Factsheet for Os04t0518100-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

cytosol 3
vacuole 1
peroxisome 1
mitochondrion 1
plastid 1

Predictors

GFP

MS/MS

Papers

plasma membrane: 27800704

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os02t0208700-00
Os02t0626100-01
Os02t0626400-01
Os02t0626600-00
Os02t0627100-01
Os04t0518100-01
Os04t0518400-01
Os05t0427400-00
Os06t0594400-02
Os08t0308300-00
Os12t0461300-00
Os12t0520200-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G37040.1	Os02t0626100-01	AT2G37040.1	21798944
AT2G37040.1	Os02t0626400-01	AT2G37040.1	21798944
AT2G37040.1	Os02t0626600-00	AT2G37040.1	21798944
AT2G37040.1	Os02t0627100-01	AT2G37040.1	21798944
AT2G37040.1	Os04t0518100-01	AT2G37040.1	21798944
AT2G37040.1	Os04t0518400-01	AT2G37040.1	21798944
AT2G37040.1	Os05t0427400-00	AT2G37040.1	21798944
AT2G37040.1	Os08t0308300-00	AT2G37040.1	21798944
AT2G37040.1	Os12t0461300-00	AT2G37040.1	21798944
AT2G37040.1	Os12t0520200-01	AT2G37040.1	21798944
AT2G37040.1	Os02t0626100-01	AT3G10340.1	21798944
AT2G37040.1	Os02t0626400-01	AT3G10340.1	21798944
AT2G37040.1	Os02t0626600-00	AT3G10340.1	21798944
AT2G37040.1	Os02t0627100-01	AT3G10340.1	21798944
AT2G37040.1	Os04t0518100-01	AT3G10340.1	21798944
AT2G37040.1	Os04t0518400-01	AT3G10340.1	21798944
AT2G37040.1	Os05t0427400-00	AT3G10340.1	21798944
AT2G37040.1	Os08t0308300-00	AT3G10340.1	21798944
AT2G37040.1	Os12t0461300-00	AT3G10340.1	21798944
AT2G37040.1	Os12t0520200-01	AT3G10340.1	21798944
AT3G10340.1	Os02t0208700-00	AT1G15670.1	21798944
AT3G10340.1	Os06t0594400-02	AT1G15670.1	21798944
AT3G53260.1	Os02t0626100-01	AT3G10340.1	21798944
AT3G53260.1	Os02t0626400-01	AT3G10340.1	21798944
AT3G53260.1	Os02t0626600-00	AT3G10340.1	21798944
AT3G53260.1	Os02t0627100-01	AT3G10340.1	21798944
AT3G53260.1	Os04t0518100-01	AT3G10340.1	21798944
AT3G53260.1	Os04t0518400-01	AT3G10340.1	21798944
AT3G53260.1	Os05t0427400-00	AT3G10340.1	21798944
AT3G53260.1	Os08t0308300-00	AT3G10340.1	21798944
AT3G53260.1	Os12t0461300-00	AT3G10340.1	21798944
AT3G53260.1	Os12t0520200-01	AT3G10340.1	21798944
AT3G53260.1	Os02t0626100-01	AT3G53260.1	21798944
AT3G53260.1	Os02t0626400-01	AT3G53260.1	21798944
AT3G53260.1	Os02t0626600-00	AT3G53260.1	21798944
AT3G53260.1	Os02t0627100-01	AT3G53260.1	21798944
AT3G53260.1	Os04t0518100-01	AT3G53260.1	21798944
AT3G53260.1	Os04t0518400-01	AT3G53260.1	21798944
AT3G53260.1	Os05t0427400-00	AT3G53260.1	21798944
AT3G53260.1	Os08t0308300-00	AT3G53260.1	21798944
AT3G53260.1	Os12t0461300-00	AT3G53260.1	21798944
AT3G53260.1	Os12t0520200-01	AT3G53260.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
EES12521	Sorghum	cytosol	89.67	90.18
Os02t0626100-01	Rice	extracellular, plasma membrane	87.13	87.87
Zm00001d003016_P001	Maize	plastid	89.82	80.48
CDY18464	Canola	peroxisome	45.83	76.6
Os02t0627100-01	Rice	plasma membrane	76.94	76.3
Os04t0518400-01	Rice	extracellular, plasma membrane	76.8	76.05
Os05t0427400-00	Rice	cytosol, peroxisome, plastid	76.8	75.84
PGSC0003DMT400080765	Potato	peroxisome	46.53	75.63
Solyc03g036480.1.1	Tomato	peroxisome	10.89	75.49
Os02t0626400-01	Rice	mitochondrion, plasma membrane	75.81	74.65
Solyc03g042560.1.1	Tomato	peroxisome	48.94	74.25
PGSC0003DMT400055489	Potato	mitochondrion	41.02	74.17
Solyc03g036470.1.1	Tomato	mitochondrion	23.62	72.93
Solyc10g086180.1.1	Tomato	extracellular	72.56	72.15
Solyc03g078280.1.1	Tomato	peroxisome	26.31	72.09
PGSC0003DMT400080548	Potato	cytosol, peroxisome, plastid	72.28	71.87
Os02t0626600-00	Rice	plasma membrane	72.56	71.75
KRH34854	Soybean	cytosol, endoplasmic reticulum, peroxisome	69.17	71.18
AT3G10340.1	Thale cress	peroxisome	71.15	71.15
CDX67566	Canola	cytosol	48.8	70.26
PGSC0003DMT400049886	Potato	nucleus, peroxisome, plastid	68.46	70.25
PGSC0003DMT400055488	Potato	cytosol, peroxisome, plastid	71.71	70.22
Bra003126.1-P	Field mustard	cytosol	70.16	70.16
AT3G53260.1	Thale cress	cytosol	71.0	70.01
CDX78161	Canola	cytosol	48.94	69.76
CDY00922	Canola	cytosol	69.59	69.69
Bra029831.1-P	Field mustard	cytosol	69.59	69.69
CDY08611	Canola	cytosol	69.59	69.69
PGSC0003DMT400055531	Potato	cytosol, peroxisome, plastid	70.86	69.68
Bra017210.1-P	Field mustard	cytosol, peroxisome, plastid	71.15	69.67
KRG91925	Soybean	cytosol, peroxisome, plastid	70.58	69.6
CDX73567	Canola	cytosol	48.8	69.56
Solyc09g007900.2.1	Tomato	cytosol, endoplasmic reticulum, peroxisome	70.86	69.49
Solyc09g007920.2.1	Tomato	extracellular, nucleus	70.72	69.35
Solyc03g071860.2.1	Tomato	endoplasmic reticulum	35.78	69.32
Bra006985.1-P	Field mustard	cytosol	69.17	69.26
AT2G37040.1	Thale cress	cytosol, peroxisome, plastid	71.0	69.24
Solyc09g007910.2.1	Tomato	extracellular, nucleus	70.3	68.93
Os12t0520200-01	Rice	plasma membrane	66.2	68.82
CDY66761	Canola	cytosol, peroxisome, plastid	50.49	68.79
CDX99621	Canola	mitochondrion	53.04	68.56
CDX76219	Canola	cytosol	50.21	68.01
Solyc09g007890.1.1	Tomato	plastid	68.6	67.45
Bra039777.1-P	Field mustard	peroxisome	47.24	65.49
AT5G04230.2	Thale cress	peroxisome	64.64	65.47
CDY15917	Canola	cytosol, peroxisome, plastid	64.07	64.9
Bra030322.1-P	Field mustard	cytosol, peroxisome, plastid	63.79	64.61
Solyc03g078270.1.1	Tomato	endoplasmic reticulum	13.15	64.14
Bra005221.1-P	Field mustard	peroxisome	33.24	63.34
CDX74991	Canola	peroxisome	32.96	61.32
CDX91331	Canola	peroxisome	32.96	61.32
PGSC0003DMT400055529	Potato	cytosol	14.0	59.28
CDY13306	Canola	peroxisome	17.54	59.05
CDY18463	Canola	peroxisome	21.07	56.65
VIT_11s0016g01640.t01	Wine grape	peroxisome	52.48	55.79
GSMUA_Achr9P15990_001	Banana	peroxisome	54.74	55.05
VIT_11s0016g01660.t01	Wine grape	peroxisome	12.87	52.3
Solyc10g011930.1.1	Tomato	cytosol	9.05	50.39
Solyc10g011920.1.1	Tomato	cytosol	8.77	48.82
Solyc03g071870.1.1	Tomato	peroxisome	11.74	48.26
CDY61769	Canola	peroxisome	17.26	48.22
Bra028793.1-P	Field mustard	peroxisome	19.94	47.96
VIT_11s0016g01520.t01	Wine grape	peroxisome	59.97	47.37
Os12t0461300-00	Rice	plastid	16.83	47.04
Os08t0308300-00	Rice	plastid	10.47	39.36
VIT_11s0016g01510.t01	Wine grape	peroxisome	12.59	25.07

Protein Annotations

KEGG:00360+4.3.1.24	KEGG:00940+4.3.1.24	Gene3D:1.10.274.20	Gene3D:1.10.275.10	Gene3D:1.20.200.10	MapMan:9.2.1.1.1
EntrezGene:9271676	EMBL:AK100346	InterPro:Aromatic_Lyase	ProteinID:BAF15238.1	ProteinID:BAS90102.1	ProteinID:CAE05454.3
ProteinID:CAE05619.1	InterPro:Fumarase/histidase_N	GO:GO:0003674	GO:GO:0003824	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0006559	GO:GO:0008150	GO:GO:0008152	GO:GO:0009056
GO:GO:0009058	GO:GO:0009698	GO:GO:0009800	GO:GO:0009987	GO:GO:0016829	GO:GO:0016841
GO:GO:0019748	GO:GO:0045548	InterPro:IPR023144	InterPro:IPR024083	InterPro:L-Aspartase-like	EnsemblPlantsGene:Os04g0518100
EnsemblPlants:Os04t0518100-01	PFAM:PF00221	ScanProsite:PS00488	PANTHER:PTHR10362	PANTHER:PTHR10362:SF33	InterPro:Phe/His_NH3-lyase_AS
InterPro:Phe_NH3-lyase	InterPro:Phe_NH3-lyase_shielding_dom_sf	UniProt:Q7X720	SUPFAM:SSF48557	TIGRFAMs:TIGR01226	UniParc:UPI00001A53EF
RefSeq:XP_015635507.1	SEG:seg	:	:	:	:

Description

Phenylalanine ammonia-lyase 02Phenylalanine ammonia-lyase. (Os04t0518100-01)

Coordinates

chr4:-:25906878..25910059

Molecular Weight (calculated)

76043.1 Da

IEP (calculated)

6.375

GRAVY (calculated)

-0.068

Length

707 amino acids

Sequence
(BLAST)

001: MASQTADATG FVASDPLSWG KAALEMTGSH LDEVKRMVAQ SREAVVKIEG SSLRVGQVAA VSAAKDASGV VVELDEEARP RVKASSEWIL NCIAHGGDIY
101: GVTTGFGGTS HRRTKDGQAL QVELLRHLNA GIFGNGSDGN SLPSEVSRAA MLVRINTLLQ GYSGIRFEIL EAITKLINTG VSPCLPLRGT ITASGDLVPL
201: SYIAGLITGR PNAQAVTVDG KKVDAAEAFK IAGIQGGFFR LEPKEGLAIV NGTSVGSALA AMVLYDANVL AVLSEVLSAV FCEVMNGKPE YTDHLTHKLK
301: HHPGSIEAAA IMEHILAGSA FMPHAQKVNE VDPLLKPKQD RYALRTSPQW LGPQIEVIRA ATKSIEREVN SVNDNPVIDV HRGKALHGGN FQGTPIGVSM
401: DNTRLAIANI GKLMFAQFSE LVNEFYNNGL TSNLAGSRNP SLDYGFKGTE IAMASYCSEL QFLANPVTNH VQSAEQHNQD VNSLGLVSAR KTAEAVDILK
501: LMSSTYLVAL CQAVDLRHLE ENLKSAVKNC VTTVAKKVLT TGPAGGLHSA RFSEKALLTA IDREAVYSYA DDPCSANYPL MTKIRAVLVE HALANGPAEK
601: DDGSSVFSKI TAFEEELREA LPREMEAARV AFETGTAPIT NRIKESRSFP LYRFVREELG CVYLTGEKLK SPGEECNKVF LAISERKLID PMLECLKEWN
701: GEPLPIC

Best Arabidopsis Sequence Match ( AT2G37040.1 )
(BLAST)

001: MEINGAHKSN GGGVDAMLCG GDIKTKNMVI NAEDPLNWGA AAEQMKGSHL DEVKRMVAEF RKPVVNLGGE TLTIGQVAAI STIGNSVKVE LSETARAGVN
101: ASSDWVMESM NKGTDSYGVT TGFGATSHRR TKNGVALQKE LIRFLNAGIF GSTKETSHTL PHSATRAAML VRINTLLQGF SGIRFEILEA ITSFLNNNIT
201: PSLPLRGTIT ASGDLVPLSY IAGLLTGRPN SKATGPNGEA LTAEEAFKLA GISSGFFDLQ PKEGLALVNG TAVGSGMASM VLFETNVLSV LAEILSAVFA
301: EVMSGKPEFT DHLTHRLKHH PGQIEAAAIM EHILDGSSYM KLAQKLHEMD PLQKPKQDRY ALRTSPQWLG PQIEVIRYAT KSIEREINSV NDNPLIDVSR
401: NKAIHGGNFQ GTPIGVSMDN TRLAIAAIGK LMFAQFSELV NDFYNNGLPS NLTASRNPSL DYGFKGAEIA MASYCSELQY LANPVTSHVQ SAEQHNQDVN
501: SLGLISSRKT SEAVDILKLM STTFLVAICQ AVDLRHLEEN LRQTVKNTVS QVAKKVLTTG VNGELHPSRF CEKDLLKVVD REQVYTYADD PCSATYPLIQ
601: KLRQVIVDHA LINGESEKNA VTSIFHKIGA FEEELKAVLP KEVEAARAAY DNGTSAIPNR IKECRSYPLY RFVREELGTE LLTGEKVTSP GEEFDKVFTA
701: ICEGKIIDPM MECLNEWNGA PIPIC

Arabidopsis Description

PAL1Phenylalanine ammonia-lyase 1 [Source:UniProtKB/Swiss-Prot;Acc:P35510]
SUBAcon: [peroxisome,plastid,cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.