CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, nucleus

Predictor Summary:

cytosol 4
plastid 1
mitochondrion 4

Predictors

GFP

MS/MS

Papers

cytosol: 18757068
endoplasmic reticulum: 21107622
nucleus: 21132161
extracellular: 22945350
endoplasmic reticulum: 23041469
nucleus: 24385303
endoplasmic reticulum: 27224218
nucleus: 27291164
endoplasmic reticulum: 28499913
mitochondrion: 28499913
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 22945350 doi

C Liao, F Hochholdinger, C Li

Key Laboratory of Plant-Soil Interactions, Ministry of Education, Center for Resources, Environment and Food Security, China Agricultural University, Beijing, China.

msms PMID: 21107622 doi

N Ahsan, T Nakamura, S Komatsu

National Institute of Crop Science, 2-1-18 Kannondai, Tsukuba, 305-8518, Japan.

msms PMID: 18757068 doi

NW Oehrle, AD Sarma, JK Waters, DW Emerich

United States Department of Agriculture, Curtis Hall, University of Missouri, Columbia, MO 65211, United States.

msms PMID: 23041469 doi

S Komatsu, R Kuji, Y Nanjo, S Hiraga, K Furukawa

National Institute of Crop Science, Tsukuba 305-8518, Japan. skomatsu@affrc.go.jp

msms PMID: 24385303 doi

S Komatsu, S Hiraga, MZ Nouri

National Institute of Crop Science, National Agriculture and Food Research Organization, Kannondai 2-1-18, Tsukuba, 305-8518, Japan, skomatsu@affrc.go.jp.

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27291164 doi

X Yin, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 27224218 doi

X Wang, S Komatsu

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH16972	cytosol, cytosol, mitochondrion
KRH72603	cytosol, cytosol, mitochondrion

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G13440.1	KRH16972	AT2G36460.1	23316205
AT1G13440.1	KRH72603	AT2G36460.1	23316205
AT1G13440.1	KRH16972	AT2G36460.1	23316205
AT1G13440.1	KRH72603	AT2G36460.1	23316205
AT3G04120.1	KRH16972	AT2G36460.1	23316205
AT3G04120.1	KRH72603	AT2G36460.1	23316205
AT3G04120.1	KRH16972	AT2G36460.1	23316205
AT3G04120.1	KRH72603	AT2G36460.1	23316205

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH63724	Soybean	nucleus	99.11	99.11
KRH63725	Soybean	endoplasmic reticulum, nucleus	98.82	98.82
KRH54234	Soybean	endoplasmic reticulum, nucleus	98.22	98.22
CDY49358	Canola	cytosol, mitochondrion	88.46	88.99
VIT_01s0010g02460.t01	Wine grape	cytosol	89.35	88.82
Bra040213.1-P	Field mustard	cytosol	88.46	88.46
KRG97466	Soybean	endoplasmic reticulum, nucleus	88.76	88.24
Os04t0486600-01	Rice	plastid	87.57	87.83
KRH31426	Soybean	endoplasmic reticulum	88.17	87.65
AT3G04120.1	Thale cress	cytosol	87.57	87.57
AT1G13440.1	Thale cress	cytosol	87.57	87.57
Bra040146.1-P	Field mustard	cytosol	87.57	87.57
Bra016729.1-P	Field mustard	cytosol	87.28	87.02
Bra026904.1-P	Field mustard	cytosol	86.98	86.98
Bra019722.1-P	Field mustard	cytosol	74.85	86.05
CDX92059	Canola	cytosol, endoplasmic reticulum, peroxisome	88.17	85.63
Os02t0601300-01	Rice	plasma membrane	84.91	85.16
EES05377	Sorghum	cytosol	84.62	84.87
KXG21112	Sorghum	cytosol	84.32	84.57
CDY42906	Canola	cytosol	88.17	84.42
CDX74199	Canola	endoplasmic reticulum	87.87	84.38
Zm00001d049641_P001	Maize	plasma membrane, plastid	84.02	84.27
TraesCS7D01G309500.1	Wheat	cytosol	84.02	84.27
TraesCS7B01G213300.1	Wheat	golgi	84.02	84.27
KXG20851	Sorghum	cytosol	83.73	83.98
Zm00001d051001_P002	Maize	cytosol	83.73	83.98
TraesCS6A01G213700.1	Wheat	unclear	83.43	83.68
TraesCS6D01G196300.2	Wheat	golgi, nucleus	83.43	83.68
KXG24320	Sorghum	cytosol	83.43	83.68
KRH57621	Soybean	endoplasmic reticulum	83.14	83.38
KRG94350	Soybean	cytosol, mitochondrion	82.84	83.09
Os08t0126300-01	Rice	cytosol	82.54	82.79
OQU77090	Sorghum	cytosol	54.14	82.43
TraesCS7A01G313100.1	Wheat	cytosol	82.25	80.58
Zm00001d035156_P003	Maize	cytosol	82.84	80.23
CDX98100	Canola	cytosol, mitochondrion, peroxisome	74.85	79.56
CDY50169	Canola	cytosol	87.28	79.3
CDY21098	Canola	cytosol	87.28	79.09
TraesCS7B01G234400.1	Wheat	cytosol	80.18	79.01
TraesCS6B01G243700.1	Wheat	endoplasmic reticulum	83.43	78.77
HORVU6Hr1G054520.3	Barley	cytosol, mitochondrion	83.43	77.47
CDY26651	Canola	mitochondrion, peroxisome	74.85	75.98
CDY21135	Canola	cytosol, mitochondrion, peroxisome, plastid	76.33	75.66
CDY30032	Canola	cytosol	87.28	75.06
HORVU7Hr1G074690.3	Barley	extracellular, plasma membrane	84.02	72.26
CDY29987	Canola	cytosol, peroxisome, plastid	76.33	68.62
CDY16090	Canola	cytoskeleton, cytosol, peroxisome	86.69	68.14
KRH66238	Soybean	plastid	71.01	57.42
KRH07324	Soybean	extracellular, nucleus	70.12	56.7
Zm00001d017121_P001	Maize	plastid	83.73	55.49
KRH37937	Soybean	plastid	12.43	47.73
KRH08444	Soybean	cytosol	31.95	41.38
KRG94751	Soybean	extracellular, nucleus	47.04	39.45
KRH06774	Soybean	nucleus	46.75	39.21
KRH74912	Soybean	cytosol	15.68	37.86
KRH60895	Soybean	nucleus, plastid	49.11	36.81
KRH51576	Soybean	nucleus, plastid	49.11	36.64
KRH70104	Soybean	plastid	26.92	32.97
KRH26225	Soybean	mitochondrion	27.81	29.65

Protein Annotations

KEGG:00010+1.2.1.12	KEGG:00710+1.2.1.12	EntrezGene:100037457	MapMan:2.1.1.4.1	Gene3D:3.30.360.10	Gene3D:3.40.50.720
EMBL:ACUP02003509	EMBL:DQ355800	EnsemblPlantsGene:GLYMA_06G172600	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824
GO:GO:0005488	GO:GO:0005975	GO:GO:0006006	GO:GO:0008150	GO:GO:0008152	GO:GO:0016491
GO:GO:0016620	GO:GO:0050661	GO:GO:0051287	GO:GO:0055114	InterPro:GlycerAld/Erythrose_P_DH	InterPro:GlycerAld_3-P_DH_AS
InterPro:GlycerAld_3-P_DH_NAD(P)-bd	InterPro:GlycerAld_3-P_DH_cat	InterPro:Glyceraldehyde-3-P_DH_1	EnsemblPlants:KRH54227	ProteinID:KRH54227	ProteinID:KRH54227.1
InterPro:NAD(P)-bd_dom_sf	PFAM:PF00044	PFAM:PF02800	PIRSF:PIRSF000149	PRINTS:PR00078	ScanProsite:PS00071
PANTHER:PTHR10836	PANTHER:PTHR10836:SF62	MetaCyc:PWY-1042	MetaCyc:PWY-5484	MetaCyc:PWY-6901	MetaCyc:PWY-7003
UniProt:Q2I0H4	SMART:SM00846	SUPFAM:SSF51735	SUPFAM:SSF55347	TIGRFAMs:TIGR01534	UniParc:UPI000068F503
SEG:seg	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr6:-:14465843..14468881

Molecular Weight (calculated)

36766.1 Da

IEP (calculated)

7.273

GRAVY (calculated)

-0.102

Length

338 amino acids

Sequence
(BLAST)

001: MGKVKIGING FGRIGRLVAR VALQRDDVEL VAVNDPFITT DYMTYMFKYD SVHGHWKHHD VTVKDEKTLL FGDKPVTIFG HRNPEEIPWG STGADIIVES
101: TGVFTDKDKA AAHLKGGAKK VIISAPSKDA PMFVVGVNEH EYKPELDIIS NASCTTNCLA PLAKVINDRF GIVEGLMTTV HSITATQKTV DGPSAKDWRG
201: GRAASFNIIP SSTGAAKAVG KVLPALNGKL TGMAFRVPTV DVSVVDLTVR LEKEASYDEI KNAIKEESEG KLKGILGYTE DDVVSTDFIG DSRSSIFDAK
301: AGIALNKNFV KLVSWYDNEW GYSSRVIDLL VFVAKKSL

Best Arabidopsis Sequence Match ( AT1G13440.1 )
(BLAST)

001: MADKKIRIGI NGFGRIGRLV ARVVLQRDDV ELVAVNDPFI TTEYMTYMFK YDSVHGQWKH HELKVKDDKT LLFGEKPVTV FGIRNPEDIP WGEAGADFVV
101: ESTGVFTDKD KAAAHLKGGA KKVVISAPSK DAPMFVVGVN EHEYKSDLDI VSNASCTTNC LAPLAKVIND RFGIVEGLMT TVHSITATQK TVDGPSMKDW
201: RGGRAASFNI IPSSTGAAKA VGKVLPSLNG KLTGMSFRVP TVDVSVVDLT VRLEKAATYD EIKKAIKEES EGKMKGILGY TEDDVVSTDF VGDNRSSIFD
301: AKAGIALSDK FVKLVSWYDN EWGYSSRVVD LIVHMSKA

Arabidopsis Description

GAPC2Glyceraldehyde-3-phosphate dehydrogenase GAPC2, cytosolic [Source:UniProtKB/Swiss-Prot;Acc:Q9FX54]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.