Factsheet for PGSC0003DMT400003356

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

plastid 9
mitochondrion 1

Predictors

GFP

MS/MS

Papers

cytosol: 22313117

msms PMID: 22313117 doi

S Lim, K Chisholm, RH Coffin, RD Peters, KI Al-Mughrabi, G Wang-Pruski, DM Pinto

Department of Plant and Animal Sciences, Nova Scotia Agricultural College, Truro, Nova Scotia, Canada.

PPI

Inferred distinct locusB in Crop

locusB	locations
PGSC0003DMT400001474
PGSC0003DMT400001475
PGSC0003DMT400001476
PGSC0003DMT400001613
PGSC0003DMT400015465
PGSC0003DMT400047487
PGSC0003DMT400069515

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G01180.1	PGSC0003DMT400001613	AT3G47620.1	21798944
AT3G01180.1	PGSC0003DMT400001474	AT3G49580.1	21798944
AT3G01180.1	PGSC0003DMT400001475	AT3G49580.1	21798944
AT3G01180.1	PGSC0003DMT400001476	AT3G49580.1	21798944
AT3G01180.1	PGSC0003DMT400069515	AT3G49580.1	21798944
AT3G01180.1	PGSC0003DMT400015465	AT5G13810.1	21798944
AT3G01180.1	PGSC0003DMT400047487	AT5G13810.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Solyc02g088000.2.1	Tomato	nucleus, plastid	96.48	96.35
KRH01182	Soybean	cytosol	28.68	82.71
CDY34695	Canola	plastid	14.99	80.42
Bra021486.1-P	Field mustard	endoplasmic reticulum	20.34	73.93
CDY37538	Canola	plastid	60.1	68.09
CDY43274	Canola	plastid	61.8	67.91
VIT_14s0108g00940.t01	Wine grape	plastid	68.19	65.38
KRH18473	Soybean	plastid	62.58	61.86
Bra039138.1-P	Field mustard	plastid	61.8	61.48
AT3G01180.1	Thale cress	plastid	63.1	61.11
KRG93537	Soybean	plastid	61.54	60.98
TraesCS6D01G287000.1	Wheat	plastid	51.5	59.58
TraesCS6B01G336400.1	Wheat	plastid	52.02	59.2
HORVU6Hr1G075950.4	Barley	plastid	52.02	59.11
Os02t0744700-01	Rice	nucleus	52.15	59.08
TraesCS6A01G307800.1	Wheat	plastid	51.89	58.88
EES05532	Sorghum	plastid	53.32	58.1
OQU91730	Sorghum	cytosol	51.63	57.89
Zm00001d052263_P001	Maize	plastid	52.15	57.14
Zm00001d018033_P001	Maize	plastid	50.72	56.54
CDY55806	Canola	plastid	40.16	55.7
Os10t0437600-00	Rice	plastid	53.45	54.74
TraesCS1B01G155700.3	Wheat	plastid	52.93	54.64
TraesCS1A01G137200.1	Wheat	plastid	53.32	54.46
TraesCS1D01G138100.2	Wheat	plastid	53.19	54.33
Zm00001d014150_P005	Maize	plastid	53.98	53.49
Zm00001d037234_P005	Maize	plastid	52.15	52.98
KXG19644	Sorghum	plastid	52.8	52.6
KRH15937	Soybean	plastid	8.74	51.54
TraesCS7A01G189000.1	Wheat	plastid	52.67	50.75
TraesCS7B01G093800.2	Wheat	plastid	52.8	50.75
Os06t0229800-01	Rice	plastid	53.45	50.62
HORVU1Hr1G035960.19	Barley	cytosol, plastid	52.8	50.44
TraesCS7D01G190100.1	Wheat	plastid	52.41	50.31
Bra021487.1-P	Field mustard	cytosol	23.86	49.33
HORVU7Hr1G038420.1	Barley	plastid	52.28	46.85
CDY34693	Canola	endoplasmic reticulum, golgi	22.56	43.47
PGSC0003DMT400047731	Potato	plastid	34.42	41.19
PGSC0003DMT400031568	Potato	plastid	27.9	35.26
PGSC0003DMT400035218	Potato	plastid	12.26	22.82
PGSC0003DMT400035217	Potato	plastid	9.52	21.41
PGSC0003DMT400078688	Potato	cytosol	10.3	20.26
PGSC0003DMT400021444	Potato	plastid	24.25	18.58

Protein Annotations

KEGG:00500+2.4.1.21	EntrezGene:102583115	MapMan:3.2.2.4.2	Gene3D:3.40.50.2000	ProteinID:CAA61241	ProteinID:CAA61241.2
ProteinID:CAA62438	ProteinID:CAA62438.1	ncoils:Coil	GO:GO:0003674	GO:GO:0003824	GO:GO:0004373
GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005975	GO:GO:0008150
GO:GO:0008152	GO:GO:0009011	GO:GO:0009058	GO:GO:0009501	GO:GO:0009507	GO:GO:0009536
GO:GO:0009987	GO:GO:0016740	GO:GO:0016757	GO:GO:0019252	GO:GO:0033201	InterPro:GS/SS
InterPro:Glyco_trans_1	HAMAP:MF_00484	RefSeq:NP_001274977.1	PFAM:PF00534	PFAM:PF08323	EnsemblPlantsGene:PGSC0003DMG400001328
PGSC:PGSC0003DMG400001328	EnsemblPlants:PGSC0003DMT400003356	PANTHER:PTHR12526	PANTHER:PTHR12526:SF542	UniProt:Q43847	SUPFAM:SSF53756
InterPro:Starch_synth_cat_dom	TIGRFAMs:TIGR02095	UniParc:UPI00003B0CBC	EMBL:X87988	EMBL:X90943	SEG:seg

Description

SS2Granule-bound starch synthase 2, chloroplastic/amyloplastic [Source:UniProtKB/Swiss-Prot;Acc:Q43847]

Coordinates

chr2:-:46189568..46196715

Molecular Weight (calculated)

85226.2 Da

IEP (calculated)

6.273

GRAVY (calculated)

-0.387

Length

767 amino acids

Sequence
(BLAST)

001: MENSILLHSG NQFHPNLPLL ALRPKKLSLI HGSSREQMWR IKRVKATGEN SGEAASADES NDALQVTIEK SKKVLAMQQD LLQQIAERRK VVSSIKSSLA
101: NAKGTYDGGS GSLSDVDIPD VDKDYNVTVP STAATPITDV DKNTPPAISQ DFVESKREIK RDLADERAPP LSRSSITASS QISSTVSSKR TLNVPPETPK
201: SSQETLLDVN SRKSLVDVPG KKIQSYMPSL RKESSASHVE QRNENLEGSS AEANEETEDP VNIDEKPPPL AGTNVMNIIL VASECAPWSK TGGLGDVAGA
301: LPKALARRGH RVMVVAPRYD NYPEPQDSGV RKIYKVDGQD VEVTYFQAFI DGVDFVFIDS HMFRHIGNNI YGGNRVDILK RMVLFCKAAI EVPWHVPCGG
401: VCYGDGNLVF IANDWHTALL PVYLKAYYRD NGIMNYTRSV LVIHNIAHQG RGPLEDFSYV DLPPHYMDPF KLYDPVGGEH FNIFAAGLKT ADRVVTVSHG
501: YSWELKTSQG GWGLHQIINE NDWKLQGIVN GIDTKEWNPE LDVHLQSDGY MNYSLDTLQT GKPQCKAALQ KELGLPVRDD VPLIGFIGRL DPQKGVDLIA
601: EAVPWMMGQD VQLVMLGTGR RDLEQMLRQF ECQHNDKIRG WVGFSVKTSH RITAGADILL MPSRFEPCGL NQLYAMKYGT IPVVHAVGGL RDTVQPFDPF
701: NESGLGWTFS RAEASQLIHA LGNCLLTYRE YKKSWEGIQT RCMTQDLSWD NAAQNYEEVL IAAKYQW

Best Arabidopsis Sequence Match ( AT3G01180.1 )
(BLAST)

001: MASVAESSFP LLCQIKTQRR INSSTLRHSR VSYHDLPSGS LSFRSRSFVL GHRCKCVSRV EASGSDDDEP EDALQATIDK SKKVLAMQRN LLHQIAERRK
101: LVSSIKESTP DLDDAKASSK QESASSVNAN TDATKKEIMD GDANGSVSPS TYGKSSLSKE PEAKTFSPST ESLKNRKQSS ASVISSSPVT SPQKPSDVAT
201: NGKPWSSVVA SSVDPPYKPS SVMTSPEKTS DPVTSPGKPS KSRAGAFWSD PLPSYLTKAP QTSTMKTEKY VEKTPDVASS ETNEPGKDEE KPPPLAGANV
301: MNVILVAAEC APFSKTGGLG DVAGALPKSL ARRGHRVMVV VPRYAEYAEA KDLGVRKRYK VAGQDMEVMY FHAFIDGVDF VFIDSPEFRH LSNNIYGGNR
401: LDILKRMVLF CKAAVEVPWY VPCGGVCYGD GNLAFIANDW HTALLPVYLK AYYRDHGIMK YTRSVLVIHN IAHQGRGPVD DFSYVDLPSH YLDSFKLYDP
501: VGGEHFNIFA AGLKAADRVL TVSHGYSWEV KTLEGGWGLH NIINENDWKF RGIVNGIDTQ EWNPEFDTYL HSDDYTNYSL ENLHIGKPQC KAALQKELGL
601: PVRPDVPLIG FIGRLDHQKG VDLIAEAVPW MMSQDVQLVM LGTGRPDLEE VLRQMEHQYR DKARGWVGFS VKTAHRITAG ADILLMPSRF EPCGLNQLYA
701: MNYGTIPVVH AVGGLRDTVQ QFDPYSETGL GWTFDSAEAG KLIHALGNCL LTYREYKESW EGLQRRGMTQ DLSWDNAAEK YEEVLVAAKY HW

Arabidopsis Description

SS2Starch synthase, chloroplastic/amyloplastic (Fragment) [Source:UniProtKB/TrEMBL;Acc:W8QNS2]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.