Factsheet for PGSC0003DMT400066392

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

cytosol 4
nucleus 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol
Any Predictor:	cytosol, mitochondrion, nucleus
BaCelLo:	cytosol
MultiLoc:	cytosol
Plant-mPloc:	nucleus
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

PPI

Inferred distinct locusB in Crop

locusB	locations
PGSC0003DMT400053153

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G48690.1	PGSC0003DMT400053153	AT5G10110.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Solyc01g108570.2.1	Tomato	cytosol, extracellular	91.05	91.94
PGSC0003DMT400066386	Potato	cytosol	90.73	91.03
PGSC0003DMT400091080	Potato	cytosol	84.98	85.26
PGSC0003DMT400066389	Potato	cytosol	76.36	81.85
PGSC0003DMT400088862	Potato	cytosol	85.94	78.2
VIT_03s0063g00740.t01	Wine grape	cytosol	53.04	51.55
VIT_03s0063g00750.t01	Wine grape	cytosol	52.72	51.08
KRH50256	Soybean	cytosol	50.48	49.69
PGSC0003DMT400020612	Potato	cytosol	48.56	47.95
CDX77920	Canola	cytosol	47.6	47.76
KRH71161	Soybean	cytosol	41.53	47.62
KRH71162	Soybean	cytosol, nucleus	50.16	47.15
AT2G03550.1	Thale cress	cytosol	46.96	47.12
Bra025117.1-P	Field mustard	cytosol	45.69	47.04
GSMUA_Achr3P06860_001	Banana	cytosol	36.74	46.94
GSMUA_Achr9P24230_001	Banana	cytosol	39.62	46.79
Bra019558.1-P	Field mustard	cytosol	48.24	46.75
AT3G48690.1	Thale cress	cytosol	48.24	46.6
CDY37916	Canola	cytosol	47.6	46.13
PGSC0003DMT400066385	Potato	cytosol	45.05	45.93
KRH50257	Soybean	cytosol	47.6	45.85
PGSC0003DMT400081445	Potato	cytosol	46.33	45.45
PGSC0003DMT400066384	Potato	cytosol	29.71	45.37
AT1G19190.1	Thale cress	cytosol	46.01	45.28
CDX83045	Canola	cytosol	46.65	45.06
GSMUA_Achr6P04230_001	Banana	cytosol	33.55	44.87
AT1G49660.1	Thale cress	cytosol	45.69	44.83
Bra029913.1-P	Field mustard	cytosol	46.65	44.79
TraesCS5D01G248500.1	Wheat	cytosol	45.05	44.48
Zm00001d025549_P001	Maize	cytosol	45.69	44.41
AT3G48700.1	Thale cress	cytosol	46.65	44.38
CDX68486	Canola	cytosol	45.69	44.27
TraesCS7B01G077500.1	Wheat	golgi	45.69	44.27
CDY20870	Canola	cytosol	45.05	44.2
GSMUA_Achr6P04360_001	Banana	cytosol	41.21	44.03
CDY41024	Canola	peroxisome	45.37	43.96
CDY67024	Canola	cytosol	45.37	43.96
CDY29339	Canola	cytosol	44.73	43.89
EER96865	Sorghum	cytosol	45.69	43.73
Zm00001d037288_P001	Maize	cytosol, extracellular	43.45	43.73
TraesCS7D01G173500.1	Wheat	cytosol	45.05	43.65
Bra040667.1-P	Field mustard	cytosol	44.41	43.57
Bra018807.1-P	Field mustard	cytosol	45.37	43.56
TraesCS5A01G243100.1	Wheat	cytosol	45.05	43.52
CDX96674	Canola	cytosol	45.05	43.38
CDY28094	Canola	cytosol	44.41	43.3
CDY51035	Canola	cytosol	45.05	43.25
KXG35781	Sorghum	cytosol	43.13	43.13
Bra016519.1-P	Field mustard	cytosol	45.05	43.12
TraesCS7A01G172600.1	Wheat	cytosol	45.05	43.12
TraesCS5B01G241000.1	Wheat	cytosol	44.41	42.9
TraesCS7D01G173400.1	Wheat	cytosol	44.41	42.9
TraesCS2D01G315300.1	Wheat	cytosol	44.09	42.86
Zm00001d020737_P001	Maize	extracellular	43.13	42.86
PGSC0003DMT400081449	Potato	cytosol	26.52	42.78
Bra029912.1-P	Field mustard	cytosol	44.09	42.72
TraesCS5A01G243000.1	Wheat	cytosol	44.09	42.59
Os06t0214800-01	Rice	cytosol	44.73	42.55
PGSC0003DMT400039929	Potato	cytosol	43.45	42.37
EER99019	Sorghum	cytosol	44.09	42.2
Zm00001d005910_P001	Maize	cytosol	44.09	42.2
Zm00001d050493_P001	Maize	cytosol, extracellular	44.09	42.2
TraesCS5A01G437300.1	Wheat	cytosol	44.73	42.17
TraesCS5D01G444200.1	Wheat	cytosol	44.73	42.17
TraesCS2D01G498000.1	Wheat	cytosol	43.13	42.06
Zm00001d020744_P001	Maize	cytosol	39.62	42.03
Bra025714.1-P	Field mustard	cytosol	31.95	42.02
HORVU5Hr1G069110.1	Barley	cytosol	43.45	41.98
HORVU5Hr1G069140.1	Barley	cytosol	43.45	41.98
KXG26418	Sorghum	cytosol	44.41	41.87
Zm00001d031805_P001	Maize	cytosol	36.1	41.7
Os09t0461700-01	Rice	cytosol	42.49	41.69
GSMUA_Achr7P18960_001	Banana	cytosol	43.13	41.67
TraesCS5D01G249600.1	Wheat	cytosol	43.13	41.67
EER88163	Sorghum	cytosol	42.17	41.64
TraesCS5B01G439900.2	Wheat	cytosol	44.41	41.62
Os08t0475400-02	Rice	cytosol, plasma membrane	43.45	41.59
TraesCS3D01G124000.1	Wheat	cytosol	41.53	41.53
KXG25323	Sorghum	cytosol	43.77	41.52
HORVU2Hr1G110800.1	Barley	cytosol	42.81	41.49
PGSC0003DMT400066383	Potato	cytosol	40.26	41.45
CDY28098	Canola	cytosol	42.49	41.3
TraesCS3B01G141200.1	Wheat	cytosol	41.53	41.27
CDY51033	Canola	cytosol	42.17	41.25
GSMUA_Achr9P24240_001	Banana	cytosol	42.81	41.23
PGSC0003DMT400077411	Potato	cytosol	43.13	41.16
EER88069	Sorghum	cytosol	43.77	41.14
Zm00001d020738_P001	Maize	plastid	43.77	41.14
TraesCS5A01G450100.1	Wheat	cytosol	41.85	41.07
TraesCS4B01G135300.1	Wheat	cytosol	41.85	40.81
Os09t0462200-01	Rice	cytosol, extracellular	43.77	40.77
KRH71160	Soybean	nucleus	51.12	40.71
Zm00001d020732_P001	Maize	plastid	40.26	40.65
AT1G49640.1	Thale cress	cytosol	40.89	40.63
Zm00001d044921_P001	Maize	cytosol, extracellular	42.81	40.61
Zm00001d020707_P001	Maize	cytosol, extracellular	37.06	40.56
GSMUA_Achr7P12950_001	Banana	cytosol	41.85	40.56
TraesCS2B01G526100.1	Wheat	cytosol	41.85	40.56
CDY02986	Canola	cytosol	45.69	40.51
Bra018809.1-P	Field mustard	cytosol	40.89	40.51
Os09t0461900-01	Rice	cytosol, plasma membrane	41.53	40.5
TraesCS5A01G242600.1	Wheat	cytosol	41.85	40.43
KXG21709	Sorghum	cytosol, mitochondrion, plastid	36.1	40.36
HORVU5Hr1G017510.2	Barley	cytosol	41.21	40.31
EES16153	Sorghum	cytosol	43.13	40.3
TraesCS5A01G242500.1	Wheat	plastid	43.77	40.29
TraesCS5B01G059800.1	Wheat	cytosol	39.94	40.19
Zm00001d016895_P001	Maize	cytosol	42.81	40.12
PGSC0003DMT400018674	Potato	cytosol	41.21	40.06
PGSC0003DMT400018589	Potato	cytosol	32.27	39.92
TraesCS6B01G046900.1	Wheat	mitochondrion	29.07	39.91
TraesCS5D01G068400.1	Wheat	cytosol	39.62	39.87
TraesCS4B01G331200.1	Wheat	cytosol	40.89	39.75
Os09t0455500-01	Rice	cytosol	40.58	39.69
PGSC0003DMT400081446	Potato	cytosol	41.53	39.63
OQU81663	Sorghum	cytosol	45.05	39.61
TraesCS2A01G497900.1	Wheat	cytosol	40.58	39.44
TraesCS6B01G047900.1	Wheat	cytosol	40.89	39.38
TraesCS5B01G240000.1	Wheat	cytosol	40.58	39.32
Os09t0460500-00	Rice	cytosol	39.3	39.3
TraesCS2A01G317400.1	Wheat	mitochondrion, plastid	44.41	39.27
TraesCS6A01G033400.1	Wheat	cytosol	40.89	39.26
TraesCS2D01G389600.1	Wheat	cytosol	40.58	39.2
TraesCS6D01G038600.1	Wheat	cytosol	40.58	39.08
TraesCS7D01G173800.1	Wheat	golgi	39.3	39.05
OQU85176	Sorghum	plastid	42.49	39.0
OQU89622	Sorghum	cytosol	39.62	38.99
TraesCS5D01G249100.1	Wheat	cytosol	38.66	38.91
Zm00001d050837_P001	Maize	cytosol	41.21	38.86
PGSC0003DMT400081451	Potato	cytosol	40.58	38.84
EER89413	Sorghum	cytosol	42.17	38.82
HORVU6Hr1G005900.1	Barley	cytosol	39.94	38.82
Os06t0214300-01	Rice	plasma membrane, plastid	39.94	38.82
TraesCS4D01G328100.1	Wheat	cytosol	39.94	38.82
Zm00001d020740_P001	Maize	cytosol	40.89	38.79
Os09t0460700-01	Rice	cytosol	38.66	38.78
TraesCS5A01G502600.1	Wheat	cytosol	39.62	38.75
TraesCS5D01G249700.2	Wheat	cytosol	43.77	38.59
HORVU2Hr1G093140.1	Barley	cytosol	39.94	38.58
Os02t0567800-01	Rice	cytosol	39.3	38.44
PGSC0003DMT400081450	Potato	cytoskeleton, cytosol, nucleus	40.26	38.41
HORVU0Hr1G005140.1	Barley	extracellular	38.66	38.29
KXG19591	Sorghum	cytosol	39.62	38.27
TraesCS7A01G172500.1	Wheat	peroxisome	39.62	38.27
Os09t0455900-01	Rice	cytosol, plasma membrane	38.98	38.24
Os09t0460400-01	Rice	cytosol	38.34	38.1
TraesCS5B01G240200.1	Wheat	endoplasmic reticulum, vacuole	41.85	38.08
AT1G49650.1	Thale cress	mitochondrion	45.37	37.97
HORVU5Hr1G068140.1	Barley	cytosol	39.3	37.96
TraesCS7B01G077300.1	Wheat	peroxisome	39.3	37.96
TraesCS5A01G242000.1	Wheat	cytosol	38.02	37.9
TraesCS5A01G065200.1	Wheat	cytosol, plastid	42.49	37.89
HORVU5Hr1G069040.1	Barley	endoplasmic reticulum, vacuole	41.85	37.86
Zm00001d037180_P001	Maize	cytosol, extracellular	39.3	37.85
HORVU0Hr1G019700.1	Barley	cytosol, mitochondrion	29.07	37.76
TraesCS5D01G248700.1	Wheat	endoplasmic reticulum, vacuole	41.53	37.68
TraesCS5B01G235400.1	Wheat	cytosol	38.98	37.65
KXG25394	Sorghum	cytosol	39.62	37.58
Zm00001d020736_P001	Maize	endoplasmic reticulum, vacuole	41.53	37.57
TraesCS2B01G409800.1	Wheat	cytosol	39.94	37.54
TraesCS5A01G242300.1	Wheat	extracellular, golgi	41.53	37.46
TraesCS2B01G053400.1	Wheat	cytosol	41.21	37.39
KXG35782	Sorghum	cytosol	40.26	37.39
TraesCS5D01G076000.1	Wheat	cytosol	41.85	37.32
TraesCS5D01G249000.1	Wheat	cytosol	37.38	37.26
TraesCS5D01G248900.1	Wheat	cytosol	39.94	37.2
TraesCS5B01G072000.1	Wheat	cytosol, mitochondrion	41.53	37.14
TraesCS5A01G242100.1	Wheat	cytosol	39.94	37.09
TraesCS5D01G244200.1	Wheat	cytosol	38.34	37.04
CDY32845	Canola	mitochondrion	43.13	36.89
Zm00001d020742_P001	Maize	cytosol	33.87	36.81
TraesCS2B01G335600.1	Wheat	plastid	44.09	36.8
Os09t0461500-01	Rice	golgi, mitochondrion	40.89	36.78
TraesCS4A01G437700.1	Wheat	cytosol	29.39	36.65
CDY16521	Canola	mitochondrion	44.41	36.58
Bra018808.1-P	Field mustard	plastid	44.41	36.48
KXG35780	Sorghum	extracellular, vacuole	41.21	36.44
Bra014213.1-P	Field mustard	mitochondrion	44.09	36.32
CDY28095	Canola	plastid	44.09	36.32
CDY51034	Canola	plastid	44.09	36.32
TraesCS5B01G240500.1	Wheat	cytosol	36.74	36.28
Os08t0474866-00	Rice	extracellular	42.81	36.22
TraesCS5B01G412100.1	Wheat	endoplasmic reticulum	38.66	36.12
TraesCS2D01G389500.1	Wheat	cytosol	34.82	36.09
Zm00001d005905_P001	Maize	endoplasmic reticulum, golgi	40.58	36.08
Zm00001d031804_P001	Maize	cytosol	37.7	35.98
TraesCS5D01G417100.1	Wheat	endoplasmic reticulum	38.66	35.91
KXG35760	Sorghum	cytosol	37.06	35.8
KXG35783	Sorghum	extracellular, vacuole	41.21	35.54
PGSC0003DMT400094975	Potato	cytosol, extracellular, plastid	30.99	35.53
Zm00001d005909_P001	Maize	plasma membrane	40.89	35.46
TraesCS7D01G173300.1	Wheat	extracellular	40.26	35.29
TraesCS4D01G130000.1	Wheat	extracellular, vacuole	41.85	35.22
Os06t0214850-00	Rice	plasma membrane	39.3	34.94
TraesCS5B01G240400.1	Wheat	mitochondrion	37.7	34.91
HORVU3Hr1G022640.1	Barley	mitochondrion	40.58	34.79
OQU80639	Sorghum	cytosol	37.06	34.73
TraesCS5B01G240800.1	Wheat	extracellular, golgi	40.58	34.51
HORVU5Hr1G093500.4	Barley	plastid	40.89	34.5
KXG35761	Sorghum	cytosol	35.78	34.36
Zm00001d020741_P001	Maize	golgi, mitochondrion, vacuole	41.53	34.3
Zm00001d050479_P001	Maize	extracellular, plasma membrane, vacuole	41.53	34.3
Os08t0475100-01	Rice	extracellular	36.42	34.23
Os09t0462100-01	Rice	plasma membrane	41.53	34.21
EES15127	Sorghum	extracellular	40.89	34.13
TraesCS2B01G381300.1	Wheat	vacuole	42.81	34.1
Zm00001d031801_P001	Maize	endoplasmic reticulum	38.66	34.08
TraesCS5A01G242800.1	Wheat	extracellular, plasma membrane	40.26	34.05
HORVU5Hr1G069090.1	Barley	endoplasmic reticulum, golgi	40.26	33.78
TraesCS2D01G360900.1	Wheat	extracellular	42.49	33.76
KXG35785	Sorghum	extracellular, golgi, vacuole	40.58	33.69
TraesCS5D01G249400.1	Wheat	endoplasmic reticulum, plasma membrane	39.94	33.42
HORVU0Hr1G013180.1	Barley	endoplasmic reticulum, golgi, mitochondrion	40.26	33.42
OQU80637	Sorghum	plasma membrane	42.17	33.33
TraesCS5D01G249500.1	Wheat	endoplasmic reticulum, golgi	40.26	33.25
EES14010	Sorghum	endoplasmic reticulum, extracellular, vacuole	37.7	33.24
HORVU0Hr1G013170.1	Barley	cytosol	34.82	33.03
TraesCS5B01G240700.1	Wheat	endoplasmic reticulum, golgi	39.62	32.98
TraesCS7D01G046800.1	Wheat	cytosol	38.66	32.97
OQU89623	Sorghum	mitochondrion	39.94	32.72
TraesCS5A01G242900.1	Wheat	extracellular, golgi	39.94	32.72
HORVU0Hr1G013160.1	Barley	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, plasma membrane, vacuole	27.16	32.69
TraesCS5B01G239300.1	Wheat	plastid	39.62	32.63
HORVU5Hr1G068800.1	Barley	plastid	39.3	32.37
HORVU5Hr1G098770.1	Barley	plasma membrane	37.38	32.32
HORVU2Hr1G002620.1	Barley	cytosol	41.85	31.95
TraesCS4A01G183300.2	Wheat	extracellular, golgi	42.17	31.73
TraesCS2B01G006100.1	Wheat	cytosol	41.53	31.63
TraesCS2B01G006500.1	Wheat	cytosol	41.53	31.63
TraesCS2D01G015900.1	Wheat	cytosol	41.53	31.63
TraesCSU01G240900.1	Wheat	cytosol	41.53	31.63
TraesCS5D01G247700.1	Wheat	plastid	38.66	31.43
TraesCS5A01G241200.1	Wheat	plastid	38.34	31.17
TraesCS4A01G436500.1	Wheat	cytosol	38.02	30.91
Os09t0460300-01	Rice	plastid	38.02	30.75
TraesCSU01G158400.1	Wheat	cytosol	38.34	30.3
TraesCS4A01G436300.1	Wheat	cytosol	37.7	30.18
TraesCS4A01G438000.1	Wheat	cytosol	19.49	29.76
Os08t0475000-00	Rice	cytosol	32.27	29.45
TraesCS4A01G437800.1	Wheat	cytosol	38.34	28.71
HORVU0Hr1G004410.1	Barley	cytosol	39.94	28.6
TraesCS7A01G093800.1	Wheat	cytosol, plastid	39.3	28.47
TraesCS4A01G436100.1	Wheat	cytosol	38.66	28.47
TraesCS5A01G241700.1	Wheat	plastid	34.19	28.46
HORVU7Hr1G009170.1	Barley	cytosol	38.34	28.44
TraesCS4A01G393200.1	Wheat	cytosol	39.3	28.41
TraesCS5A01G407200.1	Wheat	plastid	38.66	28.4
TraesCS5D01G248100.1	Wheat	plastid	34.19	28.38
HORVU0Hr1G013200.3	Barley	plastid	33.87	28.34
TraesCS7D01G089100.1	Wheat	cytosol	38.98	28.18
TraesCS7D01G046600.1	Wheat	cytosol	38.34	28.1
OQU76051	Sorghum	golgi, plasma membrane, vacuole	43.13	27.84
TraesCS2A01G363400.1	Wheat	endoplasmic reticulum, extracellular, vacuole	35.78	27.52
EER97903	Sorghum	cytosol	39.62	27.37
TraesCS7D01G046900.1	Wheat	mitochondrion	39.3	27.27
TraesCS2A01G021800.1	Wheat	cytosol	38.34	26.85
TraesCS2B01G007100.1	Wheat	cytosol	38.02	26.62
TraesCS1B01G060800.1	Wheat	cytosol	37.7	26.58
TraesCS5B01G239700.1	Wheat	plastid	34.19	26.16
HORVU2Hr1G067270.2	Barley	cytosol	35.14	26.13
HORVU5Hr1G068960.1	Barley	cytosol	35.14	26.13
TraesCS2D01G051400.1	Wheat	cytosol	37.7	26.11
TraesCSU01G143300.1	Wheat	cytosol	36.74	25.9
Os07t0501400-00	Rice	plastid	37.06	25.89
TraesCS2D01G022400.1	Wheat	cytosol	37.7	25.88
TraesCSU01G008500.1	Wheat	cytosol	37.7	25.88
Os07t0526600-01	Rice	extracellular	37.06	25.22
HORVU2Hr1G003300.1	Barley	cytosol	38.02	24.95
Os09t0435700-00	Rice	mitochondrion	32.27	23.71
GSMUA_Achr5P24460_001	Banana	cytosol, endoplasmic reticulum, peroxisome	39.62	20.13
TraesCS3D01G500400.1	Wheat	cytosol	3.51	6.88

Protein Annotations

EnsemblPlants:PGSC0003DMT400066392	EnsemblPlantsGene:PGSC0003DMG400025819	EntrezGene:102579845	Gene3D:3.40.50.1820	GO:GO:0003674	GO:GO:0003824
GO:GO:0008150	GO:GO:0008152	GO:GO:0016787	InterPro:AB_hydrolase	InterPro:AB_hydrolase_3	InterPro:IPR029058
PANTHER:PTHR23024	PANTHER:PTHR23024:SF251	PFAM:PF07859	PGSC:PGSC0003DMG400025819	RefSeq:XP_006346462.1	SUPFAM:SSF53474
UniParc:UPI0002960D50	UniProt:M1CFH8	MapMan:35.1	:	:	:

Description

Gibberellin receptor GID1 [Source:PGSC_GENE;Acc:PGSC0003DMG400025819]

Coordinates

chr1:+:84934867..84936222

Molecular Weight (calculated)

34908.8 Da

IEP (calculated)

4.910

GRAVY (calculated)

-0.108

Length

313 amino acids

Sequence
(BLAST)

001: MEEIVHDFFP FMRVYKDGRI VRLMGEGFVP PESDPETGVQ IKDVEIDPQI NLSARLYLPK NVDSVQKIPL FVYFHGGGFV IESASSPSYH KHISKVAAEA
101: KVVIVSVNYR LAPEYLLPIA YEDSWLALKW VASHIANGDG DEPWLKDHAD FNRVYLGGDS AGGNIAHHIA IRVGLEKLDG MKLEGIFLAC PFFWGKDPID
201: GEGENLGSKD VIEKLWLFVN PNSSGLDDPL INPEKDPKLC SLGCDKVLVY VAGKDTLRFR GFYYKEVLEK SGWPGTVEIV EVKDEAHVFY LSATEAENAM
301: AMIKKLASFL NQS

Best Arabidopsis Sequence Match ( AT2G03550.1 )
(BLAST)

001: MDSVIAFDRS PMFRVYKSGR IERLLGETTV PPSLTPQNGV VSKDIIHSPE KNLSLRIYLP EKVTVKKLPI LIYFHGGGFI IETAFSPPYH TFLTSAVAAA
101: NCLAISVNYR RAPEFPVPIP YEDSWDSLKW VLTHITGTGP ETWINKHGDF GKVFLAGDSA GGNISHHLTM RAKKEKLCDS LISGIILIHP YFWSKTPIDE
201: FEVRDVGKTK GVEGSWRVAS PNSKQGVDDP WLNVVGSDPS GLGCGRVLVM VAGDDLFVRQ GWCYAEKLKK SGWEGEVEVM ETKNEGHVFH LKNPNSDNAR
301: QVVKKLEEFI NK

Arabidopsis Description

CXE7Probable carboxylesterase 7 [Source:UniProtKB/Swiss-Prot;Acc:Q9ZQ91]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.