CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

plastid 2
mitochondrion 2
cytosol 4

Predictors

GFP

MS/MS

Papers

endoplasmic reticulum: 21107622
nucleus: 21132161
endoplasmic reticulum: 23041469
endoplasmic reticulum: 27224218
mitochondrion: 27780359
endoplasmic reticulum: 28499913
mitochondrion: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27780359 doi

G Mustafa, S Komatsu

Graduate School of Life and Environmental Science, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 21107622 doi

N Ahsan, T Nakamura, S Komatsu

National Institute of Crop Science, 2-1-18 Kannondai, Tsukuba, 305-8518, Japan.

msms PMID: 23041469 doi

S Komatsu, R Kuji, Y Nanjo, S Hiraga, K Furukawa

National Institute of Crop Science, Tsukuba 305-8518, Japan. skomatsu@affrc.go.jp

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH16972	cytosol, cytosol, mitochondrion
KRH72603	cytosol, cytosol, mitochondrion

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G13440.1	KRH16972	AT2G36460.1	23316205
AT1G13440.1	KRH72603	AT2G36460.1	23316205
AT1G13440.1	KRH16972	AT2G36460.1	23316205
AT1G13440.1	KRH72603	AT2G36460.1	23316205
AT3G04120.1	KRH16972	AT2G36460.1	23316205
AT3G04120.1	KRH72603	AT2G36460.1	23316205
AT3G04120.1	KRH16972	AT2G36460.1	23316205
AT3G04120.1	KRH72603	AT2G36460.1	23316205

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRG97466	Soybean	endoplasmic reticulum, nucleus	98.53	98.53
VIT_01s0010g02460.t01	Wine grape	cytosol	91.47	91.47
Os04t0486600-01	Rice	plastid	90.29	91.1
AT3G04120.1	Thale cress	cytosol	90.0	90.53
Bra016729.1-P	Field mustard	cytosol	90.0	90.27
Bra026904.1-P	Field mustard	cytosol	89.41	89.94
AT1G13440.1	Thale cress	cytosol	89.41	89.94
CDY49358	Canola	cytosol, mitochondrion	87.94	88.99
Bra019722.1-P	Field mustard	cytosol	76.76	88.78
Bra040213.1-P	Field mustard	cytosol	88.24	88.76
KRH63724	Soybean	nucleus	88.24	88.76
KRH54234	Soybean	endoplasmic reticulum, nucleus	88.24	88.76
Bra040146.1-P	Field mustard	cytosol	87.94	88.46
KRH63725	Soybean	endoplasmic reticulum, nucleus	87.94	88.46
KRH54227	Soybean	endoplasmic reticulum, nucleus	87.65	88.17
TraesCS6D01G196300.2	Wheat	golgi, nucleus	87.06	87.83
Os02t0601300-01	Rice	plasma membrane	87.06	87.83
TraesCS6A01G213700.1	Wheat	unclear	86.76	87.54
Zm00001d051001_P002	Maize	cytosol	86.76	87.54
EES05377	Sorghum	cytosol	86.76	87.54
KXG21112	Sorghum	cytosol	86.47	87.24
KRH57621	Soybean	endoplasmic reticulum	85.88	86.65
KRG94350	Soybean	cytosol, mitochondrion	85.59	86.35
TraesCS7B01G213300.1	Wheat	golgi	85.59	86.35
TraesCS7D01G309500.1	Wheat	cytosol	85.59	86.35
CDX92059	Canola	cytosol, endoplasmic reticulum, peroxisome	88.24	86.21
KXG20851	Sorghum	cytosol	85.29	86.05
KXG24320	Sorghum	cytosol	85.29	86.05
Os08t0126300-01	Rice	cytosol	85.0	85.76
Zm00001d049641_P001	Maize	plasma membrane, plastid	85.0	85.76
OQU77090	Sorghum	cytosol	55.88	85.59
CDX74199	Canola	endoplasmic reticulum	87.94	84.94
CDY42906	Canola	cytosol	87.94	84.7
Zm00001d035156_P003	Maize	cytosol	85.0	82.81
TraesCS6B01G243700.1	Wheat	endoplasmic reticulum	87.06	82.68
CDX98100	Canola	cytosol, mitochondrion, peroxisome	76.76	82.08
TraesCS7A01G313100.1	Wheat	cytosol	83.24	82.03
CDY50169	Canola	cytosol	89.71	81.99
CDY21098	Canola	cytosol	89.71	81.77
TraesCS7B01G234400.1	Wheat	cytosol	82.35	81.63
HORVU6Hr1G054520.3	Barley	cytosol, mitochondrion	87.06	81.32
CDY26651	Canola	mitochondrion, peroxisome	77.06	78.68
CDY30032	Canola	cytosol	89.41	77.35
CDY21135	Canola	cytosol, mitochondrion, peroxisome, plastid	75.88	75.66
HORVU7Hr1G074690.3	Barley	extracellular, plasma membrane	85.59	74.05
CDY16090	Canola	cytoskeleton, cytosol, peroxisome	89.71	70.93
CDY29987	Canola	cytosol, peroxisome, plastid	75.59	68.35
KRH66238	Soybean	plastid	73.53	59.81
KRH07324	Soybean	extracellular, nucleus	72.94	59.33
Zm00001d017121_P001	Maize	plastid	86.76	57.84
KRH37937	Soybean	plastid	12.94	50.0
KRH08444	Soybean	cytosol	30.88	40.23
KRG94751	Soybean	extracellular, nucleus	47.06	39.7
KRH06774	Soybean	nucleus	46.76	39.45
KRH74912	Soybean	cytosol	16.18	39.29
KRH60895	Soybean	nucleus, plastid	49.41	37.25
KRH51576	Soybean	nucleus, plastid	49.41	37.09
KRH70104	Soybean	plastid	26.47	32.61
KRH26225	Soybean	mitochondrion	27.35	29.34

Protein Annotations

KEGG:00010+1.2.1.12	KEGG:00710+1.2.1.12	EntrezGene:100807342	MapMan:2.1.1.4.1	Gene3D:3.30.360.10	Gene3D:3.40.50.720
EMBL:ACUP02007145	EMBL:BT095509	UniProt:C6TD56	EnsemblPlantsGene:GLYMA_11G247600	GO:GO:0000166	GO:GO:0003674
GO:GO:0003824	GO:GO:0005488	GO:GO:0005975	GO:GO:0006006	GO:GO:0008150	GO:GO:0008152
GO:GO:0016491	GO:GO:0016620	GO:GO:0050661	GO:GO:0051287	GO:GO:0055114	InterPro:GlycerAld/Erythrose_P_DH
InterPro:GlycerAld_3-P_DH_AS	InterPro:GlycerAld_3-P_DH_NAD(P)-bd	InterPro:GlycerAld_3-P_DH_cat	InterPro:Glyceraldehyde-3-P_DH_1	EnsemblPlants:KRH31426	ProteinID:KRH31426
ProteinID:KRH31426.1	InterPro:NAD(P)-bd_dom_sf	PFAM:PF00044	PFAM:PF02800	PIRSF:PIRSF000149	PRINTS:PR00078
ScanProsite:PS00071	PANTHER:PTHR10836	PANTHER:PTHR10836:SF63	MetaCyc:PWY-1042	MetaCyc:PWY-5484	MetaCyc:PWY-6901
MetaCyc:PWY-7003	SMART:SM00846	SUPFAM:SSF51735	SUPFAM:SSF55347	TIGRFAMs:TIGR01534	UniParc:UPI0001B16537
SEG:seg	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr11:+:34032568..34035339

Molecular Weight (calculated)

37016.3 Da

IEP (calculated)

7.611

GRAVY (calculated)

-0.106

Length

340 amino acids

Sequence
(BLAST)

001: MASDKKIRIG INGFGRIGRL VARVALQRND VELVAVNDPF ITTDYMTYMF KYDTVHGQWK HFDVKLKDSK TLLFGEKPVT VFGFRNPEEI PWGEVGADYV
101: VESTGVFTDK DKAAAHLKGG AKKVVISAPS KDAPMFVVGV NEKEYKPELD IVSNASCTTN CLAPLAKVIN DRFGIVEGLM TTVHAITATQ KTVDGPSNKD
201: WRGGRAASFN IIPSSTGAAK AVGKVLPALN GKLTGMSFRV PTVDVSVVDL TVRLEKPATY EQIKAAIKEE SEGKLKGILG YTEDDVVSTD FVGDNRSSIF
301: DAKAGISLNE NFVKLVSWYD NEWGYSSRVI DLIAHIASVA

Best Arabidopsis Sequence Match ( AT3G04120.1 )
(BLAST)

001: MADKKIRIGI NGFGRIGRLV ARVVLQRDDV ELVAVNDPFI TTEYMTYMFK YDSVHGQWKH NELKIKDEKT LLFGEKPVTV FGIRNPEDIP WAEAGADYVV
101: ESTGVFTDKD KAAAHLKGGA KKVVISAPSK DAPMFVVGVN EHEYKSDLDI VSNASCTTNC LAPLAKVIND RFGIVEGLMT TVHSITATQK TVDGPSMKDW
201: RGGRAASFNI IPSSTGAAKA VGKVLPALNG KLTGMSFRVP TVDVSVVDLT VRLEKAATYD EIKKAIKEES EGKLKGILGY TEDDVVSTDF VGDNRSSIFD
301: AKAGIALSDK FVKLVSWYDN EWGYSSRVVD LIVHMSKA

Arabidopsis Description

GAPC1Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic [Source:UniProtKB/Swiss-Prot;Acc:P25858]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.