canola	vacuole	43.65	42.27
AT4G20860.1	Thale cress	vacuole	43.08	42.26
CDY50728	Canola	extracellular, vacuole	42.69	42.21
VIT_12s0028g01790.t01	Wine grape	plastid	44.23	42.2
KRH36840	Soybean	nucleus	44.42	42.15
CDX82800	Canola	extracellular	42.31	42.15
Bra023131.1-P	Field mustard	endoplasmic reticulum, plasma membrane, vacuole	43.27	42.13
CDY02326	Canola	endoplasmic reticulum, plasma membrane, vacuole	43.27	42.13
CDY16155	Canola	endoplasmic reticulum, extracellular, vacuole	42.12	42.12
AT4G20840.1	Thale cress	plasma membrane	43.65	42.12
CDX94009	Canola	vacuole	43.65	42.12
Bra013501.1-P	Field mustard	vacuole	43.08	42.11
KRH11826	Soybean	nucleus	44.04	42.1
Bra028530.1-P	Field mustard	plasma membrane, vacuole	43.46	42.09
CDX67534	Canola	plasma membrane, vacuole	43.46	42.09
PGSC0003DMT400054387	Potato	plasma membrane, vacuole	42.88	42.08
CDY71012	Canola	extracellular, vacuole	42.69	42.05
KRH62528	Soybean	endoplasmic reticulum, golgi, vacuole	42.69	42.05
Solyc02g070060.1.1	Tomato	vacuole	42.5	42.02
AT1G30740.2	Thale cress	vacuole	43.27	41.98
CDY29122	Canola	cytosol	15.58	41.97
Bra013478.1-P	Field mustard	endoplasmic reticulum, vacuole	42.12	41.95
AT1G30720.1	Thale cress	vacuole	42.5	41.94
CDY09547	Canola	extracellular, vacuole	42.5	41.94
KRH42272	Soybean	vacuole	43.46	41.93
KRH58344	Soybean	vacuole	42.69	41.89
CDY70228	Canola	cytosol, golgi, peroxisome	24.81	41.88
KRH11845	Soybean	vacuole	43.08	41.87
Bra006964.1-P	Field mustard	endoplasmic reticulum, extracellular, vacuole	43.08	41.79
PGSC0003DMT400043091	Potato	extracellular	42.5	41.78
AT1G01980.1	Thale cress	plasma membrane, vacuole	43.46	41.77
KRH56394	Soybean	vacuole	42.31	41.75
Bra013476.1-P	Field mustard	cytosol, plastid	43.27	41.74
Bra033743.1-P	Field mustard	vacuole	43.08	41.71
CDY13353	Canola	plasma membrane	43.08	41.71
CDY49011	Canola	vacuole	43.08	41.71
TraesCS4B01G222500.1	Wheat	plasma membrane	45.38	41.7
PGSC0003DMT400086243	Potato	vacuole	42.12	41.63
CDX82785	Canola	endoplasmic reticulum, extracellular, vacuole	41.73	41.57
VIT_10s0003g05420.t01	Wine grape	vacuole	43.08	41.56
AT4G20820.1	Thale cress	plastid	42.5	41.54
Solyc09g072660.2.1	Tomato	extracellular	43.85	41.53
AT1G26390.1	Thale cress	vacuole	42.31	41.51
PGSC0003DMT400054564	Potato	cytosol, plastid	39.42	41.5
Bra023126.1-P	Field mustard	extracellular, vacuole	42.12	41.48
VIT_10s0003g05570.t01	Wine grape	plasma membrane, vacuole	44.42	41.47
CDY69774	Canola	endoplasmic reticulum, extracellular, plastid, vacuole	41.54	41.38
AT1G30700.1	Thale cress	vacuole	41.92	41.37
PGSC0003DMT400062414	Potato	plasma membrane, vacuole	44.04	41.34
KRH41927	Soybean	nucleus	42.12	41.32
Solyc02g070110.1.1	Tomato	extracellular, vacuole	42.12	41.32
PGSC0003DMT400089776	Potato	golgi, vacuole	43.46	41.32
AT1G26380.1	Thale cress	endoplasmic reticulum	42.5	41.31
AT5G44410.1	Thale cress	golgi	42.5	41.31
Bra013475.1-P	Field mustard	golgi, vacuole	42.5	41.31
CDX79007	Canola	golgi, vacuole	42.5	41.31
AT1G11770.1	Thale cress	vacuole	42.5	41.23
PGSC0003DMT400054385	Potato	plastid	39.23	41.21
AT1G26420.1	Thale cress	vacuole	41.92	41.21
CDX77568	Canola	endoplasmic reticulum, plasma membrane	42.31	41.2
CDX82776	Canola	endoplasmic reticulum, vacuole	42.31	41.2
Solyc02g070090.1.1	Tomato	extracellular	42.31	41.2
KRH11841	Soybean	vacuole	44.23	41.14
VIT_03s0017g01490.t01	Wine grape	vacuole	43.08	41.1
Bra013473.1-P	Field mustard	vacuole	41.54	41.06
Solyc02g070070.1.1	Tomato	extracellular	41.92	41.05
AT1G34575.1	Thale cress	vacuole	41.54	40.99
CDY43653	Canola	golgi	24.04	40.98
AT5G44390.1	Thale cress	vacuole	42.69	40.96
Solyc02g069690.1.1	Tomato	golgi, vacuole	43.08	40.95
Bra038799.1-P	Field mustard	golgi	42.12	40.93
CDX94008	Canola	golgi	42.12	40.93
Bra027547.1-P	Field mustard	vacuole	42.5	40.93
CDY13105	Canola	vacuole	42.5	40.93
PGSC0003DMT400054571	Potato	cytosol	31.92	40.89
CDX79005	Canola	vacuole	41.35	40.87
KRH41928	Soybean	vacuole	41.73	40.87
CDX84280	Canola	plasma membrane	40.0	40.86
Bra014890.1-P	Field mustard	plasma membrane	41.92	40.82
CDY67309	Canola	endoplasmic reticulum, plasma membrane	41.92	40.82
PGSC0003DMT400054393	Potato	plasma membrane, vacuole	41.92	40.82
KRH11827	Soybean	vacuole	42.12	40.78
VIT_03s0017g01420.t01	Wine grape	cytosol, peroxisome, vacuole	43.27	40.76
CDX82775	Canola	extracellular, vacuole	41.35	40.72
CDX94788	Canola	vacuole	41.92	40.67
CDY31642	Canola	plasma membrane	39.81	40.67
KRH56395	Soybean	nucleus	41.35	40.64
Bra005392.1-P	Field mustard	plasma membrane	41.54	40.6
PGSC0003DMT400054386	Potato	vacuole	41.54	40.6
CDX98675	Canola	golgi	41.73	40.56
CDX85019	Canola	extracellular	40.38	40.54
AT4G20800.1	Thale cress	endoplasmic reticulum, extracellular, vacuole	41.15	40.53
CDY49007	Canola	vacuole	42.12	40.48
Bra022992.1-P	Field mustard	mitochondrion	37.12	40.46
CDX79582	Canola	mitochondrion	37.12	40.46
CDY70640	Canola	mitochondrion	37.12	40.46
Solyc02g070130.1.1	Tomato	vacuole	41.35	40.34
CDX79008	Canola	vacuole	44.04	40.32
AT1G30730.1	Thale cress	extracellular	40.77	40.3
VIT_10s0003g05450.t01	Wine grape	extracellular	44.42	40.24
AT1G30760.2	Thale cress	golgi	42.69	40.22
VIT_12s0028g01770.t01	Wine grape	plasma membrane, vacuole	44.04	40.18
AT1G30710.1	Thale cress	extracellular, plasma membrane, vacuole	40.96	40.11
AT1G26400.1	Thale cress	golgi, vacuole	40.58	40.04
AT2G34790.1	Thale cress	golgi, vacuole	40.96	40.04
CDX82782	Canola	vacuole	43.85	40.0
KRH36851	Soybean	vacuole	43.46	40.0
Bra027555.1-P	Field mustard	extracellular, vacuole	40.96	39.96
Bra024717.1-P	Field mustard	vacuole	40.38	39.85
CDY16156	Canola	vacuole	40.38	39.85
KRH11830	Soybean	vacuole	37.69	39.84
Solyc02g070170.1.1	Tomato	extracellular, vacuole	40.96	39.81
CDX94780	Canola	plasma membrane, vacuole	41.54	39.78
CDY13104	Canola	extracellular, vacuole	40.77	39.77
CDY29116	Canola	extracellular, vacuole	40.77	39.77
PGSC0003DMT400054574	Potato	mitochondrion, peroxisome	39.23	39.77
CDX79010	Canola	cytosol	20.38	39.7
Solyc02g070200.1.1	Tomato	vacuole	41.15	39.56
CDY34627	Canola	vacuole	40.58	39.44
TraesCS3B01G406900.1	Wheat	vacuole	40.38	39.4
Bra014885.1-P	Field mustard	plasma membrane, vacuole	40.0	39.17
CDX77576	Canola	plasma membrane, vacuole	40.0	39.17
CDY50727	Canola	plasma membrane, vacuole	40.0	39.17
KRH49946	Soybean	extracellular, vacuole	31.92	39.15
AT1G26410.1	Thale cress	golgi	41.54	39.13
KRH42276	Soybean	nucleus	44.62	39.12
CDY52527	Canola	vacuole	40.77	39.11
KRH11825	Soybean	vacuole	39.23	39.08
Solyc02g070180.1.1	Tomato	plasma membrane, vacuole	40.58	39.07
CDY68914	Canola	golgi, plastid	32.5	39.03
KRH42271	Soybean	endoplasmic reticulum	43.27	38.99
Bra005390.1-P	Field mustard	vacuole	40.38	38.96
CDY69038	Canola	vacuole	40.38	38.96
AT4G20830.1	Thale cress	vacuole	42.69	38.95
Bra013472.1-P	Field mustard	extracellular, vacuole	39.42	38.9
Bra027553.1-P	Field mustard	vacuole	32.5	38.85
Bra031780.1-P	Field mustard	vacuole	40.19	38.85
CDY34829	Canola	vacuole	40.19	38.85
KRH58395	Soybean	endoplasmic reticulum	43.46	38.83
CDX79004	Canola	extracellular, vacuole	39.23	38.71
CDX82774	Canola	extracellular, vacuole	39.23	38.71
PGSC0003DMT400012979	Potato	cytosol	29.62	38.69
Bra023123.1-P	Field mustard	plasma membrane, vacuole	38.08	38.67
CDY02318	Canola	plasma membrane, vacuole	38.08	38.67
KRH12123	Soybean	plastid	33.85	38.43
PGSC0003DMT400054560	Potato	cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	26.73	38.29
Bra021930.1-P	Field mustard	plasma membrane	36.73	38.05
CDX94786	Canola	vacuole	38.65	37.85
PGSC0003DMT400036039	Potato	extracellular, plasma membrane, vacuole	40.19	37.73
PGSC0003DMT400057528	Potato	extracellular	38.85	37.55
Solyc02g079460.1.1	Tomato	extracellular, golgi, vacuole	38.08	37.22
AT5G44380.2	Thale cress	mitochondrion, plastid	41.54	37.18
AT2G34810.1	Thale cress	vacuole	38.46	37.04
Solyc02g079440.1.1	Tomato	nucleus	38.27	36.99
Solyc02g079470.1.1	Tomato	endoplasmic reticulum, vacuole	38.08	36.73
KRH71761	Soybean	mitochondrion, plastid, vacuole	30.0	36.71
KRH36841	Soybean	vacuole	27.69	36.64
CDY70679	Canola	cytosol, mitochondrion, peroxisome	16.35	36.48
AT5G44440.2	Thale cress	golgi	41.92	36.15
Solyc02g079450.1.1	Tomato	extracellular	37.31	36.13
PGSC0003DMT400057680	Potato	extracellular, plasma membrane, vacuole	37.12	35.94
CDX82780	Canola	cytosol	14.62	35.51
Bra033739.1-P	Field mustard	plasma membrane, vacuole	40.96	35.03
Solyc02g079480.1.1	Tomato	plasma membrane, vacuole	37.12	34.4
TraesCS1B01G448900.1	Wheat	endoplasmic reticulum, extracellular, vacuole	37.31	34.28
PGSC0003DMT400057523	Potato	extracellular, golgi, vacuole	36.73	33.93
CDX85018	Canola	cytosol	41.92	32.54
KRG99206	Soybean	extracellular, peroxisome	23.08	29.7

Protein Annotations

Gene3D:3.30.43.10	Gene3D:3.30.465.50	Gene3D:3.40.462.20	MapMan:50.1.1	InterPro:BBE	InterPro:FAD-bd_2
InterPro:FAD-bd_2-like_sf	InterPro:FAD-bd_2_sub1	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0005488
GO:GO:0008150	GO:GO:0008152	GO:GO:0016491	GO:GO:0016614	GO:GO:0050660	GO:GO:0055114
InterPro:IPR016166	InterPro:IPR016167	InterPro:Oxid_FAD_bind_N	PFAM:PF01565	PFAM:PF08031	PFscan:PS51257
PFscan:PS51387	PANTHER:PTHR32448	PANTHER:PTHR32448:SF23	SUPFAM:SSF56176	SignalP:SignalP-noTM	TMHMM:TMhelix
EnsemblPlantsGene:TraesCS7B01G273700	EnsemblPlants:TraesCS7B01G273700.1	SEG:seg	:	:	:

Description

No Description!

Coordinates

chr7B:+:502733926..502735488

Molecular Weight (calculated)

56720.7 Da

IEP (calculated)

6.059

GRAVY (calculated)

0.056

Length

520 amino acids

Sequence
(BLAST)

001: MAMLKGLALA LLVSFFSCYL ASVPSLASPD DFLQCLREKI PSELVYTKSS SSFAGVLVSS IRSARFFTNT TVRPLCVVTP TDASHVQAAV LCGRMQGVRL
101: RVRSGGHDYE GLSYRSARPE VFGVLDLANL RAVSVNQLES TAWVDSGATI GELYYTIAKD NSQLAFPAGL CPTIGVGGHF SGGAIGMMMR KYGLAVDNVL
201: DAKFVTANGD LLDRAGMGED LFWAIRGGGG GSFGIVLSWK VQLVQVPPTV TMFNIAKTLD QGAVDIVTRW QDVGPSLPND LTIRVIVQGQ QALFQALYLG
301: TCSSLVSTMG DQFPELTMTS ADCQSMTWLQ SIAFISFWNR DTPVEALLSR TTSLSTYTKS KSDYVQSAIS KGVWKDIFSW FTMNGAGLVI LEPHGGFMGS
401: VPTDATPYPH RSGVLYNVQY MVFWQGDGGT AANTWLGNFY DFMEQYVSKN PRQAYVNYRD LDIGQNVVVD DVTTFDGGKV WGEQYFASNF QRLASVKAAV
501: DPTDYFRNEQ SIPPLVQGRK

Best Arabidopsis Sequence Match ( AT5G44400.1 )
(BLAST)

001: MGISKPLPLF SILVLYFSLY TITPTSSLAS LQDQFINCVQ RNTHVYFPLE KTFFAPTKNV SMFSQVLEST AQNLRFLKKS MPKPGFIFSP IHESHVQASI
101: ICSKKLRMHL RVRSGGHDYE GLSYVSQIDK PFILMDLSKM RQVNINIQDN SAWVQSGATV GELYYRIAEK SKVHGFPAGL CSSLGIGGHI TGGAYGSMMR
201: KYGLGADNVL DAKIVDANGK LLDRAAMGED TFWAIRGGAG GSFGIILAWK IKLVPVPKTV TVFTVTKTLQ QDVGNKIISK WQRVADKLVE ELFIRVLFNV
301: AGTGGNKTVT TSYNALFLGG KGTLMNVMKK SFPELGLTFK DCIEMSWLES IAYISGFPTH TPTNVLLQGK SPFPKVSFKA KSDFVKTPIP ESGLQGIFKK
401: LLKEDIPLMI WNPYGGMMAK IPESQIPFPH RKGVLFKVQY VTSWLDSDKR PSRHINWIRD LYSYMTPYVS SNPREAYVNY RDLDLGRNTK DVKTCIKQAQ
501: VWGANYFKNN FNRLMMIKAK VDPENFFRHE QSIPPMM

Arabidopsis Description

Berberine bridge enzyme-like 26 [Source:UniProtKB/Swiss-Prot;Acc:Q9FKU8]
SUBAcon: [golgi,vacuole]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.