Subcellular Localization
min:
: max
Winner_takes_all: nucleus
Predictor Summary:
Predictor Summary:
- nucleus 3
- cytosol 1
- mitochondrion 1
| Predictors | GFP | MS/MS | Papers | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
PPI
No PPI Data
Homology
Paralog
| locus | Identity | Homology Identity |
|---|
Ortholog
| locus | Homology Species | Location | Identity | Homology Identity |
|---|---|---|---|---|
| CDX78309 | Canola | nucleus | 96.07 | 93.19 |
| CDY40774 | Canola | cytosol | 56.34 | 87.86 |
| CDX99029 | Canola | cytosol | 0.96 | 87.5 |
| CDY14029 | Canola | cytosol | 73.55 | 86.76 |
| PGSC0003DMT400063143 | Potato | cytosol | 16.87 | 78.03 |
| AT5G07740.2 | Thale cress | nucleus | 84.92 | 73.26 |
| Solyc03g115680.2.1 | Tomato | plastid | 26.1 | 72.88 |
| CDX99025 | Canola | cytosol | 58.26 | 72.56 |
| Solyc03g115670.1.1 | Tomato | cytosol, mitochondrion | 22.18 | 69.7 |
| CDX69994 | Canola | plastid | 56.34 | 68.91 |
| KRH03521 | Soybean | plastid | 32.02 | 68.69 |
| Zm00001d030781_P001 | Maize | cytosol | 4.75 | 64.49 |
| PGSC0003DMT400083163 | Potato | cytosol, mitochondrion, nucleus | 5.44 | 64.23 |
| TraesCS5D01G089100.1 | Wheat | cytosol | 13.15 | 62.42 |
| GSMUA_Achr7P26410_001 | Banana | endoplasmic reticulum, golgi | 20.66 | 61.73 |
| KRH54596 | Soybean | extracellular | 54.48 | 61.46 |
| VIT_17s0000g06640.t01 | Wine grape | cytosol, mitochondrion, plasma membrane, plastid | 55.79 | 60.95 |
| KRH56878 | Soybean | cytosol | 48.07 | 57.83 |
| KRH63288 | Soybean | cytosol | 36.02 | 55.29 |
| KRH03523 | Soybean | cytosol | 26.86 | 54.85 |
| Zm00001d030780_P001 | Maize | cytosol | 6.13 | 50.0 |
| Solyc07g005500.2.1 | Tomato | cytosol | 19.01 | 49.91 |
| CDY27130 | Canola | cytosol, mitochondrion | 13.5 | 49.87 |
| CDX69992 | Canola | cytosol | 25.83 | 49.54 |
| GSMUA_Achr3P30260_001 | Banana | mitochondrion, plasma membrane | 36.02 | 48.34 |
| CDX76779 | Canola | cytosol | 36.23 | 47.09 |
| CDY12361 | Canola | cytosol | 36.36 | 47.06 |
| GSMUA_Achr6P16780_001 | Banana | cytosol | 37.95 | 46.97 |
| CDY34742 | Canola | cytosol | 36.29 | 46.93 |
| CDY49153 | Canola | cytosol | 33.2 | 45.99 |
| CDX69993 | Canola | cytosol | 38.22 | 45.75 |
| CDX99026 | Canola | cytosol | 38.98 | 44.53 |
| CDY62689 | Canola | cytosol | 38.5 | 43.27 |
| CDY32721 | Canola | cytosol | 37.05 | 43.25 |
| CDY02799 | Canola | cytosol | 37.12 | 43.09 |
| Os07t0596300-02 | Rice | plastid | 48.28 | 43.09 |
| CDY02798 | Canola | cytosol | 14.39 | 42.65 |
| OQU90190 | Sorghum | cytosol | 48.07 | 42.51 |
| CDY12294 | Canola | cytosol | 35.67 | 42.49 |
| Zm00001d006758_P003 | Maize | cytosol | 45.87 | 41.09 |
| Zm00001d022040_P002 | Maize | cytosol, mitochondrion, nucleus, plasma membrane | 48.0 | 41.02 |
| CDY43877 | Canola | cytosol | 36.78 | 40.52 |
| GSMUA_Achr4P32680_001 | Banana | cytosol, mitochondrion | 42.7 | 35.51 |
| HORVU5Hr1G020180.51 | Barley | mitochondrion | 45.8 | 34.09 |
| KXG23793 | Sorghum | cytosol | 44.7 | 32.19 |
| Zm00001d041515_P003 | Maize | mitochondrion | 46.28 | 31.4 |
| CDX99027 | Canola | nucleus | 18.11 | 30.48 |
| Os04t0245000-00 | Rice | plasma membrane | 25.0 | 28.58 |
| CDY38795 | Canola | endoplasmic reticulum, golgi, plasma membrane | 8.88 | 20.54 |
| CDX94358 | Canola | mitochondrion, plastid | 2.13 | 19.87 |
| CDY22934 | Canola | nucleus, plastid | 9.78 | 19.48 |
| CDY05170 | Canola | extracellular, golgi, plasma membrane | 9.92 | 19.33 |
| CDY43029 | Canola | plasma membrane | 11.36 | 19.12 |
| CDY08280 | Canola | endoplasmic reticulum, golgi, plasma membrane | 10.47 | 19.07 |
| CDY42470 | Canola | plasma membrane | 11.23 | 18.98 |
| CDY70125 | Canola | cytosol | 6.27 | 18.96 |
| CDY06511 | Canola | endoplasmic reticulum, golgi, plasma membrane | 10.33 | 18.77 |
| CDY08720 | Canola | mitochondrion | 9.37 | 18.71 |
| CDY18346 | Canola | plasma membrane | 11.23 | 18.69 |
| CDY43578 | Canola | endoplasmic reticulum, extracellular | 10.81 | 18.69 |
| CDY43811 | Canola | golgi | 10.95 | 18.64 |
| CDY27766 | Canola | golgi, plasma membrane, vacuole | 10.19 | 18.59 |
| CDY36494 | Canola | endoplasmic reticulum, vacuole | 11.43 | 18.4 |
| CDY11614 | Canola | endoplasmic reticulum, plasma membrane, vacuole | 9.78 | 18.3 |
| CDY37849 | Canola | endoplasmic reticulum | 6.61 | 18.29 |
| CDY44259 | Canola | plasma membrane, vacuole | 10.4 | 17.91 |
| CDX79799 | Canola | mitochondrion, plastid, vacuole | 9.78 | 17.88 |
| CDY53556 | Canola | nucleus | 10.4 | 17.87 |
| CDX83457 | Canola | golgi, plasma membrane, vacuole | 10.33 | 17.79 |
| CDY14670 | Canola | endoplasmic reticulum, golgi, vacuole | 9.37 | 17.17 |
| CDY55061 | Canola | mitochondrion, nucleus | 8.75 | 17.07 |
| CDY40709 | Canola | endoplasmic reticulum, plasma membrane, vacuole | 9.57 | 17.01 |
| CDY17909 | Canola | golgi, vacuole | 9.85 | 16.86 |
| CDX74146 | Canola | extracellular, golgi, plasma membrane | 9.3 | 16.81 |
| CDY16239 | Canola | vacuole | 8.06 | 16.67 |
| CDY15092 | Canola | endoplasmic reticulum, plasma membrane, plastid, vacuole | 11.91 | 16.34 |
| CDY52291 | Canola | endoplasmic reticulum, plasma membrane, plastid, vacuole | 11.91 | 16.08 |
| CDY35941 | Canola | plastid | 8.13 | 15.75 |
| CDY31597 | Canola | mitochondrion | 6.89 | 15.72 |
| CDY28040 | Canola | plastid | 8.26 | 15.58 |
| CDY24501 | Canola | mitochondrion, nucleus | 8.61 | 15.45 |
| CDX74041 | Canola | golgi, plasma membrane, plastid | 7.44 | 15.15 |
| CDY33614 | Canola | mitochondrion, nucleus | 7.92 | 14.94 |
| CDY07990 | Canola | golgi, plasma membrane, plastid | 7.37 | 14.82 |
| CDX86310 | Canola | endoplasmic reticulum, plasma membrane | 7.09 | 12.76 |
| CDX85333 | Canola | plasma membrane | 7.23 | 12.76 |
Protein Annotations
| Gene3D:1.20.58.2220 | Gene3D:2.60.40.1110 | MapMan:20.2.2.6.2 | Gene3D:3.90.190.10 | EnsemblPlants:CDX81069 | ProteinID:CDX81069 |
| ProteinID:CDX81069.1 | ncoils:Coil | InterPro:FH2_Formin | EnsemblPlantsGene:GSBRNA2T00134510001 | InterPro:IPR014020 | InterPro:IPR015425 |
| InterPro:IPR029021 | InterPro:IPR029023 | PFAM:PF02181 | PFAM:PF10409 | PFscan:PS51181 | PFscan:PS51182 |
| PFscan:PS51444 | PANTHER:PTHR23213 | PANTHER:PTHR23213:SF271 | InterPro:Prot-tyrosine_phosphatase-like | SMART:SM00498 | SMART:SM01326 |
| SUPFAM:SSF101447 | SUPFAM:SSF49562 | SUPFAM:SSF52799 | InterPro:Tensin_C2-dom | InterPro:Tensin_phosphatase | UniParc:UPI0004EEFBF7 |
| SEG:seg | : | : | : | : | : |
Description
BnaC03g03230D
Coordinates
chrLK031823:-:1555650..1562575
Molecular Weight (calculated)
158373.0 Da
IEP (calculated)
7.394
GRAVY (calculated)
-0.619
Length
1452 amino acids
Sequence
(BLAST)
(BLAST)
0001: MALFRRFFYK KPPDHLLEIS ERLYVFDCCF SSDVMGEDEY RLYLGGIVAQ LQDHFPDASF MVFNFREGDQ RSQISDVLSQ YDMTVMDYPR HYESCPLLPL
0101: EMIHHFLKSS ESWLSLEGQQ NVLLMHCERG GWPVLAFMLS GLLLYRKQYQ GEQKTLEMVH KQAPKELLHL LSPLNPQPSQ LRYLQYISRR DLGSDWPPSD
0201: TPLLLDCLIL RDLPRFEGRE GCRPIVRVYG QDPKARANRS SVVLFSTPKT KKHTRLYQQE ECILVKLDIQ CRVQGDVVLE CIHLHDDLVR EEMIFRIMFH
0301: TAFVRANILI LQREEMDILW DAKDQFPKEF KAEVLFSGAD SVVPTIAIAP VSDDDENDFD MASPEEFYEV EEIFSDAVDG HELKRGDSDG FVVVDSASDD
0401: SEGKEVWKGD VEPNAFLDCA SDDSNHKHEA SADPVKDITV DDVQYRSDGT SIDSVKDIGI DDGDEQRKRR TMEAKENDSN TLSEKAQTKP RKQVGANAKL
0501: AGDALKPKSN VRMAKPNAVS RWIPSNKGSY KDSMHVAYPH TRINSAPASI TTSLKDGKRA TSPDGVITKD AKSKHLRASV SSPDVRSRAP SCLSPEFSPK
0601: EKPPSLPASP HYAPPLPQHP PSPPETASVL QPAASPPPPP PPPPTYSSPS QNDSNSQTSQ IPPPPPPPPF ASRRPNSGTM LPPPPPPPPL ASEKPKSGTG
0701: LPPLPLSPPW KSVYASALAT PKTCSTSQPP PPPQTSSHSQ TSQLPLPPPP PPPPPFASVR RNSETMLPPP PPPPPWKSVY TSTLETHEGS TSYNFPPPPP
0801: PLPPFSSSNT PKENGDHVAP SPLPYLSIAP SPSPKSSPIN GFSAPPPPFS KAHSVPVPPP PPPSYGSPTP PPPQPRSYGT PPPPPPFGKT SPPPPPPPPP
0901: FGSSCPPPPP PPPFRSGGPP PPPPPPMGAP PPPPPPMHRG APPPPPPPMH GGAPPPPPPP MPPPPPPPPM RGGAPPPPGG RVPGGPPPPP PPGGRAPGPP
1001: PPLGPRPPGG GPPPPPGPRP PGGGPPDLKG AGRGRGLSRP GLGSSAQKKS SLKPLHWVKV TRALQGSLWD ELQRQGQTAP EFDVSEIETL FSAIVPKPVD
1101: KSRKKSVGAK PEKVQLIDLR RANNTEIMLT KVKMPLPDMM AAVLAMDDTV LDSDQIENLI KFCPTKEEME LLKNYTGDKA VLGKCEQYFL ELMKVPRVES
1201: KMRVFSFKIQ FGTQITEFKK SLNAVNSACE EVRTSPKLKE IMKKILYLGN TLNQGTARGA AVGFKLDSLL KLSDTRAANS KMTLMHYLCK VLASQGSDLL
1301: DFPKDLESLE SASKIQLKSL AEEMQAIIKG LEKLNQELNA SESDGPVSEV FRKTLKDFIS VATTETAAVT SLYSVVGNNA DALAHYFGED PKRCPFEQVT
1401: ATLLNFTRLF KKAHEENIKQ EELERKKAAK EAEMEKEKAK GVSLTKTADD DS
0101: EMIHHFLKSS ESWLSLEGQQ NVLLMHCERG GWPVLAFMLS GLLLYRKQYQ GEQKTLEMVH KQAPKELLHL LSPLNPQPSQ LRYLQYISRR DLGSDWPPSD
0201: TPLLLDCLIL RDLPRFEGRE GCRPIVRVYG QDPKARANRS SVVLFSTPKT KKHTRLYQQE ECILVKLDIQ CRVQGDVVLE CIHLHDDLVR EEMIFRIMFH
0301: TAFVRANILI LQREEMDILW DAKDQFPKEF KAEVLFSGAD SVVPTIAIAP VSDDDENDFD MASPEEFYEV EEIFSDAVDG HELKRGDSDG FVVVDSASDD
0401: SEGKEVWKGD VEPNAFLDCA SDDSNHKHEA SADPVKDITV DDVQYRSDGT SIDSVKDIGI DDGDEQRKRR TMEAKENDSN TLSEKAQTKP RKQVGANAKL
0501: AGDALKPKSN VRMAKPNAVS RWIPSNKGSY KDSMHVAYPH TRINSAPASI TTSLKDGKRA TSPDGVITKD AKSKHLRASV SSPDVRSRAP SCLSPEFSPK
0601: EKPPSLPASP HYAPPLPQHP PSPPETASVL QPAASPPPPP PPPPTYSSPS QNDSNSQTSQ IPPPPPPPPF ASRRPNSGTM LPPPPPPPPL ASEKPKSGTG
0701: LPPLPLSPPW KSVYASALAT PKTCSTSQPP PPPQTSSHSQ TSQLPLPPPP PPPPPFASVR RNSETMLPPP PPPPPWKSVY TSTLETHEGS TSYNFPPPPP
0801: PLPPFSSSNT PKENGDHVAP SPLPYLSIAP SPSPKSSPIN GFSAPPPPFS KAHSVPVPPP PPPSYGSPTP PPPQPRSYGT PPPPPPFGKT SPPPPPPPPP
0901: FGSSCPPPPP PPPFRSGGPP PPPPPPMGAP PPPPPPMHRG APPPPPPPMH GGAPPPPPPP MPPPPPPPPM RGGAPPPPGG RVPGGPPPPP PPGGRAPGPP
1001: PPLGPRPPGG GPPPPPGPRP PGGGPPDLKG AGRGRGLSRP GLGSSAQKKS SLKPLHWVKV TRALQGSLWD ELQRQGQTAP EFDVSEIETL FSAIVPKPVD
1101: KSRKKSVGAK PEKVQLIDLR RANNTEIMLT KVKMPLPDMM AAVLAMDDTV LDSDQIENLI KFCPTKEEME LLKNYTGDKA VLGKCEQYFL ELMKVPRVES
1201: KMRVFSFKIQ FGTQITEFKK SLNAVNSACE EVRTSPKLKE IMKKILYLGN TLNQGTARGA AVGFKLDSLL KLSDTRAANS KMTLMHYLCK VLASQGSDLL
1301: DFPKDLESLE SASKIQLKSL AEEMQAIIKG LEKLNQELNA SESDGPVSEV FRKTLKDFIS VATTETAAVT SLYSVVGNNA DALAHYFGED PKRCPFEQVT
1401: ATLLNFTRLF KKAHEENIKQ EELERKKAAK EAEMEKEKAK GVSLTKTADD DS
0001: MALFRRFFYK KPPDRLLEIS ERVYVFDCCF SSDVMGEDEY KVYLGGIVAQ LQDHFPEASF MVFNFREGEQ RSQISDVLSQ YDMTVMDYPR QYESCPLLPL
0101: EMIHHFLRSS ESWLSLEGQQ NVLLMHCERG GWPVLAFMLS GLLLYRKQYH GEQKTLEMVH KQAPKELLHL LSPLNPQPSQ LRYLQYISRR NLGSDWPPSD
0201: TPLLLDCLIL RDLPHFEGKK GCRPILRVYG QDPKARTNRS SILLFSTLKT KKHTRLYQQE ECILVKLDIQ CRVQGDVVLE CIHLHDDLVS EEMVFRIMFH
0301: TAFVRANILM LQRDEMDILW DVKDQFPKEF KAEVLFSGAD AVVPPITTST LSDDENDFDM TSPEEFFEVE EIFSDVIDGP DHKRDSDSFV VVDTASDDSE
0401: GKEVWKGDVE PNAFLDCASD DSNHKHDMHA ETSTDPVKDI TVDDVQYRSD GKADSNIDSV KDIGIDDGDE QRKRRTVEAK ENDSSTVQTQ SKGDEESNDL
0501: ESMSQKTNTS LNKPISEKPQ ATLRKQVGAN AKPAAAGDSL KPKSKQQETQ GPNVRMAKPN AVSRWIPSNK GSYKDSMHVA YPPTRINSAP ASITTSLKDG
0601: KRATSPDGVI PKDAKTKYLR ASVSSPDMRS RAPICSSPDS SPKETPSSLP PASPHQAPPP LPSLTSEAKT VLHSSQAVAS PPPPPPPPPL PTYSHYQTSQ
0701: LPPPPPPPPP FSSERPNSGT VLPPPPPPPP PFSSERPNSG TVLPPPPPPP LPFSSERPNS GTVLPPPPSP PWKSVYASAL AIPAICSTSQ APTSSPTPPP
0801: PPPAYYSVGQ KSSDLQTSQL PSPPPPPPPP PFASVRRNSE TLLPPPPPPP PWKSLYASTF ETHEACSTSS SPPPPPPPPP FSPLNTTKAN DYILPPPPLP
0901: YTSIAPSPSV KILPLHGISS APSPPVKTAP PPPPPPPFSN AHSVLSPPPP SYGSPPPPPP PPPSYGSPPP PPPPPPSYGS PPPPPPPPPG YGSPPPPPPP
1001: PPSYGSPPPP PPPPFSHVSS IPPPPPPPPM HGGAPPPPPP PPMHGGAPPP PPPPPMHGGA PPPPPPPPMH GGAPPPPPPP MFGGAQPPPP PPMRGGAPPP
1101: PPPPMRGGAP PPPPPPMRGG APPPPPPPMH GGAPPPPPPP MRGGAPPPPP PPGGRGPGAP PPPPPPGGRA PGPPPPPGPR PPGGGPPPPP MLGARGAAVD
1201: PRGAGRGRGL PRPGFGSAAQ KKSSLKPLHW VKVTRALQGS LWDELQRHGE SQTPSEFDVS EIETLFSATV QKPADKSGSR RKSVGAKPEK VQLIDLRRAN
1301: NTEIMLTKVK MPLPDMMAAV LAMDESVLDV DQIENLIKFC PTKEEMELLK NYTGDKTTLG KCEQYFLELM KVPRVEAKLR VFSFKFQFGT QITEFKKSLN
1401: AVNSACEEVR SSQKLKEIMK KILYLGNTLN QGTARGAAVG FKLDSLSKLS DTRAANSKMT LMHYLCKVLA SKASVLLDFP KDLESLESAS KIQLKSLAEE
1501: MQAIIKGLEK LNQELTASES DGPVSDVFRK TLGDFISVAE TEVATVSSLY SVVGRNADAL AHYFGEDPNR CPFEQVTATL LNFIRLFKKA HEENVKQAEL
1601: EKKKALKEAE MEKAKGVNLT KKPVDDS
0101: EMIHHFLRSS ESWLSLEGQQ NVLLMHCERG GWPVLAFMLS GLLLYRKQYH GEQKTLEMVH KQAPKELLHL LSPLNPQPSQ LRYLQYISRR NLGSDWPPSD
0201: TPLLLDCLIL RDLPHFEGKK GCRPILRVYG QDPKARTNRS SILLFSTLKT KKHTRLYQQE ECILVKLDIQ CRVQGDVVLE CIHLHDDLVS EEMVFRIMFH
0301: TAFVRANILM LQRDEMDILW DVKDQFPKEF KAEVLFSGAD AVVPPITTST LSDDENDFDM TSPEEFFEVE EIFSDVIDGP DHKRDSDSFV VVDTASDDSE
0401: GKEVWKGDVE PNAFLDCASD DSNHKHDMHA ETSTDPVKDI TVDDVQYRSD GKADSNIDSV KDIGIDDGDE QRKRRTVEAK ENDSSTVQTQ SKGDEESNDL
0501: ESMSQKTNTS LNKPISEKPQ ATLRKQVGAN AKPAAAGDSL KPKSKQQETQ GPNVRMAKPN AVSRWIPSNK GSYKDSMHVA YPPTRINSAP ASITTSLKDG
0601: KRATSPDGVI PKDAKTKYLR ASVSSPDMRS RAPICSSPDS SPKETPSSLP PASPHQAPPP LPSLTSEAKT VLHSSQAVAS PPPPPPPPPL PTYSHYQTSQ
0701: LPPPPPPPPP FSSERPNSGT VLPPPPPPPP PFSSERPNSG TVLPPPPPPP LPFSSERPNS GTVLPPPPSP PWKSVYASAL AIPAICSTSQ APTSSPTPPP
0801: PPPAYYSVGQ KSSDLQTSQL PSPPPPPPPP PFASVRRNSE TLLPPPPPPP PWKSLYASTF ETHEACSTSS SPPPPPPPPP FSPLNTTKAN DYILPPPPLP
0901: YTSIAPSPSV KILPLHGISS APSPPVKTAP PPPPPPPFSN AHSVLSPPPP SYGSPPPPPP PPPSYGSPPP PPPPPPSYGS PPPPPPPPPG YGSPPPPPPP
1001: PPSYGSPPPP PPPPFSHVSS IPPPPPPPPM HGGAPPPPPP PPMHGGAPPP PPPPPMHGGA PPPPPPPPMH GGAPPPPPPP MFGGAQPPPP PPMRGGAPPP
1101: PPPPMRGGAP PPPPPPMRGG APPPPPPPMH GGAPPPPPPP MRGGAPPPPP PPGGRGPGAP PPPPPPGGRA PGPPPPPGPR PPGGGPPPPP MLGARGAAVD
1201: PRGAGRGRGL PRPGFGSAAQ KKSSLKPLHW VKVTRALQGS LWDELQRHGE SQTPSEFDVS EIETLFSATV QKPADKSGSR RKSVGAKPEK VQLIDLRRAN
1301: NTEIMLTKVK MPLPDMMAAV LAMDESVLDV DQIENLIKFC PTKEEMELLK NYTGDKTTLG KCEQYFLELM KVPRVEAKLR VFSFKFQFGT QITEFKKSLN
1401: AVNSACEEVR SSQKLKEIMK KILYLGNTLN QGTARGAAVG FKLDSLSKLS DTRAANSKMT LMHYLCKVLA SKASVLLDFP KDLESLESAS KIQLKSLAEE
1501: MQAIIKGLEK LNQELTASES DGPVSDVFRK TLGDFISVAE TEVATVSSLY SVVGRNADAL AHYFGEDPNR CPFEQVTATL LNFIRLFKKA HEENVKQAEL
1601: EKKKALKEAE MEKAKGVNLT KKPVDDS
Arabidopsis Description
actin binding [Source:TAIR;Acc:AT5G07740]
SUBAcon: [nucleus]
SUBAcon: [nucleus]
Hydropathy Plot
About CropPAL
The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.