CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, nucleus

Predictor Summary:

plastid 2
mitochondrion 2
cytosol 4

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 23041469
extracellular: 25724908
endoplasmic reticulum: 27224218
mitochondrion: 27780359
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27780359 doi

G Mustafa, S Komatsu

Graduate School of Life and Environmental Science, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 23041469 doi

S Komatsu, R Kuji, Y Nanjo, S Hiraga, K Furukawa

National Institute of Crop Science, Tsukuba 305-8518, Japan. skomatsu@affrc.go.jp

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 25724908 doi

VC Clarke, PC Loughlin, A Gavrin, C Chen, EM Brear, DA Day, PM Smith

From the ‡University of Sydney, School of Biological Sciences, Sydney Australia;, From the ‡University of Sydney, School of Biological Sciences, Sydney Australia; penny.smith@sydney.edu.au., From the ‡University of Sydney, School of Biological Sciences, Sydney Australia; §Flinders University, School of Biological Sciences, Adelaide Australia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH16972	cytosol, cytosol, mitochondrion
KRH72603	cytosol, cytosol, mitochondrion

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G13440.1	KRH16972	AT2G36460.1	23316205
AT1G13440.1	KRH72603	AT2G36460.1	23316205
AT1G13440.1	KRH16972	AT2G36460.1	23316205
AT1G13440.1	KRH72603	AT2G36460.1	23316205
AT3G04120.1	KRH16972	AT2G36460.1	23316205
AT3G04120.1	KRH72603	AT2G36460.1	23316205
AT3G04120.1	KRH16972	AT2G36460.1	23316205
AT3G04120.1	KRH72603	AT2G36460.1	23316205

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH31426	Soybean	endoplasmic reticulum	98.53	98.53
VIT_01s0010g02460.t01	Wine grape	cytosol	91.47	91.47
AT3G04120.1	Thale cress	cytosol	90.88	91.42
Os04t0486600-01	Rice	plastid	90.59	91.39
AT1G13440.1	Thale cress	cytosol	90.59	91.12
Bra016729.1-P	Field mustard	cytosol	90.29	90.56
Bra026904.1-P	Field mustard	cytosol	89.71	90.24
CDY49358	Canola	cytosol, mitochondrion	89.12	90.18
Bra040213.1-P	Field mustard	cytosol	89.41	89.94
Bra019722.1-P	Field mustard	cytosol	77.65	89.8
Bra040146.1-P	Field mustard	cytosol	89.12	89.64
KRH63724	Soybean	nucleus	88.82	89.35
KRH54234	Soybean	endoplasmic reticulum, nucleus	88.82	89.35
KRH63725	Soybean	endoplasmic reticulum, nucleus	88.53	89.05
KRH54227	Soybean	endoplasmic reticulum, nucleus	88.24	88.76
TraesCS6D01G196300.2	Wheat	golgi, nucleus	87.35	88.13
Os02t0601300-01	Rice	plasma membrane	87.35	88.13
TraesCS6A01G213700.1	Wheat	unclear	87.06	87.83
Zm00001d051001_P002	Maize	cytosol	86.76	87.54
EES05377	Sorghum	cytosol	86.76	87.54
CDX92059	Canola	cytosol, endoplasmic reticulum, peroxisome	89.41	87.36
KXG21112	Sorghum	cytosol	86.47	87.24
KRG94350	Soybean	cytosol, mitochondrion	85.88	86.65
Os08t0126300-01	Rice	cytosol	85.88	86.65
KRH57621	Soybean	endoplasmic reticulum	85.88	86.65
KXG20851	Sorghum	cytosol	85.59	86.35
KXG24320	Sorghum	cytosol	85.59	86.35
TraesCS7B01G213300.1	Wheat	golgi	85.59	86.35
TraesCS7D01G309500.1	Wheat	cytosol	85.59	86.35
CDX74199	Canola	endoplasmic reticulum	89.12	86.08
Zm00001d049641_P001	Maize	plasma membrane, plastid	85.29	86.05
CDY42906	Canola	cytosol	89.12	85.84
OQU77090	Sorghum	cytosol	55.88	85.59
Zm00001d035156_P003	Maize	cytosol	85.29	83.09
CDX98100	Canola	cytosol, mitochondrion, peroxisome	77.65	83.02
TraesCS6B01G243700.1	Wheat	endoplasmic reticulum	87.35	82.96
CDY50169	Canola	cytosol	90.29	82.53
CDY21098	Canola	cytosol	90.29	82.31
TraesCS7A01G313100.1	Wheat	cytosol	83.24	82.03
TraesCS7B01G234400.1	Wheat	cytosol	82.35	81.63
HORVU6Hr1G054520.3	Barley	cytosol, mitochondrion	87.35	81.59
CDY26651	Canola	mitochondrion, peroxisome	77.35	78.98
CDY30032	Canola	cytosol	90.29	78.12
CDY21135	Canola	cytosol, mitochondrion, peroxisome, plastid	77.06	76.83
HORVU7Hr1G074690.3	Barley	extracellular, plasma membrane	85.59	74.05
CDY16090	Canola	cytoskeleton, cytosol, peroxisome	90.0	71.16
CDY29987	Canola	cytosol, peroxisome, plastid	76.76	69.41
KRH66238	Soybean	plastid	73.24	59.57
KRH07324	Soybean	extracellular, nucleus	72.65	59.09
Zm00001d017121_P001	Maize	plastid	86.76	57.84
KRH37937	Soybean	plastid	12.65	48.86
KRH08444	Soybean	cytosol	30.88	40.23
KRG94751	Soybean	extracellular, nucleus	47.06	39.7
KRH06774	Soybean	nucleus	46.76	39.45
KRH74912	Soybean	cytosol	16.18	39.29
KRH60895	Soybean	nucleus, plastid	49.41	37.25
KRH51576	Soybean	nucleus, plastid	49.41	37.09
KRH70104	Soybean	plastid	26.47	32.61
KRH26225	Soybean	mitochondrion	27.06	29.02

Protein Annotations

KEGG:00010+1.2.1.12	KEGG:00710+1.2.1.12	EntrezGene:100782924	MapMan:2.1.1.4.1	Gene3D:3.30.360.10	Gene3D:3.40.50.720
EMBL:ACUP02010799	EMBL:BT099150	UniProt:C6TN36	EnsemblPlantsGene:GLYMA_18G009700	GO:GO:0000166	GO:GO:0003674
GO:GO:0003824	GO:GO:0005488	GO:GO:0005975	GO:GO:0006006	GO:GO:0008150	GO:GO:0008152
GO:GO:0016491	GO:GO:0016620	GO:GO:0050661	GO:GO:0051287	GO:GO:0055114	InterPro:GlycerAld/Erythrose_P_DH
InterPro:GlycerAld_3-P_DH_AS	InterPro:GlycerAld_3-P_DH_NAD(P)-bd	InterPro:GlycerAld_3-P_DH_cat	InterPro:Glyceraldehyde-3-P_DH_1	EnsemblPlants:KRG97466	ProteinID:KRG97466
ProteinID:KRG97466.1	InterPro:NAD(P)-bd_dom_sf	PFAM:PF00044	PFAM:PF02800	PIRSF:PIRSF000149	PRINTS:PR00078
ScanProsite:PS00071	PANTHER:PTHR10836	PANTHER:PTHR10836:SF63	MetaCyc:PWY-1042	MetaCyc:PWY-5484	MetaCyc:PWY-6901
MetaCyc:PWY-7003	SMART:SM00846	SUPFAM:SSF51735	SUPFAM:SSF55347	TIGRFAMs:TIGR01534	UniParc:UPI0001B1732B
SEG:seg	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr18:-:691244..695517

Molecular Weight (calculated)

36998.3 Da

IEP (calculated)

7.611

GRAVY (calculated)

-0.100

Length

340 amino acids

Sequence
(BLAST)

001: MASDKKIRIG INGFGRIGRL VARVALQRND VELVAVNDPF ITTDYMTYMF KYDTVHGQWK HFDVKVKDSK TLLFGEKPVT VFGIRNPEEI PWGEVGADYV
101: VESTGVFTDK DKAAAHLKGG AKKVVISAPS KDAPMFVVGV NEKEYKPELD IVSNASCTTN CLAPLAKVIN DRFGIVEGLM TTVHAITATQ KTVDGPSNKD
201: WRGGRAASFN IIPSSTGAAK AVGKVLPALN GKLTGMSFRV PTVDVSVVDL TVRLEKPATY EQIKSAIKEE SEGKLKGILG YTEDDVVSTD FVGDNRSSIF
301: DAKAGISLND NFVKLVSWYD NEWGYSSRVI DLIVHIASVA

Best Arabidopsis Sequence Match ( AT3G04120.1 )
(BLAST)

001: MADKKIRIGI NGFGRIGRLV ARVVLQRDDV ELVAVNDPFI TTEYMTYMFK YDSVHGQWKH NELKIKDEKT LLFGEKPVTV FGIRNPEDIP WAEAGADYVV
101: ESTGVFTDKD KAAAHLKGGA KKVVISAPSK DAPMFVVGVN EHEYKSDLDI VSNASCTTNC LAPLAKVIND RFGIVEGLMT TVHSITATQK TVDGPSMKDW
201: RGGRAASFNI IPSSTGAAKA VGKVLPALNG KLTGMSFRVP TVDVSVVDLT VRLEKAATYD EIKKAIKEES EGKLKGILGY TEDDVVSTDF VGDNRSSIFD
301: AKAGIALSDK FVKLVSWYDN EWGYSSRVVD LIVHMSKA

Arabidopsis Description

GAPC1Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic [Source:UniProtKB/Swiss-Prot;Acc:P25858]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.