CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 5
cytosol 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, mitochondrion, nucleus
BaCelLo:	nucleus
EpiLoc:	cytosol
MultiLoc:	nucleus
Plant-mPloc:	nucleus
PProwler:	mitochondrion
WoLF PSORT:	nucleus
YLoc:	nucleus

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG99418
KRH02410
KRH05348
KRH05606
KRH06174
KRH15434
KRH15679
KRH19914
KRH24348
KRH30849
KRH32528
KRH32724
KRH39697
KRH47589
KRH50675
KRH52077
KRH52333
KRH61389
KRH61671
KRH62676
KRH73515

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G25150.1	KRH06174	AT1G17440.1	17340043
AT5G25150.1	KRH47589	AT1G17440.1	17340043
AT5G25150.1	KRH05606	AT1G27720.1	17340043
AT5G25150.1	KRH15434	AT1G27720.1	17340043
AT5G25150.1	KRH52077	AT1G27720.1	17340043
AT5G25150.1	KRH61389	AT1G27720.1	17340043
AT5G25150.1	KRH39697	AT1G32750.1	17340043
AT5G25150.1	KRH62676	AT1G32750.1	17340043
AT5G25150.1	KRH02410	AT1G54140.1	17340043
AT5G25150.1	KRH50675	AT1G54140.1	17340043
AT5G25150.1	KRH73515	AT1G54140.1	17340043
AT5G25150.1	KRH30849	AT3G10070.1	17340043
AT5G25150.1	KRH32724	AT3G10070.1	17340043
AT5G25150.1	KRH19914	AT4G34340.1	17340043
AT5G25150.1	KRH32528	AT4G34340.1	17340043
AT5G25150.1	KRG99418	AT5G25150.1	17340043
AT5G25150.1	KRH05348	AT5G25150.1	17340043
AT5G25150.1	KRH15679	AT5G25150.1	17340043
AT5G25150.1	KRH52333	AT5G25150.1	17340043
AT5G25150.1	KRH61671	AT5G25150.1	17340043
AT5G25150.1	KRH24348	AT5G45600.1	17340043

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH61671	Soybean	nucleus	97.44	96.56
GSMUA_Achr9P22400_001	Banana	cytosol	21.57	83.63
Zm00001d044810_P001	Maize	nucleus	16.14	82.95
KRG99418	Soybean	cytosol, extracellular, plastid	10.86	82.76
VIT_04s0008g05780.t01	Wine grape	nucleus	81.3	79.73
KRH05348	Soybean	nucleus	77.38	77.96
HORVU0Hr1G022460.1	Barley	nucleus	54.3	73.62
Zm00001d053142_P001	Maize	nucleus	17.8	73.29
AT5G25150.1	Thale cress	nucleus	73.91	73.24
TraesCS7B01G416400.1	Wheat	nucleus	54.0	73.21
Bra036534.1-P	Field mustard	nucleus	73.45	72.9
CDY19814	Canola	nucleus	73.3	72.75
CDX87098	Canola	nucleus	73.3	72.75
KXG20501	Sorghum	nucleus	71.95	72.6
Solyc06g065700.2.1	Tomato	nucleus	74.81	72.41
Os06t0649500-01	Rice	nucleus	70.89	71.87
Zm00001d046810_P002	Maize	nucleus	72.25	71.17
Bra020485.1-P	Field mustard	nucleus	71.49	71.17
CDY05473	Canola	nucleus	71.49	71.17
CDY05378	Canola	nucleus	71.04	70.93
TraesCS7A01G508300.2	Wheat	nucleus	69.83	70.58
TraesCS5D01G324200.2	Wheat	nucleus	69.68	70.43
TraesCS5A01G317800.1	Wheat	nucleus	69.68	70.43
HORVU7Hr1G113760.2	Barley	nucleus	69.53	70.27
TraesCS5B01G318400.1	Wheat	nucleus	69.53	70.27
TraesCS7D01G496500.1	Wheat	nucleus	68.48	69.74
Zm00001d025112_P001	Maize	cytosol, nucleus, plastid	19.0	68.85
Zm00001d016330_P001	Maize	extracellular	14.18	64.83
GSMUA_Achr9P22410_001	Banana	cytosol	35.44	63.0
GSMUA_Achr7P26980_001	Banana	nucleus	71.49	62.04
KRH15679	Soybean	cytosol	10.26	53.54
KRH19247	Soybean	cytosol, endoplasmic reticulum	12.22	25.23
KRH02663	Soybean	cytosol, nucleus	12.07	24.92
KRH04500	Soybean	cytosol	8.75	20.71
KRH67917	Soybean	cytosol	10.26	19.83
KRH72083	Soybean	nucleus	15.99	18.6
KRH31616	Soybean	nucleus	15.99	18.6
KRH04498	Soybean	mitochondrion	7.09	18.01
KRH28415	Soybean	nucleus	14.93	17.74
KRG88454	Soybean	cytosol, mitochondrion, peroxisome, plastid	11.76	17.41
KRG94333	Soybean	cytosol	5.28	17.33
KRH61363	Soybean	nucleus	13.88	16.2
KRH33352	Soybean	plastid	6.18	16.14
KRH58014	Soybean	nucleus	14.78	15.71
KRH52050	Soybean	cytosol, nucleus, peroxisome	14.18	14.71
KRH03286	Soybean	nucleus	18.1	13.65
KRH57076	Soybean	plasma membrane	16.29	12.77
KRH67774	Soybean	nucleus, plastid	17.19	12.09
KRG96051	Soybean	nucleus	17.04	11.97
KRH04503	Soybean	nucleus	5.88	11.68

Protein Annotations

Gene3D:1.25.40.500	EntrezGene:100781563	MapMan:15.3.4.3.1	MapMan:15.3.5.3.5	Gene3D:2.130.10.10	EMBL:ACUP02003419
InterPro:G-protein_beta_WD-40_rep	EnsemblPlantsGene:GLYMA_06G062300	GO:GO:0003674	GO:GO:0005488	GO:GO:0005515	UniProt:I1K8N0
InterPro:IPR001680	InterPro:IPR006594	InterPro:IPR015943	InterPro:IPR017986	InterPro:IPR037264	EnsemblPlants:KRH52333
ProteinID:KRH52333	ProteinID:KRH52333.1	InterPro:LisH	PFAM:PF00400	PFAM:PF04494	PRINTS:PR00320
ScanProsite:PS00678	PFscan:PS50082	PFscan:PS50294	PFscan:PS50896	PANTHER:PTHR19879	SMART:SM00320
SUPFAM:SSF160897	SUPFAM:SSF50978	InterPro:TFIID_NTD2	InterPro:TFIID_NTD2_sf	UniParc:UPI0002339590	InterPro:WD40/YVTN_repeat-like_dom_sf
InterPro:WD40_repeat	InterPro:WD40_repeat_CS	InterPro:WD40_repeat_dom	InterPro:WD40_repeat_dom_sf	SEG:seg	:

Description

hypothetical protein

Coordinates

chr6:+:4691667..4698974

Molecular Weight (calculated)

73332.6 Da

IEP (calculated)

7.046

GRAVY (calculated)

-0.389

Length

663 amino acids

Sequence
(BLAST)

001: MEDEKIVGYV TAFLKKKGFT QTEKIFQEEF QHNKSNTSNS VLEPDIANHL LAFSQLETGP ARFHDGYSRL RTWTYSSLDL YKHELLRVLY PVFIHCFMDL
101: VAKGHVQEAR NFFNTFREDH EMMHLRDLQK LEGVLSPNHL KEMEFAHSLR KSKFNIKICE YSYELLLQHL HSTQSTTILG IINEHINFQV TPGQPSLISD
201: DPEAVTLTGS SQEAANQINQ KEIHWGLLED SLEERLEKAG ALLSDSEKGE GEAKEGENDE TKKRSIEGGK QGASVKKVKK DKGGSATGKS AKPEANTIPS
301: APRVKPELPL PVIPAEAEQS VLEDLRNRVQ LSSVALPSVS FYTFINTHNG LSCSSISHDG SLIAGGFSDS SLKVWDMAKL GQQQTSSLSQ GENEQIFGQG
401: GGKRQYTLFQ GHSGPVYAAS FSPVGDFILS SSADSTIRLW STKLNANLVC YKGHNYPVWD VQFSPVGHYF ASSSHDRTAR IWSMDRIQPL RIMAGHLSDV
501: DCVQWHANCN YIATGSSDKT VRLWDVQSGE CVRVFVGHRG MILSLAMSPD GRYMASGDED GTIMMWDLSS GRCLTPLIGH TSCVWSLAFS SEGSVIASGS
601: ADCTVKLWDV NTSTKVSRAE EKGGSANRLR SLKTLPTKST PVYSLRFSRR NLLFAAGALS KSG

Best Arabidopsis Sequence Match ( AT5G25150.1 )
(BLAST)

001: MDPEQINEFV VGYLKKKGFS SAAKDLESYH HQNNNGSSFT SVDYHNDPEL TKLIRSFSQQ EDDPTRYREG YSKLRSWAYN SLDLYKHELL RVMYPVFIHC
101: YMDLVGKGHT QEARAFFNSF RKDHEMVHLR DLQKLEGVLS PSHLEEMEFA RSLRKSKVNI KFCQYSYELL LQYLHSTVST LMLGIINEHI NFQVYSGQPT
201: SSSDDIEAVT IVGSFQDTAN HINQKEIQWG LLEDSLEDRL EKTGGLLSDS EKGQGESKDG DADDSKKRST EIGKQGSSLK KLKKDKAGNA TAKVARLETI
301: TVSPAPRVKP ELALPVMSTD VEQSILEDLR NRVQLSSVAM PSVSFYTFVN THNGLNCSSI SHDGSLVAGG FSDSSIKVWD MAKIGQAGSG ALQAENDSSD
401: QSIGPNGRRS YTLLLGHSGP VYSATFSPPG DFVLSSSADT TIRLWSTKLN ANLVCYKGHN YPVWDAQFSP FGHYFASCSH DRTARIWSMD RIQPLRIMAG
501: HLSDVDCVQW HPNCNYIATG SSDKTVRLWD VQTGECVRIF IGHRSMVLSL AMSPDGRYMA SGDEDGTIMM WDLSTARCIT PLMGHNSCVW SLSYSGEGSL
601: LASGSADCTV KLWDVTSSTK LTKAEEKNGN SNRLRSLRTF PTKSTPVHAL RFSRRNLLFA AGAISKPAN

Arabidopsis Description

TAF5Transcription initiation factor TFIID subunit 5 [Source:UniProtKB/Swiss-Prot;Acc:Q6S7B0]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.