CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

cytosol 5
vacuole 1
nucleus 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
extracellular: 25724908
endoplasmic reticulum: 27224218
nucleus: 27291164
mitochondrion: 27780359
mitochondrion: 28499913
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27780359 doi

G Mustafa, S Komatsu

Graduate School of Life and Environmental Science, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 25724908 doi

VC Clarke, PC Loughlin, A Gavrin, C Chen, EM Brear, DA Day, PM Smith

From the ‡University of Sydney, School of Biological Sciences, Sydney Australia;, From the ‡University of Sydney, School of Biological Sciences, Sydney Australia; penny.smith@sydney.edu.au., From the ‡University of Sydney, School of Biological Sciences, Sydney Australia; §Flinders University, School of Biological Sciences, Adelaide Australia.

msms PMID: 27291164 doi

X Yin, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 27224218 doi

X Wang, S Komatsu

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH00551
KRH40615

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G07920.1	KRH00551	AT3G54220.1	19228333
AT1G07920.1	KRH40615	AT3G54220.1	19228333
AT1G07930.1	KRH00551	AT3G54220.1	19228333
AT1G07930.1	KRH40615	AT3G54220.1	19228333

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH04781	Soybean	endoplasmic reticulum	99.55	99.55
CDY09214	Canola	cytosol	34.9	97.5
KRH07098	Soybean	cytosol, mitochondrion, nucleus	97.32	97.32
HORVU5Hr1G051280.1	Barley	cytosol	22.6	97.12
Os03t0177400-01	Rice	cytosol	97.09	97.09
Os03t0177500-01	Rice	cytosol	97.09	97.09
Os03t0177900-01	Rice	plasma membrane	97.09	97.09
Os03t0178000-02	Rice	cytosol	97.09	97.09
KRH34904	Soybean	nucleus	96.87	96.65
KRG93959	Soybean	nucleus	96.64	96.64
KRH58241	Soybean	nucleus	96.64	96.64
PGSC0003DMT400020968	Potato	cytosol	96.42	96.42
Solyc06g005060.2.1	Tomato	cytosol	96.42	96.21
Solyc06g009970.2.1	Tomato	extracellular, nucleus, plastid	96.42	96.21
Solyc11g069700.1.1	Tomato	nucleus	96.2	96.2
GSMUA_Achr2P19000_001	Banana	cytosol	16.55	96.1
PGSC0003DMT400053547	Potato	cytosol	96.2	95.98
PGSC0003DMT400053553	Potato	cytosol	96.2	95.98
PGSC0003DMT400059830	Potato	cytosol	96.2	95.98
PGSC0003DMT400059832	Potato	cytosol	96.2	95.98
PGSC0003DMT400050663	Potato	cytosol	95.97	95.97
Zm00001d046449_P003	Maize	cytosol	95.97	95.97
KXG20293	Sorghum	cytosol	95.75	95.75
KXG20294	Sorghum	cytosol	95.75	95.75
KXG20296	Sorghum	cytosol	95.75	95.75
KXG20299	Sorghum	cytosol	95.75	95.75
VIT_06s0004g03240.t01	Wine grape	cytosol	95.75	95.75
Zm00001d036904_P001	Maize	cytosol	95.75	95.75
Solyc06g009960.1.1	Tomato	cytosol	87.7	95.61
Zm00001d037905_P010	Maize	cytosol	96.42	95.57
Bra031601.1-P	Field mustard	cytosol	42.28	95.45
Zm00001d009868_P015	Maize	cytosol	96.2	95.34
Zm00001d009870_P008	Maize	cytosol	96.2	95.34
Zm00001d037873_P010	Maize	cytosol	96.2	95.34
HORVU4Hr1G056740.1	Barley	cytosol	95.3	95.3
TraesCS4B01G196900.1	Wheat	golgi	95.3	95.3
TraesCS5B01G418100.1	Wheat	cytosol	95.3	95.3
TraesCS5B01G418200.1	Wheat	cytosol	95.3	95.3
CDY06287	Canola	cytosol	48.32	95.15
Zm00001d037882_P001	Maize	cytosol	39.37	95.14
Zm00001d037875_P014	Maize	cytosol	95.97	95.12
Zm00001d037877_P014	Maize	cytosol	95.97	95.12
CDY67375	Canola	cytosol	59.06	94.96
TraesCS4A01G107600.1	Wheat	cytosol	94.85	94.85
TraesCS4A01G107700.1	Wheat	cytosol	94.85	94.85
TraesCS5A01G415400.1	Wheat	cytosol	94.85	94.85
TraesCS5A01G415600.1	Wheat	cytosol	94.85	94.85
AT1G07920.1	Thale cress	cytosol	95.08	94.65
AT1G07930.1	Thale cress	cytosol	95.08	94.65
AT1G07940.3	Thale cress	cytosol	95.08	94.65
AT5G60390.2	Thale cress	cytosol	95.08	94.65
Bra002483.1-P	Field mustard	cytosol	95.08	94.65
Bra018669.1-P	Field mustard	cytosol	95.08	94.65
Bra031602.1-P	Field mustard	cytosol	95.08	94.65
CDX88757	Canola	cytosol	84.79	94.51
Bra018242.1-P	Field mustard	cytosol	94.85	94.43
Bra020253.1-P	Field mustard	cytosol	94.85	94.43
CDY09211	Canola	cytosol	90.38	94.39
CDY10804	Canola	cytosol	90.38	94.39
CDY34479	Canola	cytosol	90.38	94.39
CDY61938	Canola	cytosol	90.38	94.39
HORVU4Hr1G056830.1	Barley	cytosol	61.74	94.2
Bra031605.1-P	Field mustard	cytosol	89.93	94.15
CDY22710	Canola	cytosol	89.93	94.15
CDY22711	Canola	cytosol	89.93	94.15
CDY06289	Canola	cytosol	53.91	94.14
Bra020251.1-P	Field mustard	cytosol	86.58	93.93
Bra006661.1-P	Field mustard	cytosol	94.18	93.76
OQU75582	Sorghum	cytosol	26.17	93.6
HORVU5Hr1G100200.3	Barley	mitochondrion	95.3	91.81
HORVU5Hr1G100700.3	Barley	plasma membrane	95.3	91.61
HORVU6Hr1G085320.3	Barley	cytosol	88.37	89.57
CDY06288	Canola	cytosol	23.04	89.57
CDY06290	Canola	cytosol	23.04	89.57
CDY67376	Canola	cytosol	23.04	89.57
CDY69678	Canola	cytosol	23.04	89.57
HORVU4Hr1G056820.1	Barley	plasma membrane	95.3	89.5
Zm00001d009887_P001	Maize	cytosol	28.86	88.36
TraesCS4B01G196800.2	Wheat	golgi	95.3	88.2
CDX70032	Canola	cytosol	67.79	88.08
CDX93604	Canola	cytosol	90.38	87.83
CDX95108	Canola	cytosol, extracellular	95.08	87.27
HORVU6Hr1G085370.1	Barley	cytosol	87.92	86.95
OQU75597	Sorghum	cytosol	33.56	85.71
VIT_06s0004g03220.t01	Wine grape	cytosol	95.75	82.63
VIT_19s0014g04110.t01	Wine grape	cytosol	21.92	80.99
Bra030707.1-P	Field mustard	cytosol	95.08	72.03
CDY58828	Canola	cytosol	89.93	70.77
TraesCS2D01G323300.1	Wheat	cytosol, mitochondrion	15.66	66.04
KRH41785	Soybean	cytosol, nucleus	35.35	31.35
KRH60492	Soybean	cytosol	35.35	31.29
KRH20931	Soybean	mitochondrion	28.19	28.0
KRG95471	Soybean	nucleus	27.96	27.65
KRH67153	Soybean	nucleus	27.96	27.53
KRH54307	Soybean	mitochondrion, plastid	28.64	26.72
KRH63638	Soybean	endoplasmic reticulum, nucleus, plastid	28.41	26.46
KRH57147	Soybean	plastid	28.19	26.3
KRH11356	Soybean	mitochondrion	24.16	26.15
KRH03857	Soybean	plastid	25.73	25.73
KRH33293	Soybean	plastid	11.63	22.13
KRH42938	Soybean	cytosol	34.9	19.82
KRH59057	Soybean	cytosol	35.12	19.8

Protein Annotations

MapMan:17.5.1.1	Gene3D:2.40.30.10	Gene3D:3.40.50.300	UniProt:A0A0R4J384	EMBL:ACUP02003066	InterPro:EFTu-like_2
EnsemblPlantsGene:GLYMA_05G089000	GO:GO:0000166	GO:GO:0003674	GO:GO:0003676	GO:GO:0003723	GO:GO:0003746
GO:GO:0003824	GO:GO:0003924	GO:GO:0005488	GO:GO:0005525	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0006412	GO:GO:0006414	GO:GO:0008135	GO:GO:0008150
GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0016787	GO:GO:0019538	InterPro:G_TR_CS
InterPro:IPR000795	EnsemblPlants:KRH57863	ProteinID:KRH57863	ProteinID:KRH57863.1	ProteinID:KRH57864.1	HAMAP:MF_00118_A
InterPro:P-loop_NTPase	PFAM:PF00009	PFAM:PF03143	PFAM:PF03144	PRINTS:PR00315	ScanProsite:PS00301
PFscan:PS51722	PANTHER:PTHR23115	PANTHER:PTHR23115:SF230	SUPFAM:SSF50447	SUPFAM:SSF50465	SUPFAM:SSF52540
InterPro:TF_GTP-bd_dom	TIGRFAMs:TIGR00483	InterPro:Transl_B-barrel_sf	InterPro:Transl_elong_EF1A/Init_IF2_C	InterPro:Transl_elong_EF1A_euk/arc	InterPro:Transl_elong_EFTu/EF1A_C
UniParc:UPI000189CF9A	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr5:+:19281223..19284314

Molecular Weight (calculated)

49358.9 Da

IEP (calculated)

9.559

GRAVY (calculated)

-0.334

Length

447 amino acids

Sequence
(BLAST)

001: MGKEKVHISI VVIGHVDSGK STTTGHLIYK LGGIDKRVIE RFEKEAAEMN KRSFKYAWVL DKLKAERERG ITIDIALWKF ETTKYYCTVI DAPGHRDFIK
101: NMITGTSQAD CAVLIIDSTT GGFEAGISKD GQTREHALLS FTLGVKQMIC CCNKMDATTP KYSKARYDEI VKEVSSYLKK VGYNPDKIPF VPISGFEGDN
201: MIERSTNLDW YKGPTLLDAL DQISEPKRPS DKPLRLPLQD VYKIGGIGTV PVGRVETGVL KPGMVVTFAP TGLTTEVKSV EMHHEALTEA LPGDNVGFNV
301: KNVAVKDLKR GYVASNSKDD PAKEAANFTA QVIIMNHPGQ IGNGYAPVLD CHTSHIAVKF AELMTKIDRR SGKELEKEPK FLKNGDAGFV KMIPTKPMVV
401: ETFSEYPPLG RFAVRDMRQT VAVGVIKNVE KKDPTGAKVT KAAQKKK

Best Arabidopsis Sequence Match ( AT1G07930.1 )
(BLAST)

001: MGKEKFHINI VVIGHVDSGK STTTGHLIYK LGGIDKRVIE RFEKEAAEMN KRSFKYAWVL DKLKAERERG ITIDIALWKF ETTKYYCTVI DAPGHRDFIK
101: NMITGTSQAD CAVLIIDSTT GGFEAGISKD GQTREHALLA FTLGVKQMIC CCNKMDATTP KYSKARYDEI IKEVSSYLKK VGYNPDKIPF VPISGFEGDN
201: MIERSTNLDW YKGPTLLEAL DQINEPKRPS DKPLRLPLQD VYKIGGIGTV PVGRVETGMI KPGMVVTFAP TGLTTEVKSV EMHHESLLEA LPGDNVGFNV
301: KNVAVKDLKR GYVASNSKDD PAKGAANFTS QVIIMNHPGQ IGNGYAPVLD CHTSHIAVKF SEILTKIDRR SGKEIEKEPK FLKNGDAGMV KMTPTKPMVV
401: ETFSEYPPLG RFAVRDMRQT VAVGVIKSVD KKDPTGAKVT KAAVKKGAK

Arabidopsis Description

A1Elongation factor 1-alpha 4 [Source:UniProtKB/Swiss-Prot;Acc:Q8GTY0]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.