CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: mitochondrion, nucleus

Predictor Summary:

plastid 2
nucleus 1
mitochondrion 2
cytosol 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 28499913
mitochondrion: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG91803
KRH34983
KRH34984

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G27850.1	KRG91803	AT1G35670.1	19130088
AT5G27850.1	KRH34983	AT1G35670.1	19130088
AT5G27850.1	KRH34984	AT1G35670.1	19130088

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH68491	Soybean	nucleus	81.42	98.4
KRH68205	Soybean	nucleus	79.2	95.72
KRH33871	Soybean	nucleus	79.2	95.72
KRG92914	Soybean	nucleus	79.2	95.72
KRG96455	Soybean	nucleus	78.76	95.19
VIT_14s0060g01930.t01	Wine grape	cytosol	75.66	91.44
GSMUA_Achr9P16300_001	Banana	cytosol, mitochondrion	36.28	90.11
PGSC0003DMT400063593	Potato	cytosol	71.24	86.1
GSMUA_Achr5P27080_001	Banana	cytosol	50.88	85.82
Solyc01g099900.2.1	Tomato	nucleus	70.8	85.56
GSMUA_Achr11P...	Banana	cytosol	50.44	85.07
AT3G05590.1	Thale cress	nucleus	69.91	84.49
AT5G27850.1	Thale cress	nucleus	69.91	84.49
Bra001156.1-P	Field mustard	nucleus	69.91	84.49
OQU92626	Sorghum	cytosol	69.47	83.96
Bra036611.1-P	Field mustard	cytosol	69.03	83.42
Bra009940.1-P	Field mustard	cytosol	69.03	83.42
EES17748	Sorghum	cytosol	69.03	82.98
EER97722	Sorghum	cytosol	68.58	82.89
Zm00001d009848_P001	Maize	cytosol	68.14	82.35
Zm00001d022462_P001	Maize	plastid	67.7	81.82
TraesCS4B01G166800.1	Wheat	cytosol	67.26	81.28
Os03t0341100-01	Rice	nucleus	66.81	80.75
TraesCS2A01G109900.1	Wheat	plastid	66.81	80.75
TraesCS4D01G169400.1	Wheat	cytosol, unclear	66.81	80.75
Os05t0155100-01	Rice	nucleus	66.81	80.32
TraesCS2B01G127900.1	Wheat	cytosol	66.37	80.21
TraesCS2D01G110100.1	Wheat	golgi, unclear	66.37	80.21
HORVU2Hr1G018820.1	Barley	cytosol	66.37	80.21
CDY05185	Canola	cytosol	21.24	80.0
CDY36892	Canola	cytosol	21.24	80.0
TraesCS1D01G105500.2	Wheat	golgi	66.37	79.79
TraesCS1B01G127200.1	Wheat	cytosol	65.93	79.68
TraesCS1A01G096500.2	Wheat	cytosol	65.93	79.68
GSMUA_Achr2P01730_001	Banana	cytosol	70.35	79.1
CDY25018	Canola	cytosol	68.58	78.68
CDY66803	Canola	cytosol	69.47	78.5
AT2G47570.1	Thale cress	cytosol, nucleus	64.16	77.96
Os07t0674700-01	Rice	plasma membrane	66.37	76.92
CDX99969	Canola	cytosol, plastid	70.8	76.19
CDX74138	Canola	extracellular	70.8	75.12
CDY19736	Canola	cytosol	71.24	74.88
HORVU1Hr1G022570.1	Barley	cytosol, plasma membrane	68.58	71.43
CDY40718	Canola	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, plasma membrane, plastid, vacuole	69.91	69.6
CDY05183	Canola	mitochondrion	67.26	68.16
HORVU2Hr1G018840.4	Barley	cytosol	27.43	68.13
CDX80616	Canola	cytosol	69.03	67.24
Zm00001d037603_P002	Maize	cytosol	68.14	66.96
CDY18813	Canola	cytosol, mitochondrion	71.68	65.06
GSMUA_Achr8P10280_001	Banana	cytosol	71.24	64.92
Bra040533.1-P	Field mustard	nucleus	70.35	61.39
Bra039423.1-P	Field mustard	cytosol	20.8	39.83
CDY27753	Canola	cytosol, mitochondrion	20.8	39.83
CDY46988	Canola	cytosol	14.6	39.76

Protein Annotations

MapMan:17.1.2.1.18	Gene3D:3.100.10.10	UniProt:A0A0R0F137	EMBL:ACUP02012162	EnsemblPlantsGene:GLYMA_19G232100	GO:GO:0003674
GO:GO:0003735	GO:GO:0005198	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737
GO:GO:0005840	GO:GO:0006412	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987
GO:GO:0019538	EnsemblPlants:KRG96775	ProteinID:KRG96775	ProteinID:KRG96775.1	InterPro:L18e/L15P_sf	PFAM:PF17135
PANTHER:PTHR10934	PANTHER:PTHR10934:SF9	InterPro:Ribosomal_L18e	InterPro:Ribosomal_L18e/L15P	SUPFAM:SSF52080	UniParc:UPI0006EE0438
SEG:seg	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr19:-:48242134..48244081

Molecular Weight (calculated)

25499.6 Da

IEP (calculated)

11.663

GRAVY (calculated)

-0.392

Length

226 amino acids

Sequence
(BLAST)

001: MRKARVHNKN PSMHSLNLRY SIDRSVVLEE VPFIALIAAM GIDLKAGGKS KKTKRTAPKS NDIYLKLLVK LYRFLVRRTD SNFNAVILKR LFMSKVNKPP
101: ISLSRLIKYT KGKEDKIAVV VGTVTDDIRV YEVPALKITA LKFTERARAR IEKAGGECLT FDQLALRAPL GQNTVLLRGP KNAREAVKHF GPAPGVPHSH
201: TKPYVRAKGR KFERARGRRN SRGFRV

Best Arabidopsis Sequence Match ( AT3G05590.1 )
(BLAST)

001: MGIDLIAGGK SKKTKRTAPK SDDVYLKLTV KLYRFLVRRT NSKFNGVILK RLFMSKVNKA PLSLSRLVEF MTGKEDKIAV LVGTITDDLR VHEIPAMKVT
101: ALRFTERARA RIEKAGGECL TFDQLALRAP LGQNTVLLRG PKNSREAVKH FGPAPGVPHS HSKPYVRAKG RKFEKARGKR KSRGFKV

Arabidopsis Description

RPL18BRPL18 [Source:UniProtKB/TrEMBL;Acc:A0A178V8M9]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.