CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

cytosol 5
vacuole 1
nucleus 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
nucleus: 27291164
mitochondrion: 27780359

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27780359 doi

G Mustafa, S Komatsu

Graduate School of Life and Environmental Science, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 27291164 doi

X Yin, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 27224218 doi

X Wang, S Komatsu

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH00551
KRH40615

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G07920.1	KRH00551	AT3G54220.1	19228333
AT1G07920.1	KRH40615	AT3G54220.1	19228333
AT1G07930.1	KRH00551	AT3G54220.1	19228333
AT1G07930.1	KRH40615	AT3G54220.1	19228333

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRG93959	Soybean	nucleus	99.55	99.55
CDY09214	Canola	cytosol	35.12	98.12
HORVU5Hr1G051280.1	Barley	cytosol	22.82	98.08
KRH07098	Soybean	cytosol, mitochondrion, nucleus	97.54	97.54
Os03t0177400-01	Rice	cytosol	97.54	97.54
Os03t0177500-01	Rice	cytosol	97.54	97.54
Os03t0177900-01	Rice	plasma membrane	97.54	97.54
Os03t0178000-02	Rice	cytosol	97.54	97.54
Zm00001d046449_P003	Maize	cytosol	97.54	97.54
GSMUA_Achr2P19000_001	Banana	cytosol	16.78	97.4
KXG20293	Sorghum	cytosol	97.32	97.32
KXG20294	Sorghum	cytosol	97.32	97.32
KXG20296	Sorghum	cytosol	97.32	97.32
KXG20299	Sorghum	cytosol	97.32	97.32
PGSC0003DMT400050663	Potato	cytosol	97.32	97.32
VIT_06s0004g03240.t01	Wine grape	cytosol	97.32	97.32
Zm00001d036904_P001	Maize	cytosol	97.32	97.32
KRH34904	Soybean	nucleus	97.32	97.1
Solyc11g069700.1.1	Tomato	nucleus	97.09	97.09
CDY06287	Canola	cytosol	49.22	96.92
Zm00001d009870_P008	Maize	cytosol	97.54	96.67
Zm00001d037873_P010	Maize	cytosol	97.54	96.67
KRH57863	Soybean	nucleus	96.64	96.64
Bra031601.1-P	Field mustard	cytosol	42.73	96.46
Zm00001d037875_P014	Maize	cytosol	97.32	96.45
Zm00001d037877_P014	Maize	cytosol	97.32	96.45
AT1G07920.1	Thale cress	cytosol	96.87	96.44
AT1G07930.1	Thale cress	cytosol	96.87	96.44
AT1G07940.3	Thale cress	cytosol	96.87	96.44
AT5G60390.2	Thale cress	cytosol	96.87	96.44
PGSC0003DMT400020968	Potato	cytosol	96.42	96.42
CDX88757	Canola	cytosol	86.35	96.26
Zm00001d009868_P015	Maize	cytosol	97.09	96.23
Zm00001d037905_P010	Maize	cytosol	97.09	96.23
Zm00001d037882_P001	Maize	cytosol	39.82	96.22
Bra002483.1-P	Field mustard	cytosol	96.64	96.21
Bra018669.1-P	Field mustard	cytosol	96.64	96.21
Bra031602.1-P	Field mustard	cytosol	96.64	96.21
Solyc06g005060.2.1	Tomato	cytosol	96.42	96.21
Solyc06g009970.2.1	Tomato	extracellular, nucleus, plastid	96.42	96.21
HORVU4Hr1G056740.1	Barley	cytosol	96.2	96.2
KRH04781	Soybean	endoplasmic reticulum	96.2	96.2
TraesCS4B01G196900.1	Wheat	golgi	96.2	96.2
TraesCS5B01G418100.1	Wheat	cytosol	96.2	96.2
TraesCS5B01G418200.1	Wheat	cytosol	96.2	96.2
CDY09211	Canola	cytosol	91.95	96.03
CDY10804	Canola	cytosol	91.95	96.03
CDY34479	Canola	cytosol	91.95	96.03
CDY61938	Canola	cytosol	91.95	96.03
Bra018242.1-P	Field mustard	cytosol	96.42	95.99
Bra020253.1-P	Field mustard	cytosol	96.42	95.99
PGSC0003DMT400053547	Potato	cytosol	96.2	95.98
PGSC0003DMT400053553	Potato	cytosol	96.2	95.98
PGSC0003DMT400059830	Potato	cytosol	96.2	95.98
PGSC0003DMT400059832	Potato	cytosol	96.2	95.98
Bra031605.1-P	Field mustard	cytosol	91.5	95.78
CDY22710	Canola	cytosol	91.5	95.78
CDY22711	Canola	cytosol	91.5	95.78
TraesCS4A01G107600.1	Wheat	cytosol	95.75	95.75
TraesCS4A01G107700.1	Wheat	cytosol	95.75	95.75
TraesCS5A01G415400.1	Wheat	cytosol	95.75	95.75
TraesCS5A01G415600.1	Wheat	cytosol	95.75	95.75
CDY67375	Canola	cytosol	59.51	95.68
Solyc06g009960.1.1	Tomato	cytosol	87.7	95.61
Bra020251.1-P	Field mustard	cytosol	87.92	95.39
Bra006661.1-P	Field mustard	cytosol	95.75	95.32
CDY06289	Canola	cytosol	54.59	95.31
HORVU4Hr1G056830.1	Barley	cytosol	61.74	94.2
CDY06288	Canola	cytosol	24.16	93.91
CDY06290	Canola	cytosol	24.16	93.91
CDY67376	Canola	cytosol	24.16	93.91
CDY69678	Canola	cytosol	24.16	93.91
OQU75582	Sorghum	cytosol	25.95	92.8
HORVU5Hr1G100200.3	Barley	mitochondrion	96.2	92.67
HORVU5Hr1G100700.3	Barley	plasma membrane	96.2	92.47
HORVU6Hr1G085320.3	Barley	cytosol	89.26	90.48
HORVU4Hr1G056820.1	Barley	plasma membrane	95.75	89.92
Zm00001d009887_P001	Maize	cytosol	29.31	89.73
CDX70032	Canola	cytosol	68.9	89.53
CDX93604	Canola	cytosol	91.95	89.35
TraesCS4B01G196800.2	Wheat	golgi	96.2	89.03
CDX95108	Canola	cytosol, extracellular	96.64	88.71
HORVU6Hr1G085370.1	Barley	cytosol	89.04	88.05
OQU75597	Sorghum	cytosol	33.33	85.14
VIT_06s0004g03220.t01	Wine grape	cytosol	97.32	83.98
VIT_19s0014g04110.t01	Wine grape	cytosol	22.15	81.82
Bra030707.1-P	Field mustard	cytosol	96.64	73.22
CDY58828	Canola	cytosol	91.5	72.01
TraesCS2D01G323300.1	Wheat	cytosol, mitochondrion	15.88	66.98
KRH41785	Soybean	cytosol, nucleus	36.47	32.34
KRH60492	Soybean	cytosol	36.24	32.08
KRH20931	Soybean	mitochondrion	27.96	27.78
KRG95471	Soybean	nucleus	27.96	27.65
KRH67153	Soybean	nucleus	27.96	27.53
KRH63638	Soybean	endoplasmic reticulum, nucleus, plastid	28.86	26.88
KRH54307	Soybean	mitochondrion, plastid	28.64	26.72
KRH57147	Soybean	plastid	28.64	26.72
KRH03857	Soybean	plastid	25.95	25.95
KRH11356	Soybean	mitochondrion	23.71	25.67
KRH33293	Soybean	plastid	11.63	22.13
KRH42938	Soybean	cytosol	35.12	19.95
KRH59057	Soybean	cytosol	35.35	19.92

Protein Annotations

MapMan:17.5.1.1	Gene3D:2.40.30.10	Gene3D:3.40.50.300	UniProt:A0A0R0JTV5	EMBL:ACUP02003241	InterPro:EFTu-like_2
EnsemblPlantsGene:GLYMA_05G114900	GO:GO:0000166	GO:GO:0003674	GO:GO:0003676	GO:GO:0003723	GO:GO:0003746
GO:GO:0003824	GO:GO:0003924	GO:GO:0005488	GO:GO:0005525	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0006412	GO:GO:0006414	GO:GO:0008135	GO:GO:0008150
GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0016787	GO:GO:0019538	InterPro:G_TR_CS
InterPro:IPR000795	EnsemblPlants:KRH58241	ProteinID:KRH58241	ProteinID:KRH58241.1	HAMAP:MF_00118_A	InterPro:P-loop_NTPase
PFAM:PF00009	PFAM:PF03143	PFAM:PF03144	PRINTS:PR00315	ScanProsite:PS00301	PFscan:PS51722
PANTHER:PTHR23115	PANTHER:PTHR23115:SF230	SUPFAM:SSF50447	SUPFAM:SSF50465	SUPFAM:SSF52540	InterPro:TF_GTP-bd_dom
TIGRFAMs:TIGR00483	InterPro:Transl_B-barrel_sf	InterPro:Transl_elong_EF1A/Init_IF2_C	InterPro:Transl_elong_EF1A_euk/arc	InterPro:Transl_elong_EFTu/EF1A_C	UniParc:UPI000189CF9C
SEG:seg	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr5:-:30481777..30484482

Molecular Weight (calculated)

49266.8 Da

IEP (calculated)

9.578

GRAVY (calculated)

-0.326

Length

447 amino acids

Sequence
(BLAST)

001: MGKEKVHINI VVIGHVDSGK STTTGHLIYK LGGIDKRVIE RFEKEAAEMN KRSFKYAWVL DKLKAERERG ITIDIALWKF ETTKYYCTVI DAPGHRDFIK
101: NMITGTSQAD CAVLIIDSTT GGFEAGISKD GQTREHALLA FTLGVKQMIC CCNKMDATTP KYSKARYDEI VKEVSSYLKK VGYNPDKIPF VPISGFEGDN
201: MIERSTNLDW YKGPTLLEAL DQINEPKRPS DKPLRLPLQD VYKIGGIGTV PVGRVETGVV KPGMVVTFGP TGLTTEVKSV EMHHEALTEA LPGDNVGFNV
301: KNVAVKDLKR GFVASNSKDD PAKEAANFTS QVIIMNHPGQ IGNGYAPVLD CHTSHIAVKF SEILTKIDRR SGKELEKEPK FLKNGDAGMV KMVPTKPMVV
401: ETFSEYPPLG RFAVRDMRQT VAVGVIKSVE KKDPTGAKVT KAAAKKK

Best Arabidopsis Sequence Match ( AT1G07930.1 )
(BLAST)

001: MGKEKFHINI VVIGHVDSGK STTTGHLIYK LGGIDKRVIE RFEKEAAEMN KRSFKYAWVL DKLKAERERG ITIDIALWKF ETTKYYCTVI DAPGHRDFIK
101: NMITGTSQAD CAVLIIDSTT GGFEAGISKD GQTREHALLA FTLGVKQMIC CCNKMDATTP KYSKARYDEI IKEVSSYLKK VGYNPDKIPF VPISGFEGDN
201: MIERSTNLDW YKGPTLLEAL DQINEPKRPS DKPLRLPLQD VYKIGGIGTV PVGRVETGMI KPGMVVTFAP TGLTTEVKSV EMHHESLLEA LPGDNVGFNV
301: KNVAVKDLKR GYVASNSKDD PAKGAANFTS QVIIMNHPGQ IGNGYAPVLD CHTSHIAVKF SEILTKIDRR SGKEIEKEPK FLKNGDAGMV KMTPTKPMVV
401: ETFSEYPPLG RFAVRDMRQT VAVGVIKSVD KKDPTGAKVT KAAVKKGAK

Arabidopsis Description

A1Elongation factor 1-alpha 4 [Source:UniProtKB/Swiss-Prot;Acc:Q8GTY0]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.