CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, nucleus

Predictor Summary:

plastid 2
cytosol 4
mitochondrion 3

Predictors

GFP

MS/MS

Papers

endoplasmic reticulum: 23041469
nucleus: 27291164
endoplasmic reticulum: 28499913
nucleus: 28499913

msms PMID: 23041469 doi

S Komatsu, R Kuji, Y Nanjo, S Hiraga, K Furukawa

National Institute of Crop Science, Tsukuba 305-8518, Japan. skomatsu@affrc.go.jp

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27291164 doi

X Yin, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG91803
KRH34983
KRH34984

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G01250.1	KRG91803	AT4G09570.1	19130088
AT2G01250.1	KRH34983	AT4G09570.1	19130088
AT2G01250.1	KRH34984	AT4G09570.1	19130088

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH37351	Soybean	endoplasmic reticulum	86.76	97.52
OQU75637	Sorghum	cytosol	18.75	94.44
CDX95588	Canola	cytosol	18.01	90.74
KRH47287	Soybean	nucleus	79.04	88.48
KRH44630	Soybean	cytosol	78.68	88.07
PGSC0003DMT400051218	Potato	cytosol	75.74	85.12
Solyc07g008370.2.1	Tomato	cytosol, nucleus, plastid, unclear	75.74	85.12
PGSC0003DMT400075548	Potato	cytosol	74.63	84.58
AT2G01250.1	Thale cress	cytosol	75.0	84.3
AT3G13580.3	Thale cress	cytosol	75.37	84.02
CDY17884	Canola	cytosol, extracellular	74.63	83.88
Bra037665.1-P	Field mustard	cytosol	74.63	83.88
Bra004806.1-P	Field mustard	cytosol	74.63	83.88
PGSC0003DMT400053653	Potato	cytosol	75.37	83.67
CDX83440	Canola	cytosol, extracellular	74.26	83.13
CDX77810	Canola	cytosol, extracellular	73.9	83.06
CDY17686	Canola	cytosol	73.9	83.06
Bra024895.1-P	Field mustard	cytosol	73.9	83.06
CDY25941	Canola	cytosol	73.9	83.06
CDY37358	Canola	cytosol	73.9	83.06
CDY40393	Canola	cytosol	73.9	83.06
Bra017461.1-P	Field mustard	cytosol	73.9	83.06
Bra014334.1-P	Field mustard	cytosol	73.9	83.06
Bra000341.1-P	Field mustard	cytosol	59.56	82.65
CDY41074	Canola	cytosol	73.53	82.64
AT2G44120.2	Thale cress	cytosol	75.0	82.59
CDY30456	Canola	cytosol	75.37	79.77
Os08t0234000-01	Rice	nucleus	70.96	78.78
OQU75620	Sorghum	cytosol	70.22	77.96
KXG24783	Sorghum	cytosol	70.22	77.96
Zm00001d050193_P001	Maize	cytosol, plasma membrane, plastid	70.22	77.96
Zm00001d002395_P002	Maize	cytosol, extracellular	69.85	77.87
Os04t0605900-01	Rice	plastid	69.85	77.87
KXG27062	Sorghum	cytosol	66.91	77.78
Zm00001d024373_P001	Maize	cytosol	69.85	76.92
Zm00001d026254_P001	Maize	plastid	69.49	76.83
HORVU2Hr1G078780.1	Barley	cytosol	14.34	76.47
TraesCS2A01G432600.1	Wheat	cytosol, unclear	68.01	75.82
HORVU5Hr1G108990.2	Barley	cytosol	68.75	75.71
TraesCS5D01G462800.1	Wheat	cytosol, golgi	67.65	75.41
TraesCS5B01G461300.1	Wheat	cytosol	67.65	75.41
TraesCS5A01G452400.1	Wheat	nucleus	67.65	75.41
TraesCS2B01G453900.1	Wheat	cytosol, golgi, unclear	67.65	75.41
HORVU7Hr1G057320.1	Barley	cytosol	12.5	73.91
TraesCS2D01G430500.1	Wheat	golgi	66.18	73.77
KRH25929	Soybean	cytosol	23.53	73.56
Zm00001d030114_P001	Maize	cytosol	67.28	72.62
HORVU7Hr1G120140.1	Barley	extracellular, peroxisome	23.16	70.79
HORVU6Hr1G079960.1	Barley	cytosol, extracellular	23.9	67.01
CDY23419	Canola	extracellular	73.9	67.0
Zm00001d041838_P003	Maize	mitochondrion	30.51	66.4
HORVU2Hr1G100970.4	Barley	mitochondrion	66.54	61.99
CDY01953	Canola	cytosol	31.62	60.14
KXG27000	Sorghum	plastid	60.29	58.57
OQU80131	Sorghum	cytosol	70.22	57.53
KRG94901	Soybean	nucleus	40.44	46.61
CDX79781	Canola	extracellular	27.21	46.25
KRH06635	Soybean	cytosol	39.71	45.76
HORVU5Hr1G109020.1	Barley	extracellular	6.99	25.68

Protein Annotations

Gene3D:1.10.15.30	EntrezGene:100500011	MapMan:17.1.2.1.5	Gene3D:3.30.1390.20	EMBL:ACUP02009298	ncoils:Coil
EnsemblPlantsGene:GLYMA_15G167400	GO:GO:0000463	GO:GO:0002181	GO:GO:0003674	GO:GO:0003676	GO:GO:0003723
GO:GO:0003735	GO:GO:0005198	GO:GO:0005488	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005737	GO:GO:0005829	GO:GO:0005840	GO:GO:0006139	GO:GO:0006412	GO:GO:0008150
GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0019538	GO:GO:0022625	InterPro:IPR023106
InterPro:IPR036919	UniProt:K7MBV6	EnsemblPlants:KRH12348	ProteinID:KRH12348	ProteinID:KRH12348.1	InterPro:L30_ferredoxin-like_sf
PFAM:PF00327	PFAM:PF08079	ScanProsite:PS00634	PANTHER:PTHR11524	PANTHER:PTHR11524:SF16	InterPro:Ribosomal_L30_CS
InterPro:Ribosomal_L30_N	InterPro:Ribosomal_L30_central_dom_arc	InterPro:Ribosomal_L30_ferredoxin-like	InterPro:Ribosomal_L7_euk	InterPro:Ribosomal_L7_euk_arc	SUPFAM:SSF55129
TIGRFAMs:TIGR01310	UniParc:UPI000295C8E9	SEG:seg	:	:	:

Description

hypothetical protein

Coordinates

chr15:-:14778654..14781422

Molecular Weight (calculated)

31916.3 Da

IEP (calculated)

10.686

GRAVY (calculated)

-0.648

Length

272 amino acids

Sequence
(BLAST)

001: MENPIYKARR TPRTLTLRRR CKQSTTSIIQ MGEAKALVPE SVVKKEKRNE EWALAKKQEL DAAKKMRFET RKLIFNRAKQ YSKEYDEQQK ELIRLKREAK
101: LKGGFYVEPE AKLLFIIRIR GINAMDPKTR KILQLLRLRQ IFNGVFLKVN KATVNMLHRV EPYVTYGYPN LKSVRELIYK RGYGKVNKQR IALTDNSIIE
201: QTLGKHGIIC VEDLIHEIMT VGPHFKEANN FLWPFKLKAP LGGLEKKRNH YVEGGDAGNR EDYINELIRR MN

Best Arabidopsis Sequence Match ( AT3G13580.5 )
(BLAST)

001: MTEAESKTVV PESVLKKRKR EEEWALAKKQ ELEAAKKQNA EKRKLIFNRA KQYSKEYQEK ERELIQLKRE AKLKGGFYVD PEAKLLFIIR IRGINAIDPK
101: TKKILQLLRL RQIFNGVFLK VNKATINMLR RVEPYVTYGY PNLKSVKELI YKRGFGKLNH QRTALTDNSI VDQGLGKHGI ICVEDLIHEI MTVGPHFKEA
201: NNFLWPFQLK APLGGMKKKR NHYVEGGDAG NRENFINELV RRMN

Arabidopsis Description

RPL7DRibosomal protein L30/L7 family protein [Source:UniProtKB/TrEMBL;Acc:A0A178VHA2]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.