CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: mitochondrion, endoplasmic reticulum, nucleus

Predictor Summary:

cytosol 5
mitochondrion 1
cytoskeleton 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
mitochondrion: 25806999
endoplasmic reticulum: 27224218

msms PMID: 25806999 doi

AH Kamal, S Komatsu

National Institute of Crop Science, National Agriculture and Food Research Organization, Kannondai 2-1-18, Tsukuba 305-8518, Japan.

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG93168
KRG95386
KRH01660
KRH04597
KRH10268
KRH23061
KRH24799
KRH28456
KRH29804
KRH38098
KRH56507
KRH57934
KRH63322
KRH67068
KRH70465
KRH71847
KRH77015

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G37620.1	KRH04597	AT2G23420.1	21798944
AT2G37620.1	KRH28456	AT2G23420.1	21798944
AT2G37620.1	KRH57934	AT2G23420.1	21798944
AT2G37620.1	KRH63322	AT2G23420.1	21798944
AT2G37620.1	KRH77015	AT2G23420.1	21798944
AT2G37620.1	KRG93168	AT2G37620.1	21798944
AT2G37620.1	KRG95386	AT2G37620.1	21798944
AT2G37620.1	KRH01660	AT2G37620.1	21798944
AT2G37620.1	KRH10268	AT2G37620.1	21798944
AT2G37620.1	KRH23061	AT2G37620.1	21798944
AT2G37620.1	KRH24799	AT2G37620.1	21798944
AT2G37620.1	KRH29804	AT2G37620.1	21798944
AT2G37620.1	KRH38098	AT2G37620.1	21798944
AT2G37620.1	KRH56507	AT2G37620.1	21798944
AT2G37620.1	KRH67068	AT2G37620.1	21798944
AT2G37620.1	KRH70465	AT2G37620.1	21798944
AT2G37620.1	KRH71847	AT2G37620.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRG95386	Soybean	cytosol	99.47	99.47
KRH38098	Soybean	cytosol	98.67	98.67
KRH71847	Soybean	endoplasmic reticulum, nucleus	98.41	98.41
KRH10268	Soybean	mitochondrion, nucleus	98.14	98.14
KRH23061	Soybean	endoplasmic reticulum, mitochondrion, nucleus	98.14	98.14
VIT_08s0007g06520.t01	Wine grape	cytosol	97.61	97.61
KRH29804	Soybean	endoplasmic reticulum, mitochondrion, nucleus	97.61	97.61
KRH43389	Soybean	cytosol	97.08	97.08
GSMUA_AchrUn_...	Banana	plasma membrane	97.08	97.08
KRH01660	Soybean	mitochondrion, nucleus	97.08	97.08
KRH56507	Soybean	endoplasmic reticulum, mitochondrion	97.08	97.08
KRH59535	Soybean	cytosol	97.08	97.08
GSMUA_Achr10P...	Banana	cytosol	67.64	96.96
KRH64098	Soybean	cytosol	96.82	96.82
KRH70465	Soybean	mitochondrion, nucleus	96.82	96.82
PGSC0003DMT400060953	Potato	cytosol	96.82	96.82
KRG93168	Soybean	mitochondrion, nucleus	96.82	96.82
Solyc10g080500.1.1	Tomato	extracellular, nucleus, plastid, unclear	96.82	96.82
AT3G12110.1	Thale cress	cytosol	96.55	96.55
AT3G46520.2	Thale cress	cytosol	96.55	96.55
KRH53856	Soybean	endoplasmic reticulum, mitochondrion	84.88	96.39
CDX71119	Canola	cytosol	96.29	96.29
Bra019486.1-P	Field mustard	cytosol	96.29	96.29
CDY38066	Canola	cytosol	96.29	96.29
Bra018210.1-P	Field mustard	cytosol	96.29	96.29
CDY21777	Canola	cytosol	96.29	96.29
CDX99111	Canola	cytosol	96.29	96.29
AT5G59370.2	Thale cress	cytosol	96.29	96.29
CDX88698	Canola	cytosol	96.29	96.29
CDX86081	Canola	cytosol	96.29	96.29
Bra006719.1-P	Field mustard	cytosol	84.62	96.08
CDX82985	Canola	cytosol	96.02	96.02
AT3G53750.2	Thale cress	cytosol	96.02	96.02
Bra007025.1-P	Field mustard	cytosol	96.02	96.02
CDX78202	Canola	cytosol	96.02	96.02
AT2G37620.1	Thale cress	cytosol	96.02	96.02
Bra033819.1-P	Field mustard	cytosol	96.02	96.02
Bra000010.1-P	Field mustard	cytosol	95.76	95.76
CDY01102	Canola	cytosol	95.76	95.76
Bra014865.1-P	Field mustard	cytosol	95.76	95.76
CDX76191	Canola	cytosol	95.76	95.76
CDY53444	Canola	cytosol	95.76	95.76
Bra034778.1-P	Field mustard	cytosol	95.76	95.76
CDX73527	Canola	cytosol	89.66	95.75
Bra020319.1-P	Field mustard	cytosol	86.21	95.59
CDY48688	Canola	cytosol	86.21	95.59
CDX74944	Canola	cytosol	95.49	95.49
CDX91373	Canola	cytosol	95.49	95.49
Bra017166.1-P	Field mustard	cytosol	95.49	95.49
CDY08455	Canola	cytosol	95.49	95.49
CDY30846	Canola	cytosol	95.23	95.23
CDY07779	Canola	cytosol	88.86	95.17
CDY65255	Canola	cytosol	83.55	95.17
CDX93360	Canola	cytosol	83.55	95.17
Bra005178.1-P	Field mustard	cytosol	88.86	94.63
KRH43946	Soybean	endoplasmic reticulum, mitochondrion, nucleus	93.9	93.9
KRH10484	Soybean	endoplasmic reticulum, mitochondrion, nucleus	93.9	93.9
GSMUA_Achr7P18630_001	Banana	cytosol	78.78	92.81
CDX94448	Canola	cytosol	92.84	90.44
CDX79746	Canola	cytosol	88.86	90.05
Bra003156.1-P	Field mustard	cytosol	48.54	88.83
KRH24799	Soybean	cytosol	93.37	85.85
CDY71336	Canola	cytosol	54.91	75.27
CDX67573	Canola	cytosol	55.44	68.75
Bra002571.1-P	Field mustard	nucleus	96.02	59.83
CDY33033	Canola	nucleus	78.51	54.71
CDX80274	Canola	nucleus	77.19	53.01
KRG94584	Soybean	cytosol	44.3	42.93
KRH06905	Soybean	cytosol	43.77	42.42
KRH39934	Soybean	cytosol	32.89	37.58
KRH13968	Soybean	cytosol, nucleus	35.81	37.4
KRH32953	Soybean	cytosol	35.54	37.12
KRH23861	Soybean	cytosol, nucleus	35.54	37.12
KRH66446	Soybean	nucleus	42.97	36.4
KRH48882	Soybean	nucleus	42.44	35.87
KRH41617	Soybean	nucleus	40.58	35.5
KRH59222	Soybean	cytosol	17.77	34.9
KRH59228	Soybean	nucleus	37.93	33.41
KRH61849	Soybean	cytosol, nucleus	33.42	28.9

Protein Annotations

EntrezGene:100781142	MapMan:20.2.1	Gene3D:3.30.420.40	Gene3D:3.90.640.10	EMBL:ACUP02002025	InterPro:Actin
InterPro:Actin/actin-like_CS	InterPro:Actin_CS	EnsemblPlantsGene:GLYMA_03G144800	GO:GO:0000166	GO:GO:0003674	GO:GO:0005488
GO:GO:0005524	UniProt:I1JNK9	ProteinID:KRH67066.1	ProteinID:KRH67067.1	EnsemblPlants:KRH67068	ProteinID:KRH67068
ProteinID:KRH67068.1	ProteinID:KRH67069.1	ProteinID:KRH67070.1	PFAM:PF00022	PRINTS:PR00190	ScanProsite:PS00406
ScanProsite:PS00432	ScanProsite:PS01132	PANTHER:PTHR11937	PANTHER:PTHR11937:SF355	SMART:SM00268	SUPFAM:SSF53067
UniParc:UPI000233A1A3	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr3:-:36035905..36040284

Molecular Weight (calculated)

41655.1 Da

IEP (calculated)

5.162

GRAVY (calculated)

-0.176

Length

377 amino acids

Sequence
(BLAST)

001: MADAEDIQPL VCDNGTGMVK AGFAGDDAPR AVFPSIVGRP RHTGVMVGMG QKDAYVGDEA QSKRGILTLK YPIEHGIVSN WDDMEKIWHH TFYNELRVAP
101: EEHPVLLTEA PLNPKANREK MTQIMFETFN TPAMYVAIQA VLSLYASGRT TGIVLDSGDG VSHTVPIYEG YALPHAILRL DLAGRDLTDA LMKILTERGY
201: SFTTSAEREI VRDMKEKLAY IALDYEQELE TAKTSSAVEK SYELPDGQVI TIGAERFRCP EVLFQPSMIG MESPGIHETT YNSIMKCDVD IRKDLYGNIV
301: LSGGSTMFPG IADRMSKEIT ALAPGSMKIK VVAPPERKYS VWIGGSILAS LSTFQQMWIA KAEYDESGPS IVHRKCF

Best Arabidopsis Sequence Match ( AT3G12110.1 )
(BLAST)

001: MADGEDIQPL VCDNGTGMVK AGFAGDDAPR AVFPSIVGRP RHTGVMVGMG QKDAYVGDEA QSKRGILTLK YPIEHGIVSN WDDMEKIWHH TFYNELRVAP
101: EEHPVLLTEA PLNPKANREK MTQIMFETFN TPAMYVAIQA VLSLYASGRT TGIVLDSGDG VSHTVPIYEG YALPHAILRL DLAGRDLTDY LMKILTERGY
201: SFTTSAEREI VRDVKEKLAY IALDYEQEME TANTSSSVEK SYELPDGQVI TIGGERFRCP EVLFQPSLVG MEAAGIHETT YNSIMKCDVD IRKDLYGNIV
301: LSGGTTMFPG IADRMSKEIT ALAPSSMKIK VVAPPERKYS VWIGGSILAS LSTFQQMWIA KAEYDESGPS IVHRKCF

Arabidopsis Description

ACT11Actin-11 [Source:UniProtKB/Swiss-Prot;Acc:P53496]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.